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Service Manual
© 2007-2008 Shenzhen Mindray Bio-medical Electronics Co., Ltd. All rights
Reserved.
For this Service Manual, the issued Date is 2008-07 (Version: 1.1).
All information contained in this manual is believed to be correct. Mindray shall not be
liable for errors contained herein nor for incidental or consequential damages in
connection with the furnishing, performance, or use of this manual.
Mindray is responsible for safety, reliability and performance of this product only in
the condition that:
WARNING:
It is important for the hospital or organization that employs this
equipment to carry out a reasonable service/maintenance plan.
Neglect of this may result in machine breakdown or injury of human
health.
i
Warranty
THIS WARRANTY IS EXCLUSIVE AND IS IN LIEU OF ALL OTHER WARRANTIES,
EXPRESSED OR IMPLIED, INCLUDING WARRANTIES OF MERCHANTABILITY
OR FITNESS FOR ANY PARTICULAR PURPOSE.
Exemptions
Mindray's obligation or liability under this warranty does not include any
transportation or other charges or liability for direct, indirect or consequential
damages or delay resulting from the improper use or application of the product or the
use of parts or accessories not approved by Mindray or repairs by people other than
Mindray authorized personnel.
Return Policy
Return Procedure
In the event that it becomes necessary to return this product or part of this product to
Mindray, the following procedure should be followed:
Company Contact
Manufacturer: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.
Address: Mindray Building, Keji 12th Road South, Hi-tech Industrial Park,
Nanshan, ShenZhen518057, P.R.China
Tel: +86 755 26582479 26582888
Fax: +86 755 26582934 26582500
ii
Foreword
Who Should Read This Manual
This manual is geared for service personnel authorized by Mindray.
Bold and Italic font indicates text displayed on the screen, such as Sample
Request.
Safety Symbols
This chart explains the symbols used in this manual.
Serial Number
Date of Manufacture
Foreword 1
Manufacturer
ON (Main Power)
ON (Power)
OFF (Power)
HIGH CONC.
High-concentration waste
WASTE
HIGH CONC.
High-concentration waste sensor
WASTE SENSOR
LOW CONC.
High-pressure low-concentration waste
WASTE 1
2 Foreword
LOW CONC.
Normal-pressure low-concentration waste
WASTE 2
DEIONIZED
Deionized water
WATER
Model:
Product model
BS-400/BS-420
Graphics
All graphics, including screens and printout, are for illustration purposes only and
must not be used for any other purpose.
EC Representative
Name: Shanghai International Holding Corp. GmbH (Europe)
Address: Eiffestraβe 80, 20537 Hamburg Germany
Phone: 0049-40-2513175
Fax: 0049-40-255726
Foreword 3
Safety Precautions
Observe the following safety precautions when using the BS-400/BS-420 Chemistry
Analyzer. Ignoring any of these safety precautions may lead to personal injury or
equipment damage.
WARNING
If the system is used in a manner not specified by Mindray, the
protection provided by the system may be impaired.
WARNING
When the Main Power is on, users must not open the rear cover or side
cover.
Spillage of reagent or sample on the analyzer may cause equipment
failure and even electric shock. Do not place sample and reagent on the
analyzer. In case of spillage, switch off the power immediately, remove
the spillage.
WARNING
Do not touch such moving parts as sample probe, reagent probe, mixer
and wash probe, when the system is in operation.
Do not touch the sample probe or mixer while the system is in operation.
Make sure the reagent disk cover is properly installed.
WARNING
Light sent by the photometer lamp may hurt your eyes. Do not stare
into the lamp when the system is in operation.
If you want to replace the photometer lamp, first switch off the Main
Power and then wait at least 15 minutes for the lamp to cool down
before touching it. Do not touch the lamp before it cools down, or you
may get burned.
4 Foreword
Preventing Laser Radiation
Please observe the following instructions to prevent personal injury caused by laser
radiation.
CAUTION
Light sent by the bar code reader may hurt your eyes. Do not stare into
the laser beam from the bar code reader.
Preventing Infection
Please observe the following instructions to protect against the biohazardous
infection.
BIOHAZARD
Inappropriately handling samples, controls and calibrators may lead to
biohazardous infection. Do not touch the sample, mixture or waste with
your hands. Wear gloves and lab coat and, if necessary, goggles.
In case your skin contacts the sample, control or calibrator, follow
standard laboratory safety procedure and consult a doctor.
WARNING
Reagents, concentrated wash solution and enhanced wash solution
are corrosive to human skins. Exercise caution when using the
reagents, concentrated wash solution and enhanced wash solution. In
case your skin or clothes contact the reagents or wash solution, wash
them off with soap and clean water. In case the reagents or wash
solution spill into your eyes, rinse them with much water and consult an
oculist.
BIOHAZARD
Some substances in reagent, control, enhanced wash solution and
waste are subject to regulations of contamination and disposal. Dispose
of them in accordance with your local or national guidelines for
biohazard waste disposal and consult the manufacturer or distributor of
the reagents for details.
Wear gloves and lab coat and, if necessary, goggles.
Foreword 5
WARNING
Materials of the analyzer are subject to contamination regulations.
Dispose of the waste analyzer in accordance with your local or national
guidelines for waste disposal.
WARNING
Ethanol is flammable substance. Please exercise caution while using the
ethanol.
6 Foreword
Precautions on Use
To use the BS-400/BS-420 Chemistry Analyzer safely and efficiently, please pay
much attention to the following operation notes.
Intended Use
WARNING
The BS-400/BS-420 is a fully-automated and computer-controlled
chemistry analyzer designed for in vitro quantitative determination of
clinical chemistries in serum, plasma, urine and CSF samples. Please
consult Mindray first if you want to use the system for other purposes.
To draw a clinical conclusion, please also refer to the patient’s clinical
symptoms and other test results.
Operator
WARNING
The BS-400/BS-420 is to be operated only by clinical professionals,
doctors or laboratory experimenters trained by Mindray or
Mindray-authorized distributors.
Environment
CAUTION
Please install and operate the system in an environment specified by
this manual. Installing and operating the system in other environment
may lead to unreliable results and even equipment damage.
Foreword 7
Preventing Interference by Electromagnetic Noise
CAUTION
Electromagnetic noise may interfere with operations of the system. Do
not install devices generating excessive electromagnetic noise around
the system. Do not use such devices as mobile phones or radio
transmitters in the room housing the system. Do not use other CRT
displays around the system.
Do not use other medical instruments around the system that may
generate electromagnetic noise to interfere with their operations.
Do not use this device in close proximity to sources of strong
electromagnetic radiation (e.g. mobile phones or radio transmitters), as
these may interfere with the proper operation.
The electromagnetic environment should be evaluated prior to operation
of the device.
This device has been designed and tested to CISPR 11 Class A, and in
a domestic environment may cause radio interference, in which case,
you may need to take measures to mitigate the interference.
CAUTION
Operate the system strictly as instructed by this manual. Inappropriate
use of the system may lead to unreliable test results or even equipment
damage or personal injury.
Before using the system for the first time, run the calibration program
and QC program to make sure the system is in proper status.
Be sure to run the QC program every time you use the system,
otherwise the result may be unreliable.
Do not open the covers of the sample disk and reagent disk when the
system is in operation.
The RS-232 port on the analyzing unit is to be used for connection with
the operation unit only. Do not use it for other connections. Only use the
supplied cable for the connection.
The operation unit is a personal computer with the BS-400/BS-420
operating software installed. Installing other software or hardware on this
computer may interfere with the system operation. Do not run other
software when the system is working.
Computer virus may destroy the operating software or test data. Do not
use this computer for other purposes or connect it to the Internet.
Do not touch the display, mouse or keyboard with wet hands or hands
with chemicals.
Do not place the Main Power to ON again within 10 seconds since
placing it to OFF; otherwise the system may enter protection status. If it
does so, switch off the Main Power and switch it on again.
8 Foreword
Maintaining the System
CAUTION
Maintain the system strictly as instructed by this manual. Inappropriate
maintenance may lead to unreliable results, or even equipment damage
and personal injury.
To wipe off dust from the system surface, use a soft, clean and wet (not
too wet) cloth, soaked with mild soap solution if necessary, to clean the
surface. Do not use such organic solvents as ethanol for cleaning. After
cleaning, wipe the surface with dry cloth.
Switch off all the powers and unplug the power cord before cleaning.
Take necessary measures to prevent water ingression into the system,
otherwise it may lead to equipment damage or personal injury.
Replacement of such major parts as lamp, photometer, sample probe,
reagent probe, mixer and syringe plunger assembly must be followed by
a calibration.
Samples
CAUTION
Use samples that are completely free of insoluble substances like fibrin,
or suspended matter; otherwise the probe may be blocked.
Medicines, anticoagulants or preservative in the samples may lead to
unreliable results.
Hemolysis, icterus or lipemia in the samples may lead to unreliable test
results, so a sample blank is recommended.
Store the samples properly. Improper storage may change the
compositions of the samples and lead to unreliable results.
Sample volatilization may lead to unreliable results. Do not leave the
sample open for a long period.
Some samples may not be analyzed on the BS-400/BS-420 based on
parameters the reagents claim capable of testing. Consult the reagent
manufacturer or distributor for details.
Certain samples need to be processed before being analyzed by the
system. Consult the reagent manufacturer or distributor for details.
The system has specific requirements on the sample volume. Refer to
this manual for details.
Load the sample to correct position on the sample disk before the
analysis begins; otherwise you will not obtain correct results.
CAUTION
To define such parameters as sample volume, reagent volume and
wavelength, follow the instructions in this manual and the package insert
of the reagents.
Foreword 9
Reagents, Calibrators and Controls
CAUTION
Use appropriate reagents, calibrators and controls on the system.
Select appropriate reagents according to performance characteristic of
the system. Consult the reagent suppliers, Mindray or
Mindray-authorized distributor for details, if you are not sure about your
reagent choice.
Store and use reagents, calibrators and controls strictly as instructed by
the suppliers. Otherwise, you may not obtain reliable results or best
performance of the system.
Improper storage of reagents, calibrators and controls may lead to
unreliable results and bad performance of the system even in validity
period.
Perform a calibration after changing reagents. Otherwise, you may not
obtain reliable results.
Contamination caused by carryover among reagents may lead to
unreliable test results. Consult the reagent manufacturer or distributor
for details.
Backing up Data
NOTE
The system can automatically store data to the built-in hard disk of the
PC. However, data loss is still possible due to mis-deletion or physical
damage of the hard disk. Mindray recommends you to regularly back up
the data to portable storage device.
NOTE
Refer to the operation manuals of computer and printer for details.
External Equipment
WARNING
External equipment connected to the system, such as PC and printer,
shall be consistent with IEC 60950, EN 60950, EN55022 (Class B) and
EN55024.
10 Foreword
Contents
Foreword ........................................................................................................... 1
Who Should Read This Manual.......................................................................................... 1
What Can You Find in This Manual .................................................................................... 1
Conventions Used in This Manual...................................................................................... 1
Safety Precautions ............................................................................................................. 4
Precautions on Use ............................................................................................................ 7
Contents............................................................................................................. I
1 System Description .............................................................................. 1-1
1.1 Overview............................................................................................................... 1-1
1.2 System Components ............................................................................................ 1-2
1.3 Functions .............................................................................................................. 1-3
2 System Performance and Workflow ................................................... 2-1
2.1 Technical Specifications........................................................................................ 2-1
2.1.1 General .................................................................................................... 2-1
2.1.2 Specifications for Sample System ........................................................... 2-2
2.1.3 Specifications for Reagent System.......................................................... 2-3
2.1.4 Specifications of Reaction System .......................................................... 2-5
2.1.5 Specifications of Operation...................................................................... 2-6
2.1.6 Installation Requirements ........................................................................ 2-6
2.1.7 Optional Modules..................................................................................... 2-7
2.2 Timing Principle .................................................................................................... 2-7
2.2.1 Overview.................................................................................................. 2-7
2.2.2 Timing ...................................................................................................... 2-7
2.2.3 Test Workflow......................................................................................... 2-10
2.2.4 Measuring Points ................................................................................... 2-11
3 Installation Procedures ........................................................................ 3-1
3.1 Environmental Requirements ............................................................................... 3-1
3.2 Installation Requirements ..................................................................................... 3-2
3.2.1 Space and Accessibility Requirements.................................................... 3-2
3.2.2 Power Requirements ............................................................................... 3-2
3.2.3 Water Supply and Drainage Requirements ............................................. 3-2
3.2.4 Connecting Water Supply and Drain Facilities ........................................ 3-4
3.2.5 Connecting Water Unit(Optional)............................................................. 3-5
3.3 Installation Procedures ......................................................................................... 3-8
3.3.1 Checking before Intallation ...................................................................... 3-8
3.3.2 Unpacking ................................................................................................ 3-8
3.3.3 Install Drawer and Air Pump .................................................................. 3-12
3.3.4 Install the Probe and the Mixing Bar...................................................... 3-14
3.3.5 Connect the System .............................................................................. 3-19
3.3.6 Run debug software............................................................................... 3-19
3.3.7 Operation Software Installation.............................................................. 3-24
3.3.8 Run Operating Software ........................................................................ 3-26
3.3.9 System Set up & Test ............................................................................ 3-27
Contents I
3.3.10 Training ................................................................................................ 3-28
4 Units and Modules................................................................................ 4-1
4.1 Sample/Reagent Probe Unit................................................................................. 4-1
4.1.1 Introduction .............................................................................................. 4-1
4.1.2 Components and Structure ...................................................................... 4-1
4.1.3 Installation................................................................................................ 4-4
4.2 Sample Disk Unit .................................................................................................. 4-4
4.2.1 Introduction .............................................................................................. 4-4
4.2.2 Components and Structure ...................................................................... 4-4
4.2.3 Servicing .................................................................................................. 4-5
4.3 Reagent Disk Unit............................................................................................... 4-11
4.3.1 Introduction ............................................................................................ 4-11
4.3.2 Components and Structure .................................................................... 4-12
4.3.3 Servicing the Reagent Disk Unit............................................................ 4-15
4.4 Reaction Disk Unit .............................................................................................. 4-21
4.4.1 Introduction ............................................................................................ 4-21
4.4.2 Components and Structure .................................................................... 4-22
4.4.3 Replacing Components and Parts ......................................................... 4-23
4.4.4 Installing Reaction Disk Drive Part ........................................................ 4-23
4.4.5 Installing Reaction Disk Motor Assembly............................................... 4-25
4.4.6 Installing Coder Sensor Assembly......................................................... 4-26
4.4.7 Installing Reaction Compartment Assembly .......................................... 4-27
4.4.8 Installing Reaction Disk Assembly......................................................... 4-28
4.5 Mixer Unit............................................................................................................ 4-29
4.5.1 Introduction ............................................................................................ 4-29
4.5.2 Components and Structure .................................................................... 4-29
4.5.3 Installation.............................................................................................. 4-31
4.6 Photometric Unit ................................................................................................. 4-32
4.6.1 Introduction ............................................................................................ 4-32
4.6.2 Components and Structure .................................................................... 4-33
4.6.3 Adjustment of Photometer ..................................................................... 4-35
4.6.4 Replacing Tungsten-halogen Lamp....................................................... 4-41
4.6.5 Replacing Optical Assembly .................................................................. 4-42
4.7 Wash Unit ........................................................................................................... 4-43
4.7.1 Introduction ............................................................................................ 4-43
4.7.2 Functions ............................................................................................... 4-43
4.7.3 Structure and Installation ....................................................................... 4-44
4.8 ISE Unit(optional)................................................................................................ 4-46
4.8.1 Introduction ............................................................................................ 4-46
4.8.2 Components and Structure .................................................................... 4-46
5 Hydropneumatic System ..................................................................... 5-1
5.1 Introduction ........................................................................................................... 5-1
5.2 Function Block Diagram ....................................................................................... 5-3
5.3 Schematic Diagram of Fluidic System.................................................................. 5-4
5.4 Connectors ........................................................................................................... 5-5
5.5 Tubing ................................................................................................................. 5-12
5.6 Solenoid Valves .................................................................................................. 5-32
5.7 Layout of Hydropneumatic Drawer ..................................................................... 5-34
5.8 Structure of Air Pump Assembly ......................................................................... 5-37
5.9 Water Supply Module(optional) .......................................................................... 5-39
5.10 Key Components............................................................................................. 5-42
II Contents
6 Hardware ............................................................................................... 6-1
6.1 Overview............................................................................................................... 6-1
6.2 Safety Precautions................................................................................................ 6-1
6.3 Circuit boards........................................................................................................ 6-1
6.4 Layout of the boards ............................................................................................. 6-4
6.5 Detaching and Assembling Circuit Boards ........................................................... 6-4
6.6 Function of board.................................................................................................. 6-4
6.6.1 Control Framework .................................................................................. 6-4
6.6.2 Main Board .............................................................................................. 6-5
6.6.3 Three-disk Drive Board............................................................................ 6-6
6.6.4 Three-probe Drive Board ......................................................................... 6-7
6.6.5 Pre-amp Board ........................................................................................ 6-8
6.6.6 AD Conversion Board .............................................................................. 6-8
6.6.7 Reagent Refrigeration Board................................................................... 6-9
6.6.8 Level Detection Board ............................................................................. 6-9
6.6.9 Clot Detection Board.............................................................................. 6-10
6.6.10 Pump/Valve Drive Board...................................................................... 6-10
6.6.11 Reaction Disk Temperature Control Board .......................................... 6-11
6.6.12 Preheat Temperature Control Board.................................................... 6-11
6.6.13 Communication Board ......................................................................... 6-11
6.7 Power Supply Module......................................................................................... 6-11
6.7.1 Features of Power Supply Module ........................................................ 6-12
6.7.2 Block Diagram ....................................................................................... 6-13
6.8 Connection Diagram ........................................................................................... 6-15
7 Replacement of Other Components and Parts .................................. 7-1
7.1 Overview............................................................................................................... 7-1
7.2 Enclosure and Panel............................................................................................. 7-1
7.3 Replacing Valves and Tanks................................................................................. 7-6
7.4 Installing Sample Syringe ................................................................................... 7-10
7.5 Installing Reagent Syringe.................................................................................. 7-11
7.6 Installing Power Supply Assembly...................................................................... 7-12
8 Service and Maintenance..................................................................... 8-1
8.1 Preparation ........................................................................................................... 8-1
8.1.1 Tools......................................................................................................... 8-2
8.1.2 Wash Solution.......................................................................................... 8-2
8.1.3 Others ...................................................................................................... 8-2
8.2 Daily Maintenance ................................................................................................ 8-2
8.2.1 Checking Sample/Reagent Syringes....................................................... 8-2
8.2.2 Checking/Cleaning Sample Probe........................................................... 8-3
8.2.3 Checking/Cleaning R1/R2 Probes........................................................... 8-3
8.2.4 Checking/Cleaning Sample/Reagent Mixers ........................................... 8-3
8.2.5 Checking Connection of Deionized Water............................................... 8-3
8.2.6 Checking Waste Tubing........................................................................... 8-4
8.2.7 Checking Vacuum/Pressure Pumps ........................................................ 8-4
8.2.8 Checking Printer/Printing Paper .............................................................. 8-5
8.2.9 ISE Unit (optional).................................................................................... 8-5
8.3 Weekly Maintenance ............................................................................................ 8-6
8.3.1 Cleaning Sample Probe........................................................................... 8-6
8.3.2 Cleaning R1/R2 Probes ........................................................................... 8-8
8.3.3 Cleaning Sample/Reagent Mixers ......................................................... 8-10
8.3.4 Cleaning Sample/Reagent Bar Code Reader Windows........................ 8-11
Contents III
8.3.5 Cleaning Sample Disk/Compartment .................................................... 8-11
8.3.6 Cleaning Reagent Disk/Compartment ................................................... 8-12
8.3.7 Cleaning Panels of Analyzing Unit......................................................... 8-13
8.3.8 Cleaning Reaction Cuvettes .................................................................. 8-14
8.3.9 Checking Photometer ............................................................................ 8-14
8.3.10 Checking Concentrated Wash Solution ............................................... 8-19
8.4 Two-week Maintenance ...................................................................................... 8-19
8.4.1 Maintaining Hydropneumatic Components............................................ 8-19
8.5 Monthly Maintenance.......................................................................................... 8-20
8.5.1 Cleaning Wash Well of Sample Probe................................................... 8-20
8.5.2 Cleaning Wash Well of R1/R2 Probes................................................... 8-21
8.5.3 Cleaning Wash Well of Sample/Reagent Mixers................................... 8-22
8.5.4 Cleaning Sample Probe Rotor............................................................... 8-23
8.5.5 Cleaning R1/R2 Probes Rotors ............................................................. 8-24
8.5.6 Cleaning Sample/Reagent Mixers Rotors ............................................. 8-25
8.5.7 Checking Wash Unit .............................................................................. 8-26
8.5.8 Checking Hydropneumatic Drawer........................................................ 8-29
8.5.9 Cleaning Air Filter, Oil Mist Separator, Mist Separator .......................... 8-30
8.5.10 Replacing Reaction Cuvette ................................................................ 8-30
8.6 Three-month Maintenance ................................................................................. 8-33
8.6.1 Cleaning Dust Screens .......................................................................... 8-33
8.7 Six-month Maintenance ...................................................................................... 8-33
8.7.1 Replacing Lamp..................................................................................... 8-33
8.7.2 Replacing or Cleaning Air Screen.......................................................... 8-36
8.7.3 Cleaning Tanks, Floater Switch and Siphon Tube ................................. 8-36
8.7.4 Maintaining the Air Pump....................................................................... 8-36
8.7.5 Replacing Waste Tubing........................................................................ 8-37
8.7.6 Replacing First and Second Phase Washing Tubing on Wash Unit...... 8-37
8.7.7 Replacing DI Water Filter and the Tubing.............................................. 8-39
8.7.8 Replacing On-line Filters and Check Valves ......................................... 8-39
8.8 As-Needed Maintenance .................................................................................... 8-45
8.8.1 Unclogging Sample Probe ..................................................................... 8-45
8.8.2 Unclogging R1/R2 Probes ..................................................................... 8-50
8.8.3 Replacing Sample Probe ....................................................................... 8-54
8.8.4 Cleaning Wash Well of Sample Probe................................................... 8-55
8.8.5 Replacing R1/R2 Probes ....................................................................... 8-56
8.8.6 Replacing Sample/Reagent Mixers ....................................................... 8-56
8.8.7 Replacing Syringe Plunger Assembly.................................................... 8-58
8.8.8 Removing Air Bubbles ........................................................................... 8-60
8.8.9 Replacing Reaction Cuvette .................................................................. 8-61
8.9 Maintaining ISE Module(Optional)...................................................................... 8-64
8.9.1 Replacing Reagent Pack ....................................................................... 8-64
8.9.2 Replacing Electrodes............................................................................. 8-64
8.9.3 Replacing Tubing ................................................................................... 8-65
8.9.4 ISE Unit Storage (optional) .................................................................... 8-65
9 Test and Maintenance Software .......................................................... 9-1
9.1 Basic Operations .................................................................................................. 9-1
9.1.1 Logging On .............................................................................................. 9-1
9.1.2 Overview.................................................................................................. 9-1
9.1.3 Operating Commands.............................................................................. 9-3
9.2 Macro Instructions .............................................................................................. 9-10
9.2.1 Function ................................................................................................. 9-10
IV Contents
9.2.2 Detailed Operations ............................................................................... 9-10
9.3 Performance ....................................................................................................... 9-13
9.3.1 Accuracy and Repeatability of Absorbance ........................................... 9-13
9.3.2 Stability of Absorbance .......................................................................... 9-14
9.3.3 Linearity of Absorbance ......................................................................... 9-15
9.3.4 Absorbance Accuracy and Precision of Diluted Sample ....................... 9-15
9.3.5 Residue of Cuvette ................................................................................ 9-16
9.3.6 Sampling Accuracy and Precision of Sample Probe ............................. 9-16
9.3.7 Carryover of Sample Probe ................................................................... 9-16
9.3.8 Backwater .............................................................................................. 9-17
9.3.9 Stability and Drift of Absorbance............................................................ 9-17
9.4 Parameter ........................................................................................................... 9-19
9.4.1 Detailed Operations ............................................................................... 9-19
10 Troubleshooting ................................................................................. 10-1
10.1 Classification of Error Messages .................................................................... 10-2
10.2 Corrective Measures....................................................................................... 10-4
10.2.1 Failures of Operation Unit.................................................................... 10-4
10.2.2 Failures of Analyzing Unit .................................................................. 10-13
11 Calculation Methods .......................................................................... 11-1
11.1 Reaction Types ............................................................................................... 11-1
11.1.1 Endpoint ............................................................................................... 11-1
11.1.2 Fixed-time ............................................................................................ 11-5
11.1.3 Kinetic .................................................................................................. 11-8
11.2 Prozone Check.............................................................................................. 11-14
11.2.1 Antigen Addition ................................................................................. 11-14
11.2.2 Reaction Rate Method ....................................................................... 11-16
11.3 Serum Index.................................................................................................. 11-17
11.3.1 What is Serum Index.......................................................................... 11-17
11.3.2 Calculation of Serum Index................................................................ 11-18
Contents V
1 System Description
1.1 Overview
The BS-400/BS-420 is a fully-automated and computer-controlled chemistry analyzer
designed for in vitro quantitative determination of clinical chemistries in serum,
plasma, urine and CSF (Cerebrospinal fluid) samples. The BS-400/BS-420
Chemistry Analyzer consists of the analyzing unit (analyzer) and operation unit.
Wash unit
Sample mixer Reagent mixer
Sample probe
R1 probe Reaction disk
R2 probe Sample disk
Reagent disk
Hydropneumatic
assembly
1.3 Functions
The general working procedure of the BS-400/BS-420 is as follows:
3. The reagent disk rotates to R1 aspirate position, and R1 probe aspirates reagent
from a bottle on the reagent disk.
4. When washed for 8 phases, the reaction cuvettes are carried to the reagent
dispense position, and the R1 probe rotates to the reaction disk and dispenses the
reagent to a cuvette.
6. The sample disk rotates to the sample aspirate position, and the sample probe
aspirates designated amount of sample from specified sample tube.
7. The reaction cuvette with R1 dispensed rotates to the sample dispense position,
and the sample probe dispenses the sample in the reaction cuvette.
8. With sample dispensed, the reaction cuvette rotates to sample mixing position for
stirring.
10. The reaction disk with sample dispensed rotates to the R2 dispensing position,
and the R2 probe dispenses the reagent to a reaction cuvette.
11. With R2 dispensed, the reaction cuvette is carried to the reagent mixing position
for stirring.
12. During each period, the reaction cuvette receives photometric measurement
(absorbance reading taking) when passing by the photometric unit.
14. The reaction cuvettes in which reaction is finished are washed when passing by
the wash unit.
2.1.1 General
System
System structure
Sample type
Throughput
400 tests/hour, or
Analytical method
Reaction time
Maximum of 10 minutes
Reaction temperature
37±0.1℃
Test scope
Predilution
Operation mode
System and tests are configured via the operating software. Profiles and calculation
tests are allowed.
Data processing
Capable of storing and outputting various data and tables/graphs, and calculating
among different tests
Dimensions
Weight
250 kg
Emergent samples
Network connection
Blood collecting tube: Φ12×68.5 mm, Φ12×99 mm, Φ12.7×75 mm, Φ12.7×100 mm,
Φ13 X 75 mm, Φ13 X 95 mm, Φ13 X 100 mm;
Plastic tube: Φ12×68.5 mm, Φ12×99 mm, Φ12.7×75 mm, Φ12.7×100 mm, Φ13 X 75
mm, Φ13 X 95 mm, Φ13 X 100 mm.
Sample disk
90 positions, which include the positions for calibrators, contros, STAT samples and
diluted samples
STAT sample
Emergent samples can be inserted during measurement at any time and then run
with high priority.
Sample volume
Sample probe
One probe, which is capable of detecting liquid level, clots and obstruction (in
horizontal and vertical directions), and of tracking liquid level
Inside and outside of the probe are washed with carryover less than 0.1%.
The reagent bar code is in conformity with the NCCLS standard and also compatible
with various application environments. The total length of reagent bar code is within
15-30 digits.
Name Description
Symbology Codabar, I 2 of 5 (interleaved 2 of 5), code128, code39,
UPC/EAN and Code93
Maximum bar 0.19mm
code density
Total length 15-30 digits
Bar code User-defined
format and
contents
Max. width of 55mm
bar code level
Min. height of 10mm
bar code label
Max. ±5 degree
inclination
angle
Print quality Class A (ANSI MH10.8M)
Wide and 2.5:1 to 3.0:1
narrow ratio
Reagent refrigeration
Reagent dispensing
Reagent types
Reagent volume
Reagent disk
Ordinary reagent disk, including inner and outer circles, 80 positions in total
Reagent bottle
80 reagent bottles can be held on the reagent disk. Each reagent position can hold
common 100ml or 70ml bottles or Mindray outer-circle 20ml/40ml and Mindray
inner-circle 40ml/62ml bottles.
Reagent probe
Two separate probes, which are capable of detecting liquid level and obstructions (in
horizontal and vertical directions), and tracking liquid level
Inside and outside of the probe are washed with carryover less than 0.1%.
5mm
90
Stirring method
150-360µl
Photometric system
Wavelength
12 wavelengths, which are 340nm, 380nm, 412nm, 450nm, 505nm, 546nm, 570nm,
605nm, 660nm, 700nm, 740nm and 800nm
Light source
Gratings type
Wavelength accuracy
±2nm
150µl
Photodiode array
Measurement range
Resolution of photometer
0.001OD
Linear (one-point, two-point and multi-point), Logit-Log 4p, Logit-Log 5p, Spline,
Exponential, Polynomial and Parabola
Display
15” LCD
Operating system
Communication interface
RS-232
Printer
Input device
Keyboard, network
Output device
Storage device
Water consumption
<20L/hour
Operating environment
2.2.2 Timing
→ Go to next period
c. Dispensing R1
k. Aspirating R1
→ Go to next period
e. Aspirating R2
i. Dispensing R2
N=0 Rinse1
N=2 Rinse2 Rinse3
N=4 Rinse4 Rinse5
N=6 Rinse6 CB Rinse7
N=8 Rinse8
N=10 R1 P1
N=12 P2 P3
N=14 P4 P5
N=16 P6 P7
N=18 P8 P9
N=40 P30
1 2 3 4 5 6 7 8 10 22 90# period
1 2 3 4 5 6 7 8 10 22 52 90# period
1 2 3 4 5 6 7 8 10 22 52 90# period
Go to next cycle
R3(R1 probe)
1'24" M3(Reagent mixer)
1 2 3 4 5 6 7 8 10 22 52 90# period
Go to next cycle
R3(R1 probe)
1'24" R4(R2 probe)
The bearing platform (or ground) should be level with gradient less than 1/200.
The bearing platform (or ground) should be able to bear 350Kg weight.
The installation site should be free of corrosive gas and flammable gas.
The installation site should not be disturbed by great noise or power supply.
The system should not be placed near brush-type motors and electrical contacts
that are frequently powered on and off.
Do not use such devices as mobile phones or radio transmitters near the system.
Electromagnetic waves generated by those devices may interfere with operation
of the system.
Maximum 3000
Operation Unit
700
Analyzing Unit
Minimum 500
F
r
Minimum 500
o
n
1180 t
The distance between the power socket and the system should be less than 2.5
meters.
Make sure the power socket is grounded correctly. Improper grounding may lead
to electric shock and/or equipment damage.
