A.

Title of Experiment : Vitamin C Analysis in Watermelon

B. Date and Time of Experiment : Wednesday, 10th October 2018 at 9.30 a.m –
12.00
C. Purpose of Experiment : To Determine The Vitamin C Level in
Watermelon
D. Basic Theories
E. Tools and Materials
1. Tools
a) Mortal and pestle 1 set
b) Graduated cylinder 10 ml; 25 ml 1 pc; 1pc
c) Pipettes 5 pcs
d) Volumetric flask 1 pc
e) Beaker glass 2 pcs
f) Burette 1 pc
g) Funnel 1 pc
h) Filtered paper 1 pc
i) Spatula 1 pc
j) Analytical balance 1 pc
k) Erlenmeyer 3 pcs
2. Materials
a) Aquades Sufficiently
b) I2 solution Sufficiently
c) Watermelon 10 grams
d) Amylum 20 drops

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F. Procedures
a) Titration in blank solution

20 ml aquades

 Poured into Erlenmeyer flask

 Added with 5 drops of 1 % strarch
 Titrated with standard I2 0,01N

Volume I2

b) Titration in the sample of watermelon

10 grams watermelon

 Peeled and weighed

 Crushed with mortal and pestle until get slurry
 Put into 100 ml volumetric flask
 Added aquades until boundary line
 Waited 15 minutes while sometimes being shaken slowly
 Filtered with filtered paper

Filtrate Residue

 Taken filtrate 10 ml
 Put into erlenmeyer flask
 Added 5 drops of 1 % starch
 Added 20 ml of aquades
 Titrated with standard iodine 0,01 N solution
 If the volume of iodine more than 2 ml, dilution 10x again
 Repeat 3 x
Volume I2

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Reaction :

(aq)+
nI2(aq) →

(aq)

(aq) + n I2(aq) → (aq) + 2HI(aq

Reduction : I2(aq) + 2e →2I- (aq)

Oxidation : C6H6O6(aq) →C6H6O6(aq) + 2e + 2H(aq)

Overall reaction : I2(aq) + C6H6O6(aq) → C6H6O6(aq) + 2HI(aq)

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 G. Results of Experiment
No. Procedures Observation Result Hypothesis/Reaction Conclusion
Before After
1. Titration of the blank

20 ml aquades

 Poured into Erlenmeyer flask

 Added with 5 drops of 1 % strarch
 Titrated with standard I2 0,01N

Volume I2 (aq)+ nI2(aq) →

(aq)

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2. Titration the sample (watermelon)
10 grams watermelon
 Peeled and weighed
 Crushed with mortal and pestle until get
slurry
 Put into 100 ml volumetric flask
 Added aquades until boundary line
 Waited 15 minutes while sometimes being (aq)+ nI2(aq) →
shaken slowly
 Filtered with filtered paper

Filtrate Residue

 Taken filtrate 10 ml
 Put into erlenmeyer flask
 Added 5 drops of 1 % starch (aq)
 Added 20 ml of aquades
 Titrated with standard iodine 0,01 N solution
 If the volume of iodine more than 2 ml, dilution
10x again
 Repeat 3 x
Volume I2

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 (aq) + n I2(aq) →

(aq) + 2HI(aq)

Reduction : I2(aq) + 2e →2I- (aq)

Oxidation :C6H6O6(aq)
→C6H6O6(aq) + 2e + 2H(aq)

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Overall reaction : I2(aq) + C6H6O6(aq)
→ C6H6O6(aq) + 2HI(aq)

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H. Analysis and Explanation
I. Discussions

J. Conclusion
K. References

L. Answer of Questions

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M. Attachments
1. Picture Attachment
Pictures Explanation
The tools which used in this experiment
are beaker glass, graduated cylinder,
volumetric flask, thermometer,
volumetric pipette, pipettes, test tubes,
test tube shelf, hotplate,
spectrophotometer.

Saliva which used as enzyme amylase

source. This solution has colourless
colour.

Dilute the saliva 10x with 1 ml saliva

added until 10 ml aquades in volumetric
flask.

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Added aquades to dilute the saliva,
aquades is colourless.

1 ml starch is colourless solution.

Added by aquades become colourless

solution.

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Added by pH solution, pH solution
which used is 1,3,5,7,9, solution for
sample solution, but for blanco not added
by pH solution as comparison. It’s
colourless solution.

Heated in 37⁰C for 5 minutes. It’s for to

conducted the system in optimum
temperature of amylase enzyme.

After heated, the solution still colourless.

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After added by two drops of iodine, the
blanco solution changes to dark blue
solution, the pH1 changes in to dark blue
solution, pH 3 solution changes in to
light yellow solution, pH 5 changes to
light purple, pH 7 changes to yellow
solution, and for pH 9 changes into
purple solution.

Added by 6 ml aquades to decrease the

concentration of sample and blanco. The
all colour of sample and blanco become
lighter than before.

Checking the absorbance of sample and

blanco with spectrophotometer with
wavelength 680 nm which specific for
complex iod-amylum.

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Dilution process for 10x, 100x, 1000x,
10000x, and 100000x saliva.

Various concentration of saliva which

added by aquades was colourless. Also
after added with pH 3 solution as pH
optimum in its experiment, the solution
was colourless.

Added by amylum, the sample and

blanco were colourless.

Heated in 37⁰C as optimum

themperature of amylase activity.

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Heated in 70⁰C to stop the activity of
amylase activity.

after heating process the solution still

colourless.

After added with 2 drops of iodine, the

blanco solution changes to dark blue, the
10x dilution saliva changes to light
yellow, the 100x dilution saliva changes
to blue solution, the 1000x dilution
saliva changes to blue++ solution, the
10000x dilution changes to dark blue
solution, and for the 100000x dilution
changes to dark blue solution.

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After added with 6 ml aquades, the
colour changes to lighter than before.

Testing the absorbance of sample and

blanco which will shown the relation
between enzyme concentration with its
activity.

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