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Introduction
In 1833, the mathematician and cleric William Foster in modern medicine, as well as our ability to treat
Lloyd published his lecture entitled “Two Lectures on the commonly encountered infectious diseases.
Checks to Population”.1 He described how farmers using Urinary tract infections (UTIs) are among the most
common grazing areas for their sheep could deplete this common types of infectious disease, with approximately
The University of
Queensland, UQ Centre shared resource to the eventual detriment of all, while 150–250 million cases globally per year.4–6 About 40–50%
for Clinical Research, still acting in rational self-interest. 1 The economist of women and 5% of men will develop a UTI at least once
Building 71/918 Royal
Brisbane Hospital,
Garret Hardin later encapsulated this concept as “the during their lifetime.5,7 In the USA alone, the attributed
Herston, QLD 4006, tragedy of the commons”.2 A similar dilemma could be cost of UTI-related medical expenses has been estimated
Australia (H.M.Z., said to have arisen from our use of antibiotics. In seeking to range from US$1.6 billion to $3.5 billion annually if
P.N.A.H., M.J.R., D.L.P.).
Division of Infectious to maximize benefit for individual patients, clinicians broader societal costs are also considered.5,8–10 Owing
Diseases, National now risk diminished utility of this precious shared to their high prevalence, UTIs are a major contributor to
University Health
System, 1E Kent Ridge resource for the many. global antibiotic use and resistance.11,12 Without effec-
Road, 119228, Bacterial resistance to our antibiotic arsenal is not a tive antibiotics active against common uropathogens,
Singapore (P.A.T.).
School of Chemistry
new phenomenon. Indeed, multiple resistance deter- many urological procedures would carry excessive risk.
and Molecular minants have been found in bacteria isolated from Accordingly, this Review aims to summarize the current
Biosciences, environments that have been separated from human global epidemiology of resistance in Gram-negative
The University of
Queensland, Brisbane, activity for millions of years.3 However, the antimicro- uropathogens, describe the genetic and molecular
QLD 4072, Australia bial resistance crisis we currently face reflects the rapid mechanisms underlying the resistance of these pheno
(M.A.S.). Department of
Biomedical and Clinical
expansion, diversification and extension of host range types, examine the effect of resistance on common
Sciences L. Sacco, for a multitude of resistance determinants under selec- urological procedures and discuss therapeutic and
University of Milan, tion pressure from the widespread use of antibiotics. preventive options.
G. B. Grassi 74,
20157 Milan, Italy This man-made crisis threatens many of the advances
(M.D.P.). Department Key resistance mechanisms
of Pathology,
University of Otago,
For many years, resistance profiles for common Gram-
Competing interests
23A Mein Street, P.A.T. has received research support from ADAMAS, Baxter, negative uropathogens such as Escherichia coli or
Newtown,
Fabentech, Inviragen, Merlion Pharmaceuticals and Sanofi Klebsiella pneumoniae were relatively predictable and
Wellington 6242,
New Zealand (D.A.W.).
Pasteur, and has received honoraria from AstraZeneca stable over time. In the past few decades, however, this
and Novartis. D.L.P. has participated in advisory boards and
picture has changed dramatically. During the 1980s,
Correspondence to: received honoraria from AstraZeneca, Bayer, Cubist,
H.M.Z. Leo Pharmaceuticals, Merck and Pfizer. The other authors we witnessed the emergence of extended-spectrum
h.zowawi@uq.edu.au declare no competing interests. β‑lactamases (ESBLs) in Enterobacteriaceae. ESBLs are
Contain serine residues ESBLs usually arise from mutations in ‘parent’ ESBLs are most common in ESBLs: TEM and SHV
Key features of ESBLs are narrow-spectrum β‑lactamase or have been Escherichia coli, Klebsiella spp. variants, CTX‑M
cephalosporinase activity ‘captured’ from environmental bacteria and Proteus spp., but have Carbapenemase: KPC
and resistance to 3GCs (e.g. CTX‑M from Kluyvera spp.) been described in most
Usually inhibited by clavulanate– Transmissible on mobile genetic elements, Enterobacteriaceae and
tazobactam in vitro (except KPC) such as plasmids, carrying multiple other in Pseudomonas spp.
resistance determinants KPC is seen in K. pneumoniae
Production of class B* β‑lactamases‡
Contain metal ions (for example, Zn2+) Usually highly transmissible on plasmids E. coli, Klebsiella spp. but described Carbapenemase:
Have carbapenemase activity, not inhibited carrying multiple other resistance determinants in many Enterobacteriaceae and IMP, NDM, VIM
by clavulanate/tazobactam Acinetobacter spp.
