Thyroid Panel MLS4C PDF

THYROID PANEL
CLINICAL CHEMISTRY 3 LABORATORY | MLS4C

THYROID PANEL
THYROID PANEL
THYROID STIMULATING HORMONE

FREE T3/FREE TRIIODOTHYRONINE
FREE T4/FREE THYROXINE
T3/TRIIODOTHYRONINE
T4/THYROXINE
THYROID PANEL
Mini VIDAS BLUE OPERATION

HARDWARE
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Mini VIDAS BLUE OPERATION: HARDWARE

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SECTION:
• Reagent Strip Tray
• SPR Block
• Operating Status Light
• Microprocessor
• Incubators
• Strip Preparation Tray
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SECTION:
• SPR Block
• Microprocessor
• Incubators
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SECTION:
• SPR Block
• Microprocessor
• Incubators
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Strip
Preparation
Tray
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Detection
System:
Fluorometric
Scanner
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Central Processing Unit

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Keypad and Screen

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Display Screen
Option
Selection Keys
Function Keys
Arrow Keys
Numeric
Keypad
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Internal Printer
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Reference:
1. Memory Card Slot
2. Diagnostic Port
3. Computer Port
4. Printer Port
5. AC Power Connection
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Memory Card Slot
Diagnostic Port
Computer Port
Printer Port
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External Bar Code Reader Power

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Two Bar Code Readers:

1. Hand-Held
2. Inside the Instrument
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SOFTWARE
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Mini VIDAS BLUE OPERATION: SOFTWARE

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Selection
Selection Status
Labels
The "•–“ Display

Marker Temperature
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Selection
Selection Status
Labels
The "•–“ Display

Marker Temperature
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Screen Label
Standard,
Position #
Control
Start/Halt
Assay Code
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Screen Label
SPR Column
Reagent Strip
Tray Column
Sections
Print
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Screen Paging
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Paging Bar
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THE ASSAY KIT
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Mini VIDAS BLUE OPERATION: THE ASSAY KIT

8
1. Bar Code 5. Batch Code

2. Alphanumeric Representation of 6. Expiration Date
the Bar Code 7. Sequence Number
3. Assay 8. Sample Well
4. ID/Identification 9. Optical Cuvette
THYROID PANEL
Mini VIDAS BLUE OPERATION: THE ASSAY KIT

• The SPR block is part of an automated pipetting
system that uses the SPRs to mix and transfer
reagents during processing.
• sampling,
• incubation,
• mixing,
• washing.
THYROID PANEL
Mini VIDAS BLUE OPERATION:

SAMPLE & TEST INPUT
THYROID PANEL
Mini VIDAS BLUE OPERATION: SAMPLE & TEST INPUT

How to know if a Calibration is valid?
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How to Read a MLE Card?
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RUN STANDARDS (S) AND CONTROLS (C) FOR CALIBRATION
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RUN STANDARDS (S) AND CONTROLS (C) FOR CALIBRATION & SAMPLE ID
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SAMPLE ID
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RUN SAMPLE
Place the strip (s) Dispense the Sample

Leave reagents 30 min at room Check the type of
and SPR (s) into volume into the sample
temperature before use sample
the section well of the strip
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RUN SAMPLE
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RUN SAMPLE
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RUN SAMPLE
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RUN SAMPLE
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RUN SAMPLE
• Reaction starts: the green LED of
the section lights up
• Reaction ends: the green LED
flashes
• Remove the strip(s) and SPR(s) of
the section
• Results print out automatically
• Interpretation and measurement
range
THYROID PANEL
Mini VIDAS BLUE OPERATION: MAINTENANCE
Strip Preparation Tray Reagent Strip Tray
Clean for every 6 months

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Mini VIDAS BLUE OPERATION: MAINTENANCE
Optical Lenses SPR Block
Clean for every month

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TROUBLESHOOTING
• The mini VIDAS® analyzer has several
ways to indicate errors: an alarm (a
beep) and an optional visual cue (the
display blinks).
THYROID PANEL