Water flow: Continuous flow no less than 30L/hour, with peak no greater than
1L/minute. An internal water container (above 40L) is required for the water unit
which has a flow of 20L/hour.
a. The tubing between the inlet filter and OUTLET of the water supply module
must not exceed 8 meters.
b. The tubing between the INLET port of water supply module and cover of the
deionized water tank must not exceed 10 meters.
d. The tubing from VENT of the water supply module to the sewer must not
exceed 10m.
The tubing from the outlet on BS-400/BS-420 to the inlet filter must not exceed 2
meters.
The tubing from the outlet on BS-400/BS-420 to the waste sewer must not
exceed 5 meters.
The waste sewer must be higher than the ground within 100mm.
Drainage module:
b. The tubing between the outlet on analyzer and inlet of drainage module
must not exceed 5 meters.
c. The tubing between the outlet of drainage module and the sewer must not
exceed 10 meters.
Water supply
91 92
with pressure
Inlet filter
95
93
Maximum
of 100mm
94
High-concentration
waste bucket
Sewer Sewer
Standard Configuration
Inlet filter
92
95
94
Maximum
Sewer Water supply
of 1200mm
96 97 91 93 98
Optional Components
High-concentration waste(2m)
Normal-pressure 1
93 low-conc.waste(5m)
94
High-pressure
Outlet
low-conc.waste(5m)
96 97 91
93 94 98
There are three types of outlet tubing for the water unit: 1/4” tubing, 1/3” tubing and
1/2” tubing. All of these three tubings are made from hard materials.
If the outlet tubing of the water unit has an external diameter of 1/4”, you should use
an M22 adapter to connect the outlet tubing to the transparent 6*4 PU tubing of the
analyzer. Perform the following steps to apply the adapter:
2. Thread the 6*4 PU tubing through the nut cap and make it protrude about 3-5mm
from the middle hole in the cap; then apply a small stopper on the PU tubing,
avoiding loose connection between the two tubings.
3. Thread the 1/4” outlet tubing through the middle hole of the other nut cap, making
the tubing protrude for about 3-5mm from the middle hole; and then insert the
tubing into the stopper connecting the PU tubing. Check if the outlet tubing is
connected tightly to the adpater; if not, apply a small stopper on the outlet tubing.
5. If the outlet tubing of the water unit has an external diameter of 1/3”, you should
use an M23 adapter to connect the outlet tubing to the transparent 6*4 PU tubing
of the analyzer.
2. Make one copy of original parameter configuration list as back up. The list should
be kept carefully by both the service engineer and the end user.
3.3.2 Unpacking
1. Detach the wooden case as follows.
a. Gross weight is 350 kg, totally 8 people are needed to lay the wooden case
containing the main unit on the ground. Fork truck is recommended, if possible.
b. Loosen the screws on the top cover and side cover of the wooden case.
Use adjustable wrench to loosen the screws on the base. Remove the steel part.
Use adjustable wrench to loosen the four retaining screws on the feet.
Figure 3-17Feet
Use a hex wrench or a screwdriver to rotate the feet to the highest point. Please
make sure that the diameter of the hex wrench or the screwdriver should be 5-6
mm to avoid distortion.
The padding par t is fixed to the right side cover. Loosen the 2 retaining screws to
remove the padding part.
Move the instrument down to the floor. Put the wooden plate and retaining screws
aside in a specified place.
Remove the retaining screws as shown in the following figure3 and remove the
cover.
Remove the 9 screws shown in the following figure and remove the drawer
package fixing plate.
Pull the drawer out and check whether the drawer assembly is running smoothly
and tubings are well connected.
Remove the four retaining screws of the air pump from the base plate of the frame.
Please insert the cross-head screwdriver into the bottom of the screw hole to
remove the screw. It is not necessary to elevate the analyzer to locate the screws.
Take the reagent and sample probe out from the accessory. Please make sure to
put the white gasket into the mental adapter of the probes.
Remove the probe enclosure: Use both of your hands to pull the bottom of the
enclousure to both sides and lift it up.
Move the probe arm to the highest point and remove the retaining bolt and spring.
Install the probes on to the arms. Please ensure that the probe can move upward
and downward after fixed onto the retaining bolt.
After installation is completed, ensure the stop plate is shielding the collision
sensor.
z Rotate the button on VR1 clockwise until the LED light D2 is on;
Install the mixing bar retaining screw on the installing hole of the mixing bar. Do
not fasten it.
Fasten the retaining screw of the mixing bar. Check whether the mixing bar is vertical. If
not, reinstall it.
Check whether the sample disk and reagent disk is well installed. Check whether the
retaining screw is fastened.
Fill concentrated wash solution: pull the drawer out and open the cap of the
concentrated wash solution A and B. Fill the bottole with 1L concentrated wash
solution. Fasten the cap.
Connect the analyzer, PC, printer, water machine (or water supply module),
drainage module cable. Do not Place the Power to On.
Power
2. Move the three probes to the top of their wash wells. Adjust clockwise the resistor VR1
on the liquid level detection board, making LED D2 light on; and then adjust
counterclockwise the resistor VR1 to make LED D2 light off. The current resistance just
meets the requirements. Switch off the analyzing unit switch.
7. Open vacuum-pressure pump, wait for 30 seconds then access “fluidic pressure curve”
tab to check whether pressure remain at normal range.
8. Keep SV15 opened, remove auto wash unit to a container (volume larger than 300ml)
9. Execute water reservoir tank affusion and probe outer wash by “system prime”. Check
whether water exists in wash well, and water supplied in water reservoir tank. (last for
about 2 minutes)
3 4
12. Execute “auto dilute” function to generate enough diluted detergent A and B (3 minutes).
2 1
13. Execute wash unit diffusion by opening SV0, SV07, SV20, SV21, when outlet water
become stable from wash unit, close this 4 valves.
1
1
2 3
14. Install wash unit back and make system reset, check movement of all mechanical parts.
Turn on the computer. Access folder “MSDE” in system software Installation program,
Double click icon as below to start installation.
b) Click ”Next” to the next step, make sure 10G space is available on the target
disk. Then click “Next”.
2. Request for calibration and samples after editing them, and then debug the results.
4.1.1 Introduction
The sample/reagent probe unit consists of the sample probe, R1 probe and R2 probe
assemblies, which are often called Three-probe Assembly.
The sample probe assembly includes a sample probe, which aspirates sample from
the sample tube and then dispenses it into reaction cuvette. The R1/R2 probe
assembly includes R1 probe and R2 probe, which respectively aspirate R1/R3 and
R2/R4 and then dispense them into reaction cuvettes.
Generally, all of the three probes are capable of detecting liquid level and
obstructions in the horizontal and vertical directions.
Additionally, the probes are also able to limit their mechanical motions or lock
themselves when power failure occurs.
The drive part supports the probe arm and drives the arm to move vertically or
horizontally, so that the sample probe and reagent probes move among different
positions.
The drive part includes the horizontal movement structure and vertical movement
structure. Both structures have stepper motors, synchronous belt wheel and
synchronous belt. Integrated with a bracket, the two structures finally drive vertically
or horizontally the probe arm via the spline shaft.
Besides the structural difference, the three probe assemblies differ from each other in
the upper motor plate and also the horizontal and vertical sensors. See Figure 4-2.
(a)
(c)
(a) Sample probe assembly (b) R1 probe assembly (c) R2 probe assembly
a. Holding sample tubes: Sample containers (tube, microtube, etc) with samples
are placed on the sample disk, and then the sampling structure aspirates
sample and dispenses them into reaction cuvette.
b. Timely feeding: The sample disk must carry specified sample tube to the
aspirate position for aspiration according to the predefined timing. The sample
disk is driven by the drive part.
c. Sample identification: The sample disk must be able to identify the samples
automatically. This function is achieved by the bar code reader system.
Sample disk: The sample disk holds the samples and in conjunction with the drive
part carries them to the aspirate position. Driven by the drive part, the sample disk
rotates around its axis.
Drive part: The drive part drives the sample disk to carry samples timely to the
aspirate position. During operation, the motor drives the axis to rotate via the
synchronous belt.
Bar code reader: The bar code reader identifies the samples correctly by scanning
the bar code label on sample tubes.
Sample compartment: The sample compartment is used to shield the light beam
sent from the bar code reader and separate the sample disk from other components.
4.2.3 Servicing
The location of the sample disk in the whole unit is shown in Figure 4-4.
See Figure 4-5.The sample disk assembly is compose of upper sample disk, lower
sample disk, tube rack, tube holder, handle and axis. Driven by the stepper motor and
synchronous belt, the sample disk rotates clockwise, carrying the sample tubes to the
aspirate position according to predefined timing. Then the sample probe aspirates
certain volume of sample and dispenses it into corresponding reaction cuvette.
a. Fix the handle to the silk-sided sample disk using two M4X12 cross pan head
screws. (Torque: 8-11kgf·cm)
b. Screw the four alignment pins to the upper sample disk. (Torque: 8-11kgf·cm)
c. Identify the two sides of the lower sample disk(The screw hole on reversed side
is protruded). Install the 90 tube racks to the obverse side of the lower disk, and
then secure the four alignment pins using four M3×8 socket head screws.
(Torque: 4.2-5.2kgf·cm)
d. Install the tube holders on the upper sample disk and ensure they are clicked in
place.
e. Set the sample disk components on the drive part from top to down, ensuring
the pins on the drive part are aligned correctly to the holes on upper sample
disk.
1. Replacing motor
b. Loosen the four M4×12 socket head screws that fix the motor on the mounting
plate.
c. Push the motor and remove the synchronous belt from the smaller belt wheel.
d. Remove the four M4×12 socket head screws that fix the motor, then remove the
motor and mounting plate.
e. Install the smaller belt wheel on a reliable motor and ensure the belt wheel is
installed at a height specified in Figure 4-7. (Add lock glue to the two M4×8 socket
head screws. Torque: 1.2N·m)
f. Fix the motor to the mounting plate using four M4×12 socket screws with plain
washer. (Torque: 2.0 N·m)
g. Install the motor mounting plate to the supporting rod using four M4×12 socket
screws with plain washer and spring washer. Please note not to tighten the screws
now.
a. Remove the synchronous belt from the motor. See steps a-c in 1. Replacing
motor.
b. Unplug the cables from the home position sensor and disk coder.
c. Use a wrench to remove the three M3×8 socket head screws, and then remove the
coder sensor bracket from the base plate of the analyzer.
d. Use a wrench to remove the four M5×20 socket head screws from the base plate,
and then remove the sample disk bearing upwards. Remove the components of the
drive assembly according to Figure 4-6.
a. Use three M5×20 socket head screws with plain washer to fix the sample disk
bearing to the base plate of the analyzer. (Torque: 4.0N·m). Please note not to touch
the sensor.
c. Use three M3×8 socket head screws to fix the sensor to the base plate and then
connect the sensor cable.
4. Replacing sensor
a. Unplug the home position sensor cable (BA40-21-61760) and disk coder sensor
cable (BA40-21-61761).
b. Use a wrench to remove the three M3×8 socket head screws that fix the coder
sensor bracket to the base plate, and then remove the coder sensor bracket.
d. Use three M3×8 socket head screws with plain/spring washers to fix the coder
sensor to the base plate of the analyzer.
a. Place two sponge cushions to two sides of the glass window. Please note not to
contaminate the glass window. Stick a sponge adjusting spacer to the dust shield and
ensure the spacer is aligned to the corresponding hole on the dust shield.
b. Place the prepared glass window in the groove of the sample compartment and
secure it with the dust shield, then tighten them with four M3×8 cross pan head
screws.
c. Use two M3×8 cross pan head screws to fix the conductive brush to the sample
compartment.
d. Use two M4×16 socket head screws with plain/spring washers to fix the sample
compartment to the upper-front beam(see Figure 4-4), and connect the conductive
brush to the upper-front beam. Be sure to align the sample compartment to the drive
e. Install the sample bar code assembly according to Figure 4-9. Use three M5×16
socket head screws with plain/sponge washers to fix the sample bar code assembly
to the base plate (See Figure 4-4). Connect the bar code reader cable and fix it to its
bracket.
f. Fix the sample disk components to the drive part, and place the bar code fixture on
position 53 of the sample disk (See Figure 4-10).
g. Check the connection of all cables on the analyzer and then switch on the power
supply. The system resets mechanically. Then the sample bar code reader emits light
beams. Be sure not to stare into the light beams. Otherwise your eyes may get hurt.
h. Adjust the sample bar code reader properly so that the light beam from it can pass
through the gap on the fixture. Use four M3×8 socket head screws to secure the bar
code reader to the two adjusted directions.
i. Install the front panel and front plate 1 successively, and then cap the sample disk.
a. Remove the sample disk cover, and then remove the front plate 1 and front panel.
c. Loosen the three M5×16 socket head screws and then remove the sample bar
code assembly.
a. Remove the sample disk cover, and then remove the front plate 1 and front panel.
b. Unplug the cable of the sample bar code reader and loosen the clip. Loosen the
two M5×16 socket head screws and then remove the sample bar code reader.
c. Use two M5×16 socket head screws with plain/sponge washers to secure a new
sample bar code reader to the bracket.
d. Adjust the sample bar code reader according to steps e, h, g and f in 1. Installing
Sample Bar Code Assembly(including sample compartment) .Please note not to
shield the light entrance of bar code reader with the sponge spacer.
e. Install the front panel and front plate 1 successively, and then cap the sample disk.
4.3.1 Introduction
1. The reagent disk unit is capable of refrigerating and keeping the reagents at 4-10
℃ in 24 hours a day, so that the reagents are always steady and will not volatile.
2. The reagent disk is driven by the drive module and rotates clockwise or
counterclockwise, carrying each reagent bottle to the aspirate position.
The reagent disk unit consists of reagent disk module, reagent refrigeration module,
and reagent disk drive module and bar code assembly. See Figure 4-11.
The reagent disk module is used to hold reagent bottles and rotates clockwise or
counterclockwise, carrying each reagent bottle to the aspirate position for reagent
aspirating. The reagent disk module includes the handle, reagent bottles, bottle
holder and reagent disk base.
The reagent refrigeration module is used to provide refrigeration function and keep
the reagents in a low-temperature environment, so that the reagents are always
steady and will not volatile. The reagent refrigeration module is composed of
refrigeration compartment, heated layer, refrigerating plate, insulation layer, radiator,
mounting plate, adjusting plate and the upper/lower air ducts.
The drive module consists of motors, belt wheel, synchronous belt, coder, sensor,
bearing assembly fixed axis, bearing assembly shaft and sleeve.
A reagent bar code reader is provided to input reagent information automatically. The
reagent bar code assembly is composed of the bar code reader and the anti-fog
device.
The BS-400/BS-420 employs semiconductor refrigeration, which is cool wind plus air
duct. The refrigeration compartment has inner diameter of Ø444mm and outer
diameter of 484mm. The refrigeration board is 10mm thick. From the bottom of the
compartment protrudes four ridges(height: 6mm), which are attached to the
refrigeration plates closely. The radiator is fixed to the bottom of the heated layer via
springs and mounting plates, and secures the refrigerating plate, which is pressed to
the refrigeration ridges and controlled by a spring. Below the heated layer embeds
screws to fix the mounting plates, isolating the refrigeration board from the outside.
There are two special air ducts located on two sides of the base plate to reject heat.
The Microscan MS-3 LASER reader (single-line, low density and lateral) is employed
on the BS-400/BS-420 in view of its bar code type, density, depth of field and
functions comparing with all bar code readers in China and foreign countries.
If the light beams of the bar code reader are emitted not in a specific angle to the
surface of bar code label, the emitted light is in the same direction with the reflected
light. If the reflected light is too strong, it may affect the scanning performance of the
bar code reader. Therefore, the reflected light must be weakened. The isolation glass
should be inclined in certain angle with the normal line, so that the reflected line (light
3 in following figure) cannot reach the bar code reader. The installation angle of the
isolation glass is adjustable from 0° to 30° and therefore be determined to be 5°.
Installation of modules:
The reagent disk module includes the handle, reagent bottles, bottle holder and
reagent disk base. See Figure 4-15.
Installation procedure:
1. Use two M4×12 cross pan head screws to fix the two handles to the reagent disk
bracket.
2. Use four M4×8 cross pan head screws to fix the reagent disk bracket to the
base.
Precautions:
See the figure below. The reagent disk base should be installed with the un-grooved
side upwards.
Installation procedure:
1. Fix the rubber cushion and condensation tubing connector with four M3×12 cross
pan head screws; apply water-proof glue in the circumference of the refrigeration
connector; remove the nut and spring washer from the temperature sensor connector
and use a transparent rubber washer instead; use the reagent compartment
thermistor fixture (BA40-J09) to guide through the sensor and tighten it, then apply
some water-proof glue in the circumference of the sensor.
3. Coat the word side of the refrigerating plate with heat glue(0.1-0.2mm thick) and
then place it in the groove of the insulation plate with glue side downwards, guide the
cable through the cable groove, then stick a heat film to the no-word side of the
refrigerating plate. Finally, apply some water-proof glue in the circumferences of the
two insulation plates.
4. Place the two radiators in the corresponding grooves of the reagent compartment
and place four washers and springs in sequence. Install the mounting plates and
secure them with four M4×16 stainless socket head screws with plain/spring washer,
then apply some water-proof glue in the circumferences of the two radiators.
5. Fix the radiator duct to the bottom of the refrigeration compartment using eight
M4×8 socket head screws with plain/spring washer.
6. Stick the waterproof cushion to the glass window in the refrigeration compartment.
7. Use four M3×10 cross countersunk head screws to fix the prepared anti-fog device
to the side of the refrigeration compartment.
8. Use four M3×6 cross pan head screws to fix the dust shield with sponges to the
mounting plate.
Precautions:
The refrigeration module drives the reagent disk and consists of motors, belt wheel,
synchronous belt, coder, sensor, bearing assembly fixed axis, bearing assembly shaft
and sleeve.
Installation procedure:
2. Hammer the A5×10 and A4×16 cylindrical pins to corresponding holes on the
bearing assembly, and integrate the bearing assembly shaft with the fixed axis,
then press 6004 bearing to the corresponding position on the shaft.
3. Sleeve stop washer to the threaded part of the fixed axis, then screw M20
retaining nut to the tension degree that the bearing can rotate freely and the stop
washer can clip to the gap on the retaining nut.
4. Insert an A4×16 cylindrical pin to the bigger belt wheel; use six M3×8 cross pan
head screws to connect the coder and belt wheel, and then tighten the coder to
the bearing shaft with six M4×16 socket head screws with plain/spring washer.
2. The end of cylindrical pin should be level to the lower end of the bearing shaft.
3. The bearing shaft should not be blocked or moved up and down abnormally.
A reagent bar code reader is provided to input reagent information automatically. The
reagent bar code assembly is composed of the bar code reader and the anti-fog
device.
Installation procedure:
1. Use two M3×8 socket head screws with plain/spring washer to fix the small
bracket of reader to the large support. Be sure to guide the screws through the
middle holes.
2. Install the reader to the small bracket and secure it with two M3×8 socket head
screws with plain/spring washer.
1. Be sure not to contaminate the glass window of the reader. Otherwise the
scanning performance may be degraded.
2. The screws to secure the reader should not be tightened unless you have
adjusted the reader properly.
1. Place a rubber cushion in the groove of the anti-fog device mounting plate.
3. Use six M2×8 cross pan head screws(torque: 1.5-2.5kgf.cm) to fix the heat
board to the mounting plate with its grooved side upwards.
5. Coat little heat glue to the heater on the downward side of the
overheat-protection switch, and then use a M3×12 socket head screw to press
out the overheat-protection switch.
6. Stick a sponge spacer to the dust shield of the bar code reader.
Precautions:
1. The window on the rubber cushion should be level to that on the mounting plate.
4. The window on the sponge spacer should be level to that on the dust shield, so
4.4.1 Introduction
The reaction disk assembly holds reaction cuvettes and rotates clockwise, carrying
the cuvettes to specified positions for sample/reagent dispensing and stirring, and
wash solution dispensing. Reagents and sample react with each other in reaction
cuvette. Also the reaction disk assembly provides a constant-temperature
environment for the reaction.
The figure below shows the positions on the reaction disk. No.4 is for stirring R2,
No.14 is for dispensing sample, No.15 is for dispensing diluted sample, No.44 is for
dispensing R2, No.51 is for dispensing R1, No.64 is for stirring sample, No.65 is for
stirring diluted sample, and No.83-90 is for washing cuvettes.
Washing position
(83-90#)
Reaction Disk
R1 dispensing
position (51#)
R2 mixing
position
R2 dispensing
position (44#)
Sample dispensing
position(14#)
Diluted sample dispensing
position(15#)
Drive part
Motor assembly
Reaction disk assembly: Used to hold reaction cuvettes, provide a
constant-temperature environment for reaction of reagent and sample in cuvettes,
Drive part: Used to drive the reaction disk assembly to rotate and carry reaction
cuvettes to specified positions for sample/reagent dispensing and stirring, and wash
solution dispensing. During operation, the motor drives the axis to rotate via the
synchronous belt.
Motor assembly: Used to provide force which drives the reaction disk assembly to
rotate via the belt and two belt wheels.
Coder sensor assembly: Used to find the mechanical zero position and count the
valid edges of the coder.
Reaction disk
assembly
Base plate
Framework
See Figure 4-22 for the structure of the reaction disk assembly.
2) Thread four M5×20 screws with spring washer through the bottom of the base
plate to fix the drive part on it. See Figure 4-26.
2. Install the motor on the mounting plate and install the small belt wheel on the motor
axis. See Figure 4-28.
3. Use four M4×12 socket head screws with plain/spring washer to fix the mounting
plate to the stand bars. Please note not to tighten the screws right now and not
reverse the connector of the motor. See Figure 4-29.
4. Install the belt on the big and small belt wheels, adjust the tension of the belt using
the fixture, and then tighten the four M4×12 socket head screws.
2) Use three M4×20 cross pan head screws to secure the reaction compartment to
the stand bars, and then tighten the three screws.
Precautions:
1) Before installing the reaction compartment, make sure you have installed the
reaction disk drive part, motor assembly and optical measurement assembly.
2) The three M4×20 cross pan head screws with plain washer should be used to fix
the reaction compartment to the stand bars.
2. Use three M5×16 socket head screws to fix the reaction disk assembly to the rotor
sleeve. See Figure 4-32.
3. Install the skylight cover to the drive plate with two retaining screws. See Figure
4-33.
4.5.1 Introduction
The mixer unit includes the sample mixer assembly and reagent mixer assembly.
Both mixer assemblies are equipped with a mixer, which is used to stir the liquid in
reaction cuvette.
Additionally, the mixers are also able to limit their mechanical motions or lock
themselves when power failure occurs.
Working positions of the two mixer assemblies: wash wellÆ reaction disk.
The mixer assembly consists of the drive part and the mixer arm. See Figure 4-34.
The drive part supports the mixer arm and drives the arm to move vertically or
horizontally, so that the mixers move among different positions.
The drive part includes the horizontal movement structure and vertical movement
structure. Both structures have stepper motors, synchronous belt wheel and
synchronous belt. Integrated with a bracket, the two structures finally drive vertically
or horizontally the mixer arm via the spline shaft.
The mixer arm consists of a mixer, motor, enclosure, etc, which are integrated by the
arm base.
The main difference between the two mixer assemblies is the upper plate of motor.
See Figure 4-35.
(a) (b)
4.5.3 Installation
Figure 4-36 Installing two mixer assemblies
4.6.1 Introduction
Chemistry analyzer is a typical precision instrument which features in optics,
mechanics, electronics and logarithm. The spectrophotometer is one of the key
components of the instrument and determines directly the precision and accuracy of
measurement by the system.
Open the shielding box of AD collection board (See Chapter 6), connect two probes
of the oscillograph to the AD start signal(RC and GND) and analog signal of channel
1(VG1-VG12), then connect the earth terminal to the ground. (Only the earth terminal
of one probe should be connected to the ground.)The channels, wavelength and test
points are listed in the following table.
Chan 1 2 3 4 5 6 7 8 9 10 11 12
nel
Wave 340 380 412 450 505 546 570 605 660 700 740 800
lengt
h
Test VG1 VG2 VG3 VG4 VG5 VG6 VG7 VG8 VG9 VG10 VG11 VG12
Point
The peak value of a normal signal is 5V. Both channel 1 and 2 should be adjusted to
2V, so that the photoelectric wave can be observed easily. Ensure the coupling mode
of the two channels is DC. Set up proper sampling interval to get waves easily. Set
the sampling mode to AUTO. See the figure below:
Sampling interval
100KS/s
2. Use the multimeter to test the power supply adapter and check if the voltage at J16
is within 12.1-12.2V(lamp brightness is approximately within 235-239). If not, select
the Parameter tab and then select Reaction Disk Unit in the Unit field, adjust the
lamp brightness until satisfied. (The greater the parameter, the higher the voltage)
After adjusting the parameters, turn off the lamp and then turn it on so that you can
get adjusted voltage.
4. First test the waves at 340nm, set up the Circles to 1 and (cuvette)Position to 1,
and then select the Rotate and Measure button.
5. When the oscillograph displays the complete waves circularly, press STOP on the
oscillograph. The waves are frozen and stop going.
6. Find the waves for the five cuvettes in which water is dispensed. (In order to find
the waves easily, water should be dispensed to cuvettes No.55-60). Generally, the
five waves of the cuvettes with water are higher and flatter than those of other
cuvettes.
If the waves are flat, the yellow waves refer to AD start signals (In a bundle of
collected signals, the rightmost means 340nm and the leftmost means 800nm), and
the red waves refer to the photoelectric analog signals.
Check if the AD start signal at 340nm is in the middle of the photoelectric analog
signal (See the figure below). If yes, the photoelectric collecting position is correct.
If the AD start signal at 340nm is in the decreasing part of the photoelectric analog
signal instead of its middle (See the figure below), the photoelectric collecting position
is not correct and must be adjusted by moving the coder sensor of the reaction disk
left or right.
Check if the waves of all channels are flat according to the step mentioned above. If
not, the pre-amplification board may go wrong and the optical assembly should be
replaced.
1. You are not recommended to extend the service of the lamp by adjusting the signal
gain.
2. The signal gain of the photoelectric unit has been configured properly before the
analyzer leaves the factory. When an alarm occurs indicating weak light, replace the
lamp instead of adjusting the signal gain. After replacing the lamp, check the new
lamp by executing Cuvette/Lamp Check on the Daily Maint. page of the operating
software.
3. If the gain for 340nm is 50 and the absorbance of water blank is less than or close
to the alarm limit, replace the new lamp as follows:
NOTE
Before testing the photoelectric gain, make sure that the lamp has been
on for at least 10 minutes; otherwise the lamp is not steady.
b. If the AD value exceeds the range, select the Parameter tab and then select
Main Unit in the Unit field, select Inquire to view the gain parameters of each
channel (When the gain parameter increases, the corresponding AD value will
decrease), adjust the water blank AD value of each channel within 47000-49000.
2. Use a cross-head screwdriver to loosen the two screws that fix the air duct in front
of the light source. Remove the air duct in front of the light source.
3. Use a cross-head screwdriver to loosen the two screws that fix the rear plate of
the lamp housing, and then remove the lamp housing by its upper plate, left plate,
right plate and rear plate.
4. Pinch the lamp base with one hand, and press the lever on the left of the base,
finally remove the lamp.
4. Remove the cover on the air duct of the light source assembly; use a hex
wrench to loosen the M3 retaining screw that fixes the fiber bundle on the front
plate of lamp housing; then unplug the fiber bundle.
5. Loosen the three screws that fix the heat chamber; raise the heat chamber with
one hand and lift up the optical assembly with the other hand so that the front
lens assembly is disconnected from the heat chamber.
Precautions:
2. The fiber bundle is bound loosely by a clip on the support of the heat chamber.
Hold the fiber bundle carefully so that it will not touch the axis of the reaction disk.
When removing the fiber bundle, remove it from the clip; then thread the fiber bundle
back into the clip during installation.
3. After removing the old optical assembly, place it in the installation package together
with the fiber bundle, and then bring the package back or send it to our company for
servicing.
4.7.1 Introduction
In order to use the reaction cuvettes repeatedly, the BS-400/BS-420 is integrated with
the wash unit, which is used to:
4.7.2 Functions
A reaction cuvette containing analyzed liquid passes successively the washing
positions 1-8. The wash unit includes 8 aspirate probes (Pd1-Pd8) and 8 dispense
probes(Pc1-Pc8), which are respectively located above positions 1-8.During washing
process, the corresponding solenoid valve is turned on before the wash probes lower
down, and then the dispense probes dispense wash solution in corresponding
cuvettes while lowering down. During the dispensing process, the dispense probes
also act as the overflow probes. Then the corresponding solenoid valve is turned on
and the waste liquid is discharged into the high-/low-concentration waste tanks.
The following figure shows the structure of the wash unit and the detailed procedure
for washing cuvettes.
1. When the cuvette containing analyzed liquid reaches position 1, the reaction
liquid is aspirated by Pd1, and then Pc1 dispenses wash solution D1 to the
cuvette.
2. In the next period, the cuvette is carried to position 2. Pd2 aspirates the wash
solution D1, and then Pc2 dispenses wash solution D2 to the cuvette.
3. In the third period, the cuvette is carried to position 3. Pd3 aspirates the wash
solution D2, and then Pc3 dispenses deionized water to the cuvette.
4. In the fourth period, Pd4 aspirates the deionized water, and then Pc4 dispenses
deionized water in the cuvette.
5. In the fifth and sixth periods, step 4 is repeated. When Pc6 dispenses wash
solution in the sixth period, the reaction disk rotates, carrying the cuvette to the
lamp site for photometric measurement. Whether the cuvette is washed clean is
determined by the measuring result(water blank).
6. In the seventh period, Pd7 aspirates the deionized water. This operation is
repeated in the eighth period. Pd7 and Pd8 are equipped respectively with a
wipe block on their ends. A very small space exists between the inside cuvette
and the wipe block, therefore, the water drop on the cuvette wall can be wiped
so that the cuvette is dried.
The following figures show the structure of ISE unit, ISE module, pump module and
reagent module.
5.1 Introduction
1. The fluidic system of BS-400/BS-420 includes the sampling system and washing
system.
2. The sampling system is equipped with three probes, two mixers and three syringes.
The sample syringe is 100µl and the two reagent syringes are 500µl.
3. The inside and outside of the three probes and the outside of the two mixers are
washed via pressure driving. The inside of the three probes are cleaned with
deionized water.
4. Wash well: Inner diameter of waste tubing is 0.5”. There are five wash wells and 7
normal waste liquids, which include reagent refrigeration waste, reaction disk
overflow waste, etc.
5. The reaction cuvettes are washed in 8 phases: 1) Phase 1-2: Cuvette is washed with
wash solution; 2) Phase 3-6: Cuvette is washed with deionized water; 3) Phase 7-8:
Cuvette is dried and wiped. During washing process, the wash solution and
deionized water are dispensed into cuvettes by pressure, and the waste liquids are
removed and drained by vacuum.
6. Water supply: An external water supply module or water unit is provided to supply
water. Therefore, requirements of water pressure and flow are imposed on the water
supply device.
7. Waste: Plastic hollow tubing is employed to collect the waste liquid. The waste
tubing should be installed at certain height so that the waste liquid can be drained by
gravity. Waste drainage: Low-concentration waste is drained to the sewer using the
waste pump; while high-concentration waste is drained to an external
high-concentration waste bucket that is equipped with a liquid level sensor.
9. Drive source: The reaction cuvettes are washed automatically by air pump driving.
11. The BS-400/BS-420 uses three types of compressed air: 25psig (for draining waste
liquid), 10psig (for supplying water and wash solution), and 5psig (for supplying
concentrated wash solution). The waste liquids are aspirated and drained by
vacuum within -50k--86kPa (15-25inHg).