Aztreonam not hydrolysed by class B Intrinsic carbapenem resistance
β‑lactamases in Stenotrophomonas maltophilia
via class B enzyme (L‑1)
Production of class C* β‑lactamases‡
Also known as ‘AmpC’ enzymes Chromosomally encoded in many species, Enterobacter cloacae, E. aerogenes, Cephalosporinase:
Broad cephalosporinase activity but can also be inducible in Serratia marcescens, Citrobacter CMY, DHA, ACT
including 3GCs, but cefepime stable some Enterobacteriaceae freundii, Pseudomonas aeruginosa,
Not inhibited effectively by clavulanate Mutations in regulatory genes (for example, Providencia spp. and Morganella
or tazobactam ampD or ampR) involved in cell-wall recycling morganii all contain inducible
can lead to high-level AmpC expression and AmpC enzymes that are
resistance to a broad range of β‑lactams chromosomally encoded
Increasing plasmid-AmpC Plasmid-mediated AmpC (e.g. CMY)
transmission described increasing in E. coli
Production of class D* β‑lactamases‡
Oxacillinases that can have Can be acquired or naturally occurring Acinetobacter baumanii Carbapenemase:
carbapenemase activity and are only chromosomal genes (e.g. OXA‑23), Enterobacteriaceae OXA-type
weakly inhibited by clavulanate (e.g. OXA‑48), Pseudomonas spp.
Efflux pumps and porin mutations
Membrane transport systems to extrude Poly-specific transporters; for example, the Pseudomonas aeruginosa, Efflux: AcrAB-like
multiple antimicrobials or mutations in resistance-nodulation-division (RND) family Acinetobacter baumanii, or MexAB-OprM
outer membrane proteins to hinder entry Porin changes or loss via mutation Enterobacteriaceae Porin: OprD channel
of active drug (e.g. Enterobacter spp.) loss
Target site mutations
Methylation of 16S rRNA with high-level Carried by plasmids, often mediated by rmtA Enterobacteriaceae Methylases: RmtA,
resistance, including against amikacin and related genes (e.g. K. pneumoniae) RmtB or ArmA
Altered DHPS—essential for folate sul1 gene part of class 1 integron—frequently Stenotrophomonas maltophilia, E. coli sul1 and dhrf genes
synthesis in bacteria—leads to integrated into plasmids
sulphonamide resistance and altered DHFR Plasmid-borne dhfrI and dhfrII genes
with loss of inhibition by trimethoprim
DNA gyrase mutations prevent activity Often single mutations in quinolone resistance- Pseudomonas aeruginosa, Point mutations in
of quinolones determining region (QRDR) on gyrA gene Salmonella spp., other gyrA, gyrB, parC
Enterobacteriaceae and parE genes
Protein binding of quinolone active site, Protein binding encoded by plasmid-mediated E. coli, Klebsiella spp., Salmonella qnrA, qnrB, qnrC,
low-level resistance qnr genes spp. qnrD and qnrS
Overproduction of enzymes
Sulphonamide or trimethoprim resistance felP gene produces DHPS and folA encodes E. coli Mutations in
by overproduction of DHPS or DHFR DHFR promotor regions
result in increased
production of DHFR
and trimethoprim
resistance
Drug modification
Acetylation, nucleotidylation or Often carried on transmissible elements such Pseudomonas aeruginosa AAC(2’), ANT(2”),
phosphorylation of aminoglycosides as plasmids or transposons and Enterobacteriaceae APH(2”)
Some can also inactivate fluoroquinolones Can be chromosomal in some species Intrinsic to Providencia spp. AAC(6’)-lb-cr (also
confers quinolone
resistance)
*Ambler Classification Scheme based on amino acid sequence and protein structure. ‡β-lactamase enzymes hydrolyse the β‑lactam ring and inactivate the drug. Abbreviations: 3GC,
third‑generation cephalosporins; AAC, aminoglycoside acetyltransferase; ANT, aminoglycoside nucleotidyltransferase; APH, aminoglycoside phosphotransferase; DHFR, dihydrofolate reductase;
DHPS, dihydropteroic acid synthase; ESBLs, extended-spectrum β-lactamases; KPC, Klebsiella pneumoniae carbapenemase.
