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1.0 OVERVIEW
Natural rubber is well known to produce various types of product. One of the
platforms which latex product most frequently used is in medical site, which is called
medical examination gloves. We used latex as raw material because of its astounding
properties, which acts as a barrier of protection both for the health care worker and the
patient to guard against contact with blood, other body fluids, and microorganisms.
Despite of the protection offered by the latex gloves, there is one little thing,
which always ignored by the users. Latex product also comes with the side effect
which is famously known as Allergic Reaction which the main priority of this project.
The project is all about to identify the chemicals, which has been the cause of the
allergic reaction to occur. After that, we are going to formulate another formulation
with the chemicals which contains the cause of the allergic reaction been minimized
with the mechanical properties of the product maintained.
With the new formulation, we can reduce the amount of chemicals, which has
been the caused of such occurrences and the new formulation did not affect the
quality of our product. This project is worth doing because if we can reduced the
effect of using latex product, the quality of health can be more secured because this is
a serious matter which is still cannot be solve until now.
Over the past few years, there has been an increasing incidence of allergic
reaction among health care workers to latex medical gloves. Current estimates on the
prevalence of latex allergy among health care workers range as high as 17%. This is
thought to be largely due to the institution of universal precautions in response to the
AIDS epidemic, and the resultant dramatically increases in glove usage.
In the process of producing latex gloves, there are various types of chemicals
which need to be include to produce a perfect gloves which can offers great barrier
properties in order to protect our skin from infection by blood contact which for the
safety both of the patient and also the health care worker (for medical platform).
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But despite of the awareness of the infection from outer source, most of the
users unaware of the chemicals that lies in the glove itself which also a serious threat
that can cause severe impact to the safety of the wearer.
1.2 OBJECTIVES
1) To perform a series of study about latex allergic reaction and the cause of such
reaction to occurs.
2) To perform studies on the chemicals used in latex glove production with the
guidelines from the supervisor.
3) To find a way to reduce the effect of allergic reaction which presents because
of the reaction between the accelerators resulting the production of latex
protein, which is the main focus in this project.
4) To reduce the allergic reaction by minimizing the amount of accelerators
usage.
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CHAPTER 2
LITERATURE REVIEW
2.0 LATEX
Latex is the common name for the fluid produced by many plants but it is the
latex that comes from the rubber tree Hevea Braziliensis that is potentially dangerous
to people who are allergic to it.
Both children and adults are affected, and children who have had repeated
surgical operations are particularly at risk. It has been suggested that some babies may
become sensitised when they come into contact with latex gloves at birth. Infants also
come across many articles of clothing, toys and other everyday items that contain
rubber, such as dummies. Any of these could also be sensitising them to latex.
Groups who are particularly at risk include health care workers and people who have
repeated surgery, because of their increased exposure to latex. The condition is most
common in atopic people (people with a tendency to develop allergies). It must be
remembered that people use latex gloves at home to do the washing up.
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2.2 TYPES OF ALLERGIC REACTION
There are two types of allergic reaction to latex. These are known as type-1 and type-
4 reactions
Type-1 allergy is potentially life threatening. Those affected are sensitive to the
natural proteins in latex. These people may suffer from nasal irritations, urticaria
(hives), asthma and anaphylaxis.
Latex allergy is unusual in that there are more than 12 allergens present in
latex. Allergy commonly involves sensitisation to different latex proteins and patients
are usually sensitive to more than one. Latex allergy is potentially a serious problem
but it can be managed and controlled. If you are sensitive to latex, it is possible you
will react to any latex product and you should try to avoid any articles made from
rubber.
Some people with relatively mild latex allergy may be able to use some latex
products. They may also be able to use some brands and not others as different
companies will use varying manufacturing processes that alter the allergenicity of the
latex protein. But even if you are slightly sensitive, it is sensible to avoid contact as
much as possible as with each contact the degree of your allergy and the reaction can
increase. People with severe allergy should not use any latex products.
4
The latex-fruit syndrome associated with latex allergy is an important issue.
Some of the proteins present in latex also exist in some fruits and some plants. If a
person is allergic to the protein in latex that is also present in bananas, for example,
they may have a cross-reaction if they eat a banana.
In some cases this works the other way around – if you are allergic to bananas,
you may be allergic to the same proteins in latex. The fresh fruits that commonly
cause problems are banana, avocado, kiwi and chestnut and occasionally other foods
including walnut. These three can cause anaphylaxis in a latex allergic person.
