Hermogenes, NC

Huna, JV
Judan, MB

Group 2
LAB 1: Effect of pH on the Partition Coefficient of a Weak Acid
A. Preparation of UV absorbance standard curve

Graph of standard curve

Performed by

Checked by
Concentration of
sodium salicylate Absorbance at 525 nm
standard
solution (mM)
1 2 3 Mean
0.5
1.0
1.5
2.5

Computation for standard curve

Performed by: Checked by:

 B. Determination of Apparent Partition Coefficient

Performed by

Performed by

Performed by
Checked by

Checked by

Checked by
Apparent
Buffer Actual pH Absorbance partition
solution coefficient
1 2 3 Mean 1 2 3 Mean
pH 1.2
HCl
pH 2.9
acid
phthalate

Computation of Apparent Partition Coefficient

Performed by: Checked by:

C. Measurement of True Partition Coefficient

Volume

Performed by

Performed by

Performed by
Checked by

Checked by

Checked by
of 0.2N True
sodium Absorbance before extraction Absorbance after extraction partition
salicylate coefficient
(mL)
1 2 3 Mean 1 2 3 Mean
10.0

Computation of True Partition Coefficient

Performed by: Checked by: