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I.

HASIL PENGAMATAN DAN PERHITUNGAN


A. HASIL PENGAMATAN
1. Kurva kalibrasi standar baku paracetamol
Konsentrasi (ppm) absorbansi
Blanko Etanol 0
Standar 1 1 0,197
Standar 2 2 0,382
Standar 3 3 0,394
Standar 4 4 0,745
Standar 5 5 0,747

2. Bobot penimbangan sampel = 10 mg

3. Faktor pengenceran = 1000 ppm

4. Serapan sampel

Replikasi Absorbans
1 0,816
2 0,817
3 0,817

B. PERHITUNGAN
 Pembuatan Larutan Standar
Konsentrasi (ppm) larutan parasetamol 10 mg dalam 10 ml adalah :

10 𝑚𝑔
Parasetamol :
10 𝑚𝐿

10 𝑚𝑔
=
0,01 𝐿

10 𝑚𝑔
=
10 𝑚𝐿
= 100 mg/L
= 1000 μg/mL
= 1000 ppm

1. Pembuatan larutan Parasetamol 100 ppm


M1 x V1 = M2 x V2

1000 µg/mL x V1 = 100 µg/mL x 10 mL

1000 μg
V1 =
1000 μg/mL

V1 = 1 mL

2. Pembuatan larutan stok parasetamol 10 ppm


M1 x V1 = M2 x V2

100 µg/mL x V1 = 10 µg/mL x 25 mL

250 μg
V1 =
100 μg/mL

V1 = 2,5 mL

3. Perhitungan larutan standar parasetamol 1 ppm


M1 x V1 = M2 x V2

10 µg/mL x V1 = 1 µg/mL x 10 mL

10 μg
V1 =
10 μg/mL

V1 = 1 mL

4. Perhitungan larutan standar parasetamol 2 ppm


M1 x V1 = M2 x V2

10 µg/mL x V1 = 2 µg/mL x 10 mL

20 μg
V1 =
10 μg/mL

V1 = 2 mL
5. Perhitungan larutan standar parasetamol 3 ppm
M1 x V1 = M2 x V2

10 µg/mL x V1 = 3 µg/mL x 10 mL

30 μg
V1 =
10 μg/mL

V1 = 3 mL

6. Perhitungan larutan standar parasetamol 4 ppm


M1 x V1 = M2 x V2

10 µg/mL x V1 = 4 µg/mL x 10 mL

40 μg
V1 =
10 μg/mL

V1 = 4 mL

7. Perhitungan larutan standar parasetamol 5 ppm


M1 x V1 = M2 x V2

10 µg/mL x V1 = 5 µg/mL x 10 mL

50 μg
V1 =
10 μg/mL

V1 = 5 mL

 Perhitungan kurva kalibrasi

X Y X2 Y2 XY
(µg/mL) (absorbansi)
1 0,197 1 0,038809 0,197
2 0,382 4 0,145924 0,764
3 0,394 9 0,155236 1,182
4 0,745 16 0,555025 2,98
5 0,747 25 0,558009 3,735
Ʃ = 15 Ʃ = 2,465 Ʃ = 55 Ʃ =1,453003 Ʃ = 8,858

Persamaan regresi linier yaitu : y = a + bx


∑ 𝑥 2 ∑ 𝑦 − ∑ 𝑥 ∑ 𝑥𝑦
𝑎=
𝑛 ∑ 𝑥 2 − (∑ 𝑥)2

55 . 2,465 − 15 . 8,858
=
5 . 55 − (15)2

135,575 − 132,87
=
275 − 225

2,705
=
50

= 0,0541

𝑛 ∑ 𝑥𝑦 − ∑ 𝑥 ∑ 𝑦
𝑏=
𝑛 ∑ 𝑥 2 − (∑ 𝑥)2

(5 × 8,858) − (15 × 2,465)


=
5 . 55 − (15)2

44,29 − 36,975
=
275 − 225

7,315
=
50

= 0,1463

𝑛 ∑ 𝑥𝑦 − ∑ 𝑥 ∑ 𝑦
𝑟2 =
√(𝑛 ∑ 𝑥 2 − (∑ 𝑥)2 )(𝑛 ∑ 𝑦 2 − (∑ 𝑦)2 )

(5 × 8,858) − (15 × 2,465)


=
√(5(55) − (15)2 ) ((5)1,453003 − (2,465)2 )

44,29 − 36,975
=
√(275 − 225)(7,265015 − 6,076225)
7,315
=
√(50)(1,18879)

7,315
=
√59,4395

7,315
=
7,70970168

= 0,9488045457

Dari perhitungan diatas didapat persamaan garis linier dari larutan standar paracetamol

dalam pelarut etanol yaitu y = 0,0541 + 0,1463x dengan koefisien korelasinya ( r2 )

= 0,9488045457

0.9
y = 0,0313 + 0,1011x
0.8 r2 = 0.9788482113
absorbansi

0.7
0.6
0.5
0.4
0.3
0.2
0.1
0
0 1 2 3 4 5 6
Konsentrasi (µg/mL)
(µg/Ml)standar
Kurva kalibrasi (µg/mL)
parasetamol
GANGERTI BUAT YANG INI!!
 Perhitungan batas deteksi (LOD) dan batas kuantitasi (LOQ)

Persamaan garis regresi linier y = 0,0541 + 0,1463x

X Y yˈ y - yˈ (y - yˈ)2
y = 0,0541 + 0,1463𝑥
1 0,197 0,2004 -0,0034 1,156 x 10-5
2 0,382 0,3467 0,0353 1,24609 x 10-3
3 0,394 0,493 -0,099 9,801 x 10-3
4 0,745 0,6393 0,1057
5 0,747 0,7856 -0,0386

∑(𝑦 − 𝑦′)2
𝑆𝑏 = √
(𝑛 − 2)

4,4651 x 10−3
= √
(5 − 2)

= √0,001488366667
= 0,03857935545

𝑆𝑏
LOD = 3 ×
𝑏
0,03857935545
=3×
0,1011
1,144787996 µg/ml

𝑆𝑏
LOQ = 10 × 𝑏

0,03857935545
= 10 × 0,1011

= 3,815959985 µg/mL