HASIL UJI FMD

Uji Normalitas data:

Kolmogorov-Smirnova Shapiro-Wilk

waktu perlakuan Statistic df Sig. Statistic df Sig.

FMD Vit C PRA_PERLAKUAN .203 16 0.077 .819 16 0.005

PASCA PERLAKUAN .164 16 0.200* .863 16 0.021

FMD Plasebo PRA_PERLAKUAN .373 16 0.000 .631 16 0.000

PASCA PERLAKUAN .366 16 0.000 .654 16 0.000

a. Lilliefors Significance Correction

*. This is a lower bound of the true significance.

Hasil : data tidak normal  uji alternatifnya: Wilcoxon

Test Statisticsb

waktu perlakuan waktu perlakuan

- FMD Vit C - FMD Plasebo

Z -4.937a -4.937a

Asymp. Sig. (2-tailed) 0.000 0.000

a. Based on negative ranks.

b. Wilcoxon Signed Ranks Test

Kesimpulan: terdapat perbedaan FMD Pasca dan Pra perlakuan yang bermakna (p <0,001), baik kelompok vit C maupun placebo.
Selisih FMD Kelompok Vit C vs Plasebo
Tests of Normality

Kolmogorov-Smirnova Shapiro-Wilk

Kelompok Statistic df Sig. Statistic df Sig.

Selisih FMD Pasca-pra Vita C .121 16 0.200* .931 16 0.250

perlakuan
Plasebo .258 16 0.006 .883 16 0.043

a. Lilliefors Significance Correction

*. This is a lower bound of the true significance.

Group Statistics
Kelompok N Mean±Std. Deviation
Selisih FMD Pasca-pra Vita C 16 0.023±0.035
p=0,003
perlakuan Plasebo 16 -0.04±0.068

Kesimpulan: Vit C lebih meningkatkan FMD secara bermakna (p=0,003) dibandingkan placebo.
 NUCLEIC ACID OXIDATION 8-HIDROXY-2-DEOXYGUANOSINE (8-OHDG)
AND INCREASED OF FLOW-MEDIATED DILATATION (FMD) IN
NON DIABETIC CHRONIC KIDNEY DISEASE (CKD)
PATIENTS WITH HAEMODIALYSIS

Musrifah Budi Utami *, Diding HP**, Widiastuti ***, Bambang Purwanto****

* Resident of Internal Medicine Faculty of Medicine Sebelas Maret University-Dr.Moewardi Hospital Surakarta
** Immunology Division Biomedic Lab Sebelas Maret University, Surakarta
***Departement Radiology Dr Moewardi Hospital Surakarta
**** Nephrology Division, Internal Medicine Faculty of Medicine Sebelas Maret University-Dr.Moewardi Hospital Surakarta

ABSTRACT

Background : The most common cause of mortality in patients with CKD stage V is cardiovascular event (60%). Cardiovascular
event is mainly constituted by atherosclerosis. One of the underlying mechanism of atherosclerosis is endothelial dysfunction.

Objectives : To evaluate the effect of vitamin C in the improvement of endothelial dysfunction which characterized by decreased
levels of nucleic acid oxidation 8-OHDG and increased FMD.

Methods : In this randomized, double blind, placebo-controlled trial, 32 non diabetic chronic kidney disease patients were recruited,
aged 18-59 years with twice a week hemodialysis within 3 months - 5 years. 16 patients as control then other 16 patiens had vitamin
C 10 mg/kg/day for 4 weeks. Nucleic acid oxidation 8-OHDG levels was measured by enzyme-linked immunoabsorbent assay
(ELISA) kit and flow-mediated dilation (FMD) of brachial artery by doppler ultrasonography before and after treatment.

Results : Nucleic acid oxidation 8-OHDG levels reduced significantly in CKD stage V patients with hemodialysis after vitamin C
administration (vitamin C vs placebo : -2.481.90 vs 0.583.05, p<0.001) and FMD increased significantly (vitamin C vs placebo:
0.023±0.035vs-0.04±0.068 , p=0.003)

Conclusion: Vitamin C can improve endothelial dysfunction which characterized by decreased levels of nucleic acid oxidation 8-
OHDG and increased FMD significantly.

Keywords : Vitamin C, Nucleic Acid Oxidation 8-OHDG, Flow-Mediated Dilatation, Non Diabetic CKD Stage V
 8OHDG
1
0.58
0.5

0
Vit C Plasebo
-0.5

-1

-1.5

-2

-2.5
-2.48
-3