Hitachi Ultra High Performance Liquid Chromatograph

HITACHI UHPLC
supports cutting edge analysis
ChromasterUltra Rs,, an ultra high performance liquid chromatograph, provides the ultimate in high performance,
high resolution, and high sensitivity analysis that are becoming requirements for research and development in
pharmaceutical and chemical fields. HITACHI UHPLC supports cutting edge studies including the high resolution
analysis of chemically-similar substances and impurities in synthetic compounds and the high sensitivity detection
of impurities such as harmful substances that are becoming increasingly relevant in our daily lives.

Ultra High-Speed Analysis

World's highest range of the pressure resistance, which is 140 MPa, not only supports high-speed analysis but also
gives selectivity of mobile phase which likely causes of higher pressure. This delivers more variety of analysis.

High Resolution Analysis

●The newly developed high resolution column (LaChromUltra Ⅱ ODS C18, particle size of 1.9 μm, 250 mm), has a
high number of theoretical plates (50,000), and high pressure resistance,, taking ultra high resolution analysis
to the next level.
●A newly designed 6170 Binary Pump with independent variable stroke plungers ensures solvent delivery with higher mixing
performance and stability as it employs the low-volume double corkscrew mixer in addition to its unique LBT*1 control.
* The above photo includes options and
●The standard installation of the 6430 Diode Array Detector includes a capillary type total reflection flow cell unit
user-preparation items.
(light path length: 10 mm). The excellent control of diffusion outside the column contributes to higher resolution
analysis.

*1 LBT：Liquid Beat Technology

Peptide mapping of digested protein
(1) LaChrom Ⅱ C18
4.6 × 250 mm (5 μm)
Peak capacity (4σ) 264
100.00
mAU

50.00

By using the NEW 250 mm long column with 1.9 μm particles, the number of
0.00
theoretical plates and peak capacity can be improved.
25.00 50.00
With the high pressure performance, it is possible to achieve about 1.4
60 min times higher peak capacity in half the time compared to the analysis by
using a column with 5 μm particles even if the column lengths are the
(2) LaChromUltra Ⅱ C18 same.
3.0 × 250 mm (1.9 μm)
400.00 Peak capacity (4σ)   368

Compared to the chromatogram

using a column with 5 μm
particle size, 1.4 times the peak
200.00
mAU

capacity can be realized, [LC Conditions]

reducing analysis time to Eluent : (A)0.1%-TFA/H2O Column Temp. : 40℃
0.00
30 min. (B)0.1%-TFA/CH3CN Detection : 215 nm
Gradient : (1)5% B(0 min) → 45% B(60 min) Injection vol. : (1)10 μL(about 100 μg)
(2)5% B(0 min) → 45% B(30 min) (2) 5 μL(about 50 μg)
12.00
min
24.00
30 min Flow rate : (1)1.0 mL/min (2)0.85 mL/min

High Sensitivity Analysis

●By using the High Sensitivity flow cell unit for 6430 Diode Array Detector (light path length: 65 mm) (optional), excellent sensitivity can be obtained from the 6430
Diode Array Detector.
●The 6430 Diode Array Detector equipped with a new optical system achieves low noise and low drift which are key to high sensitivity analysis.
●The 6270 Autosampler, which allows a selection of multiple washing modes, is installed with an injection port reverse washing mechanism and can provide the
excellent carry over performance.
 Other Features to Assist Your Analysis
Operability
●The standard installation includes the auto-purge valve, solvent selector, and 6-channel degassing
unit. As a 3 liter waste solvent tank is installed within the main body, there is no need to keep a
separate space for a waste solvent tank. The MEM column fitting*2 (optional) provides high
pressure resistance while allowing column changes simply by hand tightening.

*2 MEM: Moment Enhancing Mechanism

MEM column fitting (optional)

Expandability
●The GUI controller (optional) is equipped with a color liquid crystal touch panel. It provides
excellent operability and access, and it can be used for all modules and operations such as
monitor and control. With the enhanced timer function, the instrument warm-up before analysis, the
shutdown and analytical run can all be automated by using the Wake up/Sleep function.

●The column control function (optional) enables log control for analytical columns and pre-columns
regardless of the column manufacturers. Functions such as tracking the column usage history and
alerting for the column lifetime can be used.

GUI Controller (optional)

Maintainability
●The plunger washing pump and the washing mechanism are included as standard features, so the
potential for damage to the plunger and plunger seal can be reduced.
●To prevent a sudden pressure load, the build-up flow system is included in the standard
installation.
●Maintenance such as plunger seal, flow cell and lamp replacement can be performed from the front side.

Lineup
●Diode array detector (DAD) system
Change of Flow Cell
●UV-VIS detector system

CAUTION: For correct operation, follow the instruction manual when using the instrument.
Specifications in this catalog are subject to change with or without notice, as Hitachi High-Technologies Corporation continues to develop the latest
technologies and products for our customers.
NOTICE: The system is For Research Use Only, and is not intended for any animal or human therapeutic or diagnostic use.
These data are an example of measurement; the individual values can not be guaranteed.

Tokyo, Japan
www.hitachi-hitec.com/global/science/
24-14, Nishi-shimbashi 1-chome, Minato-ku Tokyo 105-8717, Japan

For technical consultation before purchase, please contact:

contact@nst.hitachi-hitec.com

Printed in Japan (H) HTB-E104 2013.9