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VISIBLE and UV - VIS SPECTROPHOTOMETER
USER'S IUIAHUAL

{
working Environment and lnstalration Requirements
t Rated voltage is 94V-240V AC, 50 / 60Hz*1H2.
O Rated voltage is 110 / 2z0v+10% AC,60 /50H2*1Hz (for model UV-vis cnly).
O lnstrument should be put in a dry room with temperature at 5'C-35'C, and relative humidity
is not than 85 %.
I The instrument should be placed on the soiid stable working platform, no strong
vibration or continuous vibration.
{l The room should no hydrogen sulfide, nii.ess acid fluoride and other eorrosive gasses.
O The instrument should stay awav from higr-intensitv magnetic field, electric field and
produced high-frequency \^/ave device.
O The instrument should avoid a direct hit by Sl-i-:3 wifld.
t The instrument should avoid strcnq sunshine.
I Grounding well is necessary.
t Warm up the host at least 3Onrins aiie. aci,,rer on.

Standard Configration nformation I

t Standard Configuration
1) Spectrophotometer
2) Power cable
3 ) User's manual & packing list

4) Certificate of quality checking

5) Dust Cover il5)
6) Glass cuvette 1cm (16)
i set
1pc
1pc
1pc
1pc
4 pcs
r \

7 t Quartz currette lcrn (!7) 2pcs (for selected model)

8) Cuvette i'ack 1cm 1 pc (installed)
9) Cuvette rack pull rod (19) 1pc (install before using)
10) Black cuvette for adjust 0%T (110) 1pc
11 ) Fuse (li 1) 2pcs
I Optional Aceessories
1 ) Tungsten halogen lamp (consumable)
2) Deuterium lamp (consumable) (for UV- Vis model)
3 ) Glass cuvette 0.5cm,1cm, 2cm, 3cm , 5cm
4) lcm cuvette rack
5 ) Scm cuvette rack ( adaptable for 1-5cm cuvette )
6 ) Quartz cuvette microscale, 1cm, 2cm, 3cm, 5cm (for UV-Vis modei)
10 ) Working curve maker and data processing software (except Visible model)

Please read operatG manual Garefrlly.

 .r.' i-,elft,
INDEX
Chapter 1 Applications and Specifications
Chapter 2 ' :
Function Details and Operate Guide '

Ghapter { Applieations and Specifications

Single beam visible spectrophotometer listed in this manual can be used for quantitative
measuring with Transmittance and Absorbance functions, it is one of the most important quality
control instruments widely used in chemical, pharmaceutical, biochemistry, metallurgy, light industry,
food processing, materials, environment protection, medi:al teting and the other fields.

Vis UV-Vis

Optical System single Beam with 1200 linesimm diffraction grating

Display LCD
Wavelength Range (nm)
340-1000 330-1000 I 325-1000 320- 1 000 200-1 000

tungsten-halogen
Lamp tungsten-halogen
deuterium
Detector
Silicon Photocell
Spectral Bandwidth 4nm 2nm 4nm 2nm

Skay Lisnt (%T@360nm) <0.'l <0.05

Wavelength Accuracy {nm) +1 +2 +1

!_2

Wavelength Reproducibiiity (nm) 1 0.5 1 0.5

Photometric Accu racy (%T) !1 10,5
Photometric Reprod ucibiliiy (%T) 0.5 0.2

Stability (Al3min) a0.002 +0.001

Photometric Range 0.0-200,0(7oT) / -0,301 -3{A y 0*9999(C}

Communication I nterface None RS232

Printer None Optional equipped optional

Quantative Software None optional

185-240 224 185-240

lnput Voltage (AC,V)
50Hz +1Hz 110 50Hz +1Hz

Dimensions {mm) 457 x342 x15A

Net Weight (kgs) 11
 Ghapter 2 Function Detalls and Operate Gulde

A FunEtion Details ', .';u';+':t'.f"itf

' t ''
* '. ''j

2: wavelength selectinglcrob and viewwindows

3: panel
4: cuvett€ rack pull rod

I Model -

10: fuse cover (replace fuse by removingiti

11: powerswitch
12t powercable
13: latel

16 17

L3: fuse cover (repiace fuse b1' rernoving it)

1,4: pollrel Su.itCh

i5, pr-irfer cai:le

16: RS232 interface (for using printer or software)

17: labei

18: por,r,'er switch

19: fuse cover (replace fuse by rernoving iti
Itl.
&V.
IlC,)?1
L\JLJA
irrl.r.(rr.r
U tIL 1 U\
T L

(for using printer or soffw,are)

21: power cable
22; lamp selecting sr,r.itch (,@3aOrun)
( for deuterium and tungsten-halogen larnp cutting)
23: iabel
 B Operate Guide
r Functions

5: this button is a cycling buttom for selecting measuring mode:

A default value is Abs (absorbtion), press one time rs Trans (transmittance)
A press tr.r,o times is Conc (concentration) , press t1-Lree hmes is Fact (factor)
6: 100i'r,T (adjust 100?tT) 7: 0,'oT {arljr.rst i-_ri:Tr