a
Mutation in Outer Inner Overexpression
lipopolysaccharide membrane membrane of efflux pumps
(polymixin resistance) (multidrug resistance)
Antibiotic
Periplasmic Porin
space Plasmid
Modified drug target
(e.g. quinolones)
Hydrolysis by β-lactamase
(plasmid-acquired Porin loss
or overexpressed Cell wall or mutation
chromosomal genes) (peptidoglycan) Antibiotic
b Luminal replication
and attachment of
fimbriated UPEC
Attachment Invasion
Superficial
bladder
epithelial cell
Transitional
epithelial cell
Filamentation Intracellular bacterial
and exfoliation community formation
Quiescent
intracellular
reservoir
c
Antibiotic
Planktonic
bacteria
Exopolymer
Urinary production
catheter
Prostate Bladder
Rectum
◀ Figure 1 | Infection and resistance in urological practice. a | Antibiotic resistance facilities.51 Such infections are associated with a cost of
mechanisms in Gram-negative bacteria. These include antibiotic hydrolysis by
approximately US$500 per episode52 and, especially in
β‑lactamase enzymes (including carbapenemases) or other antibiotic-modifying
enzymes (for example, against aminoglycosides). Porin loss or mutation can
situations in which urinary catheter obstruction has
reduce antibiotic permeability; increased expression or activity of efflux pumps can occurred, have associated risks of morbidity and mortal
prevent an antibiotic reaching its target site; a modified drug target can stop ity.53 Indwelling urinary catheters have been associated
antibiotics (for example, quinolones) binding to the active site; mutation in with an increased risk of community and nosocomial
lipopolysaccharide can render the bacteria resistant to the polymyxin group of UTIs, including infections caused by ESBL-producing 54,55
antibiotics. b | UPEC pathogenesis. UPEC attach to and invade uroepithelial cells and carbapenem-resistant 56 organisms.
and form intracellular bacterial communities. These aid evasion of the host Patients with indwelling urinary catheters are a major
immune system and provide some protection against antibiotic exposure. The
reservoir of antimicrobial-resistant uropathogens in
formation of a quiescent reservoir within the transitional cell layer might contribute
to relapsing or persistent infection. c | Biofilm formation enables the organism to health-care settings.57 Such patients are often elderly
avoid host defences, resist antibiotic therapy and provides a reservoir for ongoing and have comorbidities and many of them are residents
infection if the catheter is not removed. d | Transrectal prostate biopsy allows of care facilities. In addition, patients with indwelling
direct transfer of bacteria from the rectum to the prostate, including a potential urinary catheters are often exposed to antibiotics and
reservoir of resistance that can be enhanced by prior antibiotic exposure. are at risk of cross-transmission of multidrug-resistant
Abbreviation: UPEC, uropathogenic E. coli. pathogens. Once colonized, catheterized patients are
vulnerable to both symptomatic CAUTI and long-term
their ability to cause disease, including adhesins, toxins, colonization. The indwelling urinary catheter bypasses
flagella, surface polysaccharides (for example, various the normal host defences along the urethra, giving micro
types of O antigen and capsule) and iron-acquisition organisms that colonize the perineum direct access to the
systems that utilise siderophores (for example, entero- bladder.58 In the past, when catheter drainage was open,
bactin).5,40,41 Many of these virulence factors have been with urine collected in a drainage container, bacteriuria
extensively characterized in mouse models of UTI, and was almost universal within 1 week.59 In the 1970s, closed
are strongly associated with UPEC strains isolated from catheter drainage was one of the biggest advances in the
UTI patients.9,40 prevention of CAUTI; however, breaks in the collection
The best-defined UPEC virulence factors are fim- system do occur,60 leading to colonization of the drainage
briae,42,43 of which multiple different types can be pro- bag and intraluminal entry of bacteria into the catheter-
duced.44 Type 1 fimbriae, as an example, confer the ability ized urinary tract. Bacteria can also enter the bladder
to bind to α‑D-mannosylated glycoproteins such as uro- through the extraluminal route along the external surface
plakins in the bladder, and in animal infection models of the catheter. In this situation, organisms ascend into
promote enhanced colonization, induction of host the bladder from the perineum through the biofilm
responses, bladder cell invasion and biofilm develop that forms rapidly on the external surface.61 In a large
ment through the formation of intracellular bacterial prospective study of 1,497 newly catheterized patients,
communities.40,45,46 Fimbriae-mediated adhesion is also the intraluminal route of entry was as common as the
an important virulence characteristic of most other uro- extraluminal route along the external surface of the
pathogens; for example, Proteus mirabilis also has the catheter for Gram-negative bacterial infection, whereas
potential to produce several types of fimbriae, some of Gram-positive bacterial infection was more frequently
which contribute to UTI.47 In addition to adherence, bac- associated with colonisation via the extraluminal route.61
terial pathogens that successfully colonize the urinary Once microorganisms gain entry into the catheterized
tract and cause UTI must possess a virulence arsenal that urinary tract, they form a biofilm on both surfaces of the
enables them to avoid host-killing mechanisms such as catheter (Figure 1c). Such biofilms become encased in a
antimicrobial peptides and cytokines, neutrophil influx, polymeric matrix that provides protection against host
inflammation, apoptosis and exfoliation of host cells.5,41 immune defences and enhanced resistance to most anti-
UPEC can evade host responses through the develop- microbial agents.62 Attempts have been made to prevent
ment of intracellular bacterial communities as a pro- the adherence of organisms to the catheter surface. These