TROUBLESHOOTING
Errorcode: 73
Time: 14:53:20
14/09/15
MLE Card data bad
Start Error Other Error Messages

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THYROID PANEL

• The immunoenzymatic determination
of thyroid-stimulating hormone in
human serum or plasma (lithium
heparin) using the ELFA technique
(Enzyme Linked Fluorescent Assay).
THYROID PANEL

Solid Phase Receptacle
• The interior of the SPR is coated
during production with anti-TSH
immunoglobulins (mouse)
THYROID PANEL

STRIP - WELL
• The well of the strip is contained with
anti-TSH antibody labeled with alkaline
phosphatase
THYROID PANEL

PRINCIPLE
The well is contained with anti-TSH

antibody labeled with alkaline
phosphatase (conjugate)
THYROID PANEL

PRINCIPLE
Add sample (lithium heparin)

containing the Thyroid Stimulating
Hormone
THYROID PANEL

PRINCIPLE
Thyroid Stimulating Hormone will

bind to the exposed Fab Region of
the Anti-TSH Antibody conjugate
THYROID PANEL

PRINCIPLE
The mixture is
cycled in and out
of the SPR
THYROID PANEL

PRINCIPLE
The sensitized
Anti-TSH Antibody
will formed a
sandwich complex
with the antibody
present inside the
SPR
THYROID PANEL

PRINCIPLE
The substrate (4-

Methyl-umbelliferyl
phosphate) is cycled in
and out of the SPR
THYROID PANEL

PRINCIPLE
Catalyzes the
hydrolysis of this
substrate into a
fluorescent (4-methyl-
umbelliferone) and
measured at 450nm
THYROID PANEL
THYROID STIMULATING HORMONE: PROCEDURE
Use 1 strip and 1 SPR Mixed using a

Only remove the required
for sample, vortex-type mixer
reagents and come to RT for
control(C1), or and use 200uL
at least 30 minutes
calibrator(S1) only
The assay will be

Insert the TSH SPRs Remove the SPRs
completed within
and TSH Strips into and and strips from
approximately
the instrument the instrument
40minutes
THYROID PANEL
RANGE OF EXPECTED VALUES

Euthyroid: 0.25 - 5 μIU/ml.
Hyperthyroid: < 0.15 μIU/ml.
Hypothyroid: > 7 μIU/ml.
THYROID PANEL
T3/TRIIODOTHYRONINE
THYROID PANEL
T3/TRIIODOTHYRONINE
• the quantitative measurement of total
triiodothyronine (T3) in human serum
or plasma (lithium heparin) using the
ELFA technique (Enzyme Linked
Fluorescent Assay).
THYROID PANEL
T3/TRIIODOTHYRONINE
during production with anti-T3
antibodies (sheep)
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T3/TRIIODOTHYRONINE
STRIP - WELL
T3 antigen labeled with alkaline
THYROID PANEL
T3/TRIIODOTHYRONINE
PRINCIPLE
The well is contained with T3

Antigen labeled with alkaline
THYROID PANEL
T3/TRIIODOTHYRONINE
PRINCIPLE
Add sample (lithium heparinized

serum or plasma) containing the T3
THYROID PANEL
T3/TRIIODOTHYRONINE
PRINCIPLE
T3 will be mixed with the T3

Antigen labeled with Alkaline
Phosphatase
THYROID PANEL
T3/TRIIODOTHYRONINE
PRINCIPLE
The mixture is
cycled in and out
of the SPR
THYROID PANEL
T3/TRIIODOTHYRONINE
PRINCIPLE The mixture of T3
antigen and T3 Antigen
labeled with Alkaline
Phosphatase will
compete with the
limited binding site of
the anti-T3 antibodies
(sheep) present
THYROID PANEL
T3/TRIIODOTHYRONINE
PRINCIPLE
The substrate (4-

