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Bioscience Discovery, 9(3):337-339, July - 2018

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ISSN: 2229-3469 (Print); ISSN: 2231-024X (Online)
Research Article
Eco-friendly management of root wilt of chickpea and pigeon pea by
using plant leaf extract
Ingale S.T.* and S.S. Patale

Smt. S. K. Gandhi Arts, Amolak Science and P.H Gandhi Commerce College, Kada,Tq. Ashti, Dist. Beed.
414202 (M.S.) India Ph. 9823937501

Article Info Abstract

Received: 18-04-2018,
Antifungal activity of Azardirachta indica L., Jatropha curcus L., Datura
Revised: 01-06-2018, stramonium L. and Annona squamosa L. were assessed against Fusarium wilt of
Accepted: 22-06-2018 chickpea and pigeon pea (Fusarium oxysporum f. sp. ciceri and F. oxysporum f.
sp. udum) Invitro. Leaf extract of selected medicinal plant preparing various
concentrations viz. 5, 10, 15 and 20 percent were tested against pathogenic fungi
Antifungal activity, disease of selected pulses by food poisoning method. As concentration increases
management, food inhibitory activity also increases. Aqueous leaf extract of J. curcus shows
poisoning method, leaf maximum inhibitory effect on tested pathogens followed by A. indica and A.
extract, Pathogenic fungi. squamosa.

INTRODUCTION Jatropha curcus leaves extracts which are able to

Fusarium sp. is the most important and severe plant control the anthracnose disease in three banana
pathogenic fungi. It is infect any stage of growth varieties: ‘Robusta’, ‘Rasthali’ and ‘Ney Poovan’.
and reduce total production of respective plant. The use of medicinal plant materials for the
After harvesting the plant it is survive on the plant inhibition of fungal diseases is an old practice in
trashes and soil. It infect also in post harvest stage. many countries and is still offers an enormous
Medicinal plants represent a rich source of potential source of antifungal agent (Usharani and
antimicrobial agents (Mahesh and Satish, 2008). Chitra, 2014). Many phyto-chemical pesticides
Many of the plant materials used in traditional exhibiting broad spectrum of activity against pest
medicine which are readily available in rural areas and diseases have long been considered as attractive
at relatively cheaper than modern medicine (Mann alternative to synthetic chemical pesticides as they
et al., 2008). The use of biological compounds are biodegradable, target specific and pose no or
extracted from plants may be an alternative to less hazard to the environment or to human health.
conventionally used fungicides to control (Walia et al., 2014).
phytopathogenic fungi, due to their being bioactive Plants have been a rich source of medicines
chemicals such as flavonoids, phenols, tannins, because the produce a bioactive molecules, most of
alkaloids, quinones, saponins and sterols (Burt, which probably evolved as chemical defenses
2004). against predation or infection (Anitha et al., 2016).
In recent year a number of plant extract The testing of the efficacy of such potential plant
their essential oils and their volatile components based sources for antifungal activity could an
have been reported to have strong antifungal important step towards the assessment of the degree
activity (Siripornvisal, 2009). According to of variability among the diverse natural flora
(Thangavelu et al., 2004), the mycelial growth of (Manoorkar and Gachande, 2014).
Colletotrichum musae was inhibited by the 337 ISSN: 2231-024X (Online)
Ingale and Patale
MATERIALS AND METHODS: 100ml, 10gm in 100ml, 15gm in 100 ml and
Isolation of pathogenic fungi: autoclave it for 40 min at 1200c.then extract was
Infected plant of chickpea and pigeon pea were filtered through double layered muslin cloth and
brought to laboratory and infected part cut with help finally through Whatman filter paper no. 1 and
of sterilized blade and washed with water then filtrate was used for further investigation and stored
surface sterilization placed in 2 min in HgCl2 and in pre-sterilized flask.
washed it 2-3 times with distilled water infected Bioassay of pathogenic fungi:
pieces was placed of PDA plate and incubate at Bioassay was carried out in Czapek Dox broth
room temperature. Each fungal colony separated at medium. 10ml of leaf extract mixed with 10ml
fifth day and making pure culture and observed medium in 100ml sterilized conical flask and same
under microscope, on the basis of their colony quantity of water received for control. Seven days
color, shape, size, conidia and mycelium and fresh culture inoculums disk of fungal pathogen
identified by using manual of The Illustration of transferred in flask containing medium with and
Fungi (Mukadam D.S. et al., 2006) without extract. The flask were incubated at room
temperature for 10-12 days on incubation the
Collection of plant extracts: mycelial biomass in each treatment was collected
Fresh healthy leaves of D. stramonium and J. on pre-weighted filter paper and dry weight was
curcus L. were collected from different location of determined after 24 hrs oven drying at 600 c. The
Beed and Ahemdnagar district and washed with dry weight of the mycelia was determined by
HgCl2 for 2 min after that washed thoroughly 2-3 subtracting the weight of the filter paper from the
times with distilled water then leaves were grinded total weight of the filter paper with mycelia. Three
in mixture and obtained fine soft powder. replicates were maintained for each treatment.
Preparation of plant extract: Percentage of inhibition was calculated by the
Obtained powder of leaves is weighted and mixed formula, (Edington et al., 1971, Vincent, 1947)
with different level of concentration i.e. 5gm in