There is evidence to suggest that food handlers who wear latex gloves may
contaminate the food with latex allergens and are themselves at risk of developing
latex allergy. It has been demonstrated that when a person with latex allergy eats
cross-contaminated food, it can cause an allergic reaction. There is also a possible risk
from latex adhesives used in food packaging. These are used as cold seal adhesives in
cases where hot seals would damage a product, such as ice cream. There are reports of
people reacting to these adhesives.
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CHAPTER 3
METHODOLOGY
Phr
Amount Needed
Ingredients
Dry Actual (g)
This formulation is a common formulation, which has been used as the basic latex
formulations of compounding ingredients.
6
Example of calculation to determine the actual weight of the ingredients.
Accelerators e.g. Mercaptobenzothiazoles (MBT)
Amount to be prepared = 500 g
Part by Weight
MBT 50
Dispersing Agent 1
Bentonite Clay 1
Soft Water 48
Total 100
Actual weight can be calculate by multiplying the Part By Weight value with the
factor
Example:
MBT
7
After that, the mixture will undergo a dispersion test where a drop of the
mixture is dropped into a cylindrical tube filled with water so that the dispersion of
the chemicals particle can be observed.
This test is crucial to determine whether the particle size of the chemical is
well milled. If the particle is dropping abruptly after it is tested in the tube, the milling
time will needed to be extend until the particle size is well balance. If the unbalanced
of particle size which consist in the chemicals are to be mix with the latex,
coagulation of the mixture will initiates and the result will be totally invalid. Worse,
the mixture will undergo a rapid curing phase where the mixture cannot be used to
produce any products anymore.
Table 1.2 showing the chemical ingredients that will be mix with the latex by the
NAME FORM
Potassium Oleate 10% aqueous solution
Potassium Hydroxide (KOH) 10% aqueous solution
Sulphur 50% dispersion
Zinc Oxide (ZnO) 50% dispersion
ZDEC 50% dispersion
MBT 50% dispersion
Table 2 Chemical Ingredients
All the chemical ingredients are prepared in 50% dispersion with the
exception of foaming agent, Potassium Oleate and Potassium Hydroxide (KOH).
Although the dispersion value is the same, the amount that we use is still base on the
recipe, which is shown in the table 2.4 below.
8
The recipe, which has been used, is shown in table 2.4
Dry/Actual Dry Actual Dry Actual Dry Actual Dry Actual Dry Actual
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3.3 Latex Testing
Before the latex can be mix with processing ingredients, it has to be tested to
measure the stability and to determine whether it is suitable to be used in
manufacturing products.
Total solid content can be defined as the percentage weight of latex which is
non-volatile at the given temperature in an open atmosphere and is therefore a
measurement of the total rubber and non-rubber solid in the latex itself.
TSC = M1 / M0 X 100
Where;
The result of duplicate determinations shall not differs by more than 0.2 unit
10
2. Dry Rubber Content – ISO 126 1972
The dry rubber content is the percentage weight of latex, which coagulable by acetic
acid under closely defined conditions. The difference between TSC and DRC
represents the soluble non-rubber solids content.
DRC = M1 / M0 X 100
Where;
The procedure is to be triplicate and the results should agree within 0.2% m/m DRC
Alkalinity refers to the total amount of alkali present in the latex and is often
expressed as the amount of added ammonia in the latex.
Calculation of alkalinity
Where:
F1 is the factor, 1.7 for hydrochloric acid and 3.4 for sulphuric acid.
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The result of the duplicate determination should not be differ more than 0.02
unit where the actual alkalinity is above 0.5 unit or shall not differ by more than 0.01
unit where the actual alkalinity is 0.5 unit or less.
The viscosity of latex can determine the performance of the latex during
processing. The viscosity of latex is variable and therefore is determined at 60.0 ±
0.1% total solid content at various rates of shear at 25ºC by using Brookfield
Viscometer. The viscosity in centipoises is calculated by multiplying the dial readings
by the various spindles.
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Viscosity Testing
element (the spindle) in latex by using a multiple speed transmission and spindle
chosen. In this testing for latex the suitable spindle is spindle no.2.
Procedure:
2. Then, remove bubble from the surface of the latex with a piece of filter paper.
4. Level the viscometer and lower the spindle into latex until the surface of the
latex is level with the groove marked on the spindle. The spindle should be
placed at the centre of the beaker. Check the level of the viscometer again.