Methyl-umbelliferyl
and out of the SPR
THYROID PANEL
T3/TRIIODOTHYRONINE
PRINCIPLE
Catalyzes the
hydrolysis of this
substrate into a
umbelliferone) and
measured at 450nm
THYROID PANEL
T3/TRIIODOTHYRONINE: PROCEDURE

at least 30 minutes
calibrator(S1) only
The assay will be

Insert the T3 SPRs and Remove the SPRs
completed within
T3 Strips into the and and strips from
approximately
instrument the instrument
40minutes
THYROID PANEL
T3/TRIIODOTHYRONINE

Euthyroid: 0.92 to 2.33nmol/L
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T4/THYROXINE
THYROID PANEL
T3/TRIIODOTHYRONINE
• the determination of total thyroxine
(T4) in human serum and plasma
(lithium heparin) using the ELFA
technique (Enzyme Linked Fluorescent
Assay).
THYROID PANEL
T4/THYROXINE
during production with anti-T4
antibodies
THYROID PANEL
T4/THYROXINE
STRIP - WELL
T4 antigen labeled with alkaline
THYROID PANEL
T4/THYROXINE
PRINCIPLE
The well is contained with T4

Antigen labeled with alkaline
THYROID PANEL
T4/THYROXINE
PRINCIPLE
Add sample (lithium heparinized

serum or plasma) containing the T4
Antigen
THYROID PANEL
T4/THYROXINE
PRINCIPLE
T4 will be mixed with the T4

Antigen labeled with Alkaline
Phosphatase
THYROID PANEL
T4/THYROXINE
PRINCIPLE
The mixture is
cycled in and out
of the SPR
THYROID PANEL
T4/THYROXINE
PRINCIPLE The mixture of T4
antigen and T4 Antigen
labeled with Alkaline
Phosphatase will
compete with the
limited binding site of
the anti-T43
antibodies present
THYROID PANEL
T4/THYROXINE
PRINCIPLE
The substrate (4-

Methyl-umbelliferyl
and out of the SPR
THYROID PANEL
T4/THYROXINE
PRINCIPLE
Catalyzes the
hydrolysis of this
substrate into a
umbelliferone) and
measured at 450nm
THYROID PANEL
T4/THYROXINE: PROCEDURE

at least 30 minutes
calibrator(S1) only
The assay will be

Insert the T4 SPRs and Remove the SPRs
completed within
T4 Strips into the and and strips from
approximately
instrument the instrument
40minutes
THYROID PANEL
T4/THYROXINE

Euthyroid: 60 to 120nmol/L
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TSH/T3/T4
CONSIDERATION, LIMITATION &
STORAGE
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TSH/T3/T4
LIMITATIONS OF THE METHOD
• Interference may be encountered
with certain sera containing
antibodies directed against reagent
components.
• Must be interpreted as part of a
complete clinical profile
THYROID PANEL
TSH/T3/T4
STORAGE CONSIDERATION
• Storage Temperature @ 2 to 8°C
• Do not freeze the SPRs and Strips
• Check for correct sealed and
undamaged SPR pouches
THYROID PANEL
TSH/T3/T4
SAMPLE CONSIDERATIONS
Hemolysis
Lipemia
2 to 8°C for 48 hours Bilirubinemia
-25+-6°C for 2 months
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TSH/T3/T4
CALIBRATION
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THYROID PANEL SUMMARY

WELL
SAMPLE TESTING MEASUREMENT INTENTSITY &
PARAMETERS (with SPR SPECIMEN
VOLUME TIME RANGE CONC.
ALP)
anti-TSH Serum,
Thyroid
Anti-TSH Immuno Plasma
Stimulating 200uL 40 min 0.05 to 60uIU/mL Proportional
Antibody globulins (Hep.,
Hormone
(mouse) Beads, Gel)
anti-T3 Heparinized
T3 T3 Inversely
antibodi Serum or 100uL 40 min 0.4 to 9nmol/L
Triiodothyronine Antigen Proportional
es Plasma
anti-T4 Heparinized
T4 T4 Inversely
antibodi Serum, 200uL 40 min 6 to 320nmol/L
Thyroxine Antigen Proportional
es Plasma
THYROID PANEL