tected intracellular niche within epithelial cells during attempts have included the use of hydrophilic surfaces63
the early acute stages of UTI, and through prolonged sur- and the modification of the surface through use of anti-
vival in quiescent intracellular reservoirs that may serve microbial agents or antiseptics. The most commonly
as a reservoir for recurrent infection (Figure 1b).40,48 used agents to coat the surface of the catheter have been
silver or nitrofural, but such modifications have yielded
Antibiotic resistance in urology limited benefits. 64,65 A randomized trial of impreg-
Antibiotic resistance has a number of implications nated catheters showed a limited reduction in CAUTI
for urological practice, including the treatment of in patients with nitrofural-coated catheters compared
catheter-associated urinary tract infections (CAUTIs) with patients with standard polytetrafluoroethylene-
and treatment of infections associated with transrectal- coated catheters, but this finding was not seen as clini-
ultrasonography-guided biopsy of the prostate (TRUBP). cally significant, and was associated with greater patient
discomfort and an increased requirement for catheter
Catheter-associated UTIs removal.66 Currently, no international guidelines rec-
CAUTIs are the most common nosocomial infections ommend the routine use of catheters coated with anti-
suffered by patients in hospitals49,50 and long-term care microbial agents,67,68 although such catheters can be
Prevalence (%)
0 10 20 30 40 50 60 70 80
Figure 2 | Global epidemiology of resistance in Gram-negative uropathogens—fluoroquinolones. Prevalence of resistance
Nature Reviews | Urology
to fluoroquinolones in Gram-negative urinary pathogens by country. Data obtained from studies published 2009–2014.
The accuracy of these prevalence estimates is affected by the number and heterogeneity of studies undertaken in each
country, and reflects resistance data from clinical isolates, which only represent a subset of the total resistance burden
in colonized patients.
considered if the CAUTI rate has not declined after the TRUBP-associated infections
implementation of a comprehensive strategy aimed at Incidence and clinical context
reducing infections.69 TRUBP is commonly used to diagnose prostate cancer.
Once the biofilm is established, eradication of the This method is time-efficient, cost-effective and is often
infection is very difficult, which exacerbates the problem performed under local anaesthesia, with minimal adverse
of antimicrobial resistance. Attempts to treat CAUTI effects that are mostly self-limiting.73,74 Periprocedural
without catheter removal almost invariably result in the antimicrobial therapy has been shown to reduce the
selection of more-resistant organisms.70 This situation is risk of infection, and is the current standard of care.75
particularly likely in individuals with a long-term indwell- Infection complicating TRUBP can cause cystitis, pros-
ing catheter owing to neurogenic bladder.70 The most tatitis, sepsis and very rarely, disseminated abscess forma-
effective approaches to prevent CAUTI and subsequent tion, mostly caused by Gram-negative Enterobacteriaceae
biofilm formation involve a reduction in the duration of such as E. coli.74,76,77 Infection after TRUBP is likely to
catheterization.71 Such approaches have included the use occur via the direct inoculation of bacteria that exist
of electronic or nurse-driven reminder systems72 and have naturally in the rectum into the prostate, urinary tract or
been shown to be effective in various settings. By contrast, local vasculature (Figure 1d).78
strategies using antiseptic irrigation of the catheterized In the past decade, an increase in hospital admission
bladder, antiseptic lubricants or topical therapies for rates secondary to TRUBP-related infection has been
meatal care have not been found to be effective.58 reported.74,79,80 The incidence of infectious complications
Although no evidence is available from randomized following TRUBP varies between regions, with emer-
controlled trials, the implementation of robust infection gency department presentations ranging from 0% to 6%,
control policies and reminders to shorten the duration hospitalization rates of up to 4% and severe sepsis rates
of catheterization are widely believed to be important ranging from 0% to 0.6%.77,81,82 A consistent theme of
in preventing CAUTI. In addition, close attention to TRUBP-associated infections is antimicrobial resistance,
aseptic technique and adherence to principles of good specifically fluoroquinolone resistance, with or without
hand hygiene are essential in reducing device-associated concurrent ESBL production.82–85 Fortunately, most iso-
infections.67–69 Prevention of CAUTI would be an impor- lates remain sensitive to aminoglycosides (particularly
tant step in reducing the reservoir of nosocomial MDR amikacin) and carbapenems. Despite the risk of infectious
Gram-negative organisms in hospitals and nursing complications and antimicrobial resistance, the 30-day
homes worldwide. mortality related to post-TRUBP sepsis is low (0.1–1%).79,80
Prevalence (%)
0 10 20 30 40 50 60 70
For urologists, this concerning trend of resistance to recommended by international urology bodies.75,95 The
antimicrobials has broad implications for prostate cancer clinical implementation of periprocedural TRUBP anti-
control, with over 1 million TRUBPs performed annually microbial therapy is varied, with different classes of agents
in the USA alone.74 Diagnosis of prostate cancer on initial and durations used, as well as use of bowel preparation
biopsy is poor at <30%, with repeat biopsy (up to four) agents.96 Oral fluoroquinolones are the most commonly
often performed owing to ongoing high clinical suspicion recommended agent, owing to a high bioavailability
of prostate cancer.86 Furthermore, active surveillance pro- (70–99%), with peak serum concentrations achieved
tocols, requiring prostate biopsy every 3 years, have been within 1 h, and good prostatic tissue penetration. In addi-
