How to optimize your

ICP-OES methods
E-book
How to optimize your
ICP-OES methods
E-book

With the increasing sophistication of instrument software, it can be difficult to keep up with all
the powerful functionality that’s built in–functionality that could be making your life easier.
Here are the top 9 functions you should know about.

Moreover, find in this ebook how the ICP Expert Software features an array of flexible
performance and productivity enhancements that add more power to your ICP-OES analyses.

Now you can get the right results every time and :

- Remove the guesswork from you method development

- Benefit from the advanced automation features to boost productivity
- Maximize instrument uptime with smart diagnostics

Discover how with the 9 Tips to optimize your ICP-OES methods and a selection of
ICP Expert technical notes.
How to optimize your
ICP-OES methods
9 tips to make you more efficient
(and get the right results)
9 tips to make you more efficient
(and get the right results)

With the increasing sophistication of instrument software, it can be difficult to keep

up with all the powerful functionality that’s built in–functionality that could be making
your life easier.
Here are the top 9 functions you should know about. Most of them are available on all
manufacturers’ current ICP-OES instruments.

Setting the rinse time in a method can be tricky. If the sample rinse time
in your method is too short, you risk introducing carryover. Residual
Use dynamic rinse
liquid from the previous sample will impact the accuracy and precision
1 times to slash
sample-to-sample
of the next sample. Conversely, if the sample rinse time is too long,
then you are wasting time and rinse solution.
run time
Use a dynamic rinse control function that automatically adjusts the rinse time
to suit the concentration of each sample. Typically, these functions monitor
the signal to detect when all the sample has been rinsed out. When it reaches
a user-defined threshold, the instrument moves to the next sample.

Labs often run only a limited number of methods on each ICP-OES. You

2 Create shortcuts for

common methods
can save time when switching between methods by using functionality that
creates shortcuts to those methods. Analysts can then load a method and be
ready for analysis with just one click.

Most ICP-OES instruments can be fitted with a switching valve positioned

Install a switching between the peristaltic pump and the spray chamber. This multiport valve

3 valve to run more allows sample to be loaded while the rinse solution is pumped into the sample
samples with higher introduction system. Using a switching valve eliminates delays between rinsing
accuracy and sample measurement, saving time and gas and improving precision, and
stability. There are even models with extra ports to enable online addition of
internal standards. A fully integrated switching valve is the best choice as it
can then be controlled within the instrument method.
9 tips to make you more efficient
(and get the right results)

If you have multiple analysts using the same ICP-OES, consistent torch
alignment may be a problem. The process of removing a torch and getting

4 Reproduce the torch

position—each time
the replacement one back in the same position can be tricky. With torch
alignment having a big impact on instrument performance, it’s important to
get it right. Most instruments have an auto-alignment feature that makes torch
positioning easy and reliable. Some even have a plug-and-play torch design
that eliminates the need for alignment completely.

If you are receiving samples from new customers, or new sources, they
may contain unexpected interferences, which can cause major headaches.

5 Identify unexpected
interferences before
Identifying and correcting for interferences is critical for ensuring accuracy—
but it often requires the skills of an experienced spectroscopist. Look for
they impact results
functionality in your instrument software that manages interferences by
dynamically adjusting background correction points. Typically, this is based on
a spectrum of the sample, which highlights any potential interferences.

Figuring out what went wrong when you get an inaccurate analytical result
can be challenging. Fortunately, there are software functions available that
Troubleshoot

6
take a snapshot of each sample scan during the analysis. You can quickly
erroneous results
review a sample scan to see what might have caused the error—the problem
without re-analysis
might be interference from another element or elements at unexpectedly
high concentrations. Sometimes, you can reprocess the data from that sample
scan to deliver a correct result, without rerunning the sample.
9 tips to make you more efficient
(and get the right results)

Most instruments allow you to choose an extra wavelength to the primary

Use a secondary emission line for each element you’ve selected for analysis. This function is a

7 wavelength to lifesaver when you have high-matrix samples with substantial interferences,
confirm sample as it gives you confidence that you can use data from another wavelength
results if things don’t work out with the primary one. Collecting data from multiple
wavelengths per element is also useful for routine samples. You can use the
data from each line to compare the reported concentration of the element. If
the concentrations match up, you’re assured that the result is correct.

Occasionally a run has been going for some time when you notice a decrease
in sensitivity. Many instruments have built-in diagnostic tests to help identify
8 Use the instrument
test tools what might be going wrong. Simply stop the run, run the tests, and use the
results to guide you in troubleshooting the problem.

