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Label free quantification is a method in mass spectrometry that aims to determine the
relative amount of proteins in two or more biological samples.
Initial commercial isobaric tag offerings isobaric tags for relative absolute
quantitation (iTRAQ) in 4-plex and 8-plex configurations and tandem mass tags
(TMT) in a 6-plex configuration. Recently, TMT reagens were expanded to 10-plex
configuration, by the addition of mass defect-based variants , for use with
high-resolution MS platforms. These products have established themselves as
powerful tools for high-throughput quantitative proteomics studies.
iTRAQ has been uitilized to identify biomakers for various disease condition. It has
been suggested to be suitable for the discovery of biomakers in a wide range of body
fluids and tissues, including serum and plasma.
The quantitative information of the proteome can help us understand the biological
function of proteins in the interaction network. Quantitative proteomics has been
widely used in screening biomarkers in various cancers and is becoming an important
tool for differential and dynamic proteomics research. With the determination of the
absolute quantitation of the biomarker, we can directly judge the status of a disease,
which will benefit the clinical diagnosis and the treatment of the disease.