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25/07/2018 ExPASy - PeptideMass

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PeptideMass

PeptideMass
The entered protein is: P69905

The selected enzyme is: Glu C (phosphate)

Maximum number of missed cleavages (MC): 0

All cysteines in reduced form.

Methionines have not been oxidized.

Displaying peptides with a mass bigger than 500 Dalton.

+
Using monoisotopic masses of the occurring amino acid residues and giving peptide masses as [M+H] .

You have selected HBA_HUMAN (P69905) from UniProtKB/Swiss-Prot:

Hemoglobin subunit alpha (Alpha-globin) (Hemoglobin alpha chain)

Chain Hemoglobin subunit alpha at positions 2 - 142 [Theoretical pI: 8.73 / Mw (average mass):
15126.36 / Mw (monoisotopic mass): 15116.88]
mass position #MC peptide sequence
3455.9459 87-117 0 LHAHKLRVDPVNFKLLSHCL LVTLAAHLPAE
2135.0419 32-48 0 RMFLSFPTTKTYFPHFD
1723.9238 8-24 0 KTNVKAAWGKVGAHAGE
1718.9296 49-65 0 LSHGSAQVKGHGKKVAD
1699.9741 128-142 0 KFLASVSTVLTSKYR
1057.5312 118-127 0 FTPAVHASLD
1018.4873 77-86 0 MPNALSALSD
1010.5265 66-75 0 ALTNAVAHVD
601.3191 2-7 0 VLSPAD

94.3% of sequence covered (you may modify the input parameters to display also peptides < 500
Da or > 100000000000 Da):
10 20 30 40 50 60
VLSPADKTN VKAAWGKVGA HAGEygaeal eRMFLSFPTT KTYFPHFDLS HGSAQVKGHG

70 80 90 100 110 120


KKVADALTNA VAHVDdMPNA LSALSDLHAH KLRVDPVNFK LLSHCLLVTL AAHLPAEFTP

130 140
AVHASLDKFL ASVSTVLTSK YR

Display the list of masses in raw text format to be exported into an external application

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https://web.expasy.org/cgi-bin/peptide_mass/peptide-mass.pl 1/1

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