Hitachi High-Speed

Amino Acid Analyzer

CAUTION: For proper operation, follow the instruction manual when using the instrument.
Specifications in this catalog are subject to change with or without notice, as Hitachi High-Technologies Corporation continues to develop the latest
technologies and products for our customers.

For further information, please contact

your nearest sales representative.
Tokyo, Japan
http://www.hitachi-hitec.com
24-14 Nishi-Shimbashi 1-chome, Minato-ku, Tokyo, 105-8717, Japan
Tel: +81-3-3504-7111 Fax: +81-3-3504-7123

Printed in Japan(H) HTB–E028 2005.9

 EZChrom Elite Software for Easy Operation
The L-8900 is controlled by EZChrom EliteTM
chromatography data system, which supports
chromatography systems from various manufacturers
(the software has over 30,000 users worldwide).

Analyzer Status Screen (L-8900 Tab)

You can monitor and perform manual control while viewing the status data

Analyzer Status Screen

Gray background: OFF

Yellow background: ON

Maintenance buttons

Hitachi's New Amino Acid Analyzer, Model L-

Manual control buttons
8900, is the culmination of the technology and
expertise earned from our extensive experience
in manufacturing amino acid analyzers during
Analyzer Status Screen (Option Tab)
the past 40 years. You can monitor and perform manual control while viewing the status data.

High Sensitivity Realized by the Reaction Column System Reaction column

The“reaction column”system＊1 was first introduced in Model Lamp energy icon

B 3 pmol
L-8800, the predecessor of the L-8900 launched in 1997. With (Asp., S/N: 2, standard
A
the reaction column system, the highest detection limit ever of 3 method of analysis)
pmol (Asp., S/N: 2, standard method of analysis) has been
Ser

achieved, far exceeding the 10 pmol limit achievable with the

conventional“reaction coil”system (Asp., S/N: 3, standard
Asp
Thr

method of analysis). The L-8800 also uses the rection column Pump purge button
system to provide the highest sensitivity among the amino acid
Linear Velocity
analyzers using the ninhydrin reaction method.

＊1 To achieve enhanced sensitivity, the reaction column is filled with special particles, which
B N2 gas automatic purge button
are used to suppress peak dispersion for better separation. In addition, the loading
pressure has been reduced to improve the reaction efficiency. Automatic operation of solenoid valve
A
4 6 (new function)

1 2
 DATA

Improved Hardware - New applications made ● PH (Protein Hydrolysate)

High-speed standard analysis, 20 minutes
possible by the new and improved hardware Column size: 4.6 mm ID x 40 mm

Cys
Resin: #2622

Ser

Lys
Val
Thr

His
Asp

Glu

Met
Ala
･N2 gas automatic purge ＊2

Phe
Tyr

NH3
･Leak sensor

Gly

Ile

Arg
･Degasser

Leu
･Accommodates up to 6 buffers ＊3
･Max. temp: 140 ° C (Reaction unit)＊4

Trp
･Max. temp: 80 ° C (Column oven)＊5

Pro
･New and improved pump
･New, direct injection autosampler ＊6
0 2 4 6 8 10 12 14 16 18 20
Retention time (min)
＊2: Automatic operation of solenoid valve
＊3,＊4 &＊5: Together the new hardware features support new
applications.
＊6: The new autosampler reduces the impact on the injection
selector valve produced when the sample is injected to about
1/7 of that with the existing autosamplers. As a result, it
provides improved accuracy in determination of the peak that ● PH (Protein Hydrolysate)
appears within 5 minutes after the start of separation. Standard analysis, 30 minutes
Column size: ?.6 mmID x 60 mm
Resin: #2622

Cys

Lys
Val
Ser
Thr
Asp

Glu

His
Met

Tyr
Phe

NH3
Gly

Arg
Ile

Leu
Ala

Trp
0 10 20 30
Retention time (min)

● High-speed physiological
35
fluid analysis, 120minutes

HCys
Asp
Column size: 4.6 mmID x 60 mm

ASA-Anhy1
30

Thr
Resin: #2622

P-Ser

Orn
GluNH2
Ser

Lys
Impact reduced to approx. 1/7 Data acquired using an existing autosampler

P-Ser
25

Gly

AEC
Ala
Cit

Cysthi
Signal strength (mv)