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THYROID PANEL

CLINICAL CHEMISTRY 3 LABORATORY | MLS4C

THYROID STIMULATING HORMONE

Mini VIDAS BLUE OPERATION

Mini VIDAS BLUE OPERATION: HARDWARE

Mini VIDAS BLUE OPERATION: HARDWARE

Mini VIDAS BLUE OPERATION: HARDWARE

Mini VIDAS BLUE OPERATION: HARDWARE

Mini VIDAS BLUE OPERATION: HARDWARE

Mini VIDAS BLUE OPERATION: HARDWARE

Mini VIDAS BLUE OPERATION: HARDWARE

Central Processing Unit

Mini VIDAS BLUE OPERATION: HARDWARE

Keypad and Screen

Mini VIDAS BLUE OPERATION: HARDWARE

Mini VIDAS BLUE OPERATION: HARDWARE

Mini VIDAS BLUE OPERATION: HARDWARE

Mini VIDAS BLUE OPERATION: HARDWARE

Mini VIDAS BLUE OPERATION: HARDWARE

Mini VIDAS BLUE OPERATION: HARDWARE

Memory Card Slot

Mini VIDAS BLUE OPERATION: HARDWARE

External Bar Code Reader Power

Mini VIDAS BLUE OPERATION: HARDWARE

Mini VIDAS BLUE OPERATION: HARDWARE

Two Bar Code Readers:

Mini VIDAS BLUE OPERATION

Mini VIDAS BLUE OPERATION: SOFTWARE

Mini VIDAS BLUE OPERATION: SOFTWARE

Mini VIDAS BLUE OPERATION: SOFTWARE

Mini VIDAS BLUE OPERATION: SOFTWARE

The "•–“ Display

Mini VIDAS BLUE OPERATION: SOFTWARE

The "•–“ Display

Mini VIDAS BLUE OPERATION: SOFTWARE

Mini VIDAS BLUE OPERATION: SOFTWARE

Mini VIDAS BLUE OPERATION: SOFTWARE

Mini VIDAS BLUE OPERATION: SOFTWARE

Mini VIDAS BLUE OPERATION: SOFTWARE

Mini VIDAS BLUE OPERATION

Mini VIDAS BLUE OPERATION: THE ASSAY KIT

1. Bar Code 5. Batch Code

Mini VIDAS BLUE OPERATION: THE ASSAY KIT

Mini VIDAS BLUE OPERATION:

Mini VIDAS BLUE OPERATION: SAMPLE & TEST INPUT

Mini VIDAS BLUE OPERATION: SAMPLE & TEST INPUT

Mini VIDAS BLUE OPERATION: SAMPLE & TEST INPUT

Mini VIDAS BLUE OPERATION: SAMPLE & TEST INPUT

Mini VIDAS BLUE OPERATION: SAMPLE & TEST INPUT

Mini VIDAS BLUE OPERATION: SAMPLE & TEST INPUT

Mini VIDAS BLUE OPERATION: SAMPLE & TEST INPUT

Mini VIDAS BLUE OPERATION: SAMPLE & TEST INPUT

Mini VIDAS BLUE OPERATION: SAMPLE & TEST INPUT

Mini VIDAS BLUE OPERATION: SAMPLE & TEST INPUT

Mini VIDAS BLUE OPERATION: SAMPLE & TEST INPUT

Mini VIDAS BLUE OPERATION: SAMPLE & TEST INPUT

Mini VIDAS BLUE OPERATION: SAMPLE & TEST INPUT

Mini VIDAS BLUE OPERATION: SAMPLE & TEST INPUT

Mini VIDAS BLUE OPERATION: SAMPLE & TEST INPUT

Mini VIDAS BLUE OPERATION: SAMPLE & TEST INPUT

Mini VIDAS BLUE OPERATION: SAMPLE & TEST INPUT

Mini VIDAS BLUE OPERATION: SAMPLE & TEST INPUT

Mini VIDAS BLUE OPERATION: SAMPLE & TEST INPUT