12. Before washing cuvettes, the deionized water and diluted wash solution A/B are
preheated to 30-37℃.
13. A separate solenoid valve is provided to wash the outside and inside of three probes
and the outside of the two mixers.
14. Wash solution diluting is performed using a buffer. When the low-concentration
waste is used out, the liquid level sensor alarms. When the system receives the
alarm signals, it turns on successively the valve for driving concentrated wash
solution A, valve for driving concentrated wash solution B and valve for controlling
deionized water. Then the wash solution is diluted according to the dilution ratio that
has been configured.
15. The wash unit is able to divides the wash solution. During washing, the deionized
water is preheated and divided into 4 parts and then dispensed into reaction
cuvettes.
16. The pneumatic module is located on lower right of the analyzer in the form of drawer,
which can be pulled out for 500mm.
Gravity waste
Pressure flow
2.1 collecting tube
Vacuum/pneu
matic module
Pressure flow
Vacuum/ Pressure
Filter and Pressure Pressure waste
compression regulating/
silencer switch collecting tube
pump reducing valves
Solenoid Water
High-conc. Water
Vacuum tank Pressure tank Safety valve valves fro air Sewer supply
waste bucket unit
tubing device
External module
Cuvette
Wash solution Water supply
washing
module module
assembly
Connector.Tee,200Barb,1/16"ID,Nat
7 T407 M6Q-030069--- ural PP
Connector.Tee,200Barb,1/16"ID,Nat
8 T408 M6Q-030069--- ural PP
Tubing to tubing Y-connector,
9 T410 M6Q-030024--- 3140-06-00'legris'
Tubing to tubing Y-connector,
10 T411 M6Q-030024--- 3140-06-00'legris'
Connector. Straight
11 T412 M6Q-030061--- Through,Classic,1/4"&1/8"ID,PP
Wash unit
Connector. Straight
12 T413 M6Q-030061--- Through,Classic,1/4"&1/8"ID,PP
Right-angle connector
13 T415 M6Q-030020--- 3109-06-10'legris'
Right-angle connector
14 T416 M6Q-030020--- 3109-06-10'legris'
Right-angle connector
16 T418 M6Q-030020--- 3109-06-10'legris'
Right-angle connector
17 T419 M6Q-030020--- 3109-06-10'legris'
Connector. Straight
20 T422 M6Q-030061--- Through,Classic,1/4"&1/8"ID,PP
Connector. Straight
21 T423 M6Q-030061--- Through,Classic,1/4"&1/8"ID,PP
Tubing to tubing Y-connector,
T425 M6Q-030024--- 3140-06-00'legris'
Right-angle connector
42 T317 M6Q-030015--- 3175-06-10'legris'
Right-angle connector
43 T318 M6Q-030020--- 3109-06-10'legris'
Right-angle connector
44 T319 M6Q-030020--- 3109-06-10'legris'
Right-angle connector
45 T320 M6Q-030020--- 3109-06-10'legris'
Right-angle connector
46 T321 M6Q-030015--- 3175-06-10'legris'
Connector. Inside-threaded,
106 T001 M6Q-030035---
right-angle,3192-06-13
Connector, straight-through
109 T004 M6Q-030052---
3116-06-00
Connector. Inside-threaded,
115 T010 M6Q-030035---
right-angle,3192-06-13
Right-angle connector
116 T011 M6Q-030015---
3175-06-10'legris'
Right-angle connector
117 T012 M6Q-030020---
3109-06-10'legris'
Connector. Inside-threaded,
119 T014 M6Q-030035---
right-angle,3192-06-13
Right-angle connector
126 T021 M6Q-030015---
3175-06-10'legris'
Connector, straight-through
130 T025 M6Q-030052---
3116-06-00
Connector. Inside-threaded,
133 T028 M6Q-030035---
right-angle,3192-06-13
Right-angle connector
140 T035 M6Q-030020---
3109-06-10'legris'
Connector. Inside-threaded,
143 T038 M6Q-030035---
right-angle,3192-06-13
Right-angle connector
145 T040 M6Q-030020---
3109-06-10'legris'
Right-angle connector
146 T041 M6Q-030020---
3109-06-10'legris'
Connector. Inside-threaded,
148 T043 M6Q-030035---
right-angle,3192-06-13
Connector, straight-through
171 T601 M6Q-030052---
3116-06-00
Connector. Straight-through,
Panel
5.5 Tubing
Table 5-2 Fluidic tubing
Tubi
Mod Product Affiliated
No. ng Module Length
ule Number Assembly
No.
Tubing.1/8"X1/4
M90-00002 ",R-3603 Wash/preheat
1 C01 120
5--- AAC02007,Tyg assembly
on
Tubing.1/16"X1/
3001-10-07 8",S-50-HL Wash/preheat
2 C02 100
069 AAX02002,Tyg assembly
on
Tubing.1/8"X1/4
M90-00002 ",R-3603 Wash/preheat
Wash/preheat unit
3 C03 120
5--- AAC02007,Tyg assembly
on
Tubing.1/16"X1/
3001-10-07 8",S-50-HL Wash/preheat
4 C04 60
069 AAX02002,Tyg assembly
on
Tubing.1/8"X1/4
M90-00002 ",R-3603 Wash/preheat
5 C05 300
5--- AAC02007,Tyg assembly
on
Tubing.1/8"X1/4
M90-00002 ",R-3603 Wash/preheat
6 C06 450
5--- AAC02007,Tyg assembly
on
Tubing.1/8"X1/4
M90-00002 ",R-3603 Hydropneumat
61 D06 150
5--- AAC02007,Tyg ic drawer
on
Tubing.1/8"X1/4
M90-00002 ",R-3603 Hydropneumat
62 D07 150
5--- AAC02007,Tyg ic drawer
on
Tubing. Soft
precision PU
M6G-0200 Hydropneumat
63 D08 tubing 50
26--- ic drawer
(4MMX6mm).
Transparent
Tubing.1/8"X1/4
M90-00002 ",R-3603 Hydropneumat
64 D09 50
5--- AAC02007,Tyg ic drawer
on
Tubing.1/8"X1/4
M90-00002 ",R-3603 Hydropneumat
65 D10 50
5--- AAC02007,Tyg ic drawer
on
Tubing.1/8"X1/4
M90-00002 ",R-3603 Hydropneumat
66 D11 50
5--- AAC02007,Tyg ic drawer
on
precision PU
module
M6G-0200 Hydropneumat
78 J01 tubing 45
26--- ic drawer
(4MMX6mm).
Transparent
Tubing. Soft
precision PU
M6G-0200 tubing Hydropneumat
84 J07 950 ic drawer
26--- (4MMX6mm).
Transparent
Tubing. Soft
precision PU
M6G-0200 tubing Hydropneumat
85 J08 550 ic drawer
26--- (4MMX6mm).
Transparent
Tubing.1/8"X1/4
M90-00002 ",R-3603 Hydropneumat
86 J09 AAC02007,Tyg 80 ic drawer
5---
on
FEP Tubing,
Diba
0040-10-32 2-4066-C100-0 Hydropneumat
87 J10 02, 150 ic drawer
301
0.040''ID×0.066'
'OD
Tubing.1/8"X1/4
M90-00002 ",R-3603 Hydropneumat
88 J11 AAC02007,Tyg 80 ic drawer
5---
on
Tubing. Soft
precision PU
M6G-0200 tubing
90 J13 80
26--- (4MMX6mm).
Transparent
FEP Tubing,
Diba
0040-10-32 2-4066-C100-0 R1 probe
91 J14 02, 110 assembly
301
0.040''ID×0.066'
'OD
FEP Tubing,
Diba
0040-10-32 2-4066-C100-0 R2 probe
92 J15 02, 240 assembly
301
0.040''ID×0.066'
'OD
FEP Tubing,
Diba
0040-10-32 2-4066-C100-0 R2 probe
93 J16 02, 1380 assembly
301
0.040''ID×0.066'
'OD
FEP Tubing,
Diba
0040-10-32 2-4066-C100-0 Sample probe
94 J17 02, 1050 assembly
301
0.040''ID×0.066'
'OD
FEP Tubing,
Diba
0040-10-32 2-4066-C100-0 Sample probe
95 J18 02, 1050 assembly
301
0.040''ID×0.066'
'OD
Tubing. Soft
precision PU
M6G-0200 tubing Wash well
97 J20 500 assembly
26--- (4MMX6mm).
Transparent
Tubing. Soft
precision PU
M6G-0200 tubing Wash well
98 J21 500 assembly
26--- (4MMX6mm).
Transparent
Tubing. Soft
precision PU
M6G-0200 tubing Wash well
99 J22 500 assembly
26--- (4MMX6mm).
Transparent
Tubing. Soft
precision PU
M6G-0200 tubing Wash well
100 J23 500 assembly
26--- (4MMX6mm).
Transparent
Tubing. Soft
precision PU
M6G-0200 tubing Wash well
101 J24 330 assembly
26--- (4MMX6mm).
Transparent
Tubing. Soft
precision PU
LQ0 M6G-0200 tubing Hydropneumat
102 1900
Water supply module
Tubing. Soft
precision PU
LQ0 M6G-0200 tubing Hydropneumat
103 75 ic drawer
2 26--- (4MMX6mm).
Transparent
Tubing. Soft
precision PU
LQ0 M6G-0200 tubing Hydropneumat
105 70 ic drawer
4 26--- (4MMX6mm).
Transparent
Tubing. Soft
precision PU
LQ0 M6G-0200 tubing Hydropneumat
106 100 ic drawer
5 26--- (4MMX6mm).
Transparent
Tubing. Soft
precision PU
LQ0 M6G-0200 tubing Hydropneumat
107 120 ic drawer
6 26--- (4MMX6mm).
Transparent
Tubing. Soft
precision PU
LQ0 M6G-0200 tubing Hydropneumat
108 120 ic drawer
7 26--- (4MMX6mm).
Transparent
Tubing. Soft
precision PU
LQ0 M6G-0200 tubing Hydropneumat
109 120 ic drawer
8 26--- (4MMX6mm).
Transparent
Tubing. Soft
precision PU
LQ0 M6G-0200 tubing Hydropneumat
110 120 ic drawer
9 26--- (4MMX6mm).
Transparent
Tubing. Soft
precision PU
LQ1 M6G-0200 tubing Hydropneumat
111 220 ic drawer
0 26--- (4MMX6mm).
Transparent
Tubing. Soft
precision PU
LQ1 M6G-0200 tubing Hydropneumat
113 120 ic drawer
2 26--- (4MMX6mm).
Transparent
Tubing. Soft
precision PU
LQ1 M6G-0200 tubing Hydropneumat
114 140 ic drawer
3 26--- (4MMX6mm).
Transparent
Tubing. Soft
precision PU
LQ1 M6G-0200 tubing Hydropneumat
115 150 ic drawer
4 26--- (4MMX6mm).
Transparent
Tubing. Soft
precision PU
LQ1 M6G-0200 tubing Hydropneumat
116 160 ic drawer
5 26--- (4MMX6mm).
Transparent
Tubing. Soft
precision PU
M6G-0200 tubing Air pump
117 G01 470 assembly
26--- (4MMX6mm).
Transparent
Hydropneumatic module
Tubing. Soft
precision PU
M6G-0200 tubing Air pump
118 G02 40 assembly
25--- (4mmX6mm),
yellow
Tubing. Soft
precision PU
M6G-0200 tubing Hydropneumat
119 G04 435 ic drawer
25--- (4mmX6mm),
yellow
Tubing. Soft
precision PU
M6G-0200 tubing Hydropneumat
121 G06 250 ic drawer
25--- (4mmX6mm),
yellow
Tubing. Soft
precision PU
M6G-0200 tubing Hydropneumat
122 G07 260 ic drawer
25--- (4mmX6mm),
yellow
Tubing. Soft
precision PU
M6G-0200 tubing Air pump
123 G08 205 assembly
25--- (4mmX6mm),
yellow
Tubing. Soft
precision PU
M6G-0200 tubing Air pump
124 G09 170 assembly
25--- (4mmX6mm),
yellow
Tubing. Soft
precision PU
M6G-0200 tubing Air pump
125 G10 60 assembly
25--- (4mmX6mm),
yellow
Tubing. Soft
precision PU
M6G-0200 tubing Air pump
126 G11 65 assembly
25--- (4mmX6mm),
yellow
Tubing. Soft
precision PU
M6G-0200 tubing Air pump
127 G12 90 assembly
26--- (4MMX6mm).
Transparent
Tubing. Soft
precision PU
M6G-0200 tubing Air pump
129 G14 70 assembly
23--- (4mmX6mm),
red
Tubing. Soft
precision PU
M6G-0200 tubing Air pump
130 G15 50 assembly
23--- (4mmX6mm),
red
Tubing. Soft
precision PU
M6G-0200 tubing Air pump
131 G16 140 assembly
23--- (4mmX6mm),
red
Tubing. Soft
precision PU
M6G-0200 tubing Air pump
132 G17 50 assembly
23--- (4mmX6mm),
red
Tubing. Soft
precision PU
M6G-0200 tubing Air pump
133 G18 70 assembly
23--- (4mmX6mm),
red
Tubing. Soft
precision PU
M6G-0200 tubing Air pump
134 G19 360 assembly
23--- (4mmX6mm),
red
Tubing. Soft
precision PU
M6G-0200 tubing Hydropneumat
135 G20 110 ic drawer
23--- (4mmX6mm),
red
Tubing. Soft
precision PU
M6G-0200 tubing Hydropneumat
137 G22 1125 ic drawer
23--- (4mmX6mm),
red
Tubing. Soft
precision PU
M6G-0200 tubing Hydropneumat
138 G23 75 ic drawer
23--- (4mmX6mm),
red
Tubing. Soft
precision PU
M6G-0200 tubing Hydropneumat
139 G24 500 ic drawer
23--- (4mmX6mm),
red
Tubing. Soft
precision PU
M6G-0200 tubing Hydropneumat
140 G25 300 ic drawer
23--- (4mmX6mm),
red
Tubing. Soft
precision PU
M6G-0200 tubing Hydropneumat
141 G26 75 ic drawer
23--- (4mmX6mm),
red
Tubing. Soft
precision PU
M6G-0200 tubing Hydropneumat
142 G27 490 ic drawer
23--- (4mmX6mm),
red
Tubing. Soft
precision PU
M6G-0200 tubing Hydropneumat
143 G28 270 ic drawer
23--- (4mmX6mm),
red
Tubing. Soft
precision PU
M6G-0200 tubing Hydropneumat
145 G30 280 ic drawer
24--- (4mmX6mm),
blue
Tubing. Soft
precision PU
M6G-0200 tubing Hydropneumat
146 G31 65 ic drawer
24--- (4mmX6mm),
blue
Tubing. Soft
precision PU
M6G-0200 tubing Hydropneumat
147 G32 125 ic drawer
24--- (4mmX6mm),
blue
Tubing. Soft
precision PU
M6G-0200 tubing Hydropneumat
148 G33 85 ic drawer
24--- (4mmX6mm),
blue
Tubing. Soft
precision PU
M6G-0200 tubing Hydropneumat
149 G34 195 ic drawer
22--- (4mmX6mm),
green
Tubing. Soft
precision PU
M6G-0200 tubing Hydropneumat
150 G35 190 ic drawer
22--- (4mmX6mm),
green
Tubing. Soft
precision PU
M6G-0200 tubing Hydropneumat
151 G36 365 ic drawer
22--- (4mmX6mm),
green
Tubing. Soft
precision PU
M6G-0200 tubing Hydropneumat
153 G38 400 ic drawer
22--- (4mmX6mm),
green
Tubing. Soft
precision PU
M6G-0200 tubing Hydropneumat
154 G39 100 ic drawer
22--- (4mmX6mm),
green
Tubing. Soft
precision PU
M6G-0200 tubing Hydropneumat
155 G40 100 ic drawer
22--- (4mmX6mm),
green
Tubing. Soft
precision PU
M6G-0200 tubing Hydropneumat
156 G41 200 ic drawer
24--- (4mmX6mm),
blue
Tubing. Soft
precision PU
M6G-0200 tubing Hydropneumat
157 G42 55 ic drawer
24--- (4mmX6mm),
blue
Tubing. Soft
precision PU
M6G-0200 tubing Hydropneumat
158 G43 60 ic drawer
24--- (4mmX6mm),
blue
Tubing. Soft
precision PU
M6G-0200 tubing
159 G45 65
25--- (4mmX6mm),
yellow
Tubing. Soft
precision PU
M6G-0200 tubing
161 G47 70
23--- (4mmX6mm),
red
Tubing. Soft
precision PU
M6G-0200 tubing
162 G48 140
23--- (4mmX6mm),
red
Tubing. Soft
precision PU
M6G-0200 tubing
163 G49 370
24--- (4mmX6mm),
blue
Tubing. Soft
precision PU
M6G-0200 tubing
164 G51 80
22--- (4mmX6mm),
green
Tubing. Soft
precision PU
M6G-0200 tubing
165 G52 380
22--- (4mmX6mm),
green
Tubing. PU
tubing
M6G-0200 4mmX2.5mm, Drainage
166 W01 150 assembly
02--- transparent,
Drainage module
PU0425
Tubing. Soft
precision PU
M6G-0200 tubing Drainage
167 W02 245 assembly
26--- (4MMX6mm).
Transparent
Tubing. PVC
M6G-0200 knitted tubing, Drainage
169 W04 12mmX18mm, 360 assembly
28---
subtransparent
Tubing. PVC
M6G-0200 knitted tubing, Drainage
170 W05 12mmX18mm, 360 assembly
28---
subtransparent
Tubing. PVC
M6G-0200 knitted tubing, Drainage
171 W06 12mmX18mm, 650 assembly
28---
subtransparent
Tubing. PVC
M6G-0200 knitted tubing, Drainage
172 W07 12mmX18mm, 410 assembly
28---
subtransparent
Tubing. PVC
M6G-0200 knitted tubing, Drainage
173 W08 12mmX18mm, 200 assembly
28---
subtransparent
Tubing. PVC
M6G-0200 knitted tubing, Drainage
174 W09 12mmX18mm, 350 assembly
28---
subtransparent
Tubing. PVC
M6G-0200 knitted tubing, Drainage
175 W10 12mmX18mm, 200 assembly
28---
subtransparent
Tubing. PVC
M6G-0200 knitted tubing, Drainage
176 W11 12mmX18mm, 160 assembly
28---
subtransparent
Tubing. Soft
precision PU
M6G-0200 tubing Drainage
178 W13 1300 assembly
26--- (4MMX6mm).
Transparent
Tubing. Soft
precision PU
M6G-0200 tubing Drainage
179 W14 1400 assembly
26--- (4MMX6mm).
Transparent
Tubing. PVC
M6G-0200 knitted tubing, Drainage
180 W15 12mmX18mm, 50 assembly
28---
subtransparent
Tubing. PVC
M6G-0200 knitted tubing, Drainage
181 W16 12mmX18mm, 80 assembly
28---
subtransparent
Tubing. PVC
M6G-0200 knitted tubing, Drainage
182 W17 12mmX18mm, 260 assembly
28---
subtransparent
Tubing. PVC
M6G-0200 knitted tubing, Drainage
183 W18 12mmX18mm, 460 assembly
28---
subtransparent
Tubing. Soft
precision PU
M6G-0200 tubing Drainage
184 W19 1930 assembly
22--- (4mmX6mm),
green
Tubing. Soft
precision PU
M6G-0200 tubing
185 W20 1350
26--- (4MMX6mm).
Transparent
25psig 10psig
Vacuum 5psig
Low-conc.
waste
Sensor
Fluid line Out In Air line Air out In Pressure tank out Air in
Outlet
Outlet
High-conc. Vacuum
waste tank
Sensor
Fluid line out Sensor In Air line Air out In out Air in
Outlet
Back
Left Right
Water tank
Front
Diluent B L. H.
sensor sensor
H. Water
Fluid line out sensor in Air line Pressure in In outlet out Water in
Outlet L.
sensor
Sensor
H.
Fluid line out sensor
in Air line
5psi IN
Refilling
position
h
Outlet was
ted k A
Sensor
Check
valve
As shown in the figure above, the water supply module consists of five components:
1. power supply; 2. diaphragm pump; 3. pressure switch; 4. inlet valve(inside
analyzer); 5. water tank inside of analyzer.
The internal water tank pressurizes water and supplies it to the wash unit of the
analyzer. Moreover, the water tank possesses liquid level detection device to detect
the water in it. When the water in the water tank is run out, the detection device
sends a signal to turn on the inlet valve 4, and water is supplied. When the water
tank is refilled full, the detection device sends a signal to turn off the inlet valve, and
water supplying is stopped.
The pressure switch 3 is controlled by the pressure that it detects, which can be
configured as needed. Suppose the ON pressure is P1 and OFF pressure is
P2(P1<P2).When the pressure of the tubing that is connected to the pressure
switch is greater than or equal to P2, the pressure switch is OFF; otherwise the
pressure switch is ON. If the pressure is within P1 and P2, the pressure switch
keeps unchanged. Suppose P1<P<P2, when the pressure is equal to P2, the
pressure switch is OFF. If the pressure decreases from P2 to P, the pressure switch
keeps OFF until to P1. Similarly, if the pressure increases from P1 to P, the pressure
switch keeps ON until to P2.
The power supply 1, diaphragm pump 2 and pressure switch 3 are connected in
series and constitute a closed circuit. The diaphragm pump 2 is powered by power
supply 1 and controlled by the pressure switch 3.When the pressure switch 3 is ON,
the circuit is connected and the diaphragm pump 2 is started; otherwise, the circuit
is disconnected and the diaphragm pump 2 is stopped.
See the figure above. The external water tank contains purified deionized water,
which is passed via inlet tubing to J1 connector of the water supply module and then
to the analyzer via J11 after being pressurized.
The drainage module pressurizes and discharges the waste liquid from the
analyzer.
The liquid floater sensor inside the waste buffer, upon the amount of waste in the
buffer, sends control signals to the PCB, which then turns on or off the waste pump
accordingly.
93 94 98
Drainage module
Function: Prevent pressure from going up. Overflow will be stopped in case of great
pressure.
Function: Adjust pressure to specified range. Switch between 5psi and 10psi.
Function: Turn on/off all fluidic or air tubing connected to the valve according to
received electric signals.
4. Check valve
Function: Enable the flow unidirectionally and prevent the liquid or air in the tubing
from flowing back.
NOTE
Do not confuse the check valve with a filter.
5. Pressure switch
6.1 Overview
This chapter describes the function of circuit boards in the BS-400/BS-420.
If you need to detach the circuit boards, you must first cut off the power of the
analyzer. Please wear gloves to protect the circuit boards from ESD (electrostatic
discharge) or release the charge first before detaching the circuit boards.
6 Hardware 6-1
BA40-30-61377 and with the computer. The first type includes
the serial port, network interface, USB port; and
(BA40-20-61376)
the latter type includes the standard DB9 serial
port, RJ45 network interface and the standard
USB port.
Note: Only the serial ports are now occupied on
the BS-400/BS-420, and others are reserved.
Reaction disk This board collects the temperature sensor #10
temperature control signals from the reaction disk and converts them
board into digital signals. This board provides an
SPI(Serial Port Interface) and a power supply
BA40-30-61375
interface for connecting to the three-disk drive
(BA40-20-61374) board via a slip ring.
Note: The same PCB is applied for collecting
reaction disk temperature and preheat
temperature. However, the two temperature
collecting boards differ from each other in the
BOM.
Wash solution This board processes, collects and converts into
preheat digital signals the temperature sensor signals of
temperature control the three wash solution flows. This board
board provides an SPI(Serial Port Interface) and a
power supply interface for connecting to the
BA40-30-73102
three-disk drive board.
(BA40-20-61374)
Note: The same PCB is applied for collecting
reaction disk temperature and preheat
temperature. However, the two temperature
collecting boards differ from each other in the
BOM.
Pump and valve This unit receives signal from the three-disk #6
drive board drive board and the three-probes drive board
and then makes the pumps and valves act;
BA40-30-61373
There are 5 level detection senses connecting
(BA40-20-61372) the three-disk drive board through this board.
Reagent This unit is used to cool the reagents, indicate #12
refrigeration board the temperature and drive the fan and defog
circuit.
BA40-30-61371
(BA40-20-61370)
Level detection This unit can test the reagent’s or the sample’s #9
board level and detects obstructions occurring to the
sample probe and reagent probes.
BA40-30-61369
(BA40-20-61368)
Clot detection By observing the pressure change inside the #5
board sample probe, this board detects the clots in the
sample probe.
BA40-30-61367
(BA40-20-61366)
AD conversion This unit can modify the analog signals from the #7
board pre-amp board and convert the analog signals
into digital signals. Also this board provides an
BA40-30-61365
SPI(Serial Port Interface) for connecting to the
(BA40-20-6136) main board.
6-2 6 Hardware
Preamplification This unit can converse the light signals into #8
board electrical analog signals by the photoelectric
diode.
BA40-30-61363
(BA40-20-61362)
Three-probe drive This unit can control and drive the first reagent #4
board probe, the second reagent probe, the sample
mixer and the reagent mixer to act.
BA40-30-61361
(BA40-20-61360)
Pressure detection This board detects the pressure of the air tubing
board that is connected to the vacuum compression
pump, and provides a serial port for
BA40-30-61447
communicating and transferring pressure data.
(BA40-20-61446)
AC pump This board acts as an adapter to connect the AC
connection board pump and its fan to the power supply. The AC
pump is powered by alternating current and
BA40-30-61781
connected to the power supply assembly; and
(BA40-20-61780) the fan is powered by direct current and
connected to the reagent refrigeration board.
Drainage module This board detects the signals indicating that the
control board low-concentration waste tank is full, and then
turns on the waste pump to discharge the waste,
BA40-30-61498
thus avoiding waste overflow. Also this board
(BA40-20-61497) sends the waste full signals to the computer,
which then gives alarms to remind the user to
empty the low-concentration waste time
immediately.
Inlet pump power This board turns on/off the inlet pump power
supply board according to the pressure signals of the inlet
tubing, so as to ensure safe pressure of the inlet
BA40-30-72955
tubing.
(BA40-20-72954)
Three-disk drive This unit can control and drive the three disks, #1
board wash unit, temperature control unit and other
related moving parts.
BA40-30-61359
(BA40-20-61358)
Power board This unit provides the power for the whole #3
machine.
12V board:
BA40-30-61623
(BA40-20-61622)
24V board:
BA40-30-61625
(BA40-20-61624)
Connection board:
BA40-30-61627
(BA40-20-61626)
6 Hardware 6-3
6.4 Layout of the boards
Figure 6-1 shows the circuit boards on the analyzer.
The analyzing unit(main unit) consists of the following units: the temperature control
system, the reaction system(include ISE), the photoelectric test system, the sample
and reagent delivery system, the mixing system and the auto clean unit etc.
communicating with the PC through the RS232 to send commands , reply data
and test results.
controlling the data acquired process of optical system .
controlling the moving units’ action and collecting the status signal.
6-4 6 Hardware
controlling the temperature adjustment system and collecting temperature status
signal.
6 Hardware 6-5
Figure 6-3 Function of the main control board
BDM
Power supply PC
debug
BDM UART
RTC
I2C
DEVICE
USB
EEPROM
SPI
CPU
FEC
SDRAMC PHY
SDRAM
USB
PCI
USB Device
Host
Flex Bus
FLASH
AD conversion board
All CPU on this board receive the orders from the main board through the RS232,
and decode it to act.
The CPU on board output signals to control the moving parts related with the
three disks.
This unit can receive the signals from the moving parts’ senses, signal protected
from collision of the auto clean unit and other status signals.
This unit can control the heater of the reaction disk (solid heater directly), the
pre-warm wash water and it can test the temperature of this two parts and the
environment temperature too.
This unit can receive signals from the liquid level senses and response according
the signals.
Figure 6-4 shows the function framework of the three-disk drive board.
6-6 6 Hardware
Figure 6-4 Three-disk-drive board
This unit can receive the orders from the main board through the RS232 and
transmit the test data to the main board.
This unit can control and drive the moving parts of the R1 probe, R2 probe, the
sample probe, the sample mixer, the reagent mixer, the valves, the syringes, etc.
This unit can receive the position signals from the moving parts’ senses. and the
signal for protecting probes from collision in horizontal orientation
This unit can test the liquid level according the signal from the level detection
board and receive the signals for protecting the probes from collision too .
This unit can test the clot signal of the sample probe through the interface with
the clot detection board and read the pressure value too.
Figure 6-5 shows the function framework of the three-probe drive board.
6 Hardware 6-7
Figure 6-5 Three-probe drive board
AD conversion board
12 photoelectric Gain Multi- Photoelectric
Pre-amp. signals AD data
regulating channel Main board
board Power collection Control
circuit selection signal
6-8 6 Hardware
6.6.7 Reagent Refrigeration Board
This board is independent compared with other circuit boards; the unit can control the
cooler chip on or off and then make the reagents cool; it can adjust the temperature in
the reagent carousel ; this unit can also drive the fan of the whole system and reflect
the fan’s signal to the three disks control-driver board; the detail is:
Figure 6-7 shows the function framework of the reagent refrigeration board.
The three level detection boards have the same construction and interface and
detect the reagent level and sample level individually with the high reliability,
especially the sample level detection.
The circuit boards generate the level detection signal when the probes touch the
liquid level; the depth that the probes dip into the liquid is not more than 2mm.
This unit can protect the probe from collision vertical ; it generates signal which is
sent to the three probes control-driver board.
Figure 6-8 shows the function framework of the level detection board.
6 Hardware 6-9
6.6.9 Clot Detection Board
The clot detection board of the BS-400/BS-420 main function:
This circuit board can draw a conclusion of ‘possible’ clot by judgment the change of
the pressure value when the sample probe aspirates the sample and send this signal
to the three probes control-driver board.
This circuit board can draw a conclusion of ‘convinced’ clot by judgment the change
of the pressure value when the sample probe aspirates the sample and send this
signal to the three probes control-driver board.
This circuit board can draw a conclusion of ‘air’ aspiration by judgment the change of
the pressure value when the sample probe aspirates the sample and send this signal
to the three probes control-driver board.
This circuit board can judge the clot cleared accord the change of the pressure value
when the probe is washed inside and outside in the wash pot and send the indicated
signal to the three control-driver board.
Figure 6-9 shows the function framework of the clot detection board.
Figure 6-10 shows the function framework of the pump and valve driver board.
6-10 6 Hardware
6.6.11 Reaction Disk Temperature Control Board
The reaction disk temperature control board collects signals from the reaction disk
temperature sensor and then converts them into digital signals. This board provides
an SPI interface and a heater power interface, and is connected to the three-disk
driver board via a slip ring.
NOTE
The same PCB is applied for the reaction disk temperature control
board and the preheat temperature control board. However, the two
temperature control boards differ from each other in the BOM.
providing the interfaces connecting the main control board: serial port, Ethernet
port, USB port
providing the port connecting the PC outside: DB9 serial port, RJ45 WEB port,
standard USB port
This circuit board is the relay station between the main control board and the PC
outside and it can protect and isolate the communication signal too.
The 24V board transforms the AC power to the A24V, B24V and A12V(the lamp
source).
The 12V board transforms the AC power to the other 12V(B12V and C12V) and 5V as
the system needs.
The power connection board has the function of relaying the AC power, controlling
the vacuum pump power, transforming to -12V, controlling the C12V and output of the
other voltages.