1Mehis
20

Glu

Hylys
Phe

g-ABA

His
Hylys

3Mehis
Met
Val

Cys
Urea

Arg
15
PEA

AspNH2

a-ABA

ASA
Sar

Leu
IIe

EOHNH2
HCit
0.8mV 10

NH3
MetSOX

a-AAA

b-AiBA
Tyr

Car
b-Ala
Cys-HCys
HCysH

Saccha

ALevA
AIIo-IIe

Trp

Ans
Pipeco
5
Hypro

Pro

0 20 40 60 80 100 120 140

Retention time (min)

3 4
 DATA SPECIFICATIONS

● Biological sample partial ■ Standard Specifications

analysis, 26 minutes Performance (results of measuring 5 samples by protein hydrolyzate assay (standard) method)
Analysis time Approx. 30 minutes (net)
Maple syrup urine disease Column size: 4.6 mmID x 60 mm
30 Reproducibility of peak retention time CV0.3% (Arg), 0.5% (Ala)
Resin: #2622
Hypermethio- Reproducibility of peak area CV1.0%(Gly, His)

Val
ninemia

Acetyl-Lys
25 Detection limit 3 pmol (S/N = 2, Asp)
Cystathionin-
uria Phenylketonuria Analyzer
Column 4.6 mm ID ' 60 mm L ※1

Met
Signal strength (mv)

20

AIIo-IIe
Packed with Hitachi custom ion exchange resin

Leu
IIe

Phe
2
15 Pump Delivery pressure; 0 to 20 MPa (0 to 306 kgf/cm )

Cysthi
● Biological sample partial Flow rate range; 0.000 to 0.999 mL/min

Tyr
10 analysis, 26 minutes Autosampler Injection methods; Direct injection method
Sample vial capacity; 1500 µL
Column size: 4.6 mmID x 60 mm
No. of vials accommodated; 120 (80 when optional cooler installed)
5
Resin: #2622
Sample injection volume; 0.1 to 100 µL
Column oven System; Peltier
0
Temperature setting range; 20 to 85 °
C (in 1 °
C steps)
0 2 4 6 8 10 12 14 16 18 20 22 24 26
Reaction unit System; Electronic heating (reaction column)
Retention time (min)
Temperature setting; 50 to 140 °
C (in 1 °
C steps)
Photometer Spectrophotometer; Aplanatic concave diffraction grating
Wavelength; 570 nm, 440 nm
EZChrom Elite for HITACHI Amino Acid Analyzer
※2
CPU 32 bit OS, Windows XP ®
● Biological sample partial CRT 17 inch color display
analysis, 10 minutes Dimensions (excluding PC) 900(W) ' 650(D) ' 1050(H) mm
Weight (excluding PC) Approx. 250 kg
Column size: 4.6 mmID x 60 mm
Operating temperature range 15 to 35 °
C
Resin: #2622
Power supply 100 ∼115 V AC / 220 ∼240 V AC, 800 VA and over, w/in 50/60 Hz ± 0.5 Hz
N2 gas N2 gas source must be prepared.
Tyr

※1 Inch (unify) thread applicable

Phe
※2 Windows is a registered trademark of Microsoft Corp. in the U.S. and other countries.

■ Set Layout

100∼
200 or greater 900 300 700

400 or greater
Power supply N
2
& grounding gas
0 1 2 3 4 5 6 7 8 9 10 2m 5m
Retention time (min)

650
PC table
L-8900 or
Main unit PC rack

● High-speed biological sample

1,000 or greater
This space is unnecessary
Cys

analysis, 70 minutes if PC is mounted on top of main unit.

Val

Column size: 4.6 mmID x 80 mm

Resin: #2622
Unit:mm
Met
P-Ser

Orn
Gly

g-ABA

Lys
Ala
Cit
Asp

Hylys
Cysthi
Tau

Phe
GluNH2

Leu Ile
Thr

Glu
Ser

3Mehis
b-AiBA

1Mehis

Arg
b-Ala

His
a-ABA

Tyr
Urea PEA

EOHNH2
AspNH2

Trp
a-AAA
Sar

Car
NH3

Ans

0 5 10 15 20 25 30 35 40 45 50 55 60 65 70
Retention time (min)

5 6