Name: Kim Suhyr Bagis Batausa

Yr&sec: BSN II-F

ACTIVITY NO: 10

Objective: to be able to obtain blood sample through venipunture for blood chemistry
analysis and to know the different kinds of collecting tubes

Material:
syringe
Sterile cotton balls
Tourniquet
Blood collecting tubes
Isopropyl alcohol

Procedure:
Step by step instructions to obtain blood from a vein.
1. Prepare needle and syringe apply tourniquet to upper arm.
2. Immediately ask the patients to open and close his first several times simultaneously.
3. Cleanse skin of selected puncture site with alcohol and let dry.
4. “Fix” the vein by grasping the patients arm below the level of the elbow and pulling
the skin test then insert needle vein at approximately 25®angle.
5. Now ask the patient to relax his arm completely and to keep his hand open. Release
tourniquet presses an alcohol sponge over wound and simultaneously remove the needle
elevate the patients arm.
6. Detach needle from step of syringe and carefully transfer blood to collection tube. If an
anticoagulant is required add this to the blood immediately and mix gently but
thoroughly.
7. Label specimen with care includes patient’s full name, date, and any other info.
Customarily required by the laboratory.
8. Unless disposable equipment is used rinse both needle and syringe with tap water
(alcohol must never be used for his purpose) before the blood has chance to harden.

Observations:
1. What are the common methods used in collecting a blood sample and give the 2
common sites used?
♦ Venipunture
♦ Capillary

2. Enumerate at least 5 tests that can be performed using each methods of collection?
 Blood culture
 Blood bag system
 Coagulant blood test
 Anticoagulant blood test
 Blood type exam
3. Enumerate at least 6 different kinds of collecting tubes and its usage with the color
code and may show in diagram?

Red Top

ADDITIVE None
MODE OF Blood clots, and the serum is separated by
ACTION centrifugation
Chemistries, Immunology and Serology, Blood Bank
USES
(Crossmatch)

Gold Top

ADDITIVE None
Serum separator tube (SST) contains a gel at the
MODE OF
bottom to separate blood from serum on
ACTION
centrifugation
USES Chemistries, Immunology and Serology

Light Green
Top

ADDITIVE Plasma Separating Tube (PST) with Lithium heparin

MODE OF Anticoagulates with lithium heparin; Plasma is
ACTION separated with PST gel at the bottom of the tube
USES Chemistries

Purple Top
ADDITIVE EDTA

MODE OF
Forms calcium salts to remove calcium
ACTION

Hematology (CBC) and Blood Bank (Crossmatch);

USES requires full draw - invert 8 times to prevent clotting
and platelet clumping

Light Brown
Top

ADDITIVE Sodium heparin

MODE OF Inactivates thrombin and thromboplastin; contains

ACTION virtually no lead

USES Serum lead determination

White Top

ADDITIVE Potassium EDTA

MODE OF
Forms calcium salts
ACTION

USES Molecular/PCR and bDNA testing

Conclusion: