7.75929.0002-xxxxxxxxxx msp.

June 2016

1.14832.0001 • water
Samples containing more than 2.5 mg/l Zn must be diluted with distilled
prior to digestion.

Spectroquant® • The pH must be within the range 4 - 10.

Zinc Test Zn Adjust, if necessary, with sodium hydroxide solution or sulfuric acid.
• Filter turbid samples.
7. Procedure
1. Method
Pretreated sample 5.0 ml Pipette into a test tube.
In alkaline solution zinc ions react with a pyridylazonaphthol derivative to form a (10 - 35 °C)
red complex. This is extracted and then determined photometrically. Reagent Zn-1 5 drops 1)
Add and mix.
The pH must be above 12.
2. Measuring range and number of determinations Check with MColorpHastTM universal indicator
strips. Adjust the pH, if necessary, with sodium
hydroxide solution.
Number of
Cell Measuring range Reagent Zn-2 2 drops 1) Add carefully (potassium cyanide!), close
determinations
the test tube tightly, and mix.
10 mm 0.05 - 2.50 mg/l Zn 100 Reagent Zn-3 5 drops 1) Add, close the tube tightly, and mix.
Reagent Zn-4 3 drops 1) Add carefully (formaldehyde!), close the
For programming data for selected photometers / spectrophotometers see tube tightly, and mix.
www.service-test-kits.com.
Leave to stand for 3 min (reaction time A).
3. Applications Reagent Zn-5 1 level grey Add, close the tube tightly, and shake vigor-
This test measures only zinc ions. Samples must be decomposed by digestion microspoon ously until the reagent is completely dis-
(in the cap of solved.
before undissolved or complex-bound zinc can be measured (see section 6). the Zn-5 bottle)
Sample material: Extracting agent 2) 5.0 ml Add with pipette and close the tube tightly.
Wastewater, especially from electroplating and metal-processing factories
Groundwater and surface water, drinking water Shake the tube vigorously for 30 seconds and subsequently leave to stand for 2 min
This test is not suited for seawater. (reaction time B).
Withdraw the clear upper (organic) phase with the suction pipette and fill into a 10-mm
cell.
4. Influence of foreign substances Leave to stand for 3 min (reaction time C), then measure the sample in the photometer.
This was checked in solutions containing 2 and 0 mg/l Zn. The determination is 1)
not yet interfered with up to the concentrations of foreign substances given in Hold the bottle vertically while adding the reagent!
2)
the table. see section 5, “Also required”

Concentrations of foreign substances in mg/l or % Notes on the measurement:

Ag+
Al3+
10
1000
Cu2+
F-
10
1000
NO2-
Pb2+
1000
100
EDTA
Hydrazine
0.1
1000
• Certain photometers may require a blank (preparation as per measure-
ment sample, but with distilled water instead of sample).
Ca2+ 100 Fe3+ 5 PO43- 1000 Surfactants 1) 10 The blank is yellow in color.
Cd2+ 10 Hg2+ 10 S2- 1 Na-acetate 10 %
CN- 1000 Mg2+ 10 SiO32- 100 NaCl 5% • Wipe,
For photometric measurement the cells must be clean.
if necessary, with a clean dry cloth.
Co2+ 10 Mn2+ 1 Sn2+ 100 NaNO3 15 %
Cr3+
Cr2O72-
10
1000
NH4+
Ni2+
1000
5
SO32- 1000 Na2SO4 15 %
• measured (upper
Measurement of turbid solutions yields false-high readings. The extract to be
phase) must be clear. If this is not the case, leave the test
1)
tested with nonionic, cationic, and anionic surfactants tube to stand for longer than 2 min prior to sucking off the upper layer.

5. Reagents and auxiliaries

• after
The color of the measurement solution remains stable for at least 60 min
the end of the reaction time C.

Please note the warnings on the packaging materials! Caution! Reagent

Zn-2 contains potassium cyanide! Reagent Zn-4 contains formaldehyde!
8. Analytical quality assurance
recommended before each measurement series
The test reagents are stable up to the date stated on the pack when stored To check the photometric measurement system (test reagents, measurement
closed at +15 to +25 °C. device, handling) and the mode of working, Spectroquant® CombiCheck 100
can be used. Besides a standard solution with 0.75 mg/l Zn, this article also
Package contents:
contains an addition solution for determining sample-dependent interferences
1 bottle of reagent Zn-1
(matrix effects).
1 bottle of reagent Zn-2
Additional notes see under www.qa-test-kits.com.
1 bottle of reagent Zn-3
1 bottle of reagent Zn-4 Characteristic quality data:
1 bottle of reagent Zn-5 In the production control, the following data were determined in accordance with
2 test tubes with screw caps ISO 8466-1 and DIN 38402 A51:
1 suction pipette
1 AutoSelector Standard deviation of the method + 0.030
(mg/l Zn)
Also required:
Coefficient of variation of the method + 2.3
Extracting agent, sufficient for approx. 200 determinations: (%)
Isobutyl methyl ketone for analysis EMSURE®, Cat. No. 106146
Confidence interval + 0.06
(mg/l Zn)
Other reagents and accessories:
Spectroquant® Crack Set 10C, Cat. No. 114688 Number of lots 24
+ thermoreactor
or Characteristic data of the procedure:
Spectroquant® Crack Set 10, Cat. No. 114687
Sensitivity: 0.01
+ empty cells 16 mm with screw caps (25 pcs), Cat. No. 114724 Absorbance 0.010 A
+ thermoreactor corresponds to (mg/l Zn)
MColorpHastTM Universal indicator strips pH 0 - 14, Cat. No. 109535 Accuracy of a measurement value max. ± 0.07
Sodium hydroxide solution 1 mol/l Titripur®, Cat. No. 109137 (mg/l Zn)
Sulfuric acid 0.5 mol/l Titripur®, Cat. No. 109072
Spectroquant® CombiCheck 100, Cat. No. 118701 For quality and batch certificates for Spectroquant® test kits see the website.
Hydrochloric acid 25 % for analysis EMSURE®, Cat. No. 100316
Pipette for a pipetting volume of 5.0 ml 9. Notes
Flat-bottomed tubes for Aquamerck® tests (12 pcs), Cat. No. 114902
Rectangular cells 10 mm (2 pcs), Cat. No. 114946 • Reclose the reagent bottles immediately after use.
• The contents of the test tubes and of the cells as well as the test rea-
gents must not be run off with the wastewater!
6. Preparation Information on disposal can be obtained at www.disposal-test-kits.com.
• Thoroughly rinse the test tubes and the cells with hydrochloric acid and
distilled water prior to each determination.
• Analyze immediately after sampling. Merck KGaA, 64271 Darmstadt, Germany,
• Undissolved or complex-bound zinc can be determined after pretreatment of
the sample using one of the Spectroquant Crack Sets. ®
Tel. +49(0)6151 72-2440
www.analytical-test-kits.com
EMD Millipore Corporation, 290 Concord Road,
Billerica, MA 01821, USA, Tel. +1-978-715-4321