The power supply module provides all power through the interfaces on the power
connection board, and the 24V board, the 12V board and the connection board use
the plug board to board to connect.
The whole power system is a integrity module. It can be shielded and isolated from
the heat efficiency.
6 Hardware 6-11
The power supply module can be cooled by the fans.
6.7.1.1 Input
Input AC voltage: 90-264VAC
Frequency: 50/60±3Hz
Input power: 2KVA
Max instantaneous current: <40A
6-12 6 Hardware
6.7.1.7 DC Output: C12V – for Pump /Valve Board
Output current: 7A
Output voltage: 11.4-12.6V
Output ripple noise: <=120mVp-p
6 Hardware 6-13
Figure 6-11 Framework of power supply module
6-14 6 Hardware
6.8 Connection Diagram
Figure 6-12 Connection diagram 1
1 2 3 4 5 6 7 8
PC
D D
C o m m u n ica tio n
b o a rd
M a in c o n tro l b o a r d
C C
A D co n v er sio n
IS E T h ree-d isk d riv e b o a rd T h ree-p r o b e d riv e b o a r d
b o a rd
Pow er
m o d u le
R e ag en t, S am p le C lo t d etectio n L iq u id lev e l
P re-a m p S lip rin g
co d e scan m o d u le b o a rd d etectio n b o a rd
B B
APPROVALS DATE
V a cu u m p u m p
S lip rin g
co n n ectio n b o a rd DESIGN MINDRAY
CHECK
A TITLE A
M a in co n n e ctin g d ia g ra m
CHECK
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F i l e: Bytes:
h e re in a re p r o p e r ty o f S h en z h en M in d r ay B io -m e d ic a l E le ctr on ics C o.,L td . N o u se , c op ie s o r r ep r o d u c tion s sh ou ld b e m a d e o f CHECK
th is d r a w in g o r a n y p ar t (s) th e re o f fo r w h a te ve r p u rp o se n o r sh a ll a n y in fo r m a tion , d a ta , ca lcu latio n s, o r o th er c o n te n ts DWG NO. A -B A 4 0 -3 0 - REV 1 .0
D a t e: Time: c on ta in ed in th is d r aw in g b e d is sem in a te d w ith o u t p r io r w ritten p er m issio n o f S h en zh e n M in d ra y B io -m e d ica l E le ctr on ic s R&D
C o .,L td .
Software & Rev: Microsoft office Visio 2003 C H I E F E N G. SHEET 1 OF 16 S I Z E A3
1 2 3 4 5 6 7
6 Hardware 6-15
Figure 6-13 Connection diagram 2
1 2 3 4 5 6 7 8
BA40-21-61705
BA 40-20-61588
Three-disk drive
A24VOUT board BA40-20-61587
1 1 BA40-21-61749
BA 40-30-61359
ISE J3
2 GND 1 +12V
2 J1 1 shield Yellow J1 J1
2 GND
2 SIGN Black 1 2 3 +12V 1 2
1 2 1 2 4 GND
3 GND Blue
4 SIGN 3 4 5 +12V 3 4
3 4 Black 3 4 6 GND
5 G ND W hite 5 6 5 6
5 J4 7 +12V
6 6 SIGN Brown 5 6 7 8 8 GND 7 8 Pump/ valve drive board
1 A12VOUT 7 8 7 SIGN Red 9 +24V BA 40-30-61373
1 7 8 9 10 9 10
Lam p 8 SIGN Yellow 10 GND
J16 11 12 11 +24V
2 GND 11 12
2 12 +24V
B B
BA40-20-61910 BA40-20-61807
A
MINDRAY
Power m odule connecting
A
TITLE: diagram
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DWG NO. A-BA40-30- REV 1.0
Date: Time:
6-16 6 Hardware
Figure 6-14 Connection diagram 3
1 2 3 4 5 6 7 8
D Pre-am p board D
BA40-30-61363
2 4 6 8 2 4 6 8
J2 J3
1 3 5 7 1 3 5 7 J1 1 2 3
BA40-20-61486
SIG6 Orange
Orange
Yellow
Yellow
Green
AGND White
BA40-20-61484
BA40-20-61485
SGND Black
Green
White
Gray
2 AGND Black
Black
Blue
Gray
Blue
Blue
Red
Red
Red
AGND
SGND
1 VDD
3 VSS
SIG1
SIG2
SIG3
SIG4
SIG5
SIG1
SIG2
SIG3
SIG4
SIG5
SIG6
C C
1
2
3
4
5
6
7
2
3
4
5
6
7
8
8
2 4 6 8 2 4 6 8 J1 1 2 3
J2 J3
1 3 5 7 1 3 5 7
AD conversion board
BA40-30-61365
14 15 16 17 18 19 20 21 22 23 24 25
P1
B
1 2 3 4 5 6 7 8 9 10 11 12 13 B
10 DCP_CLK
18 AD_BUSY
11 DCP_DIN
19 AD_CLK
9 DCP_EN
6 AD_DIN
20 AD_RC
22 CH_A2
23 CH_A1
24 CH_A0
21 CH_A3
4 15GND
17 15GND
25 GND
13 GND
1 +15V
14 +15V
5 GND
16 VCC
8 GND
3 VCC
12 NC
15 -15V
2 -15V
7 NC
BA30-20-06552
J13 14 15 16 17 18 19 20 21 22 23 24 25
1 2 3 4 5 6 7 8 9 10 11 12 13
A
M ain board BA40-30-61356 MINDRAY A
Pre-am p board、 AD conversion
TITLE:
board connecting diagram
File: Bytes:
DWG NO. A-BA40-30- REV 1.0
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6 Hardware 6-17
Figure 6-15 Connection diagram 4
1 2 3 4 5 6 7 8
4
6
5
J12
1
3
2
FPGA_CONF_OE
BA40-20-61642
RSTCTL_REAC
BA40-20-61641
RSTCTL_AW
RSTCTL_RP2
RSTCTL_ST
RSTCTL_RT
RSTCTL_RP1
BA40-20-61588
SPI_CLOCK
RSTCTL_RM
Yellow
RSTCTL_TC
RSTCTL_SM
White
RXD_REAC
Black
TXD_REAC
RSTCTL_SP
4 12VGND Blue
5 12VGND Blue
SPI_DATA
Red
NCONFIG
RXD_AW
NCONFIG
TXD_AW
TXD_RP2
RXD_RT
RXD_RP1
RXD_RP2
TXD_RT
REV2_O
TXD_ST
RXD_RM
TXD_RP1
RXD_SM
RXD_ST
RXD_TC
TXD_RM
TXD_TC
TXD_SM
REV1_O
REV2_O
REV3_O
REV4_O
REV5_I
RXD_SP
TXD_SP
SPI_CS
REV5_I
REV6_I
DCLK
ASDO
DATA
DCLK
ASDO
DATA
GND
GND
GND
GND
NCE
NCS
GND
GND
GND
GND
GND
GND
GND
NCE
NCS
GND
GND
GND
6 GND
1 +12V
2 -12V
3 +5V
33
31
29
34
21
13
23
11
12
20
24
28
32
10
14
26
27
22
25
30
15
5
16
7
6
33
19
4
8
34
29
31
11
18
1
17
3
10
21
23
25
27
12
20
24
28
32
13
22
26
30
17
14
19
5
16
18
15
2
1
6
7
4
4
6
5
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34
J1
J10 1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 J6 1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33
1
3
2
C C
Ports: J1 ~ J15
M ain board FPG A JTAG : J12
BA40-30-61356
FPG A AS: J13
CPU BDM : J11
J15
Ports spare: J2、 J5、 J7、 J9、 J14
J4 J3
13 12 11 10 9 8 7 6 5 4 3 2 1 J8
2 4 6 8 10 2 4 6 8 10
25 24 23 22 21 20 19 18 17 16 15 14 1 3 5 7 9 1 3 5 7 9 1 2 3 4 5 6
BA 40-20-61589
BA40-20-61590
B B
BA 30-20-06552 BA40-20-61591
红色
7 PC_RESET
10 DCP_CLK
18 AD_BUSY
6 PWFBOUT
11 DCP_DIN
2 PC_RXD
19 AD_CLK
3 PC_TXD
ISE CO M
9 DCP_EN
6 AD_DIN
20 AD_RC
22 CH_A2
23 CH_A1
24 CH_A0
21 CH_A3
1
4 12GND
17 12GND
红色
5 GND
25 GND
13 GND
1 +12V
14 +12V
NC
5 GND
5 GND
8 GND
16 VCC
12 NC
3 VCC
15 -12V
3 RX+
2 -12V
10 NC
7 NC
2 TX-
1 TX+
1 NC
4 NC
6 NC
8 NC
9 NC
4 RX-
2
ISE_TXD
3
ISE_RXD
4
NC J3 J6
14 15 16 17 18 19 20 21 22 23 24 25 5
J61 GND 2 4 6 8 10
6 1 2 3 4 5 6
1 2 3 4 5 6 7 8 9 10 11 12 13 NC 1 3 5 7 9
7
ISE_CTS Communication board
8
AD conversion board ISE_RTS
9 BA40-30-61377
BA40-30-61365 NC
10
MINDRAY
NC
A A
6-18 6 Hardware
Figure 6-16 Connection diagram 5
1 2 3 4 5 6 7 8
D D
M ain board
BA40-30-61356
J3
J8
2 4 6 8 10
1 3 5 7 9 1 2 3 4 5 6
7 PC_RESET
Red
6 PWFBOUT
2 PC_RXD
3 PC_TXD
BA 40-20-61590
BA40-20-61589
5 GND
C C
5 GND
3 RX+
10 NC
2 TX-
1 TX+
1 NC
4 NC
6 NC
8 NC
9 NC
4 RX-
J3 J6
2 4 6 8 10
1 2 3 4 5 6
1 3 5 7 9
J2 J5
1 2 3 4 5 2 4 6 8
6 7 8 9 1 3 5 7
B B
7 PC_RESET
2 PC_RXD
3 PC_TXD
BA33-20-35270
5 GND
3 RX+
6 RX-
2 TX-
1 TX+
1 NC
4 NC
6 NC
8 NC
9 NC
7 NC
8 NC
4 NC
5 NC
RS232 W eb port
PC
A
MINDRAY
Com munication board connecting
A
TITLE: diagram
File: Bytes:
DWG NO. A-BA40-30- REV 1.0
Date: Time:
6 Hardware 6-19
Figure 6-17 Connection diagram 6
1 2 3 4 5 6 7 8
2
1 2 3 4 1 2 3 4 1 2 3 4
black
black
red
red
1 LEVEL green 3
1 LEVEL green 3
BA40-21-61727
BA40-21-61728
BA40-20-61906
BA40-20-61907
3
2 RAM_V_PHO 2
2 RAM_V_PHO 2
2 RAM_V_PHO 2
black 1
black 1
black 1
red 4
red 4
BA40-21-61776
red 4
D
1 LEVEL green
D
blue
blue
blue
2 GND
2 +12V
2 +12V
2 GND
1 +6V
2 +12V
1 +6V
1 GND
1 GND
1 GND
J29 1 2 J30 1 2 J21 1 2 J20 1 2 J22 1 2 J19 1 2 J23 1 2 J18 1 2
BA 40-21-61706
6brown B- 4brow n
1
6
B+ 3orange
First reagent arm 4 4orange
2
up/down motor
J4
3 3yellow A- 2yellow
BA40-10-
3
1 1red A+ 1red
4
BA40-21-61778
BA 40-21-61707
1
6 6brown B- 4brow n 1 VPP Red
J24 J3
First reagent arm 4 4orange B+ 3orange 2 VEE
2
rotation motor
J5
1 2 3 CLOG_YES 1 2
3 3yellow A- 2yellow
3
BA40-10-
4 CLOG_MAY
C 1 1red A+ 1red Ports: J1 ~ J32 3 4 3 4 C
5 N O_SAM
4
5 6 5 6
FPG A AS: J1 6 INT_CON
BA 40-21-61710
7 8 7 8
1
6 6brown B- 4brown 7 INT_EN
FPG A JTAG : J2 9 10 8 GND 9 10
4 4orange B+ 3orange
2
First reagent syringe
J6
m otor 9 RES_CLG _I
Three- probes drive board
BA40-30-61367
Clot detection
3
3 3yellow A- 2yellow
BA40-10- Port spare: J25 10 RES_CLG _O
BA40-30-61361
board
1 1red A+ 1red
BA 40-21-61711 4
1
6 6brown B- 4brown 1 VPP Red
J17 J2
2
3orange 2 VEE
Second reagent arm 4 4orange B+
J7
up/down motor 1 2 3 RXD_CLG 1 2
3
BA 40-21-61713 7 GND
6 6brown B- 4brown 8 GND
2
4
3
1 1red A+ 1red
J9
4 3 2 1
J11 J12 J13 J14 J15 J16
J10 4 3 2 1 4 3 2 1 4 3 2 1 4 3 2 1 4 3 2 1 4 3 2 1 4 3 2 1
3orange
3orange
3orange
3orange
3orange
3orange
3orange
3orange
4brown
4brown
4brown
4brown
4brown
4brown
4brown
4brown
2yellow
2yellow
2yellow
2yellow
2yellow
2yellow
2yellow
2yellow
1red
1red
1red
1red
1red
1red
1red
1red
BA40-21-61718
BA40-21-61721
BA40-21-61724
BA40-21-61720
BA40-21-61723
BA40-21-61716
BA40-21-61715
BA40-21-61726
A+
A+
B+
B+
3 3yellow A-
3 3yellow A-
6 6brown B-
6brown B-
A+
A+
A+
A+
A+
A+
B+
B+
4 4orange B+
B+
B+
B+
3 3yellow A-
3 3yellow A-
3 3yellow A-
3 3yellow A-
3 3yellow A-
3 3yellow A-
6brown B-
6brown B-
6 6brown B-
6 6brown B-
6brown B-
6 6brown B-
4 4orange
4 4orange
4 4orange
4 4orange
4 4orange
4 4orange
4 4orange
1 1red
1 1red
1 1red
1 1red
1 1red
1 1red
1 1red
1 1red
MINDRAY
6
6
6
6
A A
Three- probe drive board
Sample syringe m otor Reagent m ixer arm TITLE:
Second reagent Sample probe arm Sam ple probe arm
BA40-10-
Reagent mixer arm Sam ple m ixer arm Sample mixer arm connecting diagram (1)
File: Bytes: syringe m otor up/down m otor rotation m otor up/down m otor rotation motor up/down m otor rotation motor
BA40-10- BA40-10- BA 40-10- BA40-10- BA40-10- BA40-10- BA40-10-
DWG NO. A-BA40-30- REV 1.0
Date: Time:
6-20 6 Hardware
Figure 6-18 Connection diagram 7
1 2 3 4 5 6 7 8
D D
BA40-20-61641
RSTCTL_RP2
RSTCTL_RP1
RSTCTL_RM
RSTCTL_SM
RSTCTL_SP
NCONFIG
TXD_RP2
RXD_RP1
RXD_RP2
RXD_RM
TXD_RP1
RXD_SM
TXD_RM
TXD_SM
REV1_O
REV2_O
REV3_O
REV4_O
RXD_SP
TXD_SP
REV5_I
REV6_I
DCLK
ASDO
DATA
GND
GND
GND
GND
NCE
NCS
GND
GND
GND
33
34
31
29
12
20
24
28
32
21
23
25
27
11
10
22
26
13
30
14
17
16
18
2
19
4
8
1
15
6
3
7
BA40-21-61740
BA40-21-61729
1red VCC 1 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 1 VCC 1red
Sam ple probe arm up/down + 4white SU_PHO 2 J27 2 RS2_PHO 4w hite +
C 2black 1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 Second reagent syringe sense
sense
- GND 3 3 GND 2black C- BA40-21-
BA 40-21-
E 3green GND 4 4 GND 3green E
1red VCC 5 2 1 5 VCC
+ 4white + Second reagent probe arm
Sam ple probe rotation sense
C 2black
SR_PHO 6 4 3 6 R2_RAM _H_PHO
C protected from collision sense
BA40-21- - GND 7 1 2 7 GND - (H )
E 3green GND 8 6 5 8 GND E BA40-21-
3 4
C 1red
+ 4white VCC 9 8 7 9 VCC
+ C
Sam ple syringe sense C 2black
SS_PHO 10 5 6 10 RU1_PHO
C First reagent probe arm
- GND 11 10 9 11 GND - up/down sense
BA 40-21-
E 3green GND 12 7 8 12 GND E BA40-21-
12 11
1red VCC 13 9 10 13 VCC
Sam ple probe protected from + 4white 14 14 13 J28 +
C 2black
S_RAM_H_PHO 14 RR1_PHO
C
First reagent probe arm
collision sense (H)
- GND 15 16 15 11 12 15 GND rotation sense
3green -
BA40-21- GND 16 16 GND BA 40-21-
18 17 13 14
E 1red E
+ 4white VCC 17 15 16 17 VCC
+
Second reagent probe arm C 2black RU2_PHO 18 20 19 18 RS1_PHO
up/dow n sense - GND 19 Three- probe drive 17 18 19 GND
C
-
First reagent syringe sense
BA40-21- E 3green GND 20 22 21 20 GND E
BA 40-21-
24 23
board 19 20
1red 21
Second reagent probe arm + 4white VCC
RR2_PH O 22 26 25 BA40-30-61361 21 22
21
22
VCC
R1_RAM _H_PHO
+ First reagent probe arm
up/dow n sense C 2black GND 23 23 C protected from collision sense
- J26 23 24
GND - (H )
BA40-21-
E 3green GND 24 24 GND E BA 40-21-
NC 25 25 26
25 VCC
+ Reagent m ixer arm up/down
NC 26 27 28 26 RM U_PHO
C
27 sense
GND - BA 40-21-
29 30 28 GND E
1 6 31 32 29 VCC
30 RM R_PHO +
31 C Reagent m ixer arm rotation
2 7 33 34 GND - sense
B 32 GND E BA 40-21- B
35 36
+24V Yellow
+24V Yellow
GND Black
GND Black
10 GND Black
Red
-12V White
GND Black
GND Black
+12V Blue
3 8 J3
37 38 33 VCC
+
4 9 34 SM U_PHO
C sam ple m ixer arm up/down
BA40-21-61705 39 40 35 GND - sense
1 +5V
5 10 36 GND E BA40-21-
37
2
3
4
9
5
6
7
8
J32 VCC
J31 38 SMR_PHO
+ Sam ple m ixer arm rotation
39 GND
C sense
1 2 3 4 5 1 3 5 7 11 13 15 17 19
9 -
1 2 3 4 5 6 40 GND E BA40-21-
J14
6 7 8 9 10 2 4 6 8 10 12 14 16 18 20
R1_VALUE_OUT Green
R2_VALUE_OUTGreen
Green
S_VALUE_OUT Green
RES_VALUE5 Green
RES_VALUE6 Green
RES_VALUE7 Green
RES_VALUE8 Green
RES_VALUE4 Green
RES_VALUE1 Green
RES_VALUE2 Green
RES_VALUE3 Green
Black
Black
Black
Black
Black
Black
Red
Power m odule
Red
6 +12V
4 +12V
3 GND
5 GND
2 +12V
BA 40-30-61627
1 GND
BA40-21-61679?
BA40-21-61747
RM_VALUE
SM_VALUE
GND
GND
+5V
GND
GND
GND
+5V
MINDRAY
1 black
1 black
1 black
2 red
red
red
10
11
20
12
13
14
15
16
17
18
19
9
8
3
5
6
1
2
2
Inside valve
Inside valve
BA40-21-
BA40-21-
BA40-21-
2 4 6 8 10 12 14 16 18 20
valve
File: Bytes:
Pum p/valve drive board DWG NO. A-BA40-30- REV 1.0
Date: Time: BA40-30-61373
6 Hardware 6-21
Figure 6-19 Connection diagram 8
1 2 3 4 5 6 7 8
D D
BA40-21-61778
1 VPP Red
J24 2 VEE J3
BA40-20-
3 CLOG _YES 1
1 2 1 2 1 SIG+
4 CLOG _M AY 2 2 I+
3 4 3 4
5 NO_SA M 3 3 I-
C 5 6 5 6 J1 Pressure sensors C
6 INT_CON 4 SIG-
7 8 7 8 4 BA 40-20-
7 INT_EN 5 RA-
9 10 8 GND 9 10 5
6 RA+
9 RES_CLG _I 6
Three- probe drive 10 RES_CLG_O
board
Clot detection
BA 40-30-61361 board
1 VPP Red
BA40-30-61367
J17 2 VEE J2
1 2 3 RXD_CLG 1 2
4 TXD_CLG 3 4
3 4
5 PSEN _CLG
5 6 5 6
6 RST_CLG
7 8 7 GND 7 8
8 GND
BA40-21-61779
B B
A
MINDRAY A
6-22 6 Hardware
Figure 6-20 Connection diagram 9
1 2 3 4 5 6 7 8
D D
black
black
black
red
red
red
BA40-21-61775
BA40-21-61774 BA 40-21-61775
2 SIGN
2 SIGN
2 SIGN
1 GND
1 GND
1 GND
J1 1 2 J1 1 2 J1 1 2
Sam ple liquid level Ports: J1 ~ J3 First reagent level Second reagent liquid
detection board detection board level detection board
C BA40-30-61369 BA40-30-61369 C
Port spare for debug: J3 BA40-30-61369
J2 1 2 3 4 J2 1 2 3 4 J2 1 2 3 4
black 1
2 RAM_V_PHO blue 2
black 1
black 1
blue 2
2 RAM_V_PHO blue 2
1 LEVEL green 3
1 LEVEL green 3
red 4
red 4
red 4
1 LEVEL green
2 RAM_V_PHO
2 +12V
2 +12V
2 +12V
1 GND
1 GND
1 GND
J21 1 2 J20 1 2 J22 1 2 J19 1 2 J23 1 2 J18 1 2
Three-probe drive
B
board B
BA40-30-61361
A
MINDRAY A
Level detection board connecting
TITLE:
diagram
File: Bytes:
DWG NO. A-BA40-30- REV 1.0
Date: Time:
6 Hardware 6-23
Figure 6-21 Connection diagram 10
1 2 3 4 5 6 7 8
Sample disk bar code Reagent disk bar code Sam ple disk status LED
reader reader BA40-20-
BA40-20- BA40-20-
Red
Black
D
BA40-21-61771
D
Red
Red
BA40-21-61772
BA40-21-61773
10 shield
10 shield
5 GND
8 TRIG
8 TRIG
5 GND
2 RXD
2 RXD
1 LAMP
3 TXD
3 TXD
7 RTS
7 RTS
1 VCC
1 VCC
2 GND
6 CTS
6 CTS
9 NC
9 NC
4 NC
4 NC
BA 40-21-61751 J11 J12 J17
6brown B- 4brown 2 4 6 8 10 2 4 6 8 10
1
6
1 2
Reaction disk rotation 4 4orangeB+ 3orange 1 3 5 7 9 1 3 5 7 9
2
m otor
J3
3 3yellow A- 2yellow
BA40-10-
3
BA40-20-61642
1 1red A+ 1red
4
BA 40-21-61752 1 RXD_TC
6green B- 6green J24 2 TXD_TC
1
6 3 RSTCTL_TC J6
5blue +24V 5blue 4 GND
2
5
1 2 5 RXD_AW 1 2
Reaction disk rotation 4brown B+ 4brown 6 TXD _AW
3
4 3 4 3 4
m otor 7 RSTCTL_AW
J7
C 3orange A- 3orange C
8 GND
4
BA40-10- 3 5 6 5 6
2yellow +24V 2yellow Ports: J1 ~ J27, J31 9 RX D_ST
7 8 7 8
5
2 10 TXD_ST
1red A+ 1red FPG A AS: J18 9 10 11 RSTCTL_ST 9 10
6
1 12 GND
BA40-30-61356
11 12 13 RXD_RT 11 12
FPG A JTAG : J19
Three-disk drive
Main board
BA 40-21-61753 14 TXD _RT
13 14 13 14
6brown B-
1
4brown 15 RSTCTL_RT
6
board Ports spare: J13 ~ J16, J21, J25 15 16 16 GND 15 16
4 4orange B+ 3orange
2
Reagent disk rotation 17 18 17 RXD_REAC
BA40-30-61359 17 18
J4
m otor 18 TXD_REAC
3 3yellow A- 2yellow 3 19 20 19 RSTCTL_REAC 19 20
BA40-10-
20 GND
1 1red A+ 1red 21 22 21 22
4
25 26
6 6brown B- 4brown 24 GND
27 28 25 SPI_CS 27 28
2
29 30 27 SPI_DATA 29 30
m otor
3
3 3yellow A- 2yellow
BA40-10- 31 32 28 GND 31 32
29 DCLK
4
1 1red A+ 1red 33 34 33 34
30 NCONFIG
31 ASDO
B BA 40-21-61755 B
32 DATA
33 NCS
6 6brown B- 4brown
1
J8 34 NCE
J1
W ash unit up/down 4 4orangeB+ 3orange J22
2
m otor
J6
1 2 3
1 1red A+ 1red 7 8 9 10 11 12 5 6 7 8
4
Yellow
BA40-20-61909
Black
Red
2 SIGN
1 GND
3 VCC
8 SIGN Yellow
-12V Brown
+24V Yellow
BA40-21-61748
1 +24V Yellow
1 shield Yellow
6 SIGN Brown
4 SIGN Black
5 GND White
BA40-21-61749
Blue
3 GND Blue
7 SIGN Red
GND Black
GND Black
10 GND Black
2 SIGN Black
GND Black
11 GND Black
12 GND Black
+24V Yellow
+12V White
+5V
1 2 3
MINDRAY
4
6
2
3
9
7
8
5
J19 A
A
1 2 3 4 5 6 1 2 3 4 Reagent
Three-disk drive board
J15 J1 TITLE: connecting diagram 1
7 8 9 10 11 12 5 6 7 8 refrigeration board
File: Bytes: BA40-30-61371
Power m odule DWG NO. A-BA40-30- REV 1.0
Date: Time: BA40-30-61627
6-24 6 Hardware
Figure 6-22 Connection diagram 11
1 2 3 4 5 6 7 8
Slip ring
BA40-20-
Heat_dish3 White-purple 18
Heat_dish2 White-green 16
Heat_dish1 White-orange14
PT3_D_I White-black 11
PT3_S_I White-brown12
+24V White-blue 17
+24V White-yellow 15
PT3_S_O White 10
14 GND White-red 13
PT1_D_O Black 1
PT1_S_O Brown 2
Red 3
PT1_S_I Orange 4
PT2_D_O Yellow 5
PT2_S_O Green 6
Blue 7
Purple 8
Gray 9
D D
BA40-21-61677
BA40-21-61676
To shield
PT3_D_O
PT1_D_I
PT2_D_I
PT2_S_I
NC
BA 40-21-61766 BA40-21-61757
10
11
12
5
6
4
3
4
5
1
2
2
3
7
8
1
1 SIG N Red 1 V CC 1red
2 SIG N 2 A W _PHO 4whiter +
C W ash unit up /dow n sensor
3 SIG N 1 2 3 4 5 6 7 3 GND 2black - B A40-21-
J50 1 2 3 4 5 6 4 GND 3green
4 SIG N J2 E
5 G ND 8 9 10 11 12 13 14
5 V CC 1red +
2 1 6 SIG N 2 1 6 AW _V _PH O 4whiter W ash unit collision sensor
J10 2black C (H )
4 3
7 SIG N
4 3 J23 1 2 7 GND -
8 GND 3green B A40-21-
8 SIG N E
6 5 9 SIG N 6 5 3 4
9 VC C 1red +
8 7
10 SIG N
8 7 5 6 10 R T _PHO 4whiter C R eagent disk hom e position
11 SIG N
7 8 11 GND 2black - sensor
10 9 12 SIG N 10 9 12 GND 3green E B A40-21-
13 SIG N
12 11 9 10
12 11 14 SIGN 13 VC C 1red
C 11 12 14 RTC _PH O 4whiter + C
14 13 15 G ND 14 13 C Reagent disk coder sensor
15 GND 2black -
16 SIGN
16 15 13 14 16 GND 3green
BA40-21-
16 15 17 SIGN
18 SIGN 18 17 J26 15 16 1red E
18 17 17 VC C
+
19 SIGN 17 18 18 ST _PHO 4whiter
20 19 20 19 19 2black C Sam ple disk hom e position
20 G N D GND
3green - sensor
19 20 20 GND E BA 40-21-
21 SIG N 22 21
Pump/valve drive
22 21
BA40-30-61373
22 SIG N 21 22
24 23 1red
24 23 23 SIG N Three-disk drive board 23 24
21
22
VC C
STC _PH O 4whiter +
26 25 24 SIG N 26 25 23 2black C Sam ple disk coder sensor
B A 40-30-61359 GND
board
25 26 - B A40-21-
25 GND 24 GND 3green E
28 27 26 SIG N 28 27
27 28 1red
30 29 27 SIG N 30 29 25 VC C
+ R eaction disk hom e position
28 SIG N 29 30 26 R EA C _PH O 4whiter C
27 2black sensor
32 31 29 SIG N 32 31 GND -
31 32 28 GND 3green E
BA 40-21-
30 GND
34 33 34 33
31 V CC 33 34 29 VC C 1red
30 REA CC _PHO 4whiter +
32 V CC C
33 V CC 31 GND 2black -
Reaction disk coder sensor
32 GND 3green E
B A40-21-
34 V CC
33 NC
34 NC
J22 1 V CC Red
J27
B B
2 SIG N
1 2 1 2 1 SE N_W 1+ 1red W ash solution tem p. sensor 1
3 SIG N
2 SEN _W 1- 2white B A40-21-
3 4
4 SIG N
3 4 1 2
5 SIG N 3 SE N_W 2+ 1red
3 4 W ash solution tem p. sensor 2
5 6 6 GND 5 6 J20 4 SEN _W 2- 2white
J9 BA 40-21-
7 SIG N J31 5 6 5 1red
7 8 7 8 SE N_W 3+
8 SIG N 6 SEN _W 3- 2white W ash solution tem p. sensor 3
9 10 9 SIG N 9 10 1 2 3 4 5 6 2 4 6 8 10 7 8 BA 40-21-
7 G N D to shield
10 SIG N 1 3 5 7 9 8 NC
7 8 9 10 11 12
BA 40-21-61767
BA40-21-61770
MINDRAY
CA _W _2
6 VC C 2white W ash solution heater 2 BA40-21-61769
BA40-21- 5 SIN G 1red
6 G ND 2black concentrated bottle level sense
A 7 VC C 1black BA 40-21- A
8 VC C 2green W ash solution heater sw itch 2
Three-disk drive board
BA 40-21- 7 SIN G 1red C oncentrated detergent B bottle level TITLE:
9 CA _W _3 1red
8 G ND 2black sense
BA 40-21-
connecting diagram 2
File: Bytes: 2white
10 VC C W ash solution heater 3
B A 40-21- 9
10
SIN G
G ND
1red C oncentrated detergent A bottle DWG NO. A-B A40-30- REV 1.0
Date: Time: 2black level sense
11 VC C 1black BA 40-21-
12 VC C 2green W ash solution heater sw itch 3
Software & Rev: Microsoft office Visio 2003
B A 40-21-
SHEET 11 OF 16 SIZE A3
1 2 3 4 5 6 7
6 Hardware 6-25
Figure 6-23 Connection diagram 12
1 2 3 4 5 6 7 8
BA 40-20-
D 1 red VCC
Reaction disk heater 1 D
J5
BA40-30- 2 Black GND
2
BA40-21-61672 BA40-21-61675
3 4 6 Green PT2_S_O 6
BA40-21-61672
Slip ring connection board 5 6 7 Blue PT2_D_I 7
1 red PT1000 power out
1 BA40-30-61375
7 8 8 Purple PT2_S_I 8
C C
2 Black PT1000 sense out
2
Tem perature sensor J2 9 10 9 Gray PT3_D_O 9
Slip ring
3 Yellow PT1000 pow er In
BA40-
BA40-30- 3 10 W hite PT3_S_O 10
11 12
4 Green PT1000 sense In
4 11 W hite-black PT3_D_I 11
13 14
12 W hite-brown PT3_S_I 12
15 16
BA40-21-61672 13 W hite-red GN D 13
17 18
14 NC
1 red PT1000 power out
1 19 20
2 Black PT1000 sense out 15 NC
2
Tem perature sensor 16 W hite-orange Heat_dish1 14
3 Yellow PT1000 power In J3
BA40-30- 3
17 W hite-yellow +24V 15
4 Green PT1000 sense In
4
18 W hite-green Heat_dish2 16
19 W hite-blue +24V 17
B BA40-21-61673 B
Ports: J1 ~ J9 20 W hite-purple Heat_dish3 18
A
MINDRAY A
Slip ring connection board
TITLE:
connecting diagram
File: Bytes:
DWG NO. A-BA40-30- REV 1.0
Date: Time:
6-26 6 Hardware
Figure 6-24 Connection diagram 13
1 2 3 4 5 6 7 8
Heat sensitivity resistor Peltier cooler Peltier cooler Peltier cooler Peltier cooler
3001-21-07100 BA30-10-06633 BA30-10-06633 BA 30-10-06633 BA30-10-06633
D D
2 black
2 black
2 black
1 red
2 black
1 red
1 red
2 black
1 red
1 red
BA40-20-61656
BA40-20-61648
BA40-20-61656
BA40-20-61656
BA40-20-61656
Control
Control
Control
VCC
Control
VCC
VCC
VCC
2 black
2 black
2 black
1 red
2 black
1 red
1 red
2 black
1 red
1 red
BA40-20-61908
BA40-20-61586 1 2 1 2 1 2 1 2 1 2
J7
connection board
BA40-30-61781
J1
Vacuum pump
J3 J4 J5 J6
1 black GND 1 black
1 24V Red 1 1
1 1 J7
2 FGND Black 2 red VCC 2 red
2 2 2
C 2 J17 Ports: J1 ~ J21, P1, P2 C
J9 Yellow
3 12VFAN
3 3
Reserved ports:
BA40-30-61627
Power module
4 SGND Green
4 4
Reagent refrigeration board J8, J9, J10, J11
1 12V Red 1 BA40-30-61371
1 J12, J13, J14
P1
2 12V Red 2
2 J22
Three-disk drive
BA40-30-61359
1 black GN D 1 black
1 1
board
3 GND Black 1
1
P2 2 yellow SIGN 2 yellow
4 GND Black 2 J19 2 2
2
3 red VCC 3 red
3 3
1 2 3 1 2 1 2 1 2 1 2 1 2
BA 40-20-61909
B B
2 yellow
1 black
1 black
1 black
1 black
1 black
1 black
2 red
3 red
2 red
2 red
2 red
2 red
BA40-20-61652
BA40-20-61654
BA40-20-61654
BA40-20-61644
BA40-20-61646
BA40-20-61650
DATA
VCC
GND
GND
GND
GND
GND
GND
VCC
VCC
VCC
VCC
VCC
2 yellow
1 black
1 black
1 black
1 black
1 black
1 black
2 red
3 red
2 red
2 red
2 red
2 red
Cool fan Lam p fan PCB cooling fan PCB cooling fan Defog heater Defog temperature switch
BA 40-20- M 07-00062S--- 2100-20-08144 2100-20-08144 BA40- BA40-
A
MINDRAY A
Reagent refrigeration board
TITLE: connecting diagram
File: Bytes:
DWG NO. A-BA40-30- REV 1.0
Date: Time:
6 Hardware 6-27
Figure 6-25 Connection diagram 14
1 2 3 4 5 6 7 8
BA40-21-61766
D D
BA40-21-61747
1 SIGN Red
1 +5V Red 2 SIGN
J22 3 SIGN
2 GND Black
J31 3 R1_VA LUE_OUT Green J2 4 SIGN J2
4 GND Black 1 2 5 GND
1 2 1 2 6 SIGN 1 2
5 R2_VA LUE_OUT Green
3 4
3 4 6 GN D Black 3 4 7 SIGN
3 4
Three-probe drive board
Three-disk drive
BA40-30-61359
23 24 22 SIG N
23 SIG N 23 24
C 25 26 24 SIG N C
25 26
board
27 28 25 GN D
26 SIG N 27 28
29 30 27 SIG N 29 30
31 32 28 SIG N
Ports: J1 ~ J50 29 SIG N 31 32
33 34 30 GND
33 34
31 V CC
J4 1 +12V Reserved ports: 32 V CC
2 GND J1
33 V CC
1 2 3 +12V J18~J21, J23~J25
1 2
BA40-30-61627
34 V CC
Power module
4 GND
3 4 5 +12V 3 4 J31~J43, J46
5 6 6 GND
7 +12V 5 6 J50 J27
1 V CC R ed
7 8 8 GND 7 8 2 SIG N
9 10 9 +24V 1 2 1 2
10 GN D 9 10 3 SIG N
4 SIG N
11 12 11 +24V 3 4 3 4
11 12 5 SIG N
12 +24V 5 6 6 GND 5 6
7 SIG N 7 8
7 8
8 SIG N
BA40-20-61807 9 10 9 SIG N 9 10
J47 J48 J49 J44 J45 10 SIG N
B
B
1 2 3 4 1 2 3 4 1 2 3 4 1 2 1 2
3yellow
3yellow
BA40-21-61767
3yellow
SENSOR_LIQUID_2 4green
SENSOR_LIQUID_2 4green
SENSOR_LIQUID_1 2black
SENSOR_LIQUID_1 2black
SENSOR_LIQUID_2 4green
SENSOR_LIQUID_1 2black
2black
2black
1red
1red
1red
1red
1red
BA40-21-61687
BA40-21-61689
BA40-21-61691
BA40-21-61683
A40-21-61685
SENSOR_LIQUID
SENSOR_LIQUID
GND
GND
GND
GND
GND
GND
GND
GND
Diluted wash solution A D iluted wash solution B C oncentrated wash solution
W ater tank liquid level tank level sensor tank level sensor W aste tank level sensor
tank level sensor
BA 40-20-
MINDRAY
sensor BA40-20- BA40-20- BA40-20-
BA 40-20-
A A
Pum p/valve drive board
TITLE: connecting diagram
File: Bytes:
DWG NO. A-B A40-30- REV 1.0
Date: Time:
6-28 6 Hardware
Figure 6-26 Connection diagram 15
1 2 3 4 5 6 7 8
BA 40-21-61681
BA40-21-61681
BA 40-21-61681 BA40-21-61681
BA 40-21-61681 BA40-21-61681
BA 40-21-61681 BA40-21-61681
Pump/valve drive board
Phase 2 washing 1 Black VLAUE BA40-30-61373 1 VLAUE First reagent probe
1 1
control valve 2 +12V J8 outside wash valve
Red J26 2 +12V
BA40-30- 2 2 BA40-30-
BA 40-21-61681 BA 40-21-61681
Phase 3-6 washing 1 Black VLAUE 1 VLA UE Sam ple probe outside
1 1
control valve J9 wash valve
2 Red +12V J27 2 +12V
BA40-30- 2 2 BA40-30-
B BA40-21-61681 BA40-21-61681 B
W ash solution B 1 Black VLAUE VLAUE
1 1
1 Sam ple m ixer wash
control valve J10
2 Red +12V J28 2 +12V valve
BA40-30- 2 2 BA40-30-
BA40-21-61681
BA40-21-61681
Phase 1 washing 1 Black VLAUE 1 VLA UE Reagent m ixer wash
1 1
control valve J11 valve
2 Red +12V J29 2 +12V
BA40-30- 2 2 BA40-30-
BA40-21-61681
BA40-21-61681
MINDRAY
BA40-30-
A A
6 Hardware 6-29
Figure 6-27 Connection diagram 16
1 2 3 4 5 6 7 8
D D
BA40-21-61701
NC
1
J1
3 Blue Line 2 Blue
2 BA40-21-61704
BA40-21-61702 BA40-20-61910
Vacuum pump 1 Black GND
Vacuum pump connection board
1
cooling fan J8 BA40-30-61781 1 AC1
2 Red VCC
3100-20-49437 2 2
J13
1 5 3 AC2 1 5
BA40-30-61627
Power module
BA40-21-61703
2 6 4 2 6
1 Red VCC J5
Pressure 1 3 7 5 3 7
protection switch J4
2 Black GND
2 6 ACINN
4 8 4 8
7 B
B
8 ACINN
BA40-20-61908
1 1 Black GND
1
J7 J7
2 Red VCC
2 2
Reagent
refrigeration board
BA40-30-61371
A
MINDRAY A
6-30 6 Hardware
7 Replacement of Other
Components and Parts
7.1 Overview
Tools used for replacement:
Hex wrench
Cross-head screwdriver
Adjustable wrench
BA40-J18-1
Blade
Seal tape
Securing methods:
Latch hooks
Retaining screws
Right panel
Installation site
of right panel
Retaining screws
Securing methods:
Latch hooks
Retaining screws
Securing methods:
Hinges
Front panel
Securing methods:
Rear panel
Securing methods:
Retaining screws.
