Annals of Agri Bio Research

(ISSN: 0971-9660)
Volume 17, Issue 2, December 2012, Pages 87-92, Impact Factor: SJR-0.1, H- index-2,NAAS-3.5

In vitro plant regeneration in Salvadora oleoides

Decne from cotyledonary node
Singh, Narender.a , Laura, J.S.b, Kumar, Surender.b
a
Department of Botany, Kurukshetra University, Kurukshetra (Haryana), India
b
Department of Environmental Sciences, M. D. University, Rohtak-124001(Haryana), India

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Abstract
An efficient and reproducible protocol for micropropagation of Salvadora oleoides Decne has been
standardized. Seeds were germinated on half strength MS medium. Cotyledonary node explants (5-7 mm long)
excised from 12-15 days old aseptically grown seedlings were cultured on MS medium supplemented with
different concentrations of cytokinins alone and in combination with auxins. Maximum shoot proliferation
from single explant was obtained on MS medium fortified with 4.0 mg/1 BAP and 0.5 mg/1 NAA. In vitro
regenerated shoots induced roots on MS medium supplemented with 1.0 mg/1 NAA. The regenerants were
transferred to field conditions after acclimatization with 80% survival rate.

Author keywords
Auxins; Cotyledonary node; Cytokinins; Micropropagation; Salvadora oleoides

Indian Science abstract

ISA VOLUME:49 ISA ISSUE:19 DATE:01 /Oct /2013

Ann Agri Bio Res-

019819 Singh N;Laura J S;Surender Kumar
Botany Dep, Kurukshetra Univ, Kurukshetra, Haryana and Bio Science (EVS) Dep MDU Rohtak,Haryana.
In vitro plant regeneration in Salvadora oleoides decne from cotyledonary node , 2012,17(2),87-92