Hitting the top of your calibration range can force you to dilute and rerun samples.
There’s functionality available that allows you to monitor two or more
wavelengths of different sensitivities for each element. If you suffer an

9 Increase the dynamic

range
overrange result using the most sensitive wavelength, the instrument will
automatically measure using the less sensitive wavelength, ensuring all
your results stay within the calibration range. Often, this process can be
completely automated.

This information is subject to change without notice.

© Agilent Technologies, Inc. 2017

Published in the USA, December 1, 2017
5991-8687EN
ICP Expert software

Technical Overview

Introduction

The Agilent 5110 ICP-OES provides fast sample analysis, using less gas,
without compromising on performance for tough samples. It has been
designed for ease-of-use, with this philosophy driving the design of both the
hardware and the ICP Expert software that controls the instrument.

With a familiar worksheet interface, simplified method development with

IntelliQuant mode, expanded QC functionality, an instrument dashboard, and
software applets that automatically load pre-set method templates, the ICP
Expert software significantly simplifies analysis.
Easy-to-use

Agilent ICP Expert software uses application-specific

software applets that allow users of all skill levels to
confidently run ICP-OES analyses.

The administrator can choose which application

methods are available to each user. This is done via the
ICP Applet screen (Figure 2). The novice user can then
simply walk up and click the ICP applet icon (Figure
1, step 1). The applet automatically loads the method
(Figure 1, step 2) so they can immediately start analysis
by igniting the plasma and then clicking the ‘Run’
button (Figure 1, step 3).

Prompts are displayed to guide the user, informing them

when to introduce each standard and when to present
the appropriate sample for measurement, according to
the sequence order. This step-by-step approach ensures
that all users can achieve accurate and reproducible
results with minimal training. Applets can easily be
imported and exchanged with other users by using the
Applet Management tool.
Figure 2. The interface can be tailored so that each user sees only those
features they need. This reduces complexity.
Simplified method development
The Agilent ICP Expert software makes method
development simple, so you can spend less time setting
up methods, and more time running samples.

Select the elements to be determined and the

software displays the emission lines ranked in order of
preference. Select an emission line wavelength from
the list and the potential interferences are graphically
displayed, with the other elements that have been
selected for analysis highlighted in red. You can then
visually assess the potential for interferences from
the other analytes. If there are lines that are too close
together, you can zoom in for a closer look. You can then
select a wavelength that avoids spectral interferences
and covers the required concentration range for your
sample types (Figure 3).

Figure 3. View potential interferences at a glance during method

Figure 1. Application-specific software applets guide the user through the development. Interferences are both color-coded and displayed graphically.
analysis, to ensure proper technique and accurate results.
2
Logical workflow
The ICP Expert software features a clear, logical
workflow that simplifies analyses, even for experienced
users. To set up and perform an analysis, you simply
follow the sequence of pages in the displayed order.
The current page is highlighted to provide full visibility
of your progress in the workflow (Figure 4).
Figure 4. The Agilent ICP Expert software provides a clear workflow to guide
you through method development and analysis.
Video help
Comprehensive video help is included with the ICP
Expert software (Figure 5) — so you can get assistance
on demand. The video help shows you how to change
and use sample introduction components for different
applications, through to how to complete routine
maintenance tasks.
Figure 5. On demand help videos for sample introduction system components
and routine maintenance tasks.
3
Accessory control
The Agilent SPS 4 Autosampler is fully controlled
through ICP Expert software. The user can select
the sample rack type, rinse pump control speed and
control the probe through the autosampler window or
autosampler tab in ICP Expert. Control of the IsoMist
programmable temperature controlled spray chamber is
also fully integrated into the ICP Expert Software.
Control of the Advanced Valve System (AVS) 6 or 7
port switching valves is fully integrated and controlled
through the ICP Expert software via the optional Pro
Pack software module (the 4 port version is supported
by the base software). This ensures optimal timing,
unlike third party switching valve accessories with
complicated, stand-alone control software. Parameter
settings, integrated into ICP Expert software for the AVS
6/7 are displayed in Figure 6. The software incorporates
the AVS parameter calculator to facilitate the setup and
method development of the AVS 6/7 (Figure 6).
Figure 6. The parameter calculator and setup conditions for the AVS 6/7
Switching Valve makes instrument setup and method development easy.
Intelligent Rinse
The Intelligent Rinse software function is available
in the ICP Expert Software Pro pack. It monitors the
intensities of nominated element wavelengths during
the rinse period, automatically ending the rinse when
these intensities reach a threshold. The threshold is
based on which rinse setting you select: Thorough,
Moderate, or Quick. This allows the rinse period to
vary, depending on the time taken to wash out each
individual sample. This improves sample throughput
whilst preserving the accuracy of results.
Integrated control of ESI prepFAST™
The 5100/5110 ICP-OES can be coupled with the ESI
prepFAST autodilution system. This will enable the
following labour saving functions:
• Multipoint calibration from a single stock
standard—no need to manually make up standards,
saving time and avoiding mistakes
• Automatic overange dilution—when a sample is
detected as being outside the calibration range, a
dilution will automatically be performed
IntelliQuant Calibration Kit. For full flexibility, a custom
IntelliQuant calibration can be measured and stored for
particular sample matrices, such as organic solvents.
Any updated or new IntelliQuant calibration is measured
in the standard quantitative analytical sequence,
meaning no separate analyses or additional worksheets
are required.
The IntelliQuant interface features a periodic table heat
map that shows which elements have been found in a
sample and their approximate concentration (Figure 7).