8: printout data through mini printer iol-.tiona1) via RS232 jnterface

9: function r.r.indor,r,s (urith light shorv) 26: LCD display winrlor,r,s
2* LeD dispia\. u,indora.'s
r $ample rosm

11. sample rack flred plate

25: 1cm cuvette rack (replaceable)
t. cuvette rack puil rocl
=.

r Steps

1. Power on, preheating 3Omins

2. Select wavelength by turning wavelength knob (2)
3. Press button Func (5) tillTrans mode lamp ligntend (9)
4. Put black cuvette (110) into cuvette rack (2S),enter optical bath by putling cuvette rack rod

5. J::ffilT:1#ffi dspay oo o or -oo o $

6. Put reference liquid sample into cuvette rack (2s),enter optical bath by pulling cuvette rack rod (4)
7. "
Press button Blank {6), BL will be shown for several seconds, then LCD will display 100.0"
8. Select measuring mode (Abs,Trans) by pressing button Func (5)
L Put liquid sample into cuvette rack (2S),enter light bath by pulling cuvette rack rodfrgfc
(4) ,close sample room cover
10" Get measuring data on LCD
11. Printout data by pressing button Enuprt (B) if (except vis modet)
Note: Step 4-7 should be operate again when change wavelength $r test interra-;' ,r...i ;: ,,,,:; , ,,;. ,:

Quantitative measuring is used to get sample concentration, standr:.r " :inpii:,.'

concentration valuer will be needed for making wo.king cLlrve.
o Steps:
1. Power on, preheating 3Omins
2. Select wavelength by turning wavelength knob (2)
3. Press button Func (5) till Trans mode lamp lignteno i9)
4. Put black cuvette (110) into cuvette rack (25) entei optica bath by pulling cuvette rack rod (4),
close sample room cover
5. Press button Zero (7\, till LCD display 00 tl .rr -r',
6. Put reference liquid sample into cuvette rack(25). enter optical bath by pulling cuvette rack rod (4)
7. Press button Blank (6), BL will be shorvn for several seconds, then LCD will display 100.0
B. Select measuring mode (Abs, Trans) by pressing button Func (5)
9. Put liquid standard sample with known concentration into cuvette rack (25),enter optical bath by
pulling cuvette rack rod (4), close sample room cover. get measuring data (Abs or Trans) and
record it on paper (for Vis model) or printout it through mini printer
10. Finish all standard liquid sample with known concentration measuring one by one just as steps
above, get measuring data on LCD and record data on paper (for Vis model) or printout them
through mini printer
11. Put liquid sample into cuvette rack (25), enter optical bath by pulling cuvette rack rod(4), close
sample room cover, get measuring data on LCD and record data
13. lnput all data to build a working curve by using excei, vertical ordinate value is concentration of
standard sample, horizontal ordinate value is Abs or Trans measured from instrument.
Note: four and above standard samples with gradient concentration value is necessary for better

Linear Accuracy.

Note: Effective linear concentration range is 1-1999, pls dilution samples if it is out of range.

a How to replace halogen-tungsten lamp

+ Power off
r Unscrew 1pc screw of wavelength knob,and take awa)' wavelengtlt knob

ffi
+ Remove 4pcs screws on the left and right side of instrument cover
 r Open instrument cover (care for connection cable)
+ Press the spring which fixed halogen{ungsten lamp lightly, pulithe hair:g*n irlrG$!r.,
ijliij;r.
out of the seat.

t lnsert the new halogen-tungsten lamp into the seat, and fix it with the spring.
o Watch the light spot emitted by the halogen-tungsten iar-np on the entrance slit,.check
whetherthe position is correct. lf ihe value on LCD lTrans) is unstable, adjustthe position
of lamp slight until the reading is stable.

a How to replace deuterium lamp (for UV -Vis model)

r Power off
. unscrew 1pc screw of wavelength hnob,and talre awa)' uaavelengilr knob

ffiffi
e Remove 4pcs screws on the left and right side of instrument cover
+ Open instrument cover (care for connection mble)
+ Loosen three color wires of deuterium lamp by screwdriver (pls remember connected positions of
color wires, this is very important ! ).
+ Take away deuterium lamp (together with the lamp holder) from the seat (this can be done only
by unscrewing the two bolts in the lamp holder), and loosen the clamp bolt of deuterium lamp.
+ Put on the new lamp, fix the deuterium lamp (together with the deuterium lamp holder) on the
seat, connect the colorwires (wires should be connected as before)
Caution: pls connect color wires just as before!
+ Adjust the deuterium lamp hole around 55mm away from the bottom plate, the position of light
hole is toward the spherical reflection minor in the lamp chamber
. Power on, keep lamp selecting switch at deuterium side.
+ Watch the light spot emitted by the deuterium lamp on the entrance siit, to check whether its
position is correct. lf the reading (Trans value) is unstable, readjust the position of the
deuterium lamp in a slight way until the reading is stable, and then tighten the clamp bolt.