1. Choose the incorrect statement for second DNA strand synthesis.

a) The RNA: DNA complex is treated with the enzyme RNaseH and DNA pol
b) The enzyme RNaseH is responsible for nicking the RNA strand and leaving free 3’ hydroxyl
ends
c) These RNA fragments can be used as primers
d) There is no portion of RNA left attached to the DNA strand

Answer: d

2. What is the final product of the RNaseH method?

a) blunt ended dsDNA
b) staggered dsDNA at both ends
c) staggered dsDNA at 3’ end
d) staggered dsDNA at 5’ end

Answer: a

3. What would not happen if the RNA strand is completely removed from RNA: DNA hybrid?
a) There are no chances of the synthesis of second DNA strand
b) Chance complementarity would take place
c) Hairpin structure would be formed
d) Hairpin structure if formed is not the final structure

Answer: a

4. The loop region is single stranded. It can be cleaved by using which enzyme?
a) Exonuclease
b) S1 nuclease
c) RNaseH
d) DNase

Answer: b

5. Choose the correct statement in respect to the self priming method of cDNA synthesis.
a) It is less preferred than RNaseH method
b) A hairpin structure is formed with guarantee
c) The sequence corresponding to the 5’ end is lost
d) Reverse transciptase is not used
Answer: c

6. Choose the incorrect statement for the method homopolymer tailing.

a) The first step is the RNA: DNA hybrid synthesis
b) Terminal transferase is used for addition of nucleotides on 3’ end
c) Terminal transferase adds only at DNA strands
d) The DNA strand is now having known sequence at 3’ end

Answer: c

7. Choose the correct statement for RACE.

a) It stands for Random Amplification of cDNA ends
b) It is for cloning particular cDNA ends
c) It is only of one type, which is 5’ RACE
d) Sequence data is not available in any case

Answer: b

8. The first primer in the case of 3’ RACE is:

a) internal sequence
b) oligo-dT adaptor molecule
c) oligo-dA adaptor molecule
d) adaptor oligo-dT primer

Answer: b

9. The first cDNA strand in 5’ RACE is tailed with oligo-dA tail. Is the given statement true or
false?
a) True
b) False

Answer: a

10. What is the second primer in the case of 5’ RACE?

a) Internal primer
b) Oligo-dA sequence
c) Adaptor-oligo-dT prime
d) Oligo-dT adaptor molecule

Answer: c