• Prescriptive dilution—setup the method to perform

a dilution as soon as it gets to the sample.

Rapid sample assessment and simplified method

development using IntelliQuant

Agilent’s IntelliQuant is a feature in the ICP Expert
software that identifies and calculates an approximate
concentration (semi-quantifies) of up to 70 elements
in a sample. This enables users to quickly check the
approximate concentration of many elements in their
sample, without any changes to the method or preparing
additional multi-element standards. IntelliQuant
measurements can be performed with every analysis.
For stored calibrations, simply activate IntelliQuant and
select the stored IQ calibration file before running an
analysis.
Figure 7. IntelliQuant generates a heat map to visually present the relative
concentrations of elements present in a sample.
A full wavelength spectrum (Figure 8) is also displayed
after the scan, providing more information about the
elements present and any potential interferences. You
can zoom in on the spectrum for further detail. Changes
can be made to the IntelliQuant setup with results
reprocessed in real-time, saving valuable time during
method development or analysis.

In addition to the standard detector read for quantifying

the selected elements in a sample, IntelliQuant takes a
full wavelength scan from 167 to 785 nm. The end result
is both the quantitation of the elements defined in the
method as well as identification and semi-quantitation
of up to 70 additional elements in as little as 15
additional seconds of measurement time per sample.

Users have the option of default IntelliQuant calibrations

(factory calibrations without the need to analyze any
additional standards) or, for more accurate results, it is
possible to update the default IntelliQuant calibrations
by using a set of multi-elemental standards in Agilent’s

4
Figure 8. The IntelliQuant interface displays the full wavelength scan of the sample (top) and a detailed spectrum view of any selected wavelength range from
the full scan, which includes labeled element peaks (bottom)

Correction options to suit any situation
The Agilent ICP Expert software provides many choices
of correction options for both background or spectral
interferences:
• Fitted background correction: utilizes a
sophisticated mathematical algorithm to model
the background signal under the analyte peak and
is applied simultaneously upon measurement of
the analyte peak, providing fast, easy and accurate
background correction. ‘Fitted’ not only provides
accurate correction of both simple and complex
background structures, it also requires no method
development.
FACT provides real-time spectral correction by
using an advanced spectral modelling technique
to mathematically deconvolute (i.e. separate) the
analyte signal from the raw spectrum. Models
are built by measuring the expected components
separately and the response for each. This typically
includes measuring a blank solution, a pure
analyte solution and pure interferent solutions.
Figure 9 displays a FACT model being applied to
Cd 214.439, with Fe interferent, with FACT being
able to mathematically separate the two peaks to
accurately measure the analyte signal.
For overlapping interferences that cannot be
resolved using Fitted background correction or
FACT, Inter-element correction (IEC) is available.

• Off-peak correction: is a traditional correction

technique that allows you to manually select
correction points —from left, right or both sides of
the analytical peak.

• FACT correction: Agilent’s proprietary Fast

Automated Curve-fitting Technique (FACT) applies
a highly sophisticated, yet easy-to-use spectral
modeling technique to accurately model complex
analytical spectra often observed when analyzing
challenging sample matrices by ICP-OES.
FACT is a powerful alternative to inter-element
correction (IEC). It also offers the capability of
accurate background correction, particularly
useful when highly-complex background structure Figure 9. Example of a FACT model being applied to Cd 214.439 nm, with
interference from Fe. A solution containing ~ 100 µg/L Cd in 10,000 mg/L Fe
is observed and more traditional background was analyzed on an Agilent 5110 ICP-OES.
correction techniques are unsuitable.
5
The background correction type for each analyte is
easily edited after analysis via the spectrum display
(Figure 10).
for that wavelength. This enables you to extend the
dynamic range of your determination from parts per
billion to percentage levels. If desired, you can overlap
the concentration ranges, giving you a simple way to
verify your sample results.

Figure 10. Background correction options are flexible and easily edited post-
analysis, recalculated in real-time.

Extended dynamic range

The MultiCal feature of the software enables the user to

setup and measure multiple calibration ranges for their
analysis, increasing the linear dynamic range.