RESULT AND DISCUSSION: lunata, Mucor sp., Cladosporium, Alternaria

Antifungal activity of four medicinal plant leaf helianthi and Rhizopus nigricans. (Jalander and
extract tested exhibited different degrees of Gachande, 2011) with plant leaf extract from
antifungal activity shown by J. curcus on F. Tinospora cordifolia against Fusarium oxysporum
oxysporum f. sp. udum (84.00) followed by A. and Alternaria solani. (Jalander and Gachande,
indica (78.12) and in the case of F. oxysporum f sp. 2012) four sp. of Datura stramonium, D. metal, D.
ciceri also J. curcus shown (77.77) followed by A. ferox, D. innoxia against Fusarium oxysporum (wilt
squamosa (77.22). The present study has shown that of pigeon pea), Alternaria solani early blight of
the extract of J. curcus and A. indica posses tomato.
remarkable antifungal activity against many
pathogenic fungi. Similar studies have been carried ACKNOWLEDGEMENT:
out by different researchers on antifungal activity of The first author thanks to the Director of Dr.
respective plant extract. The correlated study done Babasaheb Ambedkar Research and Training
by (Hussain et al., 1992) reported the leaf extract Institute Pune for the award of Savitribai Phule
of D. stramonium reduced the development of rust National Research Fellowship and the support of
pustules on the leaves of wheat. (Mumtaz and financial assistance.
Sumia 2017) Antifungal activity Withania
somnifera, Lantana camera, Aloe vera, Mentha REFERENCES:
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Bioscience Discovery, 9(3):337-339, July - 2018

Table no. 1. Effect of aqueous plant leaf extract on pathogenic fungi of chickpea and pigeon pea.

Plant species Pathogen Mycelial wt. of pathogen (mg) and % of inhibition

Control 5% 10% 15% 20%
A. Indica L. F. oxysporum f. 54 32 28 23 17
sp. ciceri (0.00) (40.74) (48.14) (57.40) (68.51)
F. oxysporum f. 64 29 23 18 14
sp. udum (0.00) (54.68) (64.04) (71.87) (78.12)
J. curcus L. F. oxysporum f. 27 10 9 8 6
sp. ciceri (0.00) (62.96) (66.66) (70.37) (77.77)*
F. oxysporum f. 25 13 7 6 4
sp. udum (0.00) (48.00) (72.00) (76.00) (84.00)*
D. F. oxysporum f. 70 59 53 50 42
stramonium sp. ciceri (0.00) (15.71) (24.28) (28.57) (40.00)
F. oxysporum f. 61 55 51 48 43
sp. udum (0.00) (9.83) (16.39) (21.31) (29.50)
A. squamosa F. oxysporum f. 101 28 26 25 23
L. sp. ciceri (00.00) (72.27) (74.25) (75.24) (77.22)
F. oxysporum f. 39 22 15 13 10
sp. udum (00.00) (43.58) (61.53) (66.66) (74.35)
*Maximum inhibition of pathogens.

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How to cite this article

Ingale S.T. and S.S. Patale, 2018. Eco-friendly management of root wilt of chickpea and pigeon pea by
using plant leaf extract. Bioscience Discovery, 9(3):337-339. 339 ISSN: 2231-024X (Online)