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◎ About Probiotics
What are probiotics
Wh
CONTENTS There are more than 1,000 different species of bacteria, fungi, and viruses living in the human body, comprising over 100 trillion
CFU. T
These microbes, friendly and pathogenic, form a delicate and dynamic balance as they compete to colonize and survive in our
bodies Most microbes in the human body commonly reside in the mouth, pharynx, respiratory system, gastro-intestinal tract,
bodies.
urogen
urogenital tract, and skin. Our gastro-intestinal tract is the biggest and most complicated ecosystem for the microbes in general and
bacter in particular.
bacteria
These microbes participate in the host body’s metabolism by producing metabolic ingredients such as organic acids (especially
a
lactic acid and acetic acid); synthesizing vitamins, enzymes, and antibodies; and regulating the metabolism and synthesis of sugar,
an proteins. The host body’s health for a large part is related with the resident microbes’ health and composition.
fat, and
When the balance of the friendly microbes and pathogenic microbes is disturbed, the host body becomes prone to diseases or other
About Probiotics health challenges. Having an optimal number of friendly microbes (probiotics) colonizing our gastro-intestinal tract helps us prevent
diseas and is good for our health.
diseases
1. What are probiotics
2. Categories and health benefits of probiotics
3. Applications of probiotic lactic acid bacteria
4. Criteria for selecting beneficial probiotics
01
◎ About Probiotics ◎ About Probiotics
Categories and health benefits of probiotics Applications of probiotic lactic acid bacteria
App
■ Lactic Acid Bacteria include Lactobacillus species, Bifidobacterium species, Coccus species, etc.
■ Probiotic yeasts and fungi Categories
Ca Industry Domains Applications
■ Bacillus
Probiotic drugs
Water
☆ Neurological effects Neurological effects treatment
02 03
◎ About Probiotics ◎ About BioGrowing
Manufacturer’s Third party qualifications and supplier side audit will be helpful (5) Probiotic
Pro food programs, such as bakery, drinks, snacks, etc.
qualifications to monitor the quality system and potential quality risks. (6) Dairy
Da starter cultures (for yoghurt, yoghurt drink, sour cream, cottage cheese, soft cheese, etc.)
Popular lactic acid probiotic bacteria have been used for a very (7) Homemade
Ho products, such as homemade yoghurt cultures, homemade yoghurt powder, homemade pickled vegetables, etc.
With increasing research in the probiotics domain, SRAs (Strict Regulatory Authorities) across the globe tend to approve probiotics
as safe food ingredients. Most large probiotic companies have established robust quality and safety protocols to support their
business.
04 05
◎ About BioGrowing ◎ About BioGrowing
BioGrow ing’ s business and a state-of-art facility BioGrow ing’ s approach to making quality probiotic products
Bio
■ Owns China’s largest production line for Lactic Acid Bacteria with an annual capacity of manufacturing 300 tons of high potency With a 100,000 class GMP facility, we meet the requirements for medication production.
probiotic powder
■ Production lines are certified to meet the criteria set by CFDA-GMP/QS, ISO/HACCP, NSF-CGMP, Halal, etc.
■ Deploy patented industry-leading fermentation technology
■ Recognized as a high-tech enterprise by the Shanghai municipal government
■ Participated in the drafting of regulations for the probiotic industry in China
1) “Approved probiotic species for human foods” (2010, Ministry of Health, China)
2) “Lactic Acid Bacteria for food process” Industry guideline (2014, Ministry of Health, China)
3) “National Standards for food safety: General requirements for bacteria species for food” (2014, Ministry of Health, China)
BioGrow ing state-of-art production facility Manufacturing 1) Production in GMP factory (class 100,000 clean
area)
process 2) With patented fermentation technology,
cultivating an advanced level of highly-active
bacteria amount with high potency
3) With patent protected technology ensuring
excellent stability
4) Strict production management ensuring
excellent product quality
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◎ About BioGrowing Products ◎ About BioGrowing Products
Flora-Focus® bulk probiotic ingredients Typical strain’ s quality indicators and stability
Typ
Following is a list of our probiotic strain with specifications:
Items Specification Bifidobacterium lactis BL-G101 shelf stability at -18°C
BioGrow ing Code Strains Generic Name Chinese Name 中⽂文名 Potency(CFU/g)
1. Ap
Appearance Off-white or light yellow
LA-G80 Lactobacillus acidophilus 嗜酸乳杆菌 2.0*1011
powder
Lp-G18 Lactobacillus plantarum 植物乳杆菌 5.0*1011
2. Ge
Genetic Identification 16s-rRNA method etc.