Elements generally have more than one wavelength

at which they can be measured. These wavelengths
have different sensitivities and by using a combination Figure 11. Use MultiCal to extend dynamic range and achieve accurate
of these wavelengths, you can extend the dynamic results from a single sample measurement. In this example, Zn 213.857 nm
is measured over the range 0 to 5 ppm and Zn 472.215 nm covers higher
range of your determination. Using one calibration for concentrations up to 55 ppm.
your analysis may limit the range over which you can
accurately determine the amount of an element present Reporting versatility
in a batch of samples, covering an extensive calibration
range. Selecting the most sensitive line will give the
best detection limits and accurate determinations of
low-level analytes whereas selecting less sensitive
lines will allow the measurement of the higher analyte
concentrations, in the same determination. The MultiCal
feature requires only a few standards to define the
calibration range for each wavelength. Figure 11
displays two calibration graphs. The first calibration
displays a sensitive line with a concentration range
of 0–5 ppm. The second calibration displays a less
sensitive line with a concentration range of 0–55 ppm,
measuring Zn over a wider concentration range.

Based on the measured signal intensity, MultiCal

automatically reports the result based on the calibration Figure 12. Agilent ICP Expert contains a variety of reporting options to meet
your needs, created using the Report Designer.
6
The Agilent ICP Expert software provides you with all
the reporting versatility you need.
The Report Designer enables you to configure reports
to include only the data you require, and to customize
report layout with your company logo. Once designed,
reports can also be saved as templates, so setup need
only be done once.
Expanded QC support for USP <232>/<233> and
ICH Q3D methods
The ICP Expert software supports laboratory compliance
with the USP and ICH requirements for measuring
elemental impurities in pharmaceutical products and
their components.
Software features include:
• J-value Spike Calculator
• pass/fail acceptance criteria limits flagged for
validation tests and components
• can operate within 21 CFR 11 software module
• facilitate user setup to validate and run method in
compliance with USP<232>/<233> and ICH QRD
• supplied method templates to accelerate method
development
Data transfer and export
Results are easily transferred to a LIMS or other
application using the data exporting options offered in
the ICP Expert Software.
Data can be exported in CSV format for direct transfer to
a spreadsheet or other program for data manipulation,
trend analysis, or process monitoring. The multi-
tasking capabilities of the ICP Expert software allow
the simultaneous reviewing, processing and generation
of reports on analyses that have just been completed,
while also using the Agilent 5110 ICP-OES to collect
data for the current batch of samples.
7
Base and Pro ICP Expert Software
The ICP Expert software is available in two versions:
Base and Pro.
The Base Software pack features all the necessary
functionality for the everyday operation of a routine lab.
The Pro Software feature pack offers additional features
to enhance productivity and functionality including:
• Easy-to-use rate driven Quality Control
• Export to MS Excel
• Control of selected 3rd party autosamplers
• Trend monitoring of argon back pressure to the
nebulizer and argon emission per solution
• Oxygen addition and nitrogen purge
• Control of the Advanced Valve System switching
valve accessory (AVS 4, 6 or 7)
• Integrated Control of ESI prepFAST autodilution
system
• Intelligent Rinse
• Ability to customize replicates
ICP Expert software for 21 CFR 11 compliance
An optional 21 CFR 11 extension pack, compatible
with the Pro version of ICP Expert, is available with
Spectroscopy Configuration Manager (SCM) software
that assists with compliance to the US FDA 21 CFR
Part 11 requirements. It provides support for assigning
user access privileges, creating audit trails, electronic
records and electronic signatures.
Easy troubleshooting
As shown in Figure 13, virtual LEDs displayed on the
Instrument Status page provide an immediate overview
of the current status of the system – allowing you to
quickly check the instrument status or troubleshoot the
system in the unlikely event of an instrument problem.
The interactive diagram of the Agilent 5110 ICP-OES
system, gives details on the instrument status and
suggests the possible causes of error. For more detailed
guidance, the instrument dashboard provides live
information on various instrument parameters, making
it easy to find and correct problems. The current status
is automatically updated when the system changes
(Figure 14).
Figure 13. View the interactive Instrument Status page to check the current
status of the instrument and identify causes of problems
Figure 14. The Instrument Dashboard displays gauges to monitor instrument
parameters
Checking instrument performance
For periodic performance testing of the instrument,
a suite of tests is available to automatically verify
instrument performance. The instrument tests check
the basic functionality of the instrument, to confirm
the system is working to specification. This can be
an important troubleshooting tool. Poor instrument
setup, especially of water, gas, exhaust and sample
introduction systems is often the cause of poor
instrument performance or instrument failure. At the
completion of the test cycle, a report in PDF format can
be exported.
Figure 15. Periodically run instrument tests to confirm the instrument is
working to specification. A report can be exported to PDF format
References
Fitted Background Correction (FBC)— fast, accurate and
fully-automated background correction Technical Overview,
Publication number- 5991-4836EN
Real-time spectral correction of complex samples using
FACT spectral deconvolution software, Technical Overview,
Publication number- 5991-4837EN
Rapid sample assessment and simplified method
development with IntelliQuant Technical Overview, Agilent
Publication, 5991-6876EN
www.agilent.com/chem
Agilent shall not be liable for errors contained herein or for
incidental or consequential damages in connection with
the furnishing, performance or use of this material.
Information, descriptions, and specifications in this publication
are subject to change without notice.
© Agilent Technologies, Inc. 2017
Published October 1, 2017
Publication number: 5991-7720EN
Rapid sample screening and simplified
method development with IntelliQuant

