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Bakteri pembusuk pada keju dihambat oleh efek asam dan garam, rendahnya kelembapan dari
keju. Spores of clostridium tyrobutyricum in the milk used for the manufacture of Emmentaler,
Edam, and Gouda can survive the heat treatment used for cheese milk and cause late gas formation
(blowing defect) and related of flavors during ripening. Thermoduric streptococcus thermophilus
can use flavor defect in Gouda cheese (45). The presence of heat stable enzymes (from
psychrotrophic bacteria) can be detrimental to the quality of both fresh and ripened cheese
(11,12,45) by causing bitter or rancid flavors and by impairing the coagulation properties of the
milk. Fresh cheeses, such as cottage cheese and other high mousture cheeses, may be subject to
spoilage by gram negative psychrotrophic bacteria (Pseudomonas, Falvobacterium, or
Alcaligenes), coliforms, and yeast and molds that eneter as post pasteurization contaminants (10).
Most hard ripened cheese are not subject to gram negative spoilage though coliform contamination
has been associated with the gassy defect in cheese making (for example , cheddar) (41). Ripeden
cheese is prone to surface growth of yeast and molds, particularly if exposed to atmospheric to the
starter cultures, culture failure, and undesirable secondary flora (45).
Cheeese Secondary Flora
Soft (surface ripened)
Camembert Yest
Brie Penicillium caselolum
Semisoft
Caephilly Lactobacilli
Limburger (surface ripened) Yeast, Brevibacterium linens
Blue vein
Roquefort Penicillium roqueforrrti, Yeast, Micrococci
Gorgonzola
Stiltor
Hard
Cheddar Lactobacilli, pediococci
Emmantaler Propionibacterium shermanii, Group D
streptococci
Gruyere Proprionibacterium shermanii, Group D
streptocci, Yeast, Coryneforms,B.linens
Microbial competititon, reduced water activity, arganic acids, and a low pH generally limit
the growth of pathogens in cheese. A slow starter culture (due to bacteriophage, antibiotics, etc).
Can allow growth of bacteria related to foodborne illnesses such as staphylococcus, Salmonella,
Listeriaa, and enteropathogenic E.coli (6, 37, 52). Which enter with raw milk or as post
pasteurization contaminants. Number of S.aureus will normally decline during the ripening stage,
but if sufficient numbers (>107 per mL) are reached during cheese making, enterotoxin may persist
in the cheese. Salmonella spp, survive beyond the ripening period (6) with the potential to cause
infection at relatively low doses (13). Most but not all, enteropathogenic strains of E.coli are
inactivated ap pH <5,0 (32) although in low acid, semisoft, surface ripened cheese, fecal coliforms
are commonly found (6). Post heating contamination or the use of contaminated raw milk can be
a source of L.monocytogenes, as was implicated in an outbreak involving a low acid Mexican style
cheese (16). Listeria is capable of surviving in cheddar, camembert and cottage cheese (69)
although growth is limited because of the low pH of most cheeses. Biogenic amine formation in
fermented dairy products has been reported by many workers. Edward and Sandine (19), reviewed
the occurrence of biogenic amines in cheeses and discussed the public health significance of the
amines. Histamines produced by the decarboxylation of histidine, and other biogenic amines such
as tyramine and tryptamine cause abdominal cramps, diarrhea, nausea, headache, palpation,
tingling, and flushing, etc. medical intervention is unnecessary, with most symptoms disappearing
a few hours after onset. Biogenic amines also affect some antidepressant drugs by counteracting
monoamine oxidase inhibitors. Enterocci and certain other lactic acid bacteria were implicated in
the production of biogenic amines in fermented dairy products.
Recommended methods
1. Yeast and Molds Counts
2. Coliforms Counts
3. Psychrotrophic count
4. (Cottage cheese)
Other methode for specific determinations
1. Staphylococcus Enterotoxin/ Thermonuclease
2. For individual pathogens such as salmonella, campylobacter, Listeria, and yersinia consult
appropriate chapters of this book.
plates can be placed in plastic bags, flushed 1 min with CO2 , sealed and incubated
at the desired temperature.
4. M16 Agar
This medium was developed to support growth of lactococci (lactic
streptococci) used in cheddar cheese manufacturing in New Zealand.
5. M17 Agar
This medium was developed by terzaghi and Sandine to support the growth of
lactococci (lactic streptococci). It is buffered with β-disodium
glycerophosphate and also is usefu for plaque assay of lactic bacteriophages. P
6. Meugon Agar
7. APT Agar
8. LBS Oxgall Agar