LR-G100 Lactobacillus reuteri 罗伊⽒氏乳杆菌 2.0*1011 3. IDA deposit number CCTCC M 2013336
Lr-G14 Lactobacillus rhamnosus ⿏鼠李李糖乳杆菌 5.0*1011 4. Liv
Live Bifidobacterium ≥ 5.0*1011 CFU/g
LC-G11 Lactobacillus casei ⼲干酪酪乳杆菌 5.0*10 11
lactis potency
LPc-G110 Lactobacillus paracasei 副⼲干酪酪乳杆菌 4.0*10 11
5. Water
Wa content ≤ 5.0%
LB-G40 Lactobacillus delbrueckii subsp. bulgaricus 保加利利亚乳杆菌 5.0*1010 6. Non
No lactic acid bacteria ≤ 500 CFU/g
LL-G41 Lactobacillus delbrueckii subsp.lactis 德⽒氏乳杆菌乳亚种 5.0*10 10 (1) C
Coliforms ≤ 10 CFU/g
LS-G60 Lactobacillus salivarius 唾液乳杆菌 1.0*10 11 (2) Bacillus
B ≤ 50 CFU/g
08 09
◎ About BioGrowing Products ◎ About Products
Probiotic yoghurt starter culture
Prob
Typical strain’ s quality indicators and stability BioGrowing homemade probiotic yoghurt starter culture is 100% natural.
BioGrow
10 11
◎ About Products ◎ About Products
Specification: 225 grams/bag Specification: 225 grams/bag Specification: 225 grams/bag Specifi
Specification: 225 grams/bag Specification: 225 grams/bag Specification: 225 grams/bag
One bag prepares 1000 ml of probiotic yoghurt One bag prepares 1000 ml of probiotic yoghurt One bag prepares 1000 ml of probiotic yoghurt ba prepares 1000 ml of probiotic yoghurt
One bag One bag prepares 1000 ml of probiotic yoghurt One bag prepares 1000 ml of probiotic yoghurt
Storage: In a cool and dry place Storage: In a cool and dry place Storage: In a cool and dry place Storag
Storage: In a cool and dry place Storage: In a cool and dry place Storage: In a cool and dry place
Shelf life: 18 months Shelf life: 18 months Shelf life: 18 months Shelf life:
li 18 months Shelf life: 18 months Shelf life: 18 months
Item Per 100 g NRV% Item Per 100 g NRV% Item Per 100 g NRV% Item Per 100 g NRV% Item Per 100 g NRV% Item Per 100 g NRV%
Energy 1921 KJ 23% Energy 1914 KJ 23% Energy 1921 KJ 23% Energy 1936 KJ 23% Energy 1947 KJ 23% Energy 1962 KJ 23%
Protein 15.0 g 25% Protein 14.7 g 25% Protein 15.0 g 25% Protein 16.2 g 27% Protein 16.2 g 27% Protein 15.8 g 26%
Fat 17.4 g 29% Fat 17.0 g 28% Fat 17.4 g 29% Fat 18.7 g 31% Fat 18.8 g 31% Fat 20.4 g 34%
Carbohydrate 60.1 g 20% Carbohydrate 60.9 g 20% Carbohydrate 60.1 g 20% Car
Carbohydrate 57.0 g 19% Carbohydrate 57.4 g 19% Carbohydrate 55.2 g 18%
Na 284 mg 14% Na 278 mg 14% Na 284 mg 14% Na 305 mg 15% Na 306 mg 15% Na 298 mg 15%
Ca 464 mg 58% Ca 454 mg 57% Ca 464 mg 58% Ca 499 mg 62% Ca 501 mg 63% Ca 487 mg 61%
12 13
◎ About Products ◎ About Products
【The Shining Essence】Healthcare Probiotics Products 【BIOTIC HEALTH】Probiotic Supplements and Services
【BI
Backe by our scientific research and understanding of the market, BioGrowing is dedicated to provide comprehensive probiotic health
Backed
SHINING ESSENCE CAPSULE solutions to our partners. Our Biotic Health™ services are as follows:
solutio
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◎ About Products ◎ About Products
Glass bottle with desiccant ★★ Resists humidity to a good extent At least 10 Billion CFU live probiotic bacteria per capsule at the time of manufacturing,
bacteria amount may vary in the shelf storage condition.
Resists oxidation to a good extent Dairy free, Gluten free, Soy Free
15 selected
selecte Flora-Focus® Probiotic strains
Total carbohydrate 0.8g <1% STORAGE:
Flora-Focus® Probiotic blend - 15 strains of 20 Billion CFU+ 1.0g **
Inadequate suppleness Flora-Focus® Probiotic:
F
In a cool and dry place, preferably frozen.
Avoid high temperatures.
Plastic strips ★ Not recommended Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus casei,
Lactobacillus paracasei, Lactobacillus reuteri, Lactobacillus rhamnosus, SHELF LIFE:
Paper-plastic strips ★ Not recommended Lactobacillus salivarius, Lactobacillus helveticus, Lactobacillus bulgaricus,
Lactobacillus gasseri, Bifidobacterium lactis, Bifidobacterium bifidum,
24 months
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◎ About Products ◎ About Products
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◎ About Products ◎ About Products
【Biot
Biotic Health】 SUPPLEMENT FACTS FEATURES:
【Biotic Health】 SUPPLEMENT FACTS FEATURES: Classic combination of probiotics and prebiotics with multi-minerals and
- Helps the body to get in shape 乐+ 活性
活性益生菌粉 Serving Size: 1 sachet, 2.0g per sachet Serving Per Box:30
女性产后恢复调理的配方 multi-vitamins supplements.
Serving Size: 1 sachet, 3.0g per sachet Serving Per Box:30 - Hormone regulation (Joy+ L
Live Probiotic Powder)
(Postpatrum + Live Probiotic Powder) - Promote lactation Nutrition Index Amount Per 100g NRV%
Nutrition Index Amount Per 100g NRV% Classic combination of live probiotic bacteria with a potency of 50 billion CFU Energy 850kJ 10% DIRECTIONS:
Energy 1638kJ 20% per sachet, lactitol, and fish collagen peptide to promote postpartum recovery. Protein 1.0g 2% For anyone over 1 year of age, 1 serving per day with milk formula or food.
Protein 15.8g 26% Fat 0g 0% Consult a physician in case of an existing medical condition. Keep it out
DIRECTIONS: Carbohydrate 24.1g 8%
Fat 0g 0% For adults, one serving each time. Dissolve in water with temperature not of the reach of children.
Dietary fiber 61.8g 25%
Carbohydrate 80.6g 27% more than 40℃. Consult a physician in case of an existing medical
Sodium 187mg 9%
Sodium 144mg 7% condition. Keep it out of the reach of children.
Vitamin A 1400μgRE 175%
STORAGE:
Flora-Focus® Probiotic Blend - 8 strains of 50 billion CFU* 3.0g In a cool and dry place, preferably frozen.
STORAGE: Vitamin D 1.5μg 30%
Avoid high temperatures.
In a cool and dry place, preferably frozen. Vitamin B1 1.50mg 107%
Lactobacillus acidophilus, Bifidobacterium lactis, Lactobacillus rhamnosus, Lactobacillus Avoid high temperatures. Vitamin B2 1.50mg 107%
plantarum, Lactobacillus johnsonii, Lactobacillus crispatus, Bifidobacterium longum. Vitamin C 150.0mg 150% SHELF LIFE:
Lactitol, Water soluble fiber, FOS(Oligosaccharide fructose), Fish collagen SHELF LIFE: Calcium 540mg 68% 24 months
peptide,XOS(Xylooligosaccharide). 24 months Iron 14.0mg 93%
Zinc 14.00mg 93%
Stated health benefits of this product have not been evaluated by the Food and Drug Administration
Authority. This product is not intended to diagnose, treat, cure, or prevent any disease. Flora-Focus® Probiotic Blend - 3 strains of 10 billion CFU* 2.0 g
Bifidobacterium lactis, Lactobacillus reuteri, Lactobacillus rhamnosus,
Water soluble fiber, FOS (fructo-oligosaccharide), Orange fruit powder, Composite
minerals (L-calcium lactate, Ferrous lactate, Zinc gluconate), Stated health benefits of this product have not been evaluated by the Food and Drug Administration
Composite vitamins (Vitamin A/D/B1/B2/C) Authority. This product is not intended to diagnose, treat, cure, or prevent any disease.
【Biotic Health】 SUPPLEMENT FACTS FEATURES:
- Skin care
美+ 活性益生菌粉 Serving Size: 1 sachet, 3.0g per sachet Serving Per Box:30 - Relief from constipation
(Beauty+ Live Probiotic Powder) - Reproductive system health
Nutrition Index Amount Per 100g NRV% Classic combination of live probiotic bacteria. Each sachet has a potency of 【Biotic Health】
【Bioti SUPPLEMENT FACTS FEATURES:
Energy 1619kJ 19% 50 billion CFU and contains lactitol and dietary fiber for a better digestion. - Reduces sugar absorption
Protein 1.8g 3% 控糖吸收 益生菌粉 Serving Size: 1 sachet, 2.0g per sachet Serving Per Box:30 - Maintains blood sugar
DIRECTIONS: (Diabe-Relief Probiotic Powder)
(Diabe- - Regulates metabolic balance
Fat 0g 0%
For adults, one serving each time. Dissolve in water with temperature not Nutrition Index Amount Per 100g NRV%
Carbohydrate 93.5g 31% more than 40℃. Consult a physician in case of an existing medical condition. Energy 1643kJ 20%
Sodium 146mg 7% Keep it out of the reach of children.
Protein 0.7g 1% DIRECTIONS:
Flora-Focus® Probiotic Blend - 7 strains of 50 billion CFU* 3.0g For adults, one serving each time, with milk formula food, or water.
STORAGE: Fat 0g 0%
Take half an hour before eating. Consult a physician in case of an