Technical Overview

Introduction
In addition to routine quantitative elemental analyses using ICP-OES,
analytical laboratories often want to quickly identify other elements in their
samples. For example:
• Food testing labs analyzing a routine set of elements want to be aware
of the presence of additional elements that may be of concern from a
quality control perspective

• Mineral testing labs want to be sure no unexpected precious metal

group (PMG) or other valuable metals go unnoticed

• Analysts developing ICP-OES methods need a screening tool that

identifies all elements present in their sample to simplify method
development
Agilent’s IntelliQuant is a software feature that identifies and calculates
an approximate concentration (semi-quantifies) for up to 70 elements in a
sample, expanding the capabilities of a classic ICP-OES analysis.
Using IntelliQuant, analysts can quickly check the
approximate concentration of many elements in their
sample, without changing their routine methods or
preparing additional multi-element standards, which can
be technically challenging and time consuming.
IntelliQuant is available on all Agilent 5110 ICP-OES and
5100 ICP-OES systems and is fully integrated into ICP
Expert software (V7.3 and later).
How does IntelliQuant work?
Identify up to 70 additional elements in one fast full
wavelength scan
In addition to the standard detector read for quantifying
selected elements in a sample, IntelliQuant can take a
full wavelength scan from 167 to 785 nm. The end result
is both the quantitation of the elements defined in the
method as well as identification and semi-quantitation
of up to 70 additional elements—all with just two
readings of Agilent’s VistaChip II CCD detector and as
little as 15 additional seconds of measurement time per
sample.
Default or custom calibration
IntelliQuant includes default calibrations, available for
axial, radial and Synchronous Vertical Dual View modes.
These factory calibrations allow the estimation of up to
70 elements in a sample without the need to analyze
any additional standards.
If you want more accurate results without additional
sample preparation, it’s possible to update the default
IntelliQuant calibrations by using a set of multi-element
standards in Agilent’s IntelliQuant Calibration Kit.
This kit includes multi-element standards which are
measured to update the default IntelliQuant calibration
for all 70 elements, which is ideal for labs requiring
more accurate semi-quantitative analysis without time
consuming standard preparation.
For full flexibility a custom IntelliQuant calibration can
be measured and stored. This calibration can be fully
customized to contain any set of elements over the
desired calibration range.
2
This feature allows you to create a calibration from
standards that are matrix-matched to your samples,
giving better accuracy.
Any updated or new IntelliQuant calibration is measured
in the standard quantitative analytical sequence,
meaning no separate analyses or additional worksheets
are required.
What does IntelliQuant allow you to do?
Perform rapid screening
IntelliQuant is an ideal rapid sample screening tool.
It identifies and semi-quantifies up to 70 unknown
elements and takes as little as 15 additional seconds
per sample.
The IntelliQuant interface features a periodic table
heat map that shows which elements have been
found in a sample (Figure 1). Elements present at
low concentrations are coloured yellow, medium
concentration are orange and high concentration are
red.
Figure 1. IntelliQuant generates a heatmap to visually present the relative
concentrations of elements present in a sample
The heat map is easily customizable. For example, a
food testing laboratory may set the high concentration
limit as the maximum regulated concentration for a
specific element, so non-compliant samples will be
displayed with red on the periodic table.
A full wavelength spectrum (Figure 2) is also displayed
after the scan, providing more information about the
elements present and any potential intereferences. You
can zoom in on the spectrum for further detail.
Figure 2. The IntelliQuant interface displays the full wavelength scan of the sample (top) and a detailed spectrum view of any selected wavelength range from
the full scan, which includes labeled element peaks (bottom).