promoted as a method of managing low-volume prostate tion, high tissue:serum concentration ratios (~150%)
cancer.87,88 A North American study in 591 consecutive have been reported.77,97 This relationship has also been
men undergoing TRUBP, mostly for active surveillance, reported for trimethoprim,77 but this ratio is lower for
reported an increased risk of an infection of 1.3 (odds β‑lactams (10–20%) and amikacin (50%).77,98 Alternative
ratio [OR] 1.33, 95% CI 1.01–1.74) for every previous recommended antimicrobials (in the presence of peni-
biopsy.89 TRUBP complications cause a considerable cillin hypersensitivity) include aminoglycosides and
burden on health-care systems. A mean readmission cost metronidazole or clindamycin.99 Other first-line alterna-
of $5,900 following a TRUBP-associated infection was tives include first‑, second- and third-generation cephalo
reported from a hospital in Texas, USA.90 A UK model sporins (American Urological Association Guidelines99)
(based on severe infection with a median stay of 14.2 days) and trimethoprim–s ulfamethoxazole (European
described a cost of £4,260 per readmission, totalling Association of Urology Guidelines75). The duration of
£7.7–£11.1 million per year.91 Although this figure might TRUBP antimicrobial use varies.96 Current evidence sug-
be an overestimation in the context of TRUBP-associated gests that prolonged duration of antimicrobial use shows
infections, for which the median length of stay is in the no beneficial effect over a single dose.74,94
range 1–4 days,79,92,93 the economic burden must be con-
sidered together with the unmeasurable psychological TRUBP prophylaxis in the era of resistance
burden, which is scarcely documented in this context.76 With increasing concern surrounding a rise in infec-
tious complications following TRUBP, and in the
Current TRUBP prophylaxis strategies context of increasing antimicrobial resistance, the use
Periprocedural antimicrobial therapy has been shown of broader spectrum antimicrobial prophylaxis before
to reduce TRUBP-associated infections, 94 and is TRUBP has been suggested. The addition of amikacin to
Prevalence (%)
0 5 10 15 20 25 30 35
empirical fluoroquinolone-based regimens (in compari- than for fluoroquinolones (41.9% versus 69.2%).105,106
son to fluoroquinolone-based regimens alone) has been Modelling based upon fosfomycin trometamol concen-
reported to result in fewer TRUBP infectious complica- trations measured in prostate tissue suggests that the
tions, with a reduction from 8% to 1.7% over a 5‑year optimal dosing of fosfomycin trometamol prophylaxis
period in a North American study.100 Similar findings is 1–4 h prior to surgery and that such treatment might
were reported from a UK study, where the addition of be ineffective if the organism minimum in hibitory
amikacin over a 2‑year period reduced bacteraemia concentration (MIC) is >4 mg/l.102
rates from 2.5% to 0.3%.101 However, despite the use of Pivmecillinam, a synthetic penicillin, has been used
ciprofloxacin (a fluoroquinolone) and amikacin pro- extensively for outpatient treatment of UTI for 15 years,
phylaxis, a consistent 2.1% emergency admission rate and since 2011, has been investigated for use against
as a result of infectious sequelae was reported in one ESBL-producing organisms in vitro.107 Administration
study, with all E. coli bacteraemia isolates being resis- of carbapenems in addition to standard prophylaxis
tant to ciprofloxacin, but susceptible to amikacin.93 Oral prior to TRUBP in high-risk patients has been reported
fosfomycin trometamol, given as a 3 g dose, could be to reduce the rate of infection to below that seen in
a potential option for the prolonged outpatient treat- low-risk patients treated with standard therapy.108 Nine
ment of TRUBP-associated infections.76 Fosfomycin tro- TRUBP patients in whom MDR E. coli was detected via
metamol achieves reasonable prostatic concentrations, a rectal swab culture were given ertapenem in an out-
although these concentrations are lower in the periph- patient setting before TRUBP and no infectious com-
eral zone,102 where most prostate cancer is located,103 plications occurred.109 Although the need to reduce the
possibly reflecting differences in vascularity in differ- incidence of TRUBP-associated infections might be met
ent regions of the prostate.104 Both nonrandomized and by use of broad-spectrum agents such as carbapenems,
randomized prospective studies comparing oral fosfo- the potential for subsequent development of resistance
mycin trometamol with fluroquinolones have shown to these agents and transmission is concerning. Even
these agents to have equivalent efficacy in reducing brief exposure to a carbapenem can increase the risk of
TRUBP-associated infections;103 despite a higher rate subsequent colonization with carbapenem-resistant bac-
of bacteriuria reported with fosfomycin trometamol teria.110 In areas with a low prevalence of antimicrobial
than with fluoroquinolones (8.6% versus 4.2%), less resistance, use of these agents should be limited to the
resistance was reported for fosfomycin trometamol treatment of serious TRUBP-related infections.85
guidelines for the treatment of uncomplicated UTI and evaluation of a subgroup of studies in which more than
pyelonephritis in women in 2010.125 Increasing antibi- 20% of patients had urogenital abnormalities, microbio-
otic resistance in common uropathogens clearly had a logic failure occurred more frequently in patients given
significant influence on antibiotic choice for all forms of short-course therapy (≤7 days) than in those receiving
UTI. Even in the 2010 guidelines, fluoroquinolones were treatment for longer durations (>7 days).131 The evi-
no longer the first-line choice for uncomplicated UTI, dence, although hampered by small sample sizes, would
despite their effectiveness when organisms are suscep- therefore suggest that pyelonephritis in the setting of
tible to this drug class.126 Considerable regional variation urogenital abnormality should be treated for >7 days.
