Curriculum Vitae

Dr. Basir Ahmad

Dipartimento di Scienze Biochimiche,Università di Firenze,Viale Morgagni 50,
50134, Florence, Italy Tel. +39-055-4598319, Mobile. +39-3270415667
E.mail: ahmad.basir1@gmail.com, ahmad.basir@unifi.it

Education
December 2007 Postdoctoral Fellow of EURAMY - Systemic Amyloidoses
-present in Europe
Group: Prof. Fabrizio Chiti, Dipartimento di Scienze
Biochimiche, Università di Firenze

April 2008 PhD (Biotechnology)

Interdisciplinary Biotechnology Unit, AMU, India
Thesis Title: "Studies on the folding intermediates of
multidomain and multimeric proteins”

June 2002 Master of Science (Biotechnology)

Interdisciplinary Biotechnology Unit, AMU, India
Subjects Studied: Molecular Biology, Biochemistry,
Enzymology, Biochemical Engineering, Chemistry,
Immunology, Microbiology, Biophysical Chemistry
Thesis Title: Salt induced refolding in different domains of
partiallyfolded bovine serum albumin:

July 1999 Bachelor of Science (Chemistry Honors)

Department of Chemistry AMU, India

Research Interest: Protein misfolding/Amyloid aggregation, Amyloid fibrils as

nanomaterial, Protein folding/stability, Protein design, Protein
ligand interaction
Publications
1. Ahmad B, Khan MK, Haq SK and Khan RH. (2004) Intermediate formation at
lower urea concentration in ‘B’ isomer of human serum albumin: a case study
using domain specific ligands. Biochem. Biophys. Res. Commun. 314: 166-173.
2. Ahmad B, Kmal MZ and Khan RH. (2004) Alkali-induced conformational
transitions in different domains of bovine serum albumin. Protein pept. Lett. 11:
307-315
3. Ahmad B, Ahmed MZ, Haq SK and Khan RH. (2005) Guanidine hydrochloride
denaturation of human serum albumin originates by local unfolding of some
stable loops in domain III. Biochim. Biophys. Acta. 1750: 93-102.
4. Ahmad B, Ankita and Khan RH. (2005) Urea induced unfolding of F isomers of
human serum albumin: a case study using multiple probes. Arch. Biochem.
Biophys. 437: 159-167
5. Ahmad B, Ansari MA, Sen P and Khan RH. (2006) Low versus high molecular
weight poly(ethylene glycol) induced states of stem bromelain at low pH:
stabilization of molten globule and unfolded states. Biopolymers 81: 350-359.
6. Ahmad B, Parveen S. Khan RH. (2006) Effect of Albumin conformation on the
binding of ciprofloxacin to human serum albumin: a novel approach directly
assigning binding site. Biomacromolecules. 7: 1350-1356.
7. Ahmad B, Khan RH, (2006) Studies on the acid unfolded molten globule state of
catalatically active stem bromelain: A comparison with catalytically inactive
form. J. Biochem (Tokyo) 140: 501-508
8. Ahmad B., Shamim, T.A., Haq, S.K. and Khan R.H. (2007) Identification and
characterization of functional intermediates of stem bromelain during urea and
guanidine hydrochloride unfolding J. Biochem (Tokyo) 141: 251-259.
9. Tayyab S, Ahmad B, Kumar Y and Khan MM (2002) Salt induced refolding in
different domains of partially folded bovine serum albumin. Int. J. Biol.
Macromol. 30: 17-22.
10. Naseem F, Ahmad B, Ashraf MT and Khan RH. (2004) Molten globule like
folding intermediate of asialofetuin at acidic pH. Biochim. Biophys. Acta. 1699:
191-199.
11. Fatima S, Ahmad, B and Khan RH. (2006) Fluoroalcohols induced unfolding of
succinylated ConA: native like beta structure in partially folded intermediate and
alpha-helix in molten globule state. Arch. Biochem. Biophys. 454: 170-180.
12. Haq SK, Rasheedi S, Sharma P, Ahmad B and Khan RH. (2005) Influence of salt
and alcohols on the conformation of partially folded intermediate of stem
bromelain at low pH. Int. J. Biochem. Cell Biol. 37: 361-374.
13. Younus H, Jamal S, Ahmad B and Saleemuddin M. (2006) Investigation of
conformational changes induced by binding of pancreatic Rnase to anti-Rnase
IgG derived Fab monomer using optical procedures. Biochemistry (Mosc) 71:
218-221.
14. Gull N, Kumar S and Ahmad B, Khan RH and Kabir–ud-Din (2006) Influence of
urea additives on micellar morphology / protein conformation, Colloids and
Surfaces B. 51: 10-15.
15. Hameed M, Ahmad B, Fazili KM, Andrabi K, Khan RH. (2007) Different Molten
Globule-like Folding Intermediates of Hen Egg White Lysozyme Induced by
High pH and Tertiary Butanol., J Biochem (Tokyo). 141(4):573-83.
16. Fatima S, Ahmad B and Khan RH. (2007) Native-like tertiary structure in the
Mucor miehei lipase molten globule state obtained at low pH.
IUBMB Life. 59(3):179-86.
17. Sen P, Ahmad B, Khan RH. (2008) Formation of a molten globule like state in
bovine serum albumin at alkaline pH Eur Biophys J (in press)
18. Varshney A, Ahmad B, Khan RH. (2008) Comparative studies of unfolding and
binding of ligands to human serum albumin in the presence of fatty acid:
Spectroscopic approach Int. J. Biol. Macromol. (in press)