Clean the vacuum tank cover and sealing ring; place the sealing ring in the groove of
the tank opening; use the fixture(BA40-J35-1) to tighten the tank cover.
Seal the vacuum tank properly; otherwise air leakage may occur.
Pressure tank
Clean the pressure tank cover and sealing ring; place the sealing ring in the groove of
the tank opening; use the fixture(BA40-J35-1) to tighten the tank cover.
Seal the pressure tank properly; otherwise air leakage may occur.
Floater sensors
Water tank
Clean the water tank cover and sealing ring; place the sealing ring in the groove of
the tank opening; use the fixture(BA40-J35-1) to tighten the tank cover.
Seal the water tank properly; otherwise air leakage may occur.
Waste tank
NOTE:
When the washing of the waste tank is completed and you are about to
reassemble the it, please make sure the O-ring should be placed at the
right position on the mouth of the tank and be placed smoonthly to avoid
leakage after installation.
Clean the diluted wash solution tank cover and sealing ring; place the sealing ring in
the groove of the tank opening; use the fixture(BA40-J18-1) to tighten the tank cover.
Seal the diluted wash solution tank properly; otherwise air leakage may occur.
Use two M3X5 cross pan head screws with washer to respectively fix the 6 solenoid
valves 2 to the left bracket.
Use two M3X8 cross pan head screws with washer to respectively fix the 3 solenoid
valves 3 to the right bracket.
Precautions:
The valves SV08/SV09/SV10/SV28 have their outlets located on the silk side of the
left bracket and their inlets on the silk side of the right bracket.
Use two M3X5 cross pan head screws with washer to respectively fix the eleven
solenoid valves to the right bracket.
1. Use four M4x10 socket head screws with washer to fix the drive part to the
2. Screw the T-piece to the syringe manually until secure. Be sure to place a plastic
washer between the T-piece and the syringe.
3. Use retaining screws to fix the drive module and its bracket to the framework of
the analyzer.
4. Install the syringe on the V-bracket, ensuring the upper end of the V-bracket
reach the 11th scale on the syringe; then use two space bars and four retaining
screws to fix the syringe on the V-bracket.
5. Rotate the syringe motor and tighten the lower retaining screw of the plunger
while the plunger moves downwards.
Precautions:
2. While fixing the retaining screws, be sure to tighten them alternately with
equilibrium force.
1. Use four M4x10 socket head screws with washer to fix the drive part to the
syringe bracket.
2. Screw the T-piece to the syringe manually until secure. Be sure to place a plastic
washer between the T-piece and the syringe.
3. Use retaining screws to fix the drive module and its bracket to the framework of
the analyzer.
5. Rotate the syringe motor and tighten the lower retaining screw of the plunger
while the plunger moves downwards.
Precautions:
1. The T-piece of reagent syringe differs from that of sample syringe in the upper
threaded hole, which is located on the top of the T-piece. Tighten properly the
T-piece to the reagent syringe and ensure that the threads on the syringe are not
damaged.
2. While fixing the retaining screws, be sure to tighten them alternately with
equilibrium force.
To ensure reliability, good performance and service life of the system, regular
maintenance is required. Be sure to follow the instructions given below to maintain
the system. Even you’re only an operator, it’s very important for you to learn this
chapter. Your thorough understanding will help you obtaining the best performance of
the system.
WARNING
Do not perform any maintenance procedures that are not described
in this chapter.
Do not touch the components other than the ones specified in this
chapter.
Performing unauthorized maintenance procedures may damage your
system, void any applicable warranty or service contract and even
cause personal injury.
After performing any maintenance actions or procedures, ensure that
the system runs normally.
Do not spill water or reagent on mechanical or electrical components
of the system.
BIOHAZARD
Wear gloves and lab coat and, if necessary, goggles during
maintaining process.
8.1 Preparation
The following tools, wash solution and ethanol may facilitate your maintenance.
WARNING
Poisonous gas will be produced if acid wash solution is mixed with
alkaline wash solution. Do not mix the acid wash solution with the
alkaline one.
CAUTION
Mindray has specified the following enhanced wash solutions:
Acid wash solution: 0.1mol/l hydrochloric acid;
Alkaline wash solution: javel water with 0.5% active chlorine..
Be sure to use the enhanced wash solution specified by Mindray.
Otherwise, proper result may not be obtained.
Mindray recommends the acid and alkaline wash solutions be used
alternately. For instance, if the acid wash solution is used at current
startup, the alkaline one should be used at next startup.
8.1.3 Others
Water-free ethanol
Disinfectant
CAUTION
Make sure the tubing in the hydropneumatic drawer is neither clogged
nor bent.
BIOHAZARD
To prevent biohazard contamination, always wear gloves and lab coat
and, if necessary, goggles when checking the waste tubing.
Check if the waste drainage system works normally. Ensure the waste tubing is
neither bent nor clogged, and the high-/low-concentration waste is handled properly
according to local regulations and rules for waste disposal.
CAUTION
Ensure the waste tubing is neither clogged nor bent. Clogged or bent
waste tubing may lead to waste overflow that can damage your analyzer.
3 If the vacuum/pressure readings are incorrect, adjust the 5psi and 10psi
gauges for pressure regulating valves and adjust the 25psi gauge for the
air pump. Make sure the 25psi gauge is prior.
4 Close the front doors of the analyzer.
BIOHAZARD
To prevent biohazard contamination, always wear gloves, goggles and
protective clothing when doing the below checks.
The cleaning solution is irritating to eyes and skin. Avoid contact with
skin and eyes. In case of contact with eyes, rinse immediately with
plenty of water and seek medical advice.
CAUTION
Use the consumables recommended by Mindray. Other consumables
may degrade system performance.
Add solution supplied in the cleaning solution kit to top of label on the
powder bottle that is also supplied in the same kit and shake well to
prepare the cleaning solution.
The cleaning solution must be stored at 2-8°C and discarded after two
weeks.
WARNING
The sample probe tip is sharp and can cause puncture wounds. To
prevent injury, exercise caution when working around the sample probe.
BIOHAZARD
Wear gloves and lab coat and, if necessary, goggles.
Dispose of the used gauze in accordance with your local or national
guidelines for biohazard waste disposal.
3 Pull the sample probe arm to the highest point, then rotate the arm to move
the sample probe to a position above the sample compartment and
convenient to operate.
4
CAUTION
Do not contact the sample probe directly with tweezers;
otherwise the sample probe may be scratched. Excessive
force may bend the sample probe.
NOTE
Mindray recommends the acid and alkaline wash solution be
used alternately for this purpose. For instance, if the acid
wash solution is used for last maintenance, the alkaline one
should be used for this time.
WARNING
The reagent probe tip is sharp and can cause puncture wounds. To
prevent injury, exercise caution when working around the reagent
probe.
BIOHAZARD
Wear gloves and lab coat and, if necessary, goggles.
Dispose of the used gauze in accordance with your local or national
guidelines for biohazard waste disposal.
4
CAUTION
Do not contact the reagent probe directly with tweezers;
otherwise the reagent probe may be scratched. Excessive
force may bend the reagent probe.
NOTE
Mindray recommends the acid and alkaline wash solution be
used alternately for this purpose. For instance, if the acid wash
solution is used for last maintenance, the alkaline one should
be used for this time.
BIOHAZARD
Wear gloves and lab coat and, if necessary, goggles.
Dispose of the used gauze in accordance with your local or national
guidelines for biohazard waste disposal.
3
CAUTION
Do not contact the mixer directly with tweezers; otherwise it
may be scratched. Excessive force may bend the mixer.
NOTE
Mindray recommends the acid and alkaline wash solution be
used alternately for this purpose. For instance, if the acid
wash solution is used for last maintenance, the alkaline one
should be used for this time.
Use acid or alkaline wash solution-dipped gauze to gently wipe the mixer
until it is clean and smooth.
CAUTION
The mixer is precisely fabricated. In case of scratched or bent mixer,
replace it according to 8.8.6 Replacing Sample/Reagent Mixers.
CAUTION
Do not stare at the laser of the bar code reader; otherwise your eyes
may get hurt.
CAUTION
Do not use sharp-edged tools to scratch the bar code reader window.
WARNING
The sample probe tip is sharp and can cause puncture wounds. To
prevent injury, exercise caution when working around the sample
probe.
BIOHAZARD
Wear gloves and lab coat and, if necessary, goggles.
Dispose of the used gauze in accordance with your local or national
guidelines for biohazard waste disposal.
3 Rinse the sample disk with fresh water and dry it with gauze.
4 Wipe the inside of the sample compartment using clean gauze or, if
necessary, the gauze dipped with water or disinfectant.
5 Install the sample disk and tighten the two retaining screws on it. Then
cover the sample compartment.
WARNING
The reagent probe tip is sharp and can cause puncture wounds. To
prevent injury, exercise caution when working around the reagent
probe.
BIOHAZARD
Wear gloves and lab coat and, if necessary, goggles.
Dispose of the used gauze in accordance with your local or national
guidelines for biohazard waste disposal.
5 Install the reagent disk and together the screws on it. Then cover the
reagent compartment.
WARNING
The probe/mixer tip is sharp and can cause puncture wounds. To
prevent injury, exercise caution when working around the probe/mixer.
BIOHAZARD
Wear gloves and lab coat and, if necessary, goggles.
Dispose of the used gauze in accordance with your local or national
guidelines for biohazard waste disposal.
Aside from the regular check, checking should be done after the replacement of
cuvettes and lamp.
1 Enter the Daily Maint. page of the Utilities screen; then select
Cuvette/Lamp Check in the Maintenance area and click Execute.
NOTE:
To ensure the good performance of the photometer, replace
those cuvettes marked with yellow, blue or red. Run cuvette
check after replacement, and save the data.
Place DI water in position W. Click Start. After test, the cuvette status will
be refreshed according to the test result. Click Save to save the result.
NOTE:
If Save is not selcted, the current test result will not be saved.
Next time when you enter this page, the cuvette status will be the
previous test result.
NOTE:
Before running lamp check, replace those cuvettes marked as
yellow, blue and red.
Click Lamp check to enter the lamp checking page as shown in the
following figure.
Time for lamp check: 1.5min
In Lamp check page, you can view the latest two lamp check results. The
displayed value is the average of five consecutive cuvette aborsorbance.
When this value is larger than 10458, the lamp intensity is not strong
enough.
Click Start to start the lamp check. The test result and the lamp status will
be refreshed after the test. Click Save to save the result.
NOTE:
If Save is not selcted, the current test result will not be saved.
Next time when you enter this page, the lamp status will be the
previous test result.
NOTE:
To ensure the good performance of the photometer, replace the
lamp when the light intensity is not strong enough.
You can readjust the gain after replacing the lamp. When the water blank is
WARNING
Always wear gloves and lab coat and, if necessary, goggles when
checking the wash solution.
3 After all the liquid in the separators is removed, tighten the drain caps or
drain screw.
WARNING
The sample probe tip is sharp and can cause puncture wounds. To
prevent injury, exercise caution when working around the sample
probe.
BIOHAZARD
Wear gloves and lab coat and, if necessary, goggles.
Dispose of the used cotton swabs in accordance with your local or
national guidelines for biohazard waste disposal.
3 Use cotton swabs to clean the inside of and the places around the wash
well.
4 After cleaning, gently pull the probe arm to its highest point and rotate it to
move the sample probe to a position above the wash well.
5 Place the Power to ON. Wait for about 30 seconds and then execute
“System Reset” on the Daily Maint. page. The system will reset and rinse
the sample probe automatically.
WARNING
The reagent probe tip is sharp and can cause puncture wounds. To
prevent injury, exercise caution when working around the reagent
probe.
BIOHAZARD
Wear gloves and lab coat and, if necessary, goggles.
Dispose of the used cotton swabs in accordance with your local or
national guidelines for biohazard waste disposal.
3 Use cotton swabs to clean the inside of and the places around the wash
well.
4 After cleaning, gently pull the probe arm to its highest point and rotate the
arm to move the reagent probe to a position above the wash well.
5 Place the Power to ON. Wait for about 30 seconds and then execute
“System Reset” on the Daily Maint. page. The system will reset and rinse
the reagent probes automatically.
WARNING
The mixer tip is sharp and can cause puncture wounds. To prevent
injury, exercise caution when working around the mixer.
BIOHZARD
Wear gloves and lab coat and, if necessary, goggles.
Dispose of the used cotton swabs in accordance with your local or
national guidelines for biohazard waste disposal.
3 Use cotton swabs to clean the inside of and the places around the wash
well.
4 After cleaning, gently pull the mixer arm to its highest point and rotate the
arm to move the mixer to a position above the wash well.
5 Place the Power to ON. Wait for about 30 seconds and then execute
“System Reset” on the Daily Maint. page. The system will reset and rinse
the mixers automatically.
WARNING
The sample probe tip is sharp and can cause puncture wounds. To
prevent injury, exercise caution when working around the sample
probe.
BIOHAZARD
Wear gloves and lab coat and, if necessary, goggles.
WARNING
The reagent probe tip is sharp and can cause puncture wounds. To
prevent injury, exercise caution when working around the reagent
probe.
BIOHAZARD
Wear gloves and lab coat and, if necessary, goggles.
Dispose of the used cotton swabs in accordance with your local or
national guidelines for biohazard waste disposal.
WARNING
The mixer tip is sharp and can cause puncture wounds. To prevent
injury, exercise caution when working around the mixer.
BIOHAZARD
Wear gloves and lab coat and, if necessary, goggles.
Dispose of the used cotton swabs in accordance with your local or
national guidelines for biohazard waste disposal.
3 Rub the wipe blocks with gauze soaked with DI water until the surface of the
wipe block is clean.
CAUTIONS:
Exercise caution when cleaning the wipe block. Excessive
force may deviate the angel of the wipe block. If that
happens, you should readjust it.
4 Reinstall the wash unit to the holder. The two guide pins on the holder should
be inserted into the guide holes of the wash unit. Fasten the retaining screws
manually.
Guide
pin of
the
wash
unit
Guide
hole of
the
wash
unit
The
thinner
part of the
wipe
blocks
should
face
outward.
4 After alignment, manually insert the wash station wipe blocks into the cuvettes.
Check whether the wipes blocks fits into the cuvettes and whether the wipe
blocks are level to the cuvette walls.
BIOHAZARD:
Wear gloves and lab coat and, if necessary, goggles.
Dispose of the damaged cuvette in accordance with your local or
national guidelines for biohazard waste disposal.
CAUTION:
Please use our recommended consumables. Other consumables may
degrade the system performance.
CAUTION:
Do not drag the optical fibire under the raction disk.
Do not drag
the optical
fibire
CAUTION:
The optical surface of the cuvette should be perpendicular to
the radial direction of the reaction disk.
Press the cuvettes into the bottom of their position on the
disk until no further can be pushed downward.
Do not touch the optical surface of the cuvettes.
7 Install the bullets
CAUTION:
Check for cuvettes and bullets that are forgotten to be
installed.
8 Install the reaction disk back.
Hold the screen with your hands and lift it upwards, then remove it
outwards.
4 Rinse the dust screen with fresh water and dry it by air.
5 Install the dust screens correctly.
6 Close the front doors of the analyzer.
1 Place the MAIN POWER to OFF. Wait at least 15 minutes for the lamp and
its housing to cool down.
WARNING:
After working for a while, the lamp and its housing are usually
hot enough to burn you. Do not proceed with this procedure
until they have cooled down.
2 The light source assembly is at the right rear part of the instrument.
Unscrew the two retaining screws on the back cover by using a screw
driver. Loosen the two retaining screwers on the upper part. Remove the
back cover.
3 Unplug the fan power cable from the socket. Uncscrew the two air duct
retaining screws by using a screw driver. Pull the air duct out.
Fan power
cable
Air duct
retaining
screws
Radiator plate
retaining
crews
5 Please wear the glove to protect the circuit boards from ESD. Press the
lever and then pinch the lamp base and pull out the lamp.
Lever
NOTE:
The larger pin is inserted into the larger hole and the smaller
pin into the smaller hole. The lamp should be pressed to the
bottom of the mounting hole. Otherwise, performance wil be
affected.
Don’t pinch the bulb of the lamp so that the lamp will not be
contaminated or broken.
7 Install the lamp housing radiator back. Tighten the screws at the lower part
of the housing.
8 Install the air duct back and tighten the retaining screws. Reconnect the fan
power cable.
9 Close the back cover of the analyzing unit and tighten the four retaining
screws.
1 Turn off the ball valve on the hydropneumatic drawer. The water supply
module is powered off.
2 Turn on the ball valve on the water supply module to release the remaining
pressure. When the pressure gauge indicates 0, turn off the ball value.
3 Press the tubing release button to remove the tubing 91 and 92 from two
ends of the old filter assembly. (If these tubings are dirty, replace them)
4 Connect the tubing 91 and 92 to the two ends of the new filter assembly.
5 Power on the water supply module, turn on the ball valve on it and wait for
5 minutes. When you see the VENT of the water supply module is
supplying water continuously which sigifies the normal working of the
module, turn off its ball valve.
6 Turn on the ball valve on the hydropneumatic drawer.
The other two filters are at the left side of the drawer, at the end of the dilute wash bottle
A, B tubings, at the joints of D09and D10, D04 and D05.
3 After replacing the new filter, reconnect the tubings. If you discover the end of the
tubing is distorted, use sccissors to cut a small part of the tubing and then reconnect
the tubings to ensure good connection. You do not have to pay attention to the direction
of the tubing, while mounting the filter.
4 Check the conncection of the tubings and whether leakage happens at the adapters.
NOTE:
While replacing the check valves, pay attion to their directions.
After replacement, the 9 check valves should meet the
following requirements.
Behind SV16 and SV17, at the joints of D15 and D16, D20
and D21.(2): the side with the diaphram should be connected
with D15, D20. The flow direction in the tubings should be:
D15→D16, D20→D21.
On the the inlet tubings of dilute wash bottle A, B , at the joints
of D02 and D02, D07 and D08. (2): the side with the
diaphram should be connected with D15, D20. The flow
direction in the tubings should be: D02→D03, D07→D08.
on the the outlet tubings of dilute wash bottle A, B , at the joints
of D 10 and D11, D05 and D22. (2): the side with the
diaphram should be connected with D10, D05. The flow
direction in the tubings should be: D10→D11, D05→D22.
on the tubing of degassing disphram gas-passing end, at the
joints of C50 and C23.(1): the side with the diaphram should
be connected with C50. The flow direction in the tubings
should be: C50→C23.
on the DI water outlet tubing of degassing disphram. (1): the
side with the diaphram should be connected with J04. The flow
direction in the tubings should be: J04→J05.
on the waste outlet tubing of drying module. (1): the side with
the diaphram should be connected with W24. The flow
direction in the tubings should be: W24→W25.
The flow direction of the liquid in the check valve is shown in the following figure.
The flow direction of the liquid in the check valves of the 1-2 dispensing tubing is
shown in the following figure.
The flow direction of the liquid in the check valves of the 3-6 aspiration tubing is
shown in the following figure.
NOTE:
While replacing the check valves, pay attion to their directions.
4 Check the conncection of the tubings and whether leakage happens at the
adapters.
8.8
As-Needed Maintenance
8.8.1 Unclogging Sample Probe
When the sample probe is clogged, the fluid flow will become abnormal. Follow this
procedure to remove, unclog and install the sample probe.
WARNING
The sample probe tip is sharp and can cause puncture wounds. To
prevent injury, exercise caution when working around the sample probe.
3 Pull the probe arm to the highest point, then rotate the arm to move the
sample probe to a position above the sample compartment and convenient
to operate.
4 Grab the lower part of the arm cover with your hands and pull them slightly
outwards and then remove the cover upward from the arm base.
CAUTION
Exercise caution when disconnecting the connector.
Excessive force may damage the connector and/or the circuit
board.
NOTE
Exercise caution when pulling the sample probe away from
the arm so that the probe tip will not contact or even damage
the probe arm.
Slowly pull the probe away from the probe arm. Exercise caution so that
the gasket inside the probe does not drop out and if it does, store it in a
clean place for later installation. Replace the gasket if it has been
disassembled for 2 to 3 times. Otherwise leakage may occur or sampling
precision be affected.
NOTE
The sample probe is precisely fabricated for accurate
aspiration/dispensing. In case of scratched or bent sample probe,
replace it.
WARNING
The sample probe tip is sharp and can cause puncture wounds. To
prevent injury, exercise caution when working around the sample probe.
BIOHAZARD
Wear gloves and lab coat and, if necessary, goggles.
Dispose of the used needle in accordance with your local or national
guidelines for biohazard waste disposal.
CAUTION
The sample probe is precisely fabricated for accurate
aspiration/dispensing. In case of scratched or bent sample probe,
replace it.
WARNING
The sample probe tip is sharp and can cause puncture wounds. To
prevent injury, exercise caution when working around the probe.
BIOHAZARD
Wear gloves and lab coat and, if necessary, goggles.
3 Sleeve the spring on the rotor and screw the retaining screw to secure.
4 Pinch the sample probe by the part near the probe arm. Gently push the
probe upward and then release it to see if the spring can move freely.
CAUTION
Exercise caution when connecting the sample probe.
Excessive force may bend the probe.
If not, it means the liquid level detection is failed. Check the liquid level
detection board and its connectors, and adjust the voltage on the board if
necessary.
9 Install the probe arm and make sure it is clicked properly into the arm
base.
10 Pinch the sample probe by the part near the probe arm. Gently push the
probe upward and then release it to see if the spring can move freely.
If yes, proceed to the next step.
If not, check for errors and try again after removing the errors.
11 Gently pull the probe arm to its highest point and rotate it to move the
probe to a position above the wash well.
CAUTION
After installation, be sure to move the sample probe to a
position above its wash well.
CAUTION
The sample probe is precisely fabricated for accurate
aspiration/dispensing. In case of scratched or bent sample probe,
replace it.
BIOHAZARD
Wear gloves and lab coat and, if necessary, goggles.
WARNING
The reagent probe tip is sharp and can cause puncture wounds. To
prevent injury, exercise caution when working around the reagent
probe.
BIOHAZARD
Wear gloves and lab coat and, if necessary, goggles.
CAUTION
Exercise caution when disconnecting the connector.
Excessive force may damage the connector and/or the circuit
board.
NOTE
Exercise caution when pulling the probe away from the arm
so that the probe tip will not contact or even damage the
probe arm.