in resistance requires clinicians to be aware of the local
epidemiology relevant to their practice. CAUTI and health-care-associated UTI
The IDSA/ESCMID guidelines for the treatment Treatment of CAUTI with antibiotics is typically only
of uncomplicated UTI recommended the following useful in patients with appropriate symptoms or signs
four agents: nitrofurantoin, fosfomycin, pivmecillinam (for example, fever, chills, altered mental state or costo-
and trimethoprim–sulfamethoxazole. Of these, only vertebral tenderness). The presence of pyuria or malo
trimethoprim–sulfamethoxazole was recommended for dorous urine alone should not be regarded as a reason
patients with suspected early pyelonephritis. However, for treatment. A large study of bloodstream infection
avoidance of trimethoprim–sulfamethoxazole was rec- in catheterized individuals showed that 34% of patients
ommended if local resistance patterns were known to did not receive appropriate empirical therapy within the
exceed 20% or if this antibiotic combination had been first 24 h after the blood cultures were collected.132 In this
administered to the patient in the previous 3 months.125 study, although E. coli was the most common organism
A more recent review of 27 randomized controlled trials, isolated (accounting for 40% of bloodstream infections),
six systematic reviews and 11 observational studies have P. aeruginosa accounted for 21% of cases and enterococci
broadly supported these recommendations.126 In addition were responsible for 8% of cases.132 Empirical therapy
to concerns about resistance, β‑lactam antibiotics such comprising piperacillin–tazobactam or ampicillin plus
as amoxicillin–clavulanate and cefpodoxime are not as gentamicin might therefore be appropriate in many cir-
effective as other first-line choices. cumstances, until therapy can be targeted according to
For women with acute uncomplicated cystitis, typical antibiotic susceptibilities. However, such decisions should
treatment durations range from a single dose for fosfo- always be directed by knowledge of local resistance
mycin, 3 days for trimethoprim–sulfamethoxazole or rates—high prevalence of aminoglycoside resistance in
fluoroquinolones, and 5 days for nitrofurantoin. Expert Enterobacteriaceae or P. aeruginosa, for instance, will
recommendations127 have suggested that men should influence the choice of empirical therapy.
receive a longer duration of antibiotic therapy (7–14 days), As is the case with CAUTI, antibiotic overuse is
although the evidence base for this approach is not strong. common in the context of hospital-acquired, non-
A randomized controlled trial of 3 days versus 14 days of catheter-associated UTI. In the vast majority of circum
ciprofloxacin for UTI in patients with spinal cord injury stances, asymptomatic bacteriuria does not require
(70% of whom were male) suggested improved clinical antibiotic therapy. In an innovative approach to this
and microbiological outcome with 14 days of therapy.128 problem, Leis and colleagues 133 suppressed results
However, in a large observational study of UTI in men, a on urine cultures from hospitalized, noncatheterized
treatment duration of >7 days had no additional benefit patients, instead asking clinicians to call the micro
over shorter-duration treatment, but carried an increased biology laboratory for the results if UTI was suspected.
risk of Clostridium difficile infection.129 They found that 89% of urine samples from hospitalized
For the treatment of pyelonephritis in areas where resis- patients failed to meet the CDC criteria for UTI and,
tance of E. coli to fluoroquinolones exceeds 10%, an initial compared with baseline, suppression of results dropped
single dose of ceftriaxone or an aminoglycoside is recom- initiation of antibiotic therapy from 48% of occasions to
mended.125 If the patient does not require hospitalization, just 12%, with no untoward effects.133
treatment choices include a fluoroquinolone for 7 days
or trimethoprim–sulfamethoxazole for 14 days, depend- UTI caused by MDR Gram-negative bacteria
ing on the local pattern of resistance.125 Nitrofurantoin, Treatment guidelines are frequently insufficient when
fosfomycin or pivmecillinam should be avoided for the infections with MDR uropathogens are encountered.
treatment of pyelonephritis owing to concerns that they Assuming that antibiotic treatment is required (that is,
do not achieve adequate levels in renal tissue.125 The culture results do not reflect asymptomatic colonization)
treatment duration of 7 days for a fluoroquinolone for and that infectious source control has been addressed
the treatment of pyelonephritis was established follow- adequately, options are often limited. Empirical antibiotic
ing the results of a large randomized, controlled trial in choice is determined by risk factors for MDR pathogens
Sweden in which 7 days of therapy was found to be just specific to local circumstances, prior antibiotic exposure
as effective as 14 days,130 and has been confirmed by a and the severity of illness (Table 2). Consultation with
meta-analysis of eight randomized, controlled trials.131 an infectious disease practitioner is recommended for
However, only three of the randomized, controlled trials complicated UTI with MDR or XDR pathogens as addi-
evaluated antibiotics other than fluoroquinolones, which tional susceptibility testing, use of uncommon agents or
is noteworthy given current rates of resistance. In an use of combination therapy is often required. Interest
Table 2 | Treatment options for MDR Gram-negative uropathogens will be available for clinical use (Table 3). The novel
β‑lactamase inhibitor avibactam has activity against
Indication First-choice antibiotic therapy Second-choice antibiotic therapy
ESBLs, AmpC, KPC-type and some OXA-type enzymes
Uncomplicated Fosfomycin, nitrofurantoin, Quinolone, temocillin (if available), (but not metallo‑β-lactamases such as NDM-type) and
lower UTI with pivmecillinam nitroxoline (if available)
MDR organisms has been developed to be given in combination with
established antibiotics such as ceftazidime.136,137 The
Complicated Carbapenems (for example, Combination therapy (including
UTI with MDR against ESBL or XDR isolates) combination of ceftazidime and avibactam has com-
infection AmpC‑producers) Colistin* plus carbapenem‡ pleted phase III trials (including complicated UTI
(specialist Piperacillin–tazobactam might Colistin* plus aminoglycoside and abdominal infections)137,138 and was granted FDA
advice required) be an alternative against (for example, amikacin) approval in February 2015 for the treatment of compli-
ESBL-producers that are Carbapenem‡ plus aminoglycoside
susceptible (but their role Dual carbapenems cated UTI and intra-abdominal infections. Ceftolozane is
is controversial) a novel cephalosporin that has enhanced activity against
*Polymyxin B is an alternative to colistin but is less widely available. ‡Consider extended or continuous P. aeruginosa.134 It can be inactivated by β‑lactamases so
infusions of carbapenems to optimize pharmacokinetic and pharmacodynamic parameters. is formulated with tazobactam, a β‑lactamase inhibitor.
Abbreviations: MDR, multidrug resistant; UTI, urinary tract infection; XDR, extensively drug resistant.
This combination shows good in vitro activity against
a range of Gram-negative organisms, including MDR
Table 3 | Future treatment options for MDR Gram-negative uropathogens and XDR strains,139 and was granted FDA approval for
the treatment of complicated UTI and intra-abdominal
Drug Phase I* Phase II* Phase III* FDA approved
infections in December 2014. Novel aminoglycosides,140
β-lactam combined with β‑lactamase inhibitors quinolones,141 tetracyclines142 and carbapenems143,144 are
Ceftazidime–avibactam138 also in development for the treatment of UTI caused by
Ceftolozane–tazobactam139 MDR Gram-negative bacteria (Table 3). Temocillin, a
previously overlooked agent (owing to its lack of activ-
Ceftaroline fosamil–avibactam – –
ity against Gram-positive bacteria, P. aeruginosa and
Carbapenems anaerobes145), is being re-evaluated and has performed
Panipenem–betamipron143‡ – well in the treatment of urinary and bloodstream infec-
Biapenem 144§
– – tions caused by ESBL-producing and AmpC-producing
Quinolones
Enterobacteriaceae.146 Nitroxoline is an older antibiotic
that has broad-spectrum activity against many uropatho-
Finafloxacin141 – –
gens (except P. aeruginosa) and is being re-evaluated as
Aminoglycosides an option for treatment of uncomplicated UTI, although
Plazomicin140 – – it is not widely available outside Europe.147
Tetracyclines An important measure to help prevent MDR infections
is the effective implementation of antimicrobial steward-
Eravacycline142 – –
ship (a systematic approach within health-care services to
β-lactamase inhibitors combined with carbapenem optimize the use of antimicrobial agents).148 The uncon-
Relebactam (MK‑7655)– – trolled use of broad-spectrum antibiotics leads to high
imipenem cilastatin rates of resistance, especially in countries with less-robust
RPX7009–meropenem – – health-care systems.15
Other agents in development
S‑649266 – –
Novel approaches to recurrent UTI
(novel cephalosporin) Recurrent UTI is defined as the occurrence of three
GSK2251052 (Boron- – –
symptomatic UTIs within 12 months or two symptomatic
containing agent targeting episodes occurring within 6 months following clinical
Leucyl tRNA synthetase) resolution of a previous UTI. A novel method has been
*Clinical trial phases according to ClinicalTrials.gov. ‡Only approved in China, Korea and Japan. developed for the management of recurrent UTI, based on
Only approved in Japan.
