Beruflich Dokumente
Kultur Dokumente
Immunoglobulin
Contents
Introduction
Section Molecular Structure of Ig
Section Characteristics and Functions of the 5
Classes of Ig
Section Fc Receptors for Ab Molecules
Section Biological Activity of Ab
Section Immunogenicity of Ig
Section Artificial Ab
Concepts
Antibody (Ab): Glycoprotein molecules
that are produced by plasma cells and can
combine with the corresponding Ag
specifically are called Ab.
l 1~ l 4
k : l 20:1 (in mice); 2:1 (in human)
3. Two regions of each peptide chain
IgA
. Domains of Ig
1. Domain :
• L chain(2) : VL, CL
• H chain(4~5):
VH,
CH1, CH2, CH3
CH4(in IgM,IgE)
hinge region
3. Function of each
domain
• VH, VL: antigen-binding site
• CH1, CL: allogeneic marker
• CH2/CH3: complement-fixing site,
permeate placenta(IgG)
• CH3/CH4: cell-binding site
l Hinge region :flexible and suitable for CDR of
l Fragments:
2Fab:fragment antigen-binding
Fc:fragment crystallizable
l Function:
Fab: recognize and bind Ag
Fc:
(1) fix complement
(2) crossing the placenta
(3) bind to FcR in different cells
•
2. Digested by pepsin
• Position:
near the S-S bond of H inter-chains from the C end
• Fragments and function :
F(ab′)2: bind antigen(2 valence)
pFc′: no function
Significance
l Elucidating the relationships
between the structure and function
of Igs