6. Release clutch and allow dial to rotate until a steady reading is obtained.
The formula of calculating viscosity is based on the spindle number, which is used to
measure the viscosity of latex.
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Speed (rpm)
Spindle No.
6 30 60
1 10 2 1
2 50 10 5
3 200 40 20
In this experiment, the sample is prepared using two methods, which is called
curing and matured.
Curing
In curing, the latex is mixed in a fixed volume with all the compounding
ingredients such as accelerator, activator etc. The mixtures first need to be
homogeneously mixed and stirred for about 40 minutes. After that, the mixture is put
into water bath at 70ºC for about 2 hours. During this period, the mixtures still need to
be stir to avoid the presents of coagulum.
The process is continues by filtering the mixture to separate the coagulum
from the mixture. This process is crucial to make sure the mixture that will be pour
into casting mould is not mix with the coagulum because this will effect the result of
the experiment when testing the sample. After the sample has been poured into the
mould, the mixture is left in room temperature for about 3 days so that the water and
ammonia in the mixture can be completely vaporized.
After three days, the sample of the latex in a thin sheet with the thickness of
about 2cm finally produced. Calcium carbonate CaCO3 is put on both surface of the
sheet.
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Figure 3: Casting mould for dumbbells sample
Matured
In matured, the step is not that different compared to cured except that there is
no involvement of water bath. The sample is mixed with all of the compounding
ingredients and then is left in room temperature for approximately 14 days before it is
poured into the mould. The mixture in the beaker needs to be sealed with aluminium
foil to prevent the water and ammonia, which presents in the latex vaporized to the
atmosphere.
During the period, viscosity test is run on the mixture in 3 days interval to
determine the changes which may occur during the maturing period. After 14 days,
the mixture will then be poured into the mould and be left again for about 3 days to
get the rubber sheets before the dumbbells sample can be produce with the profile
cutter.
Sampling
After the sample has finally been produced, it will undergo cutting process
using the profile cutter to get the dumbbells sample. After the samples h
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Figure 4: Dumbbell sample
3.5 Testing
Tensile Testing
16
Tensile testing is a measurement of the ability of latex to withstand forces that
tend to pull it part and determine to what extent the latex stretches before it breaking.
Procedure:
• First the sample of latex cut into the dumbbell to easy used equipment testing.
• Position the test specimen vertically in the grips of the testing machine.
• Before put the sample on the grip need to measure width and thickness.
• Set the speed of testing at proper rate and start the machine.
observed.
calculated.
• The tensile modulus and elongation value are derived from the stress-strain
curve.
• Study characteristic features of the stress and strain cue for the respective
materials.
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CHAPTER 4
DATA SHEET
TSC = M1 / M0 X 100
Where;
The result of duplicate determinations shall not differs by more than 0.2 unit
Sample B
Mass before drying of sample B = 2.041g (M0)
Mass of dried portion after drying at 70ºC = 1.244g (M1)
Discussion
18
From this test, the result indicated that the latex, which will be use for
compounding, is suitable to be use because the percentage of the total solid content,
which presents in the latex, is ranges from about 60.8 to 61%.
DRC = M1 / M0 X 100
Where;
The procedure is to be triplicate and the results should agree within 0.2% m/m
DRC
Data Sheet
Sample A
Sample B
For dry rubber content, usually the result is ranges from 59-60±. The latex, which is
tested, has been used before so the content has been exposed to the atmosphere which
causing the result to be exceptionally low.
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ALKALINITY TEST
Result of titration
Volume of 1st titration = 18.3cm³
Average = 18.153cm³
VISCOSITY TEST
Result
1 600 - -
2 625 280 197.5
3 600 300 220
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4.2 TENSILE TESTING
RESULT
Machine parameters
Cured sample
Discussion
1st recipe
The table above shows the value of tensile strength and ultimate elongation of
the 1st recipe for cured sample. From all five samples, the 1st sample is the ones
produced the highest value of tensile strength and ultimate elongation; 7.4037 Mpa
and 3895.5 mm.
The 1st recipe did not include mercaptobenzothiazole (MBT) as the accelerator
because the 1st recipe is utilized as the control formulation. However this recipe is
mixed with 1 pphr of Zinc-Diethyldithiocarbamate (ZDEC).
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2nd recipe
Discussion
For the 2nd recipe, the amount of MBT and ZDEC which has been added to the
latex is the same; 0.5 pphr. The highest value, which is obtainable, is from the 4th
sample, which is 21.175 MPa for tensile strength and 3469.0 mm for the ultimate
elongation value.