Slowly pull the probe away from the probe arm. Exercise caution so that
the gasket inside the probe does not drop out and if it does, store it in a
clean place for later installation. Replace the gasket if it has been
disassembled for 2 to 3 times. Otherwise leakage may occur or sampling
precision be affected.
NOTE
The reagent probe is precisely fabricated for accurate
aspiration/dispensing. In case of scratched or bent reagent probe,
replace it.
WARNING
The reagent probe tip is sharp and can cause puncture wounds. To
prevent injury, exercise caution when working around the reagent
probe.
BIOHAZARD
Wear gloves and lab coat and, if necessary, goggles.
Dispose of the used needle in accordance with your local or national
guidelines for biohazard waste disposal.
CAUTION
The reagent probe is precisely fabricated for accurate
aspiration/dispensing. In case of scratched or bent reagent probe,
replace it.
WARNING
The reagent probe tip is sharp and can cause puncture wounds. To
prevent injury, exercise caution when working around the reagent
probe.
BIOHAZARD
Wear gloves and lab coat and, if necessary, goggles.
CAUTION
Exercise caution when connecting the sample probe.
Excessive force may bend the probe.
CAUTION
After cleaning, be sure to move the reagent probe to a
position above its wash well.
CAUTION
The reagent probe is precisely fabricated for accurate
aspiration/dispensing. In case of scratched or bent reagent probe,
replace it.
WARNING
The sample probe tip is sharp and can cause puncture wounds. To
prevent injury, exercise caution when working around the sample
probe.
BIOHAZARD
Wear gloves and lab coat and, if necessary, goggles.
BIOHAZARD
Dispose of the bent or damaged sample probe in accordance
with your local or national guidelines for biohazard waste
disposal.
CAUTION
After installing the sample probe, be sure to rotate it to a
position above the wash well prior to sample disk installation.
WARNING
The sample probe tip is sharp and can cause puncture wounds. To
prevent injury, exercise caution when working around the sample
probe.
BIOHAZARD
Wear gloves and lab coat and, if necessary, goggles.
WARNING
The reagent probe tip is sharp and can cause puncture wounds. To
prevent injury, exercise caution when working around the reagent
probe.
BIOHAZARD
Wear gloves and lab coat and, if necessary, goggles.
CAUTION
Please use Mindray-recommended consumables. Other consumables
may degrade the system performance.
BIOHAZARD
Dispose of the bent or damaged reagent probe in accordance
with your local or national guidelines for biohazard waste
disposal.
CAUTION
After installing the reagent probe, be sure to rotate it to a
position above the wash well prior to reagent disk installation.
WARNING
The mixer tip is sharp and can cause puncture wounds. To prevent
injury, exercise caution when working around the mixer.
When replacing, pinch the mixer only by its knurled part. Protect the
flat part of the mixer from scratches.
BIOHAZARD
Wear gloves and lab coat and, if necessary, goggles.
Dispose of the damaged mixer in accordance with your local or
national guidelines for biohazard waste disposal.
Pinch the mixer by the knurled part and unscrew counter-clockwise the
retaining nut until the mixer looses. Pull the mixer downward to remove it
and remove the nut.
6 Pinch the mixer by the knurled part and align the nut hole to the axis of the
mixer and push the nut onto the mixer until it reaches the end of the mixer.
Tighten the nut by screwing it clockwise.
CAUTION
When trying to push the mixer into the arm, concentrate your
force in the direction of the axis on the mixer arm. Biased
force may damage the mixer and/or the axis.
Ensure the mixer is all the way pushed to the end.
7 After replacing the bar, visually check whether the mixer is vertical to the
bar arm.
If not, return to step 5 to remove the mixer and reinstall it.
If yes, proceed to the next step.
8 Pull the mixer arm to its highest point and rotate it back to a position above
its wash well.
CAUTION
After installing the mixer, be sure to rotate it to a position
above its wash well.
9 Place the Power to ON. Wait for about 30 seconds and then execute
“System Reset” on the Daily Maint. page. The system will reset and rinse
the mixers automatically.
CAUTION
Please use Mindray-recommended consumables. Other consumables
may degrade the system performance.
Exercise caution when installing the plunger assembly. Excessive force
may crack the syringe.
Always wear gloves while replacing the syringe plunger assembly.
Plunger assembly of sample syringe can be replaced in the same way as that of
reagent syringe. Perform the following steps to replace the reagent syringe plunger
assembly.
Grab the T-piece with one hand and the syringe connector with the other
hand and unscrew counter-clockwise the syringe. Exercise caution so that
the gasket on the syringe does not drop out and if it does, store it in a clean
place for later installation. Replace the gasket if it has been disassembled for
2 to 3 times. Otherwise leakage may occur or sampling precision be
affected.
Unscrew counter-clockwise the plunger guide cap, pinch the plunger button
and gently pull the plunger assembly from the syringe.
8
CAUTION
The plunger rod of the syringe is slender. Exercise caution when
working on it. Excessive force may bend it.
Pinch the new plunger assembly by the plunger button and carefully insert
the plunger tip into the syringe and push it all the way to the end. Screw
clockwise the plunger guide cap until secure.
9 Immerse the syringe connector into deionized water. Pinch the plunger
button, pull it to aspirate half syringe of deionized water and then push it to
expel the deionized water and the air from the syringe.
10 Grab the T-piece with one hand and the syringe connector with the other
hand. Screw clockwise the syringe into the T-piece until secure.
11 Place the syringe on the holder.
12 Install the space bar and four upper retaining screws. Do not tighten the
screws now.
13 Align the plunger button to the lower retaining screw of the plunger and
screw clockwise the screw until secure.
14 Pinch the plunger guide cap to adjust the syringe.
15 Tighten the four upper retaining screws.
16 Place the Power to ON. Wait for about 30 seconds, then execute System
Prime on the Daily Maint. page. Repeat the instruction for several times if
necessary, and check if the T-piece is leaking.
If not, go to the next step.
If yes, tighten the syringe. If leakage remains, replace the connector and
T-piece.
17 Close the front doors of the analyzer.
CAUTION
Be sure not to push the plunger to the end tip; otherwise the
syringe may be damaged.
6 Place the syringe on the holder. Install the space bars and tighten the
upper retaining screws.
NOTE
The upper edge of the upper space bar must reach the 7th scale
on the syringe.
When fixing the retaining screws, be sure to tighten them
alternately with equilibrium force.
WARNING
The probe tip is sharp and can cause puncture wounds. To prevent
injury, exercise caution when working around the probe.
Before replacing cuvette rotate the probes to a position convenient for
operation.
CAUTION
Please use Mindray-recommended consumables. Other consumables
may degrade the system performance.
3 Use a hex wrench to loosen the three screws that fix the reaction disk.
CAUTION
Exercise caution when unplugging connectors so that the
leads and connectors will not be damaged.
6 Use your hand or tweezers to take out the damaged cuvette and then install
a new cuvette.
CAUTION
The damaged cuvette may be fixed tightly. Use tweezers to
remove the cuvette if necessary. While taking out the cuvette
using a pair of tweezers, pinch the two sides of the cuvette
simultaneously. Pinching one side of the cuvette will break the
cuvette.
Do not touch the light entrance of the new reaction cuvette, or
accurate measurement may not be achieved.
Be sure to secure the retaining spring when installing the
reaction cuvette.
7 Install the reaction disk and make sure the three screws on the reaction disk
are tightened.
8 Place the Power to ON.
9 On the Daily Maint. page, execute the Cuvette/Lamp Check instruction
and view the execution result.
If the new cuvette is still identified as damaged, check the lamp and lens,
wipe the front lens assembly with water-free ethanol and replace the lamp
and fiber if necessary.
CAUTION:
Use the consumables recommended by Mindray. Other consumables
may degrade system performance.
NOTE:
Generally after the replacement of any of the following components,
several ISE calibrations should be run before ISE Unit become stable.
WARNING:
Before performing the replacement, make sure the analyzer is
powered off.
If you run no more than 100 samples requested for the ISE tests a day, replace the
electrodes according to the following recommended schedule:
If you run more than 100 samples requested for the ISE tests a day, replace the
electrodes according to the following recommended schedule:
NOTE:
Because the electrodes must be installed sequentially, you
have to take out the electrode to be replaced and those (or
that) over it from above to below.
CAUTION:
The maintenance is necessary to be performed when the ISE unit
(optional) is connected.
The ISE unit (optional) should be on power all the time. In some cases
that the POWER will be shut down for a long time more than half an
hour, the following steps should be performed.
Use the consumables recommended by Mindray. Other consumables
may degrade system performance.
4 Select Purge Combination from the Instructions list, and enter digit “25”
in the edit boxes to the right of Purge A and Purge B. Select Execute to
start the purge cycle based on the parameters you have entered.
5 Select Maintenance Cycle from the Instructions list and select Execute.
6 Remove the electrodes.
7 Remove the Reagent Pack.
8 Put the reference, Na+, Cl- and spacer electrodes into their individual
sealed bags.
9 Aspirate a small amount of Calibrant A from the port of the reagent module
with a syringe and inject it into the lumens of the K+ electrode(and Li+
electrode) until the lumens are full.
Cover both ends of the lumens with tapes to prevent the Calibrant A flows
from the lumens.
Put the K+ electrode into their individual sealed bags.
NOTE:
The tube adapters on Reagent Pack should be covered by the red
caps. Store the Reagent Pack properly.
9.1.2 Overview
The upper area provides various function buttons to test each unit.
The lower area displays the communication data associated with the main
unit/subunits. Different colors indicate different types of data frames. The lower-right
area provides options and buttons to control the communication frame.
Disp.txt: All data frames that comply with the communication protocol.
Shakehand: Select this command to shake hand with the main unit.
System reset: Select this command to reset the subunits of the analyzer.
Unit Version: Select this command to view the versions of main unit and
subunits.
Dark Current: Select this command to inquire the dark current at each
wavelength after 2 minutes the lamp is turned off. Dark current check is often
required before the photoelectric unit and basic performance are tested.
Send instruction: Enter an instruction and select this command to send it.
Please note that this function can be performed only by debug users.
System prime: Select this command to prime the water tank. The system will
stop automatically once the water tank is full.
Sleep: Select this command to make the main unit enter sleep status.
Search Log: Select this command to view the event logs of the main unit.
To vertical home position: Select this command to move the probe/mixer to its
vertical home position.
To ver. Limit of…: Select this command to move the probe/mixer to its lowest
vertical position.
Into…: Select this command to move the probe/mixer to the fixed position of …
To liquid of …: Select this command to move the probe/mixer to the liquid level
of … or to the lowest vertical position in case of no liquid.
Syringe empty: Select this command to move the syringe to the home position.
Syringe reset: Select this command to move the syringe to the zero position and
then to the home position.
Syringe full: Select this command to move the syringe to the obverse limit
position.
Reset reagent disk: Select this command to reset the reagent disk unit.(This
command is similar to that of other units.)
Rotate to given position: Select this command to rotate the reagent disk at
specified speed for given circles and then stop it on specified position.
Find zero position: Select this option to rotate the reagent disk to the zero
position and then rotate it at specified speed for given circles and then stop it on
specified position.
Rotate obversely: Select this option to rotate the reagent disk clockwise.
Rotate for given positions: This command is similar to “Rotate to given position”
except that the reagent disk rotates for given positions and then stops.
Other commands of the sample disk unit are similar to that of the reagent disk unit.
Rotate and measure: Select this command to rotate the reaction disk at highest
speed for one circle and then stop it on position 2. During the rotating, photometric
measurement is performed.
Adjust lamp: Select this command to adjust the brightness level(0-255) of the lamp.
The two commands “Rotate to given position” and “Rotate for given positions”
are similar to that of the reagent disk unit.
Understand completely the relationship among all valves and their functions.
On the Fluidic Pressure Curve tab shows the readings of the four pressure gauges.
When all readings cannot be displayed on one screen, they will be shown circularly.
Start: Start receiving the pressure data and display it. You can control the pressure
data collecting process by using Pause, Resume and Stop.
Click any command in this area. The corresponding operation will be performed.
Manually Instruction
Multiple buttons in this area should be used during a complete test. The following
operation combinations are available:
The commands in this area can be performed circularly. On the left is the circular
command control area and on the right is the instruction area.
Note: Since the ISE module is external, a false result will be returned immediately
after an instruction is sent to it, and the true result will be returned after a period.
However, the software will start the next cycle once receiving a result. Therefore,
“Interval” should be set up carefully.
Debugging
The commands provided in this area are used to debug the ISE unit and can be
operated in the same way as the Step Instructions.
This section introduces the screen operations of the Bar Code tab.
1. Select a bar code type: Sample Bar Code or Reagent Bar Code.
Scanning test
1. Select a scan mode, which includes Dynamic, Dynamic and Static, and Static.
During scanning, you can select Pause, Resume or Stop to control the
operation.
Judge the scanning result.
All instructions that are sent or received are displayed in the box on
right-hand side of the screen. You can save or clear the instructions if
needed.
Testing scanning stability
This operation is similar to the scanning test except that the options like scan mode
and start position must not be configured.
Sending instructions
Enter the original command and select Send Instruction to send it directly.
This chapter introduces the functions of the Reaction Disk Temp. screen.
1. Set up the times of taking temperature and power readings and the interval
between two readings.
3. Select Start to start taking the temperature and power readings. During the
reading process,
You can stop or pause the taking operation, and then save and load history
readings.
You can adjust dynamically the Y-coordinate range of the temperature
curve.
You can zoom in the curve display area as needed.
The temperature data since the current startup are saved in the directory
where the test and maintenance software locates.
Checking sensor parameters and configuring the parameters
3. Select Self-adjust to notify the corresponding subunit to start adjusting the PID
parameters.
Select Heater on or Heater off. If the command is not executed correctly, select it
again.
1. Select the check box next to Reaction disk rotates and deselect Measure 90
cuvettes.
2. Set up the cuvette position to measure, times and interval for collecting data.
3. Select Start. The AD values at each wavelength for the cuvette are displayed in
tabular and graphic forms.
1. Deselect the check boxes next to Reaction disk rotates and Measure 90
cuvettes.
4. Select Start. The AD values at each wavelength for the cuvette are displayed in
tabular and graphic forms.
NOTE
After executing this command, you must reset the photoelectric unit
mechanically.
2. Set up the cuvette position to measure, times and interval for collecting data.
3. Select Start. All AD values at each wavelength for the 90 cuvettes are displayed
in tabular form.
On the left side of the screen is the Instruction area, in which you can view the
detailed instructions of specified unit. See Figure 9-7.
2. Select New.
3. Enter the name of the new macro instruction in the popup dialog box, then select
OK. The name appears in the drop-down list box next to Name.
2. Select a command.
3. Method: Select desired unit and instruction type in the Instruction area. All
qualified instructions of the unit are displayed in the lower table.
5. Enter instruction value in the Value column according to other information in the
table. Note:
Select Add to Macro. The selected command is added to the macro instruction
list on the right-hand side of the screen. Repeat steps 1) through 5) to add more
commands.
8. Select Save below the macro instruction list to save the macro instruction. The
newly-created macro instruction is temporarily stored in the memory. If not
saved, the macro instruction will disappear when you switch to other one or exit
the system.
Select a command. The table in middle of the screen shows the detailed
information of the command.
Change the Value of each byte, or reselect a unit name and instruction type to
replace the instruction of the selected No. in the macro instruction list.
4. Save the macro instruction. Select Save to save the macro instruction to the
database.
2) Enter the No. of the desired byte of the command in the Instruction Details
area.
3) Enter the delay time in the Delay field, which can be neglected if no delay is
needed before the command is executed.
4) Enter other parameters like low limit, high limit and step. In case the macro
instruction is cycled for many times, when the command is executed, the
corresponding byte of the selected No. will be increased according to the
defined low limit and step until the high limit, then the byte of the low limit (No.)
NOTE
The delay information and byte control information can exist
simultaneously or by themselves.
9.3 Performance
Most performance tests will ask you to configure a start cuvette position. However,
the cuvette on the start position you have selected may contaminate the wipe block;
therefore the system will record the cuvettes which have experienced performance
tests. When running the first performance tests since started up, the system will ask
you to select a cuvette for the performance test and then record the cuvette, which
will no longer be used for the following tests.
9.3.1.1 Introduction
Accuracy means the consistency of the measured absorbance of solution on
chemistry analyzer with the standard value.
Start reagent position is not required when Manual is selected for sampling.
4) Set up the reagent groups and sampling times for each group.
Number of reagent groups should be within 1-6, and the product of group number and
sampling times should be no greater than 90.
5) Set up the reagent volume. This option is not required when Manual is selected
for sampling.
6) Set up the primary and secondary wavelength for each reagent group. Up to 6
groups of wavelength are allowed.
Start Period means the period from which photometric measurement should be
performed since reagent is dispensed. In case of Manual sampling, the photometric
measurement is performed immediately after reagent is dispensed.
Once testing is started, the system first washes N(number of reagent groups *
sampling times for each group) cuvettes and then dispense reagents for each of them.
In Manual sampling mode, the system will remind you to dispense reagent
successively. In the start period, the system starts measuring each reaction cuvette
and takes the absorbance value of different wavelengths.
11) To review history test results, select Load. The results are displayed in the
middle of the screen.
9.3.2.1 Introduction
Stability means the difference between the maximum and minimum absorbance
values of a solution that are obtained from multiple measurements during a long time.
N(number of reagent groups) cuvettes are washed during the stability test.
The stability test allows photometric measurement to be performed when the
reaction disk is rotating or stopped.
When reagent is dispensed during stability test, the system will remind user to
add paraffin.
9.3.3.1 Introduction
Linearity test is used to measure the linearity range of the absorbance.
During the linearity test, all reagents are measured for only one wavelength.
In linearity test different items are calculated, such as blank correction,
theoretical value, etc.
9.3.3.3 Summary
The absorbance range with relative deviation less than ±5% is the linearity range.
9.3.4.1 Introduction
This test is used to determine the accuracy and precision of absorbance of diluted
samples.
4. In case of Manual dilute, you should set up the reagent position for manual
dilution(Diluted Pos.).
The system first washes N(replicates) reaction cuvettes, then the reagent
probe dispenses diluent and the sample probe dispenses sample. In the
defined start period, the system starts photometric measurement and takes
the absorbance readings.
If Manual dilute is selected, the system starts measuring the absorbance of
manually-diluted sample once auto diluted sample is analyzed. The system
again washes N(replicates) reaction cuvettes, then the reagent probe
dispenses manually-diluted solution. In the defined start period, the system
starts photometric measurement and takes the absorbance readings of the
solution.
9.3.5.1 Introduction
This test is used to determine the amount of remained water in cuvette that is washed
automatically.
2. Set up the options on the Residue of cuvette screen in the same way as the
above-mentioned tests.
3. Select Start. The system first measures the absorbance of the solution, and
washes the used cuvette, and then measure again the absorbance of the
solution.
9.3.6.1 Introduction
Sampling accuracy means the deviation between the dispensed volume by
probe(reagent probe/sample probe) and the specified volume. Sampling precision
means the deviation among multiple dispensing volumes by a probe.
9.3.7.1 Introduction
This test is to determine in which degree the sample on outside of the sample probe
may contaminate the next sample.
2. Set up the Void Times. Void Times means the number of times that the sample
probe aspirates nothing among the two types of solution.
3. Select Start. The system will remind you to add the two types of solution, and
then measure the absorbance of the solution after the sample probe aspirates
nothing for specified N(Void Times) times.
9.3.8.1 Introduction
This test is to determine how much water remains on outside of the probes and
mixers that have been washed automatically.
9.3.8.2 Operation
1. Prepare a solution for test.
2. Set up the Void Times, which means the times that the probe/mixer aspirates
nothing in each reaction cuvette.
Continuous Dilute: The probe or mixer continues with next void aspirating and
cleaning after the previous one.
9.3.9.1 Introduction
On the Stability/Drift screen absorbance range and drift can be calculated by reading
an existing photoelectric data file, and the system shows the test results for each
cuvette and enables you to learn whether the results are within specified range or not.
2. Log in the test and maintenance software; select the Performance tab; select
Stability/Drift; enter the sample ID, test No. and test date in the following fields.
3. Select the Load button. If the test data of the specified date is loaded successfully,
a prompt will pop up to indicate the successful loading.
If the test data is lost, you will be prompted to check for 90 complete test data.
4. Enter the period range to calculate the absorbance difference during maximum
reaction time.
7. Select the Result button. The list on the left side of the screen will show the
cuvettes that do not meet the criteria.
8. Select the View Graph button to review the reaction curves associated with the
test data.
9.4 Parameter
Select the Parameter from the main screen. The Parameter screen is displayed. You
can inquire and configure the parameters of the main unit and subunits. For the
probe/disk/mixer units you are allowed to set up the motor speeds. See the figure
below.
2. Select Inquire.
Configure/Configure All
1. Inquire or read the parameters of selected unit/motor.
3. Select Config. To configure the parameter of the modified line, or select Config.
All to configure all parameters of the selected unit/motor.
Save
1. Inquire or read the parameters of selected unit/motor.
Read
1. Select a target unit.
2. Select Read, then select a parameter file in the popup dialog box. If you select a
parameter file of a unit other than the target one, the latter will be replaced by
the unit specified in the parameter file.
Default
This button is similar to Save, except that when selecting Default you must not select
a target file, and the parameters will be saved in the directory where the test and
maintenance software locates.
Restore
This button is similar to Read, except that when selecting Restore you must not
select a target file, and the system will read the target file from the directory where the
test and maintenance software locates.
This chapter provides the system warning messages and recommended corrective
actions, which should be taken in time once any error occurs.
When an error or failure occurs, relevant alarm message will be displayed, and the
system will take corresponding actions.
The alarm message will be displayed in the alarm message area of the software
screen, and then recorded in the system log automatically.
The logs will record the time, level, code and detailed information of each warning to
help user record and search errors.
In case of a warning message, check its error code on the Logs screen of the
operating software, and find recommended actions in the Solution field.
10 Troubleshooting 10-1
Figure10-1 Warning Message Area
Severity: Warning
10-2 10 Troubleshooting
instruction and rerun the tests.
Severity: Pausing
Severity: Forbidding
10 Troubleshooting 10-3
14 Errors to forbid ISE When the ISE module is configured but cannot
work normally, the system will forbid running
ISE analytes.
15 Errors to forbid sample When the sample bar code reader is
bar code reader configured but cannot work normally, the
system will forbid scanning samples.
16 Errors to forbid reagent When the reagent bar code reader is
bar code reader configured but cannot work normally, the
system will forbid scanning reagents.
17 Errors to forbid fluidic The system will prohibit the washing function.
system
WARNING
When troubleshooting the analyzer, first find out whether it is necessary
to switch off the Main Power or Analyzing Unit Power.
BIOHAZARD
Wear gloves and lab coat and, if necessary, goggles.
10-4 10 Troubleshooting
Error Level Error Probable Causes Corrective Actions
Code Message
C0005 0 Rearrange hard disk.
Insufficient Remaining disk space is
Delete curves and
disk space less than 1G
other unuseful files
C0006 19 Rearrange hard disk.
Out of disk Remaining disk space is
Delete curves and
space less than 200M
other unuseful files
C0007 19 CPU
CPU is below Celeron
performance Replace PC or CPU
733
low
C0008 0 Check printer
Printer is not powered connection. Check if
Printer
on. Cable is not printer is powered
cannot be
connected. No driver is on, driver and default
connected
installed printer has been
installed
C0009 0 Check for paper jam.
Printer Paper jam. No paper. Check if printer is
failure No ink busy, and print tasks
are too many
C0010 0 Help No help document. Help Check if help
document document is damaged. document exists or is
error No memory space damaged
C0011 0 No sound card is
Sound card installed. Sound card Reinstall sound card
failure failure. Incorrect sound or sound card driver
card driver
10 Troubleshooting 10-5
Error Level Error Probable Causes Corrective Actions
Code Message
powered on. Start
initialization again.
Restart PC and
analyzing unit
10.2.1.3 Calculation
Error Level Error Probable Causes Corrective Actions
Code Message
C0301 0 (%s Reagent blank
Replace reagent.
test)Calibratio absorbance is too
Replace calibrator.
n sensitivity high. Calibrator is
Recalibrate
error degenerated
C0302 0 (%s
test)Coefficien Calibrator goes Replace reagent.
t difference wrong. Reagent goes Replace calibrator.
limit is out of wrong Recalibrate
range
C0303 0 (%s
test)Correlatio
Calibrator goes Replace reagent.
n
wrong. Reagent goes Replace calibrator.
coefficient(R2
wrong Recalibrate
) is out of
range
C0304 0 (%s
Calibrator goes Replace reagent.
test)Reaction
wrong. Reagent goes Replace calibrator.
curve SD is
wrong Recalibrate
out of range
C0305 0 (%s Results cannot be
Replace reagent.
test)Calibratio calculated by
Replace calibrator.
n parameters specified rule. Results
Recalibrate or
cannot be are abnormal.
recalculate calibration
calculated by Calibration is not
parameters
given method convergent
C0306 0 (%s
Reagent blank
test)Absorban Replace reagent.
absorbance is too
ce of 0 Replace calibrator.
high. Calibrator is
calibrator is Recalibrate
degenerated
out of range
C0307 0 (%s
Calibration replicates
test)Calibratio
are unfinished.
n data is Fill reagent and
Reagent is
incomplete. calibrator. Recalibrate
insufficient. Calibrator
Cannot
is insufficient
calculate
C0308 0 (%s test and
%d Key points are lost
sample)Resp during response Rerun
onse calculate calculation
error
C0309 0 (%s test and Abnormal sample
%d (hemolysis, etc).
Run diluted sample, or
sample)Resp Calibrator
recalibrate
onse is out of concentration is too
range low
10-6 10 Troubleshooting
Error Level Error Probable Causes Corrective Actions
Code Message
C0310 0 If problem occurs
Communication
Received data frequently, reconnect
between analyzing
check sum serial cable. If problem
unit and operation
error remains, contact the
unit is interfered
developer
C0311 0 Control is
(%s
degenerated. Rerun. Replace control
test)Real-time
Reagent goes wrong. or reagent and rerun.
QC 12s
Light intensity is Replace light source
warning
abnormal
C0312 0 Control is
(%s
degenerated. Rerun. Replace control
test)Real-time
Reagent goes wrong. or reagent and rerun.
QC of 13s is
Light intensity is Replace light source
out of control
abnormal
C0313 0 Control is
(%s
degenerated. Rerun. Replace control
test)Real-time
Reagent goes wrong. or reagent and rerun.
QC of 22s is
Light intensity is Replace light source
out of control
abnormal
C0314 0 Control is
(%s
degenerated. Rerun. Replace control
test)Real-time
Reagent goes wrong. or reagent and rerun.
QC R4s is out
Light intensity is Replace light source
of control
abnormal
C0315 0 Control is
(%s
degenerated. Rerun. Replace control
test)Real-time
Reagent goes wrong. or reagent and rerun.
QC 41s is out
Light intensity is Replace light source
of control
abnormal
C0316 0 Control is
(%s
degenerated. Rerun. Replace control
test)Real-time
Reagent goes wrong. or reagent and rerun.
QC 10x is out
Light intensity is Replace light source
of control
abnormal
C0317 0 (%s
Calibrator goes
test)Calibratio Replace reagent or
wrong. Reagent goes
n repeatability calibrator. Recalibrate
wrong
is out of range
C0318 0 (%s
Incorrect reagent
test)Multi-poin
dispensing volume. Retest un-monotone
t or nonlinear
Incorrect calibrator points. Recalibrate
calibration is
dispensing volume.
not monotone
C0319 0 (%s)Result Abnormal test result. Rerun participated
cannot be Error occurs like 0 tests. Check and reset
calculated dividend calculation formula
C0320 0 (%d
sample/%s Response error.
test)Concentr Calibration formula Rerun or recalculate
ation cannot error
be calculated
C0321 0 Absorbance is Rerun, or rerun after
Sample goes wrong.
out of linearity replacing reagent or
Reagent goes wrong
range sample
10 Troubleshooting 10-7
Error Level Error Probable Causes Corrective Actions
Code Message
C0322 0 (%s test and Antigen excess. Too
%d much sample.
Dilute and rerun
sample)Prozo Sample concentration
ne check error is too high.
C0323 0 (%s test and
%d
Reagent is stored too Rerun after replacing
sample)R1
long or expired reagent
blank exceeds
limit
C0324 0 (%s test and
%d
sample)No
Rerun, or rerun after
linear interval Unsteady reagent.
replacing reagent or
in Kinetic Sample goes wrong
sample
analysis.
Cannot
calculate
10-8 10 Troubleshooting
Error Level Error Probable Causes Corrective Actions
Code Message
C0504 0 Reset barcode format,
%s barcode Barcode is in wrong
or reprint or rescan
error format
barcode
10 Troubleshooting 10-9
Error Level Error Probable Causes Corrective Actions
Code Message
C0608 0 If problem occurs
accidentally, send or
receive again. If
Wrong Communication
problem occurs
process ID failure
frequently, consult
developer of LIS or
equipment
C0609 0 If problem occurs
accidentally, send or
receive again. If
Wrong version Communication
problem occurs
No. failure
frequently, consult
developer of LIS or
equipment
C0610 0 If problem occurs
accidentally, send or
Unknown receive again. If
Communication
keyword problem occurs
failure
identity frequently, consult
developer of LIS or
equipment
C0611 0 If problem occurs
accidentally, send or
Keyword receive again. If
Communication
identity problem occurs
failure
already exists frequently, consult
developer of LIS or
equipment
C0612 0 If problem occurs
accidentally, send or
receive again. If
Unknown Communication
problem occurs
error failure
frequently, consult
developer of LIS or
equipment
C0613 0 Neglect. If problem
Your query
occurs frequently,
does not exist LIS failure
contact developer of
on LIS
LIS or equipment
C0614 13 Send and receive again
LIS host is
after a moment, or
busy. Cannot LIS failure
reconnect to LIS. Reset
respond
LIS
C0615 0 Check network
connection. If problem
LIS does not start.
LIS response occurs continuously for
Communication
is timed out 3 times, contact
failure
developer of LIS or
equipment
C0616 0 Check if LIS host works
normally. Reset LIS
host. If problem occurs
Application LIS host database
continuously for 3
record locked error
times, contact the LIS
manufacture or
equipment developer
10-10 10 Troubleshooting
10.2.1.7 Others
Error Level Error Probable Causes Corrective Actions
Code Message
C0701 2 All reagents in this
kind of bottles do not
Fill reagent of this test,
%s test has reach minimum limit.
or replace it with new
no enough %s All reagents of this
reagent
kind cannot be
detected
C0702 11 Check if equipment is
Analyzing unit is busy
Test period powered on. Restore
and cannot return
timed out. failure. Communicate
result, serial
Cannot with control software.
communication error,
continue Restart analyzing unit
or power failure
and operation unit
C0703 12 Retest dark current on
Utilities-->Daily Maint.
Dark current Excessive circuit
page. Adjust
is too high noise
photoelectric gain.
Contact your developer
C0704 12 Reset the main unit on
Analyzing unit Parameter
Utilities-->Daily Maint.
reset failed downloading failed
page
C0705 11 Restore failure on
Utilities-->Daily Maint.
