PHARMA RESEARCH & ANALYTICAL LABORATORIES

5, OLD TRUNK ROAD, PALLAVARAM, CHENNAI-600 043

QUALITY CONTROL DISPOSAL OF CULTURE AND MEDIA
QC/MCB/017/01 Revision :Original Supercedes :Nil

Effective Date : MAY-13 Review Date : APR-15 Page No. : 1 of 2

1.PURPOSE : To lay down a procedure for proper disposal of used media

plates and culture tubes.
2.SCOPE : This SOP is applicable for Media disposal.

3.RESPONSIBILITY : Microbiologist

4.ACCOUNTABILITY :QC Manager

5.PROCEDURE :
a. Wear rubber hand gloves and nose mask.

b. Sterilise the used media plates in autoclave at 15 psi, 121 °C for 30

minutes.

c. Remove media content of Petri dish with the help of spatula and
collect in a container.

d. Add 5% dettol solution to the collected waste media and dilute the
autoclaved contents with tap water and drain into the ETP plant.

e. Keep the empty glass Petri dishes for 1 hr in 5% dettol solution or

savlon solution and then wash the Petri dish with water and dry them for
reuse. Disposable plates should be discarded after keeping them for 1 hr
in 5% dettol or savlon solution

CULTURE TUBES
a.Autoclave the microbial media tubes at 15 psi, at 121 °C for
30 minutes.
b.After autoclaving add 5% dettol solution to the media and dilute the
autoclaved contents with tap water and drain into the ETPplant.

PREPARED BY CHECKED BY APPROVED BY

N.U.VIJAYALAKSHMI E.LEELAVATHY K.MANIVANNAN

Microbiologist Manager QC DGM QC
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 PHARMA RESEARCH & ANALYTICAL LABORATORIES
5, OLD TRUNK ROAD, PALLAVARAM, CHENNAI-600 043
QUALITY CONTROL DISPOSAL OF CULTURE AND MEDIA
QC/MCB/017/01 Revision :Original Supercedes :Nil

Effective Date : MAY-13 Review Date : APR-15 Page No. : 2 of 2

c. The empty tubes are dry them for reuse.

d.After completion of Autoclave cycle for discarded media/cultured
tube remove the culture tube/media from the autoclave and start a
blank autoclave cycle without material to eliminate the possibility of
any contamination.
e. Media preparation and media decontamination (used media discard)
activities shall not be done simultaneously.
6.FERQUENCY : Daily
7.END OF THE DOCUMENT

PREPARED BY CHECKED BY APPROVED BY

N.U.VIJAYALAKSHMI E.LEELAVATHY K.MANIVANNAN

Microbiologist Manager QC DGM QC
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