Simplify method development Address unexpected results

When developing an ICP-OES method it’s useful to
know if any unknown elements are present, as they
may cause spectral interferences with the elements
of interest. The full scan obtained by IntelliQuant
can guide wavelength selection, avoiding such
interferences, thus simplifying the method development
process.
IntelliQuant is also useful when the elements in a
sample are completely unknown. The full wavelength
scan generated can be used to determine which
elements are present, allowing the analyst to then
decide which should be quantitatively analyzed in a full
ICP-OES analysis.
As the instrument reads all wavelengths for all elements
during an IntelliQuant analysis, any wavelength used
for semi-quantitative analysis can be changed after
the analytical run is complete. This allows you to
retrospectively analyze data at any time after the initial
analysis.
For example, if a spectral interference is observed in an
IntelliQuant spectrum an alternative wavelength can be
selected and the concentration of the element is quickly
and automatically reprocessed.

IntelliQuant data can also be used to identify

appropriate calibration ranges for each element.

3
Example analysis Table 1. IntelliQuant semi-quantitative recoveries for elements in two food
CRMs.

Five pre-digested Certified Reference Materials (CRMs), Orchard Leaves Powdered Milk Solution
representing a range of sample types were analyzed (CRM-OL) (CRM-MP)

using IntelliQuant on an Agilent 5110 SVDV ICP-OES. Element & CRM IntelliQuant CRM IntelliQuant
wavelength Value Value Value Value
Results were obtained using an updated IntelliQuant (nm) (µg/mL) (µg/mL) (µg/mL) (µg/mL)
calibration, with the IntelliQuant Calibration Kit multi- Aluminum
element standards being measured on the instrument 237.312 3 3 0.02 –
during a standard analytical run. All measurements were Arsenic
188.98 0.1 0.07 0.002 –
taken in the Synchronous Vertical Dual View (SVDV) Barium
mode of the instrument. 614.171 0.5 0.5 –   –
Cadmium
214.439 0.001 – –   –
Semi-quantitative recoveries for all elements in the food
Calcium
CRMs are shown in Table 1. Results for environmental 318.127 200 204 130 136
CRMS are shown in Table 2. IntelliQuant’s retrospective Chromium
wavelength selection capabilities were utilized to avoid 205.56 0.03 0.03 0.0003 –
spectral interferences and achieve optimum semi- Cobalt
230.786 0.002 – 0.0004  –
quantitative accuracy. Copper
213.598 0.1 0.09 0.007 – 
By changing the wavelengths used to estimate Iron
the concentrate of those elements that were being 273.358 3 3 0.02  –
Lead
impacted by interference, it was possible to achieve 217.000 0.5 0.5 0.002 – 
recoveries for almost all measured elements in the five Magnesium
CRMs within ± 20% of the certified value. The accuracy 277.983 60 57 12 12
of these results demonstrate IntelliQuant’s potential as Manganese
293.931 1 1 0.003  –
an extremely useful rapid screening tool. Nickel
231.604 0.01 –  –  –
References Phosphorus
177.434 20 20 100 99
Potassium
CCD and CID solid-state detectors, Agilent publication 766.491 150 140 170 153
5991-4854EN, (2014). Selenium
196.026 0.0008 – 0.001  –
Silicon
243.515 5 7  –  ––
Sodium
589.592 1 1 50 44
Vanadium
311.837 0.005 –  –  –
Zinc
334.502 25 30 0.5 0.6

4
Table 2. IntelliQuant semi-quantitative recoveries for elements in three environmental CRMs.

Estuarian Sediment Solution Soil Solution B Soil Solution A

(CRM-ES) (CRM-SOIL-B) (CRM-SOIL-A)
Element & CRM Value IntelliQuant Value CRM Value IntelliQuant Value CRM Value IntelliQuant Value
wavelength (µg/mL) (µg/mL) (µg/mL) (µg/mL) (µg/mL) (µg/mL)
(nm)
Aluminum 237.312 700 705 700 720 500 534
Antimony 206.834 0.004 –  0.4 0.3 0.03  –
Arsenic 188.98 0.1  – 6 5 0.2 0.1
Barium 614.171  – –  7 6.48 5 5
Beryllium 313.107 0.02 0.04 – –  –  –
Cadmium 214.439 0.0004  – 0.2 0.2 0.003 – 
Calcium 318.127 80 75 125 118 350 331
Chromium 205.56 0.8 0.8 0.4 0.4  –  –
Cobalt 230.786 0.1 0.1 0.1 0.1  –  –
Copper 213.598 0.2 0.2 3 3 0.3 0.3
Iron 273.358 350 288 350 309 200 189
Lead 217.000 0.3  – 60 58 0.4 0.3
Magnesium 277.983 100 89 80 74 70 62
Manganese 293.931 4 4 100 89 0.1 0.2
Nickel 231.604 0.3 0.3 0.2 0.2 0.3 0.3
Phosphorus 177.434 5 5 10 10 10 9
Potassium 766.491 150 150 210 189 200 192
Selenium 196.026 0.05  – – – 0.01 – 
Silicon 243.515 3000 2430 3000 2460 3000 2390
Sodium 589.592 200 146 100 86 70 65
Thorium 283.73 0.1  – 0.1 – 0.1  –
Uranium 385.957  –  – 0.25 – 0.01 – 
Vanadium 311.837 1 0.9 0.8 0.7 0.1 0.09
Zinc 334.502 1.5 1.6 70 74 1 1