Analyzing unit
Sensor failure. page. Restart analyzing
failure cannot
Motor/Belt failure unit and operation unit.
recover
Contact equipment
developer
C0706 0 No floppy disk or U
Storage disk is inserted. Check if U disk or
device error. Insufficient disk floppy disk is inserted or
Cannot export space. Floppy disk or full. Check if storage
data U disk is locked or device is damaged
damaged
C0707 0 No floppy disk or U
disk is inserted. File
Storage Check if U disk or
does not exist. File
device error. floppy disk is inserted or
error. File is
Cannot import full. Check if storage
damaged. Floppy
data device is damaged
disk or U disk is
locked or damaged
C0708 0 Insufficient
Insufficient acid wash Add acid wash solution
acid wash
solution on reagent on specified position of
solution on
disk reagent disk
reagent disk
C0709 0 Insufficient
Insufficient alkaline Add alkaline wash
alkaline wash
wash solution on solution on specified
solution on
reagent disk position of reagent disk
reagent disk
C0710 2 Insufficient
Add distilled water on
distilled water Insufficient distilled
specified position of
on reagent water on reagent disk
reagent disk
disk
10 Troubleshooting 10-11
Error Level Error Probable Causes Corrective Actions
Code Message
C0711 0 Insufficient
Insufficient acid wash Add acid wash solution
acid wash
solution on sample on specified position of
solution on
disk sample disk
sample disk
C0712 0 Insufficient
Insufficient alkaline Add alkaline wash
alkaline wash
wash solution on solution on specified
solution on
sample disk position of sample disk
sample disk
C0713 3 Insufficient
Add distilled water on
distilled water Insufficient distilled
specified position of
on sample water on sample disk
sample disk
disk
C0714 12 Check if lamp is turned
on, and check the
cuvettes on
Utilities-->Daily Maint.
Page, then replace the
cuvettes marked by
Lamp aged. Lamp is colors. Check the lamp
Light intensity not turned on. Lamp on If failure remains,
is weak is loose. All cuvettes replace the lamp
are dirty Utilities-->Daily Maint.
Page. If light intensity is
not strong enough,
replace the lamp. If
failure still remains,
contact equipment
developer
C0715 0 Check if lamp is turned
on, and check the
cuvettes on
Utilities-->Daily Maint.
Page, then replace the
Cuvette is dirty. The cuvettes marked by
amount of water to colors. Check the lamp
Blank of
measure the six-th on If failure remains,
cuvette %s
phase water blank is replace the lamp
exceeds limit
not enough. Light Utilities-->Daily Maint.
intensity is too weak Page. If light intensity is
not strong enough,
replace the lamp. If
failure still remains,
contact equipment
developer
C0716 11 Received data is too
Received data Contact equipment
much and exceeds
overflow developer
buffer capacity
10-12 10 Troubleshooting
Error Level Error Probable Causes Corrective Actions
Code Message
C0718 14 Calibrate the ISE
ISE test results are module when the
not received in given system is paused or
ISE test is
time; ISE module is idle. If problem occurs
timed out
not connected continuously for 3 times
correctly or other error occurs,
contact the developer
C0719 12 Check if lamp is
installed correctly and
Lamp is not turned
turned on. If failure
on; bulb is damaged;
remains after replacing
no lamp is installed;
Lamp is not the lamp, contact the
lamp is loose; foreign
turned on develop. Check if
matter exist in three
foreign matters exist in
continuous cuvettes
continuous three
to obstruct light path
cuvettes. Replace the
cuvettes if necessary
C0720 12 No cuvettes are Check if all positions of
No reaction installed in four reaction disk are
cuvettes, or continuous positions; occupied. If yes, ask
lamp intensity photoelectric gain our service personnel to
is too strong exceeds the adjust the photoelectric
measurement range gain
C0721 1 Check for failed cuvette
and replace it. If the
Foreign matters exist
Clots are error remains, check if
to obstruct light path
found in the lamp is installed
so that the measured
No.%s tightly. If the error still
value is less than
cuvette remains for all new
1000
cuvettes, contact our
service personnel
10 Troubleshooting 10-13
Error Level Error Probable Causes Corrective Actions
Code Message
A0003 11 Wait for 30-60s and
Downloading retry. If system does not
Main unit is
parameters to respond for long time,
busy
subunits. Cannot restore failure. If this
downloading
respond to other problem occurs
parameters
instruction frequently, contact the
developer
A0004 12 Restore failure on
Utilities-->Daily Maint.
Self-test error Self-test error page. If this message
appears for 3 times,
contact the developer
A0005 11 Switch off analyzing unit
power and switch on
again. Restore failure
Invalid
Instruction execute on Utilities-->Daily
instruction in
error Maint. page. If this
current status
message appears for 3
times, contact the
developer
A0006 11 Switch off analyzing unit
power and switch on
System is
Executing other again. Restore failure
busy. Cannot
instruction. Cannot on Utilities-->Daily
respond to
respond to current Maint. page. If this
other
one message appears for 3
operation
times, contact the
developer
A0007 11 Switch off analyzing unit
power and switch on
again. Restore failure
Instruction Instruction execute on Utilities-->Daily
execute error error Maint. page. If this
message appears for 3
times, contact the
developer
A0008 11 Switch off analyzing unit
power and switch on
again. Restore failure
E2PROM read/write on Utilities-->Daily
Memory error
error Maint. page. If this
message appears for 3
times, contact the
developer
A0009 0 Received data in Rerun. If problem
Photoelectric
single period is less occurs frequently,
data is lost
than 90 contact the developer
A0010 11 If optic measurement
assembly goes wrong,
replace AD assembly. If
AD collection board
Photoelectric AD value is too low works normally,
output is (below 10) or too high remove optic
abnormal (over 65500) measurement
assembly, and check if
preamplification board
and optical path are
normal
10-14 10 Troubleshooting
Error Level Error Probable Causes Corrective Actions
Code Message
A0011 11 Photoelectric data
Photoelectric Restart analyzing unit
buffer is full. Cannot
data overflow and operation unit
process new data
A0012 11 Photoelectric circuit
Photoelectric
does not return result Restart analyzing unit
collection is
via FIFO in specified and operation unit
timed out
time
A0013 11
Restart analyzing unit
Downloading Incompatible or and replace with new
failed wrong version version. If failed again,
contact the developer
10 Troubleshooting 10-15
Error Level Error Probable Causes Corrective Actions
Code Message
A0102 11 Reset analyzing unit
power and restart
operating software.
Instruction Instruction parameter
Then retry this
parameter does not comply with
instruction. If this
error protocol
message appears for 3
times, contact the
developer
A0103 6 Switch off analyzing unit
power and switch on
again. Download
Unit is busy. Unit parameters and restore
No execute
does not reset failure on
condition
mechanically Utilities-->Daily Maint.
page. If this message
appears for 3 times,
contact the developer
A0104 6 Wrong Switch off analyzing unit
sensor power and switch on
status when again. Restore failure
sample Vertical position on Utilities-->Daily
probe sensor failure Maint. page. If this
moves message appears for 3
vertically(oth times, contact the
er position) developer
A0105 11 Wrong
Switch off analyzing unit
sensor
power and switch on
status when
again. Restore failure
sample
Vertical position on Utilities-->Daily
probe
sensor failure Maint. page. If this
moves
message appears for 3
vertically(in
times, contact the
reaction
developer
disk)
A0106 6 Sample
probe
cannot find
Vertical position
home Restore failure. If failed
sensor failure.
position for 3 times, contact the
Obstruction exists in
when developer
vertical direction
moving
vertically(oth
er position)
A0107 6 Sample
probe
cannot find
home Vertical position
Restore failure. If failed
position sensor failure.
for 3 times, contact the
when Obstruction exists in
developer
moving vertical direction
vertically(in
reaction
disk)
10-16 10 Troubleshooting
Error Level Error Probable Causes Corrective Actions
Code Message
A0108 18 Sample
probe
If auto reset fails,
bumps when Wrong position.
restore failure. Remove
moving Obstruction exists
obstruction and reset
vertically(oth
er position)
A0109 18 Sample
probe
If auto reset fails,
bumps when Wrong position.
restore failure. Remove
moving Obstruction exists
obstruction and reset
vertically(IS
E unit)
A0110 18 Sample
probe
bumps when If auto reset fails,
Wrong position.
moving restore failure. Remove
Obstruction exists
vertically(in obstruction and reset
reaction
disk)
A0111 6 Lowering
down at Sample probe is not
If auto reset fails,
current in vertical home
restore failure. If failed
position is position. Current
for 3 times, contact the
not position is not proper
developer
allowed(oth for lowering down
er position)
A0112 11 Lowering
down at
Sample probe is not
current If auto reset fails,
in vertical home
position is restore failure. If failed
position. Current
not for 3 times, contact the
position is not proper
allowed(in developer
for lowering down
reaction
disk)
A0113 6 Wrong
Switch off analyzing unit
sensor
power and switch on
status when
again. Restore failure
sample
Rotational position on Utilities-->Daily
probe
sensor failure Maint. page. If this
moves
message appears for 3
horizontally(
times, contact the
other
developer
position)
A0114 14 Wrong Switch off analyzing unit
sensor power and switch on
status when again. Restore failure
sample Rotational position on Utilities-->Daily
probe sensor failure Maint. page. If this
moves message appears for 3
horizontally( times, contact the
ISE unit) developer
10 Troubleshooting 10-17
Error Level Error Probable Causes Corrective Actions
Code Message
A0115 11 Wrong
Switch off analyzing unit
sensor
power and switch on
status when
again. Restore failure
sample
Rotational position on Utilities-->Daily
probe
sensor failure Maint. page. If this
moves
message appears for 3
horizontally(i
times, contact the
n reaction
developer
disk)
A0116 6 Sample
probe
cannot find
home Rotational position
Restore failure. If failed
position sensor failure.
for 3 times, contact the
when Obstruction exists in
developer
moving horizontal direction
horizontally(
other
position)
A0117 11 Sample
probe
cannot find
home Rotational position
Restore failure. If failed
position sensor failure.
for 3 times, contact the
when Obstruction exists in
developer
moving horizontal direction
horizontally(i
n reaction
disk)
A0118 18 Sample
probe
collides Sample probe is
If auto reset fails,
when obstructed or falls
restore failure. Check
moving when moving
motor and belt
horizontally( horizontally.
other
position)
A0119 11 Sample
probe
collides Sample probe is
If auto reset fails,
when obstructed or falls
restore failure. Check
moving when moving
motor and belt
horizontally(i horizontally.
n reaction
disk)
A0120 6 Sample probe is not
Rotating at
in horizontal home If auto reset fails,
current
position. Current restore failure. If failed
height is not
height is not proper for 3 times, contact the
allowed(oth
for rotation(highest developer
er position)
position)
A0121 11 Rotating at Sample probe is not
current in horizontal home If auto reset fails,
height is not position. Current restore failure. If failed
allowed(in height is not proper for 3 times, contact the
reaction for rotation(highest developer
disk) position)
10-18 10 Troubleshooting
Error Level Error Probable Causes Corrective Actions
Code Message
A0122 6 If auto reset fails,
Syringe
restore failure. If failed
sensor is in Syringe sensor error
for 3 times, contact the
wrong status
developer
A0123 6 Check if sample syringe
reaches maximum limit
Sample syringe
Syringe and cannot restore.
reaches maximum
cannot find Restore failure, or reset
stroke. Cannot
home after pushing syringe to
restore or dispense
position home position. If failed
sample
for 3 times, contact the
developer
A0124 3 Clog Wash sample probe.
Sample probe is
detection Remove probe and
clogged
error clear up foreign matters
A0125 11 Sample
probe does
not detect
No deionized water Add deionized water
wash
solution
level
A0126 1 Sample
probe does
not detect No R1 dispensed. Check if reagent is
liquid level Insufficient R1 sufficient. Rerun
on reaction
disk
A0127 3 Sample
probe does
No sample tube.
not detect Check if sample is
Sample is already
liquid level sufficient. Rerun
depleted
on sample
disk
A0128 1 Insufficient
sample Insufficient sample Check sample volume
dispensing aspiration volume and rerun
volume
A0129 1 Sample
Sample syringe Restore failure. If failed
syringe
aspirates full for 3 times, contact the
aspirates
abnormally developer
too much
A0130 1 Sample
Sample syringe Restore failure. If failed
syringe
dispenses empty for 3 times, contact the
dispenses
abnormally developer
too much
A0131 1 Sample
Add samples or replace
probe does Insufficient sample.
with standard sample
not aspirate Wrong tube type
tube
sample
A0132 0 Re-centrifugate sample,
Clogs in
Clots in sample or remove clots
sample
manually
10 Troubleshooting 10-19
Error Level Error Probable Causes Corrective Actions
Code Message
A0133 11 Switch off analyzing unit
power and switch on
Execution again. Restore failure
result is not Instruction execution on Utilities-->Daily
received in is timed out Maint. page. If this
given time message appears for 3
times, contact the
developer
A0134 11 Switch off analyzing unit
power and switch on
No again. Restore failure
response, or Instruction execute on Utilities-->Daily
response error Maint. page. If this
error message appears for 3
times, contact the
developer
10-20 10 Troubleshooting
Error Level Error Probable Causes Corrective Actions
Code Message
A0206 6 Restore failure on
Utilities-->Daily Maint.
Wrong sensor
Sensor failure page. If this message
status
appears for 3 times,
contact the developer
A0207 15 Sample barcode
Bar code
reader is not installed. Reboot the analyzer. If
reader does
Barcode reader is not problem remains,
not work
connected to PCB contact the developer
normally
properly
A0208 0 Check if barcode label
Barcode digit error. is dirty, skewed, or
Bar code
Data format error. No placed correctly.
error
end mark Rescan or scan after
reprinting
A0209 15 Rescan after restoring
Bar code
Scanning too many or failure. If this message
sending
too fast appears for 3 times,
buffer is full
contact the developer
A0210 11 Switch off analyzing
unit power and switch
Execution on again. Restore
result is not Instruction execution failure on
received in is timed out Utilities-->Daily Maint.
given time page. If this message
appears for 3 times,
contact the developer
A0211 11 Switch off analyzing
unit power and switch
on again. Restore
No response,
Instruction execute failure on
or response
error Utilities-->Daily Maint.
error
page. If this message
appears for 3 times,
contact the developer
10 Troubleshooting 10-21
Error Level Error Probable Causes Corrective Actions
Code Message
A0302 11 Reset analyzing unit
power and restart
operating software.
Instruction Instruction parameter
Then retry this
parameter does not comply with
instruction. If this
error protocol
message appears for 3
times, contact the
developer
A0303 4 Switch off analyzing unit
power and switch on
again. Download
Unit is busy and does parameters and restore
No execute
not reset failure on
condition
mechanically Utilities-->Daily Maint.
page. If this message
appears for 3 times,
contact the developer
A0304 4 Switch off analyzing unit
Wrong sensor power and switch on
status when again. Restore failure
R1 probe Vertical position on Utilities-->Daily
moves sensor failure Maint. page. If this
vertically(othe message appears for 3
r position) times, contact the
developer
A0305 11 Switch off analyzing unit
Wrong sensor power and switch on
status when again. Restore failure
R1 probe Vertical position on Utilities-->Daily
moves sensor failure Maint. page. If this
vertically(in message appears for 3
reaction disk) times, contact the
developer
A0306 4 R1 probe
cannot find Vertical position
Restore failure. If failed
home position sensor failure.
for 3 times, contact the
when moving Obstruction exists in
developer
vertically(othe vertical direction
r position)
A0307 11 R1 probe
cannot find Vertical position
Restore failure. If failed
home position sensor failure.
for 3 times, contact the
when moving Obstruction exists in
developer
vertically(in vertical direction
reaction disk)
A0308 18 R1 probe
bumps when If auto reset fails,
Wrong position.
moving restore failure. Remove
Obstruction exists
vertically(othe obstruction and reset
r position)
A0309 18 R1 probe
bumps when If auto reset fails,
Wrong position.
moving restore failure. Remove
Obstruction exists
vertically(in obstruction and reset
reaction disk)
10-22 10 Troubleshooting
Error Level Error Probable Causes Corrective Actions
Code Message
A0310 4 Lowering
R1 probe is not in
down at If auto reset fails,
vertical home
current restore failure. If failed
position. Current
position is not for 3 times, contact the
position is not proper
allowed(other developer
for lowering down
position)
A0311 11 Lowering
R1 probe is not in
down at If auto reset fails,
vertical home
current restore failure. If failed
position. Current
position is not for 3 times, contact the
position is not proper
allowed(in developer
for lowering down
reaction disk)
A0312 4 Switch off analyzing unit
Wrong sensor power and switch on
status when again. Restore failure
R1 probe Rotational position on Utilities-->Daily
moves sensor failure Maint. page. If this
horizontally(ot message appears for 3
her position) times, contact the
developer
A0313 11 Switch off analyzing unit
Wrong sensor
power and switch on
status when
again. Restore failure
R1 probe Rotational position
on Utilities-->Daily
moves sensor failure
Maint. If this message
horizontally(in
appears for 3 times,
reaction disk)
contact the developer
A0314 4 R1 probe
cannot find Rotational position
Restore failure. If failed
home position sensor failure.
for 3 times, contact the
when moving Obstruction exists in
developer
horizontally(ot horizontal direction
her position)
A0315 11 R1 probe
cannot find Rotational position
Restore failure. If failed
home position sensor failure.
for 3 times, contact the
when moving Obstruction exists in
developer
horizontally(in horizontal direction
reaction disk)
A0316 18 R1 probe
R1 probe is
collides when If auto reset fails,
obstructed or falls
moving restore failure. Check
when moving
horizontally(ot motor and belt
horizontally
her position)
A0317 11 R1 probe
R1 probe is
collides when If auto reset fails,
obstructed or falls
moving restore failure. Check
when moving
horizontally(in motor and belt
horizontally
reaction disk)
A0318 4 R1 probe is not in
horizontal home If auto reset fails,
Rotating is not
position. Current restore failure. If failed
allowed(other
height is not proper for 3 times, contact the
position)
for rotation(highest developer
position)
10 Troubleshooting 10-23
Error Level Error Probable Causes Corrective Actions
Code Message
A0319 11 R1 probe is not in
horizontal home If auto reset fails,
Rotating is not
position. Current restore failure. If failed
allowed(in
height is not proper for 3 times, contact the
reaction disk)
for rotation(highest developer
position)
A0320 4 If auto reset fails,
Syringe
restore failure. If failed
sensor is in Syringe sensor error
for 3 times, contact the
wrong status
developer
A0321 4 Check if R1 syringe
reaches maximum limit
and cannot restore.
R1 syringe reaches
Syringe Restore failure, or reset
maximum stroke.
cannot find after pushing syringe to
Cannot restore or
home position home position. If this
dispense reagent
problem occurs for 3
times, contact the
developer
A0322 11 R1 probe
does not
No deionized water Add deionized water
detect wash
solution level
A0323 1 R1 probe No R1 or sample or
does not insufficient R1 in
Check if reagent is
detect liquid reaction cuvette when
sufficient. Rerun
level on system dispenses R1
reaction disk or R3
A0324 2 R1 probe
does not No reagent on first
Check if reagent is
detect liquid reagent position.
sufficient. Rerun
level on Reagent is depleted
reagent disk
A0325 1 Insufficient R1
Insufficient R1 Check reagent volume
dispensing
aspiration volume and rerun
volume
A0326 1 R1 syringe Restore failure. If failed
R1 syringe aspirates
aspirates too for 3 times, contact the
full abnormally
much developer
A0327 1 R1 syringe Restore failure. If failed
R1 syringe dispenses
dispenses too for 3 times, contact the
empty abnormally
much developer
A0328 11 Switch off analyzing unit
power and switch on
Execution again. Restore failure
result is not Instruction execution on Utilities-->Daily
received in is timed out Maint. page. If this
given time message appears for 3
times, contact the
developer
A0329 11 Switch off analyzing unit
power and switch on
again. Restore failure
No response,
Instruction execute on Utilities-->Daily
or response
error Maint. page. If this
error
message appears for 3
times, contact the
developer
10-24 10 Troubleshooting
10.2.2.5 R2 Probe Unit
Error Level Error Probable Causes Corrective Actions
Code Message
A0401 5 Reset analyzing unit
power and restart
Instruction format operating software.
Instruction error. Invalid Then retry this
format error information is instruction. If this
included message appears for 3
times, contact the
developer
A0402 11 Reset analyzing unit
power and restart
operating software.
Instruction Instruction parameter
Then retry this
parameter does not comply with
instruction. If this
error protocol
message appears for 3
times, contact the
developer
A0403 5 Switch off analyzing unit
power and switch on
again. Download
Unit is busy and does parameters and restore
No execute
not reset failure on
condition
mechanically Utilities-->Daily Maint.
page. If this message
appears for 3 times,
contact the developer
A0404 5 Switch off analyzing unit
Wrong
power and switch on
sensor
again. Restore failure
status when
Vertical position on Utilities-->Daily
R2 probe
sensor failure Maint. page. If this
moves
message appears for 3
vertically(ot
times, contact the
her position)
developer
A0405 11 Wrong Switch off analyzing unit
sensor power and switch on
status when again. Restore failure
R2 probe Vertical position on Utilities-->Daily
moves sensor failure Maint. page. If this
vertically(in message appears for 3
reaction times, contact the
disk) developer
A0406 5 R2 probe
cannot find
home Vertical position
Restore failure. If failed
position sensor failure.
for 3 times, contact the
when Obstruction exists in
developer
moving vertical direction
vertically(ot
her position)
10 Troubleshooting 10-25
Error Level Error Probable Causes Corrective Actions
Code Message
A0407 11 R2 probe
cannot find
home
Vertical position
position Restore failure. If failed
sensor failure.
when for 3 times, contact the
Obstruction exists in
moving developer
vertical direction
vertically(in
reaction
disk)
A0408 18 R2 probe
bumps
If auto reset fails,
when Wrong position.
restore failure. Remove
moving Obstruction exists
obstruction and reset
vertically(ot
her position)
A0409 18 R2 probe
bumps
when If auto reset fails,
Wrong position.
moving restore failure. Remove
Obstruction exists
vertically(in obstruction and reset
reaction
disk)
A0410 5 Lowering
down at R2 probe is not in
If auto reset fails,
current vertical home
restore failure. If failed
position is position. Current
for 3 times, contact the
not position is not proper
developer
allowed(oth for lowering down
er position)
A0411 11 Lowering
down at
R2 probe is not in
current If auto reset fails,
vertical home
position is restore failure. If failed
position. Current
not for 3 times, contact the
position is not proper
allowed(in developer
for lowering down
reaction
disk)
A0412 5 Wrong Switch off analyzing unit
sensor power and switch on
status when again. Restore failure
R2 probe Rotational position on Utilities-->Daily
moves sensor failure Maint. page. If this
horizontally( message appears for 3
other times, contact the
position) developer
A0413 11 Wrong Switch off analyzing unit
sensor power and switch on
status when again. Restore failure
R2 probe Rotational position on Utilities-->Daily
moves sensor failure Maint. page. If this
horizontally( message appears for 3
in reaction times, contact the
disk) developer
10-26 10 Troubleshooting
Error Level Error Probable Causes Corrective Actions
Code Message
A0414 5 R2 probe
cannot find
home
Rotational position
position Restore failure. If failed
sensor failure.
when for 3 times, contact the
Obstruction exists in
moving developer
horizontal direction
horizontally(
other
position)
A0415 11 R2 probe
cannot find
home
Rotational position
position Restore failure. If failed
sensor failure.
when for 3 times, contact the
Obstruction exists in
moving developer
horizontal direction
horizontally(
in reaction
disk)
A0416 18 R2 probe
collides
R2 probe is
when If auto reset fails,
obstructed or falls
moving restore failure. Check
when moving
horizontally( motor and belt
horizontally
other
position)
A0417 11 R2 probe
collides
R2 probe is
when If auto reset fails,
obstructed or falls
moving restore failure. Check
when moving
horizontally( motor and belt
horizontally
in reaction
disk)
A0418 5 R2 probe is not in
Rotating is horizontal home If auto reset fails,
not position. Current restore failure. If failed
allowed(oth height is not proper for 3 times, contact the
er position) for rotation(highest developer
position)
A0419 11 R2 probe is not in
Rotating is
horizontal home If auto reset fails,
not
position. Current restore failure. If failed
allowed(in
height is not proper for 3 times, contact the
reaction
for rotation(highest developer
disk)
position)
A0420 5 Syringe If auto reset fails,
sensor is in restore failure. If failed
Syringe sensor error
wrong for 3 times, contact the
status developer
A0421 5 Check if R2 syringe
reaches maximum limit
and cannot restore.
Syringe R2 syringe reaches
Restore failure, or reset
cannot find maximum stroke.
after pushing syringe to
home Cannot restore or
home position. If this
position dispense reagent
problem occurs for 3
times, contact the
developer
10 Troubleshooting 10-27
Error Level Error Probable Causes Corrective Actions
Code Message
A0422 11 R2 probe
does not
detect wash No deionized water Add deionized water
solution
level
A0423 1 R2 probe
does not
No R1 or sample or
detect liquid Check if reagent is
insufficient R1 in
level on sufficient. Rerun
cuvette
reaction
disk
A0424 2 R2 probe
No reagent on
does not
second reagent Check if reagent is
detect liquid
position. Reagent is sufficient. Rerun
level on
depleted
reagent disk
A0425 1 Insufficient
R2 Insufficient R2 Check reagent volume
dispensing aspiration volume and rerun
volume
A0426 1 R2 syringe Restore failure. If failed
R2 syringe aspirates
aspirates for 3 times, contact the
full abnormally
too much developer
A0427 1 R2 syringe Restore failure. If failed
R2 syringe dispenses
dispenses for 3 times, contact the
empty abnormally
too much developer
A0428 11 Switch off analyzing unit
power and switch on
Execution again. Restore failure
result is not Instruction execution on Utilities-->Daily
received in is timed out Maint. page. If this
given time message appears for 3
times, contact the
developer
A0429 11 Switch off analyzing unit
power and switch on
No again. Restore failure
response, Instruction execute on Utilities-->Daily
or response error Maint. page. If this
error message appears for 3
times, contact the
developer
10-28 10 Troubleshooting
Error Level Error Probable Causes Corrective Actions
Code Message
A0502 11 Reset analyzing unit
power and restart
operating software.
Instruction Instruction parameter
Then retry this
parameter does not comply with
instruction. If this
error protocol
message appears for 3
times, contact the
developer
A0503 7 Switch off analyzing unit
power and switch on
again. Download
Unit is busy and does parameters and restore
No execute
not reset failure on
condition
mechanically Utilities-->Daily Maint.
page. If this message
appears for 3 times,
contact the developer
A0504 7 Restore failure. If this
Home
Cannot find home message appears for 3
position is
position times, contact the
not found
developer
A0505 7 Restore failure. If this
Step message appears for 3
Belt failure
missed times, contact the
developer
A0506 7 Restore failure. If this
Wrong
message appears for 3
sensor Sensor failure
times, contact the
status
developer
A0507 16 Reagent barcode
Bar code
reader is not installed. Reboot the analyzer. If
reader does
Barcode reader is not problem remains,
not work
connected to PCB contact the developer
normally
properly
A0508 0 Check if barcode label
Barcode digit error. is dirty, skewed, or
Bar code
Data format error. No placed correctly.
error
end mark Rescan or scan after
reprinting
A0509 16 Rescan after resetting
Bar code mechanically. If this
Scanning too many or
sending message appears for 3
too fast
buffer is full times, contact the
developer
A05010 11 Switch off analyzing unit
power and switch on
Execution again. Restore failure
result is not Instruction execution on Utilities-->Daily
received in is timed out Maint. page. If this
given time message appears for 3
times, contact the
developer
10 Troubleshooting 10-29
Error Level Error Probable Causes Corrective Actions
Code Message
A05011 11 Switch off analyzing unit
power and switch on
No again. Restore failure
response, Instruction execute on Utilities-->Daily
or response error Maint. page. If this
error message appears for 3
times, contact the
developer
10-30 10 Troubleshooting
A0609 12 Switch off analyzing
unit power and switch
Photoelectric Photoelectric buffer
on again. If this
buffer is overflow, or FIFO
message appears for 3
abnormal overflow
times, contact the
developer
A0610 1 After testing, switch off
analyzing unit power
Photoelectric
Photoelectric and switch on again. If
collection and
data error this message appears
conversion failed
for 3 times, contact the
developer
A0611 11 Switch off analyzing
unit power and switch
Execution on again. Restore
result is not Instruction execution failure on
received in is timed out Utilities-->Daily Maint.
given time page. If this message
appears for 3 times,
contact the developer
A0612 11 Switch off analyzing
unit power and switch
No on again. Restore
response, or Instruction execute failure on
response error Utilities-->Daily Maint.
error page. If this message
appears for 3 times,
contact the developer
10 Troubleshooting 10-31
Error Level Error Message Probable Causes Corrective Actions
Code
A0703 9 Switch off analyzing
unit power and
switch on again.
Download
Unit is busy and parameters and
No execute
does not reset restore failure on
condition
mechanically Utilities-->Daily
Maint. page. If this
message appears
for 3 times, contact
the developer
A0704 9 Switch off analyzing
unit power and
Wrong sensor
switch on again.
status when
Restore failure on
reagent mixer Vertical position
Utilities-->Daily
moves sensor failure
Maint. page. If this
vertically(other
message appears
position)
for 3 times, contact
the developer
A0705 11 Switch off analyzing
unit power and
Wrong sensor
switch on again.
status when
Restore failure on
reagent mixer Vertical position
Utilities-->Daily
moves sensor failure
Maint. page. If this
vertically(in
message appears
reaction disk)
for 3 times, contact
the developer
A0706 9 Reagent mixer
cannot find home Vertical position Restore failure. If
position when sensor failure. failed for 3 times,
moving Obstruction exists contact the
vertically(other in vertical direction developer
position)
A0707 11 Reagent mixer
cannot find home Vertical position Restore failure. If
position when sensor failure. failed for 3 times,
moving Obstruction exists contact the
vertically(in in vertical direction developer
reaction disk)
A0708 9 Reagent mixer is
Lowering down If auto reset fails,
not in vertical
at current restore failure. If
home position.
position is not failed for 3 times,
Current position is
allowed(other contact the
not proper for
position) developer
lowering down
A0709 11 Reagent mixer is
Lowering down If auto reset fails,
not in vertical
at current restore failure. If
home position.
position is not failed for 3 times,
Current position is
allowed(in contact the
not proper for
reaction disk) developer
lowering down
10-32 10 Troubleshooting
Error Level Error Message Probable Causes Corrective Actions
Code
A0710 9 Switch off analyzing
unit power and
Wrong sensor
switch on again.
status when
Restore failure on
reagent mixer Rotational position
Utilities-->Daily
moves sensor failure
Maint. page. If this
horizontally(other
message appears
position)
for 3 times, contact
the developer
A0711 11 Switch off analyzing
unit power and
Wrong sensor
switch on again.
status when
Restore failure on
reagent mixer Rotational position
Utilities-->Daily
moves sensor failure
Maint. page. If this
horizontally(in
message appears
reaction disk)
for 3 times, contact
the developer
A0712 9 Reagent mixer
Rotational position
cannot find home Restore failure. If
sensor failure.
position when failed for 3 times,
Obstruction exists
moving contact the
in horizontal
horizontally(other developer
direction
position)
A0713 11 Reagent mixer
Rotational position
cannot find home Restore failure. If
sensor failure.
position when failed for 3 times,
Obstruction exists
moving contact the
in horizontal
horizontally(in developer
direction
reaction disk)
A0714 9 Reagent mixer is
not in horizontal If auto reset fails,
Rotating at
home position. restore failure. If
current height is
Current height is failed for 3 times,
not allowed(other
not proper for contact the
position)
rotation(highest developer
position)
A0715 11 Reagent mixer is
not in horizontal If auto reset fails,
Rotating at
home position. restore failure. If
current height is
Current height is failed for 3 times,
not allowed(in
not proper for contact the
reaction disk)
rotation(highest developer
position)
A0716 11 Switch off analyzing
unit power and
switch on again.
