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For efficient heat transfer, steam must flush the air out of the autoclave chamber.
If the drain screen is blocked with debris, a layer of air may form at the bottom of
the autoclave and prevent proper operation. Check the drain screen at the bottom
of the chamber before using the autoclave. Clean out any debris.
Glassware:
o Cap Pyrex bottles loosely–whether empty or filled–to prevent explosions
due to expansion.
o Cover bottles that are not made of safety glass (e.g. not Pyrex) with
aluminum foil.
Liquids: Fill containers only half full
Combination loads: Do not combine strong oxidizing material (such as dry
hypochlorites) with organic materials (such as paper, cloth or oil).
Push the "start" button on the control panel to initiate the cycle.
Each machine must have an autoclave log where the operator records the date
and other details. Fill out the log while the autoclave is "charging" or starting.