5
 www.agilent.com/chem
Agilent shall not be liable for errors contained herein or for
incidental or consequential damages in connection with
the furnishing, performance or use of this material.

Information, descriptions, and specifications in this publication

are subject to change without notice.

© Agilent Technologies, Inc. 2016

Published May 1, 2016
Publication number: 5991-6876EN

IntelliQuant generates a heatmap to visually represent
the relative concentrations of elements present in
a sample.
The entire spectrum from 167 to 785 nm can be quickly
viewed with IntelliQuant, allowing likely analytes and
interferents for a specific sample to be determined.
* Available on Agilent 5100 and 5110 ICP-OES instruments
with ICP Expert software version 7.3 or later
This information is subject to change without notice.
© Agilent Technologies, Inc. 2017
Published in the USA, October 1, 2017
5991-8455EN
IntelliQuant for ICP-OES
Automatic full spectrum scan and simplified
method development
Get a quick approximation of the concentration of up to 70
elements per sample
Agilent’s IntelliQuant* is a software feature that calculates an approximate
concentration (semi-quantifies) of up to 70 elements in a sample. It adds only
seconds to a sample measurement and requires no calibration. IntelliQuant:
–– Collects the entire wavelength range from 167 to 785 nm in seconds,
discovering every element in your sample
–– Has inbuilt calibrations for the three viewing modes of the 5100/5110
ICP-OES: axial, radial and synchronous vertical dual view
–– Has fast data visulization with results displayed in a color-coded periodic
table heat map
–– Simplifies method development and quickly identifies potential
interferences in your sample, making wavelength selection much easier
–– Can also be used to identify appropriate calibration ranges for each
element.
Accuracy can be increased for complex matrices by creating matrix matched
calibrations, using Agilent's multi-element calibration solutions.
For more information visit:
www.agilent.com/chem/5110icpoes

Benefits of Agilent's Intelligent Rinse
–– Shorter rinse durations between
samples
–– Increased sample throughput
–– Simplified method setup–no need
to accurately predict required rinse
durations
Agilent's Intelligent Rinse function adjusts rinse
time, based on the time taken to wash out each
element in your sample. In this example, Intelligent
Rinse saved over two minutes of unnecessary
rinse time when measuring samples at the low end
of the calibration compared with those at higher
concentrations.
* Available on Agilent 5100 and 5110 ICP-OES instruments
with ICP Expert software version 7.4 or later
This information is subject to change without notice.
© Agilent Technologies, Inc. 2017
Published in the USA, October 1, 2017
5991-8456EN
Intelligent Rinse for ICP-OES
Increase productivity and reduce errors
Intelligent Rinse* maximises throughput by optimising the rinse
time between samples
When setting up an ICP-OES method, you are normally forced to specify a
rinse time that will suit your highest concentration samples. This rinse time is
then used for all samples, even those with much lower concentrations of the
analytes.
Rinse times depend on several factors, such as the type and length of the
sample introduction tubing, the specific sample introduction components
used and the nature of the samples and elements being analysed. As
shown in the figure, samples with low concentrations of an element take a
shorter time to wash out than higher concentrations—with over two minutes
difference between the highest and lowest concentrations shown. If your
samples have a large variation in concentrations, using the same rinse time
for each can add considerable time to the analytical run.
Agilent's Intelligent Rinse software function monitors the intensities of
nominated element wavelengths during the rinse period, automatically
ending the rinse when these intensities reach a user-specified threshold. This
allows the rinse period to vary, depending on the time taken to wash out each
individual sample. This increases sample throughput whilst preserving the
accuracy of results.
For more information, visit:
www.agilent.com/chem/5110icpoes