the concept of bacterial interference whereby bacteria of
§
Several clinical trials have been designed in order to Responding to the challenges of Gram-negative resis-
assess whether deliberate colonization of the urinary tance will require a multifaceted approach including
tract with E. coli 83972 or E. coli HU2117154 (a genetically considered use of current antimicrobial agents, enhanced
modified derivative of E. coli 83972) could be of benefit in diagnostics (including rapid detection of resistance) and
preventing recurrent UTI, and thereby replace antibiotic surveillance, improved adherence to basic measures of
therapy for this purpose. Most patients whose urinary infection prevention, development of new antibiotics and
tract was successfully colonized by E. coli 83972 or E. coli research into non-antibiotic treatment and prevention
HU2117 were reported to have a significant reduction in strategies. An improved understanding of the biology
the incidence of UTI during the period of colonization. of pathogens that cause UTI will provide opportunities
Furthermore, in colonized patients who developed UTI, for the development of strategies aimed at interrupting
the infection was caused by uropathogens other than key processes such as bacterial attachment, persistence,
E. coli.152,155 Clinical trials using E. coli 83972 to pre-coat immune evasion and biofilm formation.
urinary catheters have also yielded promising results, with Human ingenuity is most productive when faced with
these trials currently being extended.156 These results high- seemingly impenetrable problems. As such, limitations
light a possible new management strategy for recurrent do exist in the concept of the “tragedy of the commons”,
UTI; however, further studies are required before bacterial which underestimates the adaptability of human societ-
interference strategies such as those using E. coli 83972 can ies and the possibility that rational self-interest might still
be adopted more widely in the clinical setting.149 generate solutions to mutual problems—especially when
The biological importance of fimbriae in UPEC colo- the status quo is in the interest of nobody.
nization of the urinary tract is confirmed by the fact that
the prevention of this adherence mechanism represents
an attractive anti-virulence strategy.157–159 Molecules that Review criteria
prevent the biogenesis or adhesion of UPEC fimbriae have Relevant articles published between January 2009 and
shown great promise in animal infection models160–162 and September 2014 were identified via PubMed using the
are also highly effective against MDR strains such as E. coli following search criteria: “urinary tract infection” AND
ST131.163 Vaccines based on the FimH and PapG adhes- “resistant OR resistance” AND “E. coli OR Klebsiella OR
ins provide protective efficacy in mouse and/or primate Proteus OR Enterobacter OR Citrobacter OR Pseudomonas
models of UTI,164,165 and together with vaccines targeted OR Acinetobacter”. Only articles published in English or
against iron-regulated surface antigens,166,167 provide a with English translation were included. Additional sources
relevant to the Review were identified by the authors
framework for the development of new prevention strat
from personal bibliographies and citations in articles
egies. However, the effectiveness of these approaches for
identified by the search strategy. In order to summarize
the prevention of UPEC-mediated UTI in different patient
prevalence data, articles reporting resistance rates in
groups requires further investigation. urinary pathogens were identified. Those reporting only
data from neonatal units, intensive care units or other
Conclusions highly selected populations were excluded. Studies
Safe urological practice relies upon the availability of effec- where denominators were not described or where data
tive antibiotics, which is now threatened by the ongoing were collected prior to 2004 were also excluded. Pooled
rapid evolution of resistance in bacterial uropathogens. estimates of resistance prevalence by country for
Although some new drugs with activity against Gram- each antibiotic class were extracted from 98 articles.
negative bacteria, including activity against strains with Where more than one study was reported for a country,
highly resistant phenotypes, might be available in the near prevalence estimates were pooled by meta-analysis using
MetaXL (EpiGear, University of Queensland, Brisbane,
future, they are likely to be expensive and might be best left
Australia) with a random effects model. Intensity maps
for situations when no alternatives exist. Furthermore, the
were generated using Tableau®(Tableau Software,
existence of a single agent with activity against the great Seattle, WA, USA).
diversity of resistance is unlikely.
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