Execution result Instruction Restore failure on
is not received in execution is timed Utilities-->Daily
given time out Maint. page. If this
message appears
for 3 times, contact
the developer
10 Troubleshooting 10-33
Error Level Error Message Probable Causes Corrective Actions
Code
A0717 11 Switch off analyzing
unit power and
switch on again.
Restore failure on
No response, or Instruction execute
Utilities-->Daily
response error error
Maint. page. If this
message appears
for 3 times, contact
the developer
10-34 10 Troubleshooting
Error Level Error Message Probable Causes Corrective Actions
Code
A0805 11 Switch off analyzing
unit power and
Wrong sensor
switch on again.
status when
Restore failure on
sample mixer Vertical position
Utilities-->Daily
moves sensor failure
Maint. page. If this
vertically(in
message appears
reaction disk)
for 3 times, contact
the developer
A0806 8 Sample mixer
cannot find home Vertical position Restore failure. If
position when sensor failure. failed for 3 times,
moving Obstruction exists contact the
vertically(other in vertical direction developer
position)
A0807 11 Sample mixer
cannot find home Vertical position Restore failure. If
position when sensor failure. failed for 3 times,
moving Obstruction exists contact the
vertically(in in vertical direction developer
reaction disk)
A0808 8 Sample mixer is
Lowering down If auto reset fails,
not in vertical home
at current restore failure. If
position. Current
position is not failed for 3 times,
position is not
allowed(other contact the
proper for lowering
position) developer
down
A0809 11 Sample mixer is
Lowering down If auto reset fails,
not in vertical home
at current restore failure. If
position. Current
position is not failed for 3 times,
position is not
allowed(in contact the
proper for lowering
reaction disk) developer
down
A0810 8 Switch off analyzing
unit power and
Wrong sensor
switch on again.
status when
Restore failure on
sample mixer Rotational position
Utilities-->Daily
moves sensor failure
Maint. page. If this
horizontally(other
message appears
position)
for 3 times, contact
the developer
A0811 11 Switch off analyzing
unit power and
Wrong sensor
switch on again.
status when
Restore failure on
sample mixer Rotational position
Utilities-->Daily
moves sensor failure
Maint. page. If this
horizontally(in
message appears
reaction disk)
for 3 times, contact
the developer
A0812 8 Sample mixer
Rotational position
cannot find home Restore failure. If
sensor failure.
position when failed for 3 times,
Obstruction exists
moving contact the
in horizontal
horizontally(other developer
direction
position)
10 Troubleshooting 10-35
Error Level Error Message Probable Causes Corrective Actions
Code
A0813 11 Sample mixer
Rotational position
cannot find home Restore failure. If
sensor failure.
position when failed for 3 times,
Obstruction exists
moving contact the
in horizontal
horizontally(in developer
direction
reaction disk)
A0814 8 Sample mixer is
not in horizontal If auto reset fails,
Rotating at
home position. restore failure. If
current height is
Current height is failed for 3 times,
not allowed(other
not proper for contact the
position)
rotation(highest developer
position)
A0815 11 Sample mixer is
not in horizontal If auto reset fails,
Rotating at
home position. restore failure. If
current height is
Current height is failed for 3 times,
not allowed(in
not proper for contact the
reaction disk)
rotation(highest developer
position)
A0816 11 Switch off analyzing
unit power and
switch on again.
Execution result Instruction Restore failure on
is not received in execution is timed Utilities-->Daily
given time out Maint. page. If this
message appears
for 3 times, contact
the developer
A0817 11 Switch off analyzing
unit power and
switch on again.
Restore failure on
No response, or Instruction execute
Utilities-->Daily
response error error
Maint. page. If this
message appears
for 3 times, contact
the developer
10-36 10 Troubleshooting
Error Level Error Probable Causes Corrective Actions
Code Message
A0902 0 Reset analyzing unit
power and restart
operating software.
Instruction Instruction
Then retry this
parameter parameter does not
instruction. If this
error comply with protocol
message appears for 3
times, contact the
developer
A0903 0 Reset analyzing unit
power and restart
Unit is busy. operating software.
No execute Instruction conflicts. Then retry this
condition Instruction buffer is instruction. If this
full message appears for 3
times, contact the
developer
A0904 0 Reset hardware on
Utilities-->Daily Maint.
Reaction page and restart
Reaction disk
disk reaction disk
temperature
temperature temperature control. If
collection error
is too high this message appears
for 3 times, contact the
developer
A0905 0 Reset hardware on
Utilities-->Daily Maint.
page and restart
Wash Preheat
reaction disk
solution is temperature
temperature control. If
too hot collection error
this message appears
for 3 times, contact the
developer
A0906 0 Reset hardware on
Utilities-->Daily Maint.
Reaction page and restart
Reaction disk
disk reaction disk
temperature
temperature temperature control. If
collection error
is fluctuated this message appears
for 3 times, contact the
developer
A0907 0 Reset hardware on
Utilities-->Daily Maint.
Wash page and restart
Preheat
solution reaction disk
temperature
temperature temperature control. If
collection error
is fluctuated this message appears
for 3 times, contact the
developer
A0908 0 Reset hardware on
Utilities-->Daily Maint.
Reaction
page and restart
disk Reaction disk
reaction disk
temperature temperature
temperature control. If
control is collection error
this message appears
switched off
for 3 times, contact the
developer
10 Troubleshooting 10-37
Error Level Error Probable Causes Corrective Actions
Code Message
A0909 0 Reset hardware on
Utilities-->Daily Maint.
Preheat page and restart
Preheat
temperature reaction disk
temperature
control is temperature control. If
collection error
switched off this message appears
for 3 times, contact the
developer
A0910 0 Reset hardware on
Reaction
Utilities-->Daily Maint.
disk
Reaction disk page and restart
temperature
temperature reaction disk
is not
collection error. temperature control. If
steady in
Heater control error this message appears
specified
for 3 times, contact the
time
developer
A0911 0 Reset hardware on
Wash
Utilities-->Daily Maint.
solution
Preheat page and restart
temperature
temperature reaction disk
is not
collection error. temperature control. If
steady in
Heater control error this message appears
specified
for 3 times, contact the
time
developer
A0912 0 Refrigerator
Fan circuit failed. Contact equipment
fans are
Fan is damaged developer
abnormal
A0913 11 Switch off analyzing unit
power and switch on
Execution
Instruction again. Restore failure on
result is not
execution is timed Utilities-->Daily Maint.
received in
out page. If this message
given time
appears for 3 times,
contact the developer
A0914 11 Switch off analyzing unit
power and switch on
No
again. Restore failure on
response, Instruction execute
Utilities-->Daily Maint.
or response error
page. If this message
error
appears for 3 times,
contact the developer
A0915 0 Reaction
disk Reaction disk
temperature temperature sensor Contact equipment
sensor is is disconnected or developer
not damaged
connected
A0916 0 Preheat
Preheat
temperature
temperature sensor Contact equipment
sensor is
is disconnected or developer
not
damaged
connected
A0917 0 Pressure
Fan control circuit
machine Contact equipment
error, or fan is
fans developer
damaged
abnormal
10-38 10 Troubleshooting
10.2.2.11 Wash Unit
Error Level Error Probable Causes Corrective Actions
Code Message
10 Troubleshooting 10-39
Error Level Error Probable Causes Corrective Actions
Code Message
A1008 17 High-conce
Liquid level sensor is
ntration Empty
abnormal. Waste
waste high-concentration
buffer is not emptied
buffer is too waste buffer
in time
high
A1009 17 Low-conce
Liquid level sensor is
ntration Empty
abnormal. Waste
waste low-concentration waste
buffer is not emptied
buffer is too buffer
in time
high
A1010 17 Waste pump
abnormal. Liquid
Waste
sensor abnormal.
buffer level Troubleshoot failed part
External sewer
is too high
blocked. Tubing fell
off
A1011 17 Liquid sensor
abnormal. Inlet valve Troubleshoot failed part.
abnormal. Water Execute "System
Water tank
treatment system Prime" and select
level is too
abnormal. Water "Water
low
supply unit abnormal. Tank/Probes/Mixers
10psig pressure Prime"
abnormal
A1012 17 Liquid sensor
abnormal. Inlet valve
Water tank abnormal. Water
level is too treatment system Troubleshoot failed part
high abnormal. Water
supply unit abnormal.
Main filter abnormal
A1013 10 Valve abnormal. No Troubleshoot failed part.
Diluted diluted wash solution. Execute "System
wash tank Water tank level too Prime" and select
is too low low. Liquid level "Diluted Wash Solution
sensor abnormal Tank Prime"
A1014 0 Valve abnormal. No
Concentrat concentrated wash
Troubleshoot failed part.
ed wash solution. Water tank
Execute "Concentrated
tank is too level too low. Liquid
Wash Solution Refill"
low level sensor
abnormal
A1015 11 Restore failure on
Pump/valv Utilities-->Daily Maint.
Pump failure. Valve
e goes page. If this message
failure
wrong appears for 3 times,
contact the developer
10-40 10 Troubleshooting
Error Level Error Probable Causes Corrective Actions
Code Message
10 Troubleshooting 10-41
Error Level Error Probable Causes Corrective Actions
Code Message
A1021 12 Wash
solution
flow for Exterior wash valve
Troubleshoot failed part
probe/bar abnormal
exterior is
abnormal
A1022 12 Wash
probe/tubing/check
Cuvette valve clogged.
Troubleshoot failed part
overflow Vacuum pressure
abnormal. Wash unit
abnormal
A1023 12 Wash probe clogged.
No wash Connector leakage.
solution is 10psig pressure
dispensed abnormal. Water tank Troubleshoot failed part
into level too low. Preheat
cuvettes module abnormal.
Loose connector
A1024 12 Tubing pressure not
released completely.
Air pump
High pressure Troubleshoot failed part
cannot start
protection of pressure
switch started
A1025 12 Dilution
Pressure of diluted
cannot
wash solution tank is
proceed Troubleshoot failed part
not released
successfull
completely
y
A1026 12 Water
accumulate Waste buffer level too
Troubleshoot failed part.
s in reagent high. Waste tubing
Arrange waste tubing
disk and clogged
wash well
A1027 0 Check the inlet tubing
Water tank
Water flow is weak, or connection. If error
cannot be
floater goes wrong remain, contact the
filled
developer
A1028 11 Pressure
Sensor is damaged
sensor Contact the developer
or disconnected
check error
A1029 11 Pressure
sensor Sensor goes wrong
Contact the developer
calibration during maintenance
error
10-42 10 Troubleshooting
Error Level Error Probable Causes Corrective Actions
Code Message
10 Troubleshooting 10-43
Error Level Error Probable Causes Corrective Actions
Code Message
A1038 12 Pressure of
Adjust the protection
25psign
Protection valve is valve to increase
gauge is
not adjusted correctly pressure to
within
25psig(0.172MPa)
27-35psig
A1039 12 Shut down the analyzer,
Pressure of
clean the tubing, check
25psign Protection valve and
the protection valve,
gauge is one-way valve are
pressure switch, power
greater blocked. Tubing is
supply and power cord.
than 35psig clogged or broken
Then start up the
at any time
analyzer
A1040 12 Vacuum
Turn off air pump and
pressure is
Air pump is blocked turn on again. If the
greater
and cannot work error remains, shut
than
down the analyzer
-0.5psig
A1041 12 Vacuum
pressure is
greater Tubing are leaking or Shut down the analyzer
than not connected and check the fluidic
-10psig in properly tubing connection
standby
status
A1042 12 Pressure of
Turn off air pump and
10psig
Air pump is blocked turn on again. If the
gauge is
and cannot work error remains, shut
less than
down the analyzer
1psig
A1043 12 Pressure of
10psig
gauge is Tubing are leaking or Shut down the analyzer
less than not connected and check the fluidic
8psig in properly tubing connection
standby
status
A1044 12 Pressure of Shut down the analyzer,
Inlet valve cannot be
10psign check the water supply
turned off. Water tank
gauge is and floater of water
overflow. Pressure
greater tank. Replace the inlet
too high
than 13psig valve if necessary
A1045 12 Pressure of
Turn off air pump and
5psig
Air pump is blocked turn on again. If the
gauge is
and cannot work error remains, shut
less than
down the analyzer
1psig
A1046 12 Pressure of
5psig
gauge is Tubing are leaking or Shut down the analyzer
less than not connected and check the fluidic
3psig in properly tubing connection
standby
status
10-44 10 Troubleshooting
Error Level Error Probable Causes Corrective Actions
Code Message
A1047 12 Result
error:
Diluted
wash
Not enough pressure Contact the developer
solution
tank
emptying
failed
A1048 12 Result
error:
System
Tubing clogged Troubleshoot the tubing
pressure
releasing
failed
A1049 12 Result
error:
System
Tubing leaking Troubleshoot the tubing
pressure
establishin
g failed
A1050 12 Result
error:
Diluted
Pressure cannot
wash Restart the vacuum and
reach the expected
solution pressure gauges
value
tank
priming
failed
A1051 12 Result
error:
System
Tubing clogged Troubleshoot the tubing
vacuum
releasing
failed
A1052 12 Result
error:
System
Tubing leaking Troubleshoot the tubing
vacuum
establishin
g failed
10 Troubleshooting 10-45
Error Level Error Probable Causes Corrective Actions
Code Message
A1103 14 Power switched off.
Communication
Reconnect to power.
failed. Serial cable
ISE unit Reconnect serial cable.
damaged or
does not Perform ISE initialization.
unconnected. Module
respond Contact the developer to
connector damaged.
replace board
Board elements
damaged
A1104 14 Reinstall sensor. Replace
Electrode installation calibrant B and rerun, if
incorrect. Calibrator still low, replace calibrant
expired. Electrode A and rerun. Replace
Na+
degenerated. Bubbles failed sensor and rerun.
electrode
in reference Reinstall electrode,
slope is out
electrode. Reference eliminate bubbles and
of standard
electrode damaged. calibrate. Replace
range
Electrodes interfered. reference or Na+
Module or tubing electrode and rerun.
temperature above 37 Monitor temperature, if too
high, relocate equipment
A1105 14 Reinstall sensor. Replace
Electrode installation calibrant B and rerun, if
incorrect. Calibrator still low, replace calibrant
expired. Electrode A and rerun. Replace
K+
degenerated. Bubbles failed sensor and rerun.
electrode
in reference Reinstall electrode,
slope is out
electrode. Reference eliminate bubbles and
of standard
electrode damaged. calibrate. Replace
range
Electrodes interfered. reference or K+ electrode
Module or tubing and rerun. Monitor
temperature above 37 temperature, if too high,
relocate equipment
A1106 14 Reinstall sensor. Replace
Electrode installation calibrant B and rerun, if
incorrect. Calibrator still low, replace calibrant
expired. Electrode A and rerun. Replace
Cl-
degenerated. Bubbles failed sensor and rerun.
electrode
in reference Reinstall electrode,
slope is out
electrode. Reference eliminate bubbles and
of standard
electrode damaged. calibrate. Replace
range
Electrodes interfered. reference or Cl- electrode
Module or tubing and rerun. Monitor
temperature above 37 temperature, if too high,
relocate equipment
A1107 14 Electrode installation Reinstall sensor. Replace
incorrect. Calibrator calibrant B and rerun, if
expired. Electrode still low, replace calibrant
Li+ A and rerun. Replace
degenerated. Bubbles
electrode failed sensor and rerun.
in reference
slope is out Reinstall electrode,
electrode. Reference
of standard eliminate bubbles and
electrode damaged.
range calibrate. Replace
Electrodes interfered.
Module or tubing reference or Li+ electrode
temperature above 37 and rerun. Monitor
temperature, if too high,
10-46 10 Troubleshooting
Error Level Error Probable Causes Corrective Actions
Code Message
relocate equipment
A1108 14 Replace electrode and
Na+ Electrode rerun. Check for electrical
electrode degenerated. Noise noise. If board element is
noise error spike interference damaged, replace the
board
A1109 14 Replace electrode and
K+ Electrode rerun. Check for electrical
electrode degenerated. Noise noise. If board element is
noise error spike interference damaged, replace the
board
A1110 14 Replace electrode and
Cl- Electrode rerun. Check for electrical
electrode degenerated. Noise noise. If board element is
noise error spike interference damaged, replace the
board
A1111 14 Replace electrode and
Li+ Electrode rerun. Check for electrical
electrode degenerated. Noise noise. If board element is
noise error spike interference damaged, replace the
board
A1112 14 Replace reference
Reference electrode electrode and rerun.
Na+, K+,
degenerated. Check for electrical noise.
Cl-, Li+
Environment Check unit grounding. If
electrodes
interfered by electrical board element is
noise error
noise spike damaged, replace the
board
A1113 14 Replace failed electrode
Electrode and run. Check calibrant A
Na+
degenerated. New channel and recalibrate. In
electrode
electrode or new case of new electrode,
drift
calibrant A is used drift may occur, wait for 15
minutes and test again
A1114 14 Replace failed electrode
Electrode and run. Check calibrant A
K+
degenerated. New channel and recalibrate. In
electrode
electrode or new case of new electrode,
drift
calibrant A is used drift may occur, wait for 15
minutes and test again
A1115 14 Replace failed electrode
Electrode and run. Check calibrant A
Cl-
degenerated. New channel and recalibrate. In
electrode
electrode or new case of new electrode,
drift
calibrant A is used drift may occur, wait for 15
minutes and test again
A1116 14 Electrode Replace failed electrode
Li+ and run. Check calibrant A
degenerated. New
electrode channel and recalibrate. In
electrode or new
drift case of new electrode,
calibrant A is used
drift may occur, wait for 15
10 Troubleshooting 10-47
Error Level Error Probable Causes Corrective Actions
Code Message
minutes and test again
A1117 14 Replace reference
Reference electrode
electrode and rerun.
Na+, K+, degenerated.
Check for electrical noise.
Cl-, Li+ Environmental electric
If board element is
electrodes pulse. New electrode
damaged, replace the
drift or new calibrant A is
board. Check calibrant A
used
channel and recalibrate
A1118 14 Replace failed electrode
Na+ Electrode and run. Check calibrant A
electrode degenerated. New channel and recalibrate. In
voltage electrode or new case of new electrode,
overflow calibrant A is used drift may occur, wait for 15
minutes and test again
A1119 14 Replace failed electrode
K+ Electrode and run. Check calibrant A
electrode degenerated. New channel and recalibrate. In
voltage electrode or new case of new electrode,
overflow calibrant A is used drift may occur, wait for 15
minutes and test again
A1120 14 Replace failed electrode
Cl- Electrode and run. Check calibrant A
electrode degenerated. New channel and recalibrate. In
voltage electrode or new case of new electrode,
overflow calibrant A is used drift may occur, wait for 15
minutes and test again
A1121 14 Replace failed electrode
Li+ Electrode and run. Check calibrant A
electrode degenerated. New channel and recalibrate. In
voltage electrode or new case of new electrode,
overflow calibrant A is used drift may occur, wait for 15
minutes and test again
A1122 14 Replace reference
Reference electrode
Na+, K+, electrode and rerun.
degenerated.
Cl-, Li+ Check for electrical noise.
Environmental electric
electrodes If board element is
pulse. New electrode
voltage damaged, replace the
or new calibrant A is
overflow board. Check calibrator A
used
channel and recalibrate
A1123 3 If sample is less than 70µl,
Insufficient sample.
increase sample volume.
Sample not aspirated
Fill sufficient sample in
Air in to measurement
tube. In case of wrong
sample chamber. Tubing
electrode installation,
aged. Pump tubing
install again. Replace the
clogged or too long
tubing
A1124 14 Calibrant A depleted. Check spring/sealing ring.
Tubing unconnected. Ensure all electrodes/O
Calibrant A pump rings are correct. Launch a
Air in failure. Tubing wash procedure.
calibrant A clogged/cracked/bent. Disassemble the unit and
Fibrin and salt in reinstall sensor. Replace
electrode tubing. bubble detector, waste
Bubble detector pump, or calibrant A and
10-48 10 Troubleshooting
Error Level Error Probable Causes Corrective Actions
Code Message
failure. Waste pump recalibrate.
failure Reconnect/replace tubing.
Check electrical
connection. Replace pump
housing, motor, or tubing
A1125 14 In case of wrong electrode
installation, check spring
and sealing ring. Ensure
Air in calibrant B.
all electrodes and O rings
Fibrin and salt in
are installed correctly.
Air in electrode tubing.
Press Clean on water
calibrant B Bubble detector
treatment system to wash.
failure. Waste pump
Disassemble this unit and
failure
wash and reinstall sensor.
Replace bubble detector.
Replace waste pump.
A1126 14 In case of wrong electrode
installation, check spring
and sealing ring. Ensure
Air in calibrant B and
all electrodes and O rings
A. Fibrin and salt in
are installed correctly.
Air in electrode tubing.
Press Clean on water
cleaner Bubble detector
treatment system to wash.
failure. Waste pump
Disassemble this unit and
failure
wash and reinstall sensor.
Replace bubble detector.
Replace waste pump.
A1127 14 Calibrator A/B
No fluid in Replace reagent package.
depleted. No sample
tubing Check the tubing
or cleaning solution
A1128 14 Instruction Instruction format
Please contact the
execute error, or parameter
developer
error error
A1129 14 Calibrations ISE unit cannot store
Recalibrate
saving error calibration results
A1130 14 Bubble
Bubble detector is Replace the bubble
detector
damaged detector
failure
A1131 14
Calibrate repeatedly. If
Electrode slope is out problem remains, reinstall
Calibration
of range during failed electrode. If problem
failed
calibration still remains, replace the
electrode
10 Troubleshooting 10-49
Error Level Error Probable Causes Corrective Actions
Code Message
A1132 14 If maintaining, send
instruction again, if testing,
Instruction Instruction format rerun. Otherwise restart
execute error. Parameter error. operation unit and
error No execute condition analyzing unit. If this
problem remains, contact
the developer
10-50 10 Troubleshooting
Error Level Error Probable Causes Corrective Actions
Code Message
A1137 0 Reinstall sensor. Replace
Electrode installation calibrant B and rerun, if
incorrect. Calibrator still low, replace calibrant
expired. Electrode A and rerun. Replace
Cl-
degenerated. Bubbles failed sensor and rerun.
electrode
in reference Reinstall electrode,
slope is out
electrode. Reference eliminate bubbles and
of standard
electrode damaged. calibrate. Replace
range
Electrodes interfered. reference or Cl- electrode
Module or tubing and rerun. Monitor
temperature above 37 temperature, if too high,
relocate equipment
A1138 0 Reinstall sensor. Replace
Electrode installation calibrant B and rerun, if
incorrect. Calibrator still low, replace calibrant
expired. Electrode A and rerun. Replace
Li+
degenerated. Bubbles failed sensor and rerun.
electrode
in reference Reinstall electrode,
slope is out
electrode. Reference eliminate bubbles and
of standard
electrode damaged. calibrate. Replace
range
Electrodes interfered. reference or Li+ electrode
Module or tubing and rerun. Monitor
temperature above 37 temperature, if too high,
relocate equipment
10.2.2.13 Others
Error Level Error Probable Causes Corrective Actions
Code Message
10 Troubleshooting 10-51
11 Calculation Methods
Endpoint
Fixed-time
Kinetic
11.1.1 Endpoint
The endpoint or, more correctly, equilibrium method, is most ideal. The reaction
reaches equilibrium after a period of time. Since the equilibrium constant is very large,
it can be considered that all substrates (analytes) have changed into products, and
absorbance of the reaction liquid does not change any more. The absorbance
change is directly proportional to the analytes concentration.
As shown in Figure 11-1, R1 is the time when reagent is dispensed and S when
sample is dispensed. From L to M the reaction reaches equilibrium and the
absorbance reading is taken. The reagent blank is tested during N and P.
11.1.1.2 Double-reagent
Figure 11-2 Double-reagent Endpoint Reaction Curve
As shown in Figure 11-2, R1, S and R2 are the time when first reagent, sample and
second reagent are respectively dispensed. From L to M the reaction reaches
equilibrium and the absorbance reading is taken. The sample blank is tested during N
and P.
To calculate reaction absorbance Ai, follow the relevant steps stated in 11.1.1.1
Single-reagent.
To calculate reagent blank absorbance Ab, follow the relevant steps stated in
11.1.1.1 Single-reagent.
Calculate the reaction response using the following equation:
As shown in Figure 11-3 and Figure 11-4, R1, S, R2, R3 and R4 are the time when
first reagent, sample, second reagent, third reagent and fourth reagent are
respectively dispensed. From L to M the reaction reaches equilibrium and the
absorbance reading is taken. The reagent blank is tested during N and P.
To calculate reaction absorbance Ai, follow the relevant steps stated in 11.1.1.1
Single-reagent.
To calculate reagent blank absorbance Ab, follow the relevant steps stated in
11.1.1.1 Single-reagent.
Reaction response calculation:
Triple-reagent R = Ai − k 3 Ab or R = Ai (reagent blank time is 0)
analysis
VR1 + VS + VR2
k3 = is a volume correction factor
VR1 + VS + VR2 + VR3
for triple-reagent analysis. VR1, VS, VR2 and VR3 are
volumes of first reagent, sample, second reagent and third
reagent.
11.1.2 Fixed-time
For the fixed-time reaction method, namely, first-order kinetic method or initial rate
method, the reaction velocity (v) within a specific period, is directly proportional to the
substrate concentration [S], that is, v=k[S]. As the substrate is consumed
continuously, the reaction velocity becomes smaller and smaller, and so does the
absorbance change rate. It takes much time for such a reaction to reach equilibrium.
Theoretically, the absorbance reading can be taken at any time. The reaction can,
however, become steady only after a lag because it is complicated at the beginning
and there are miscellaneous reactions due to complex serum compositions.
For any first order reaction, the substrate concentration [S] at given time since
reaction starts is obtained by the following formula:
[S ] = [S 0 ]× e − kt .
Where,
− ∆[ S ]
[ S 0] = − kt1 − kt 2
e −e
That is, within a fixed time interval, the change in substrate concentration is directly
proportional to its initial concentration. This is the general property of first-order
reactions. Within this interval, absorbance change is directly proportional to the
analytes concentration.
Substrate depletion can be checked in fixed-time reaction, and corresponding flag will
be marked in case of substrate depletion.
As shown in Figure 11-5, R1 is the time when first reagent is dispensed and S when
sample is dispensed. The absorbance readings are respectively taken at L and M.
11.1.2.2 Double-reagent
Figure 11-6 Double-reagent Fixed-time Reaction Curve
As shown in Figure 11-6, R1, S and R2 are the time when first reagent, sample and
second reagent are respectively dispensed. The absorbance readings are
respectively taken at L and M. The reagent blank is tested at N and P.
VR1 + VS
Where, k2 = is a volume correction factor for double-reagent
VR1 + VS + VR 2
analysis. VR1, VS and VR2 are volumes of first reagent, sample and second reagent.
As shown in Figure 11-7 and Figure 11-8, R1, S, R2, R3 and R4 are the time when
first reagent, sample, second reagent, third reagent and fourth reagent are
respectively dispensed. The absorbance readings are respectively taken at L and M.
The reagent blank is tested during N and P
VR1 + VS + VR2
Where, k3 = is a volume correction factor for
VR1 + VS + VR2 + VR3
triple-reagent analysis. VR1, VS, VR2 and VR3 are volumes of first reagent,
sample, second reagent and third reagent.
VR1 + VS + VR2 + VR3
k4 = is a volume correction factor for
VR1 + VS + VR2 + VR3 + VR4
quadruple-reagent analysis. VR1, VS, VR2, VR3 and VR4 are volumes of first
reagent, sample, second reagent, third reagent and fourth reagent.
11.1.3 Kinetic
For the kinetic method, namely, zero-order kinetic or continuous-monitoring method,
the reaction velocity is not related to substrate concentration and remains constant
during the reaction process. As a result, for a given wavelength, the absorbance of
the analytes changes evenly, and the change rate (∆A/min) is directly proportional to
the activity or concentration of the analytes. The kinetic method is usually used to
measure enzyme activity.
In fact, it is impossible for the substrate concentration to be high enough, and the
reaction will be no longer a zero-order reaction when the substrate is consumed to a
certain degree. Therefore, the theory only stands within certain period. In addition,
the reaction can become steady only after a certain period of time, because the
reaction is complicated at the beginning and there are miscellaneous reactions due to
complex serum compositions.
You should determine the linearity range within the reaction time other than the
reagent blank period.
Figure 11-10 shows the linearity range determination process for increased reaction.
In decreased reaction, the “≤” in Figure 11-10 should be changed into “≥”.
As shown in Figure 11-12, R1 is the time when first reagent is dispensed and S when
sample is dispensed. The absorbance readings are taken during L and M.
⊿ is the absorbance change rate per minute (slope of reaction curve) during L and
M and calculated by the least squares method.
Double-reagent
Figure 11-13 Double-reagent Kinetic Reaction Curve
As shown in Figure 11-13, R1, S and R2 are the time when first reagent, sample and
second reagent are respectively dispensed. The absorbance readings are taken
during L and M. The reagent blank is tested during N and P.
Triple- or Quadruple-reagent
Figure 11-14 Triple-reagent Kinetic Reaction Curve
As shown in Figure 11-14 and Figure 11-15, R1, S, R2, R3 and R4 are the time when
first reagent, sample, second reagent, third reagent and fourth reagent are
respectively dispensed. The absorbance readings are taken during L and M. The
reagent blank is tested during N and P.
∆A f − ∆Ab
Linearity= × 100 < Linearity Limit
∆Au ,v
Where, ∆A f , ∆Ab and ∆Au ,v are the absorbance change rates at the beginning
and end of reaction, and of all measuring points. These three values are calculated
based on the number of measuring points within the linearity range.
During the high-activity enzyme test, the substrate may be depleted quickly and the
reaction curve will appear obviously nonlinear (a smooth curve). If measurement is
performed by the ordinary procedure, the “No linear interval” alarm will be triggered,
reminding user to rerun the test after diluting the sample. This will more or less bring
troubles to user.
The BS-400/BS-420 provides the enzyme linearity range extension function, which is
introduced as follows:
When the number of measuring points(N) within the linearity range is less than 2, the
enzyme linearity range extension can be implemented.
When Antigen is selected, first edit box of ABS field and the Q3/Q4 fields on the
Basics window of the Test page are not available.
170> = q2≥102>q1≥Reaction end point. 102 is the last measuring point before
sample is added again.
Sample PC=Aq2-k×Aq1,
The reaction rate method is based on the specified time, in which antibody excess
reaction can reach equilibrium (Figure11-19) but antigen excess reaction cannot
(Figure11-20). Prozone check is performed using the following parameters:
(1) End point absorbance A is less than absorbance low limit in increased
reaction or greater than that in decreased reaction;
(2) Absolute value of response R is greater than RCMAX (absolute value of
response of most-concentrated calibrator).
Serum index is the level of hemolysis, icterus and lipemia in serum samples, and can
be employed to judge whether the sample can be used or a sample blank is needed.
Sample: Serum;
You should enter the serum factors (A, B, C, D, E, F) and thresholds for lipemia,
hemolysis and icterus.
Six wavelengths (n=450, 505, 570, 605, 670, 700) are selected to determine the
serum index.
Three reference solutions of lipemia, hemolysis and icterus are configured with concentration
of CL, CH, and CI respectively. Six serum index wavelengths provided by BS series are applied
to determine the serum factors.
A450 − A505
D= .
CI