With automatic dilution in place, the internal
standard (Y) and Na and Ca recoveries were
dramatically improved.
Automate the preparation of calibration standards
to create calibrations like this - with a correlation
coefficent of 1.00.
* Available on Agilent 5100 and 5110 ICP-OES instruments with
ICP Expert software version 7.4 or later
This information is subject to change without notice.
© Agilent Technologies, Inc. 2017
Published in the USA, November 1, 2017
5991-8481EN
Auto-dilution for ICP-OES
Boost productivity and eliminate tedious
sample preparation
No more standards and sample preparation
Agilent’s ICP-OES instruments*, in combination with the ESI prepFAST™
autodilution system, can automate a number of tedious sample preparation
processes. Preparing calibration curves, diluting samples and compensating
for internal standard suppression can all be automated.
Overrange dilution
The ICP-OES/prepFAST autodilution system provides a range of functions
to reduce the manual effort involved in analysing samples which are
unexpectedly over the calibrated range, including a Reactive Dilution feature
that performs automatic dilutions on overrange samples, using a dilution
factor of up to 400x.
Reduce sample preparation effort
As well as diluting overrange samples, the ICP-OES/prepFAST autodilution
system can automatically dilute samples prior to analysis. This saves
considerable analyst time, allowing them to focus on other tasks.
Automatic calibration preparation
Multi-point, multielement calibration curves can be automatically created from
a single stock solution as part of an analytical run. This removes the need for
complicated, error-prone manual standard preparation.
Compensate for Internal Standard suppression
If internal standard measurements fall outside specifed recovery thresholds
samples can be automatically diluted. This increases measurement accuracy
without sacrificing productivity. The figure (top left) shows the suppression of
internal standard (Y) recovery to 82% in the undiluted sample. The dependent
analytes (Na, Ca) gave correspondingly poor recoveries. Dilution improved the
internal standard recovery to 97% and the dependent analytes were measured
at <5% variance from the expected values.
For more information, visit:
www.agilent.com/chem/5110icpoes

Pass/Fail results are visually displayed for each
element, allowing you to quickly see which samples are
within the impurity limits.
The Spike Calculator feature in the ICP Expert
software.
Simplified QC for
Pharmaceutical Elemental
Impurity Analysis
For ICP-OES
Expanded support for USP <232>/<233> and ICH Q3D methods
supports compliance with elemental impurity analysis in
pharmaceutical materials
The US Pharmacopeia (USP) and the International Council for Harmonization
of Technical Requirements for Pharmaceuticals for Human Use (ICH) have
released procedures that provide specific, quantitative determination of
individual elemental impurities in drug products and ingredients. These
procedures reference ICP-MS and ICP-OES as the suggested analytical
techniques.
Agilent’s ICP Expert software* for the 5100 and 5110 ICP-OES instruments
offers the following features that support compliance with the USP and ICH
procedures.
Method validation tools
Pass/fail limits for acceptance criteria are flagged in the ICP Expert software.
This includes the following validation tests for drug products:
• Accuracy
• Repeatability
• Detectability
• Ruggedness
The validation tests are easy-to-use and setup, with a pass or fail given for
every element and wavelength, no calculations are required.
Spike calculator
• Facilitates setup and method development: helps to define calibration
concentration levels and QC spike concentrations, based on “J-value”—
the maximum permitted concentration limit for the analyte in a sample,
corrected for sample preparation dilution.
• No calculations required: the maximum permitted concentration limits for
analytes in a given sample are automatically applied
PDE Limits
Upon activation of USP/ICH specific support, permitted daily exposure levels
(PDEs) are pre-populated for all the target analytes covered in USP <232>
and ICH Q3D. This prevents the possible transcription errors associated with
entering/transferring the values into the software.
Supercharge your elemental impurity analysis
Move from installation to productive analysis quicker for your USP <232>, USP
<233> and ICH Q3D analysis by starting with the purpose-designed template
supplied with the ICP Expert software. This template has all the target
analytes and spike solutions prepopulated to save method development time. Pre-populated PDE values are visible from the QC page.

21 CFR part 11 compliant

The ICP Expert expanded QC functionality is 21 CFR part 11 compliant via the
optional 21 CFR 11 extension pack. This compatible with the Pro version of
ICP Expert that includes; the Agilent Spectroscopy Database Administrator
(SDA); and Agilent Spectroscopy Configuration Manager (SCM) software. The
pack is qualified by Agilent as complying with the requirements of:
• 21 CFR 58 (Good Laboratory Practice)
• 21 CFR 210 (Good Manufacturing Practice for Drugs),
• or 21 CFR 211 (current Good Manufacturing Practice for finished
pharmaceuticals)

Purpose-designed template for the USP/ICH Q3D

quantitative procedure for elemental impurities
analysis with detectability test selected.

For more information visit:

www.agilent.com/chem/5110icpoes
* Available on Agilent 5100 and 5110 ICP-OES
instruments with ICP Expert software version 7.4
or later

This information is subject to change without notice.

© Agilent Technologies, Inc. 2017

Published in the USA, October 1, 2017
5991-8567EN
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This information is subject to change without notice.

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