3rd recipe
Discussion
For the 3rd sample, from all five sample that have been tested, the 3rd sample
has produced the highest value of tensile strength and ultimate elongation of about
23.664 MPa and 3936.0 mm. For this compounding recipe, the amount of MBT and
ZDEC used is the same, which is 0.25 pphr.
Matured Sample
1st recipe
Discussion
For matured sample, the table above showing the value of tensile strength and
ultimate elongation for the 1st recipe which contains just ZDEC as its accelerator
(control recipe).
From all five samples, the 1st sample is the one, which produce the highest
value of tensile strength and ultimate elongation; 18911000 N/m², 3636.6 mm.
2nd recipe
Discussion
The 2nd recipe is mixed with 0.5 pphr of MBT and 0.5 pphr ZDEC as the
accelerator. The highest value tensile strength is obtainable is from the 4 th sample,
10437000 N/m². However, the highest value of ultimate elongation is produced by the
1st formulation; 2863.3 mm. This occurrence maybe because of the sample texture is
altered during the process of cutting the sample due to human error.
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3rd recipe
Discussion
For the 3rd recipe, the 5th sample showing the highest value of tensile strength
including the ultimate elongation; 15765000 N/m², 3128.6 mm. The amount of
accelerator used in this recipe is the same as the 3rd recipe for the cured sample; 0.25
for MBT and 0.25 for ZDEC.
Conclusion
Cured Sample
From all 15 samples from three different recipes, which contain different
combination of accelerator amount, it is clear that the 3rd recipe has the ability of
producing the greatest mechanical properties exceeding the other two recipes. For the
1st recipe, the average value of tensile strength and ultimate elongation is 6.775 MPa
and 3405.9 mm for the ultimate elongation.
For the 2nd recipe, the value of tensile strength is 18.827 MPa and 3691.5 mm
for the ultimate elongation. Last but not least, the value of tensile strength and
ultimate elongation of the 3rd sample is 21.267 MPa and 3691.5 mm. From all three
recipes, it is proven that the 3rd recipe has the greatest mechanical properties in which
the amount of chemicals especially accelerator which is used in the compounding
ingredients is relatively low, 0.25:0.25 pphr.
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20
Stress (MPa)
15
10
5
0
-5 0 500 1000 1500 2000 2500 3000 3500 4000
Elongation (mm)
23
The graph above is produced base on the results from testing the samples,
which is from three different recipes. The graph is produced with the data which
having the highest value of tensile strength and elongation. For this graph, it shows
the properties of cured sample from the three recipes. It can be seen that from all three
recipes, the 3rd recipe has the greatest mechanical properties in terms of its tensile
strength and ultimate elongation.
Conclusion
Matured Sample
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15
Stress (MPa)
10
0
0 500 1000 1500 2000 2500 3000 3500
Ultimate Elongation (mm)
For all the matured samples from three recipes that have been tested by using
the Lloyd tensile tester, it can be seen that there are a little differences regarding the
tensile strength and ultimate elongation values between the 1st and the 3rd recipe. For
the 1st recipe, the average value of ultimate elongation is 3405.9 mm, which is bigger
than the value produced by the 3rd, which is 3035.8 mm. However the value of tensile
strength of the 3rd recipe exceeded the value of the 1st formulation, which is 13663200
N/m² for the 3rd and 11572200 N/m².
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4.3 SAMPLE FAILURES
In this experiment, there are total of 5 compounding recipes, which have been
formulated. These entire 5 recipes differs from one and another in terms of their ratio
or the amount of the accelerator which is mixed with the latex along with other
chemicals such as activator, dispersion agent etc. During the experiment of producing
the cured and matured sample, the 4th and 5th recipe failed and the mixture cannot be
use to produce samples because the mixture abruptly coagulates during the
experiment.
Such occurrences maybe because the amount of accelerator, which is mixed in
the latex is too high. For the 4th recipe, the amount of MBT and ZDEC used is both
relatively high; 2 pphr for both of the chemicals. This amount of accelerator used
affected the mixing process of the latex because it has accelerated the cross-linking
process at a very fast rate. This makes the mixture to harden and coagulum is
produced and directly damaging the compound.
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Figure 9: Failed Sample During Casting Process
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Comparison
25
20
Stress (MPa)
15
10
5
0
0 500 1000 1500 2000 2500 3000 3500 4000
Ultimate Elongation (mm)
Cured Matured
From all three recipes, only the 1st and the 3rd recipe manage to produce the
greatest results, which also proved its properties. So referring to the graph above, it
shows the comparison in terms of tensile strength and ultimate elongation for both
cured and matured sample. Looking at the graph, it is proven that producing samples
by cured method is much more efficient because it has better properties compared to
matured. Plus, by using cured method to produce sample, it is faster and this is
because there is no need to leave the mixture up to 2 weeks just for the mixture to
completely vulcanize.
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CHAPTER 5
RECCOMENDATION
From these project, the results conclude that MBT and ZDEC (accelerator)
which is used in the production of latex gloves need to be include in order to produce
products with a good mechanical properties which is crucial to act as a barrier of
protection both for the health care worker and the patient to guard against contact with
blood, other body fluids, and microorganisms.
The used of MBT need to be put a very serious consideration because the
higher the amount of MBT, which is added during compounding, the higher the risk
for the allergic reaction to occurs. MBT can be considered as the agent, which will
activate the reaction, in which will produce the protein, which is the cause of the
allergic reaction to occur. Despite of the allergic reaction that related to the amount,
which is used in compounding, from the experiment, the excessive usage of MBT
could also reduced the properties of the product.
This is because, if the amount is to high, the mixture will tend to produce
coagulum and will directly affect the process of stirring the mixture because it will
harden. This is because, the higher the amount of accelerator added to the latex, the
process of cross-linking will be much higher in terms of its rate of reaction. If the
reaction happens in such a very fast rate, premature vulcanization will happen.
REFERENCE
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APPENDIX A
1. LATEX TESTING
TSC = M1 / M0 X 100
Where;
M0 is the mass in gram of the test portions
M1 is the mass in grams of the dried sheet
The result of duplicate determinations shall not differs by more than 0.2 unit
Data Sheet
Before Drying
After Drying
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1.228/2.02 X 100 = 60.79%
APPENDIX B
Sample B
Mass before drying of sample B = 2.041g (M0)
Mass of dried portion after drying at 70ºC = 1.244g (M1)
DRC = M1 / M0 X 100
Where;
The procedure is to be triplicate and the results should agree within 0.2% m/m DRC
Data Sheet
Sample A
Mass of Petri dish A = 93.51g
Mass of Petri dish A + latex = 103.63g
Before drying
Mass of latex A = 10.119g
After Drying
Mass of latex A = 6.008g (M1)
Dry Rubber Content = M1 / M0 X 100
6.008/10.119 X 100 = 59.37%
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APPENDIX C
Sample B
Mass of Petri dish B = 93.92g
Mass of Petri dish B + latex = 104.04g
Before drying
Mass of latex B = 10.118g
After Drying
Mass of latex B = 6.006g (M)
For dry rubber content, usually the result is ranges from 59-60±. The latex, which is
tested, has been used before so the content has been exposed to the atmosphere which
causing the result to be exceptionally low.
Data Sheet
Sample A
After pouring
Weight of weighing bottle = 42.110g
Weight of latex used = 46.566-42.110
= 4.456gSample B
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APPENDIX D
Sample B
After pouring
Result of titration
Volume of 1st titration = 18.3cm³
Average = 18.153cm³
2nd titration
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APPENDIX E
1.4 TENSILE TESTING
RESULT
Machine parameters
Cured sample
1st recipe
2nd recipe
Sample No Tensile Strength (MPa) Ultimate Elongation
1 18.644 3301.9
2 20.863 3266.8
3 17.358 3267.9
4 21.175 3469.0
5 16.093 3214.9
Average 18.827 3304.1
APPENDIX F
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3rd recipe
Matured Sample
1st recipe
2nd recipe
Sample No Tensile Strength (N/m²) Ultimate Elongation
1 10216000 2863.3
2 9134500 2704.0
3 7325500 2314.2
4 10437000 2703.5
5 9703000 2549.6
Average 9363200 2626.9
APPENDIX G
3rd recipe
Sample No Tensile Strength (N/m²) Ultimate Elongation
1 14224000 3106.9
34
2 13477000 2977.6
3 12466000 2872.2
4 12384000 3093.8
5 15765000 3128.6
Average 13663200 3035.8
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APPENDIX H
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37
38
39
40
41
42
43
44
45
46
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APPPENDIX I
Brookfield
Viscometer
Casting Mould
48
Cured Sample in Water Bath
Mechanical Stirrer
49
Pebble Mill
50
Lloyd Tensile Tester
51