Academic honors/ Awards/ Scholarship

DBT Studentship: Awarded from Department of Biotechnology, Govt. of India
(1999-2001)
Qualified CSIR-UGC National Eligibility Test (NET) for Lectureship, 2002
Junior Research Fellowship: Awarded from the Council of Scientific and Industrial
Research, Govt. of India (2003-2005)
Senior Research Fellowship: Awarded from the Council of Scientific and Industrial
Research, Govt. of India (2005- 2007)
EURAMY -Postdoctoral Fellowshiop: Awarded from European Union, Istituto
Nazionale Biostrutture E Biosistemi (INBB), Rome,
Italy
Technical Experience
Molecular Biology: Site directed mutagenesis, Plasmid and Chromosomal DNA
isolation, PCR, Gene Expression
Protein Chemistry:
Protein Expression & Purification (Ion exchange, Gel filtration, hydrophobic and
affinity chromatography, PAGE native and SDS
Spectroscopic techniques:
Circular dichroism (CD), Fluorescence, UV/Visible, Dynamic Light Scattering (DLS),
FTIR
Data Analysis: Protein structure-function relationship, Protein unfolding/refolding,
stability study, Aggregation and amyloid fibrils studies Thermodynamic data, Ligand-
protein interaction data. Enzyme kinetics data.
Others: ELISA, Lectin isolation, Lectin-sugar interaction.
Conference / Symposia attended
Attended and Presented posters In
1. The Second Indian Symposium of the Protein Society: Protein Structure and Function
held at Indian Institute of Science Bombay INDIA. October 28-30, 2004
2. International Symposium on Molecules, Machines and Networks held at NCBS,
Bangalore INDIA. January, 6-9, 2004

3. National Symposium on Stability and Stabilization of Biomacromolecules held at

Interdisciplinary Biotechnology Unit, AMU, Aligarh INDIA. 2002

4. Workshop on ‘Bioinformatics in the study of biomacromolecules organized by the

distributed information sub-centre, AMU, Aliharh. February, 8-10, 2005
General Skills
Languages English, Hindi, Urdu
Scientific Packages Origin, SigmaPlot, AGADIR, Aggrescane,
FoldX
Oprating system Microsoft Window
References
1. Prof. Kabir-ud-Din
Faculty of Science, Department of Chemistry,
Aligarh Muslim University, Aligarh 202 002, India
Email: kabir7@rediffmail.com
2. Dr Khan RH (phd supervisor)
Interdisciplinary Biotechnology Unit,
AMU, Aligarh-202002 India
Email: rizwanhkhan@hotmail.com
3. Profesor Saad Tayyab
Institute Of Biological Sciences,
Faculty Of Science, University Malaya,
50603 Kuala Lumpur
Email: saadtayyab2004@um.edu.my
4. Prof Fabrizio Chiti
Dipartimento di Scienze Biochimiche
Università di Firenze,Viale Morgagni 50, 50134
Florence, Italy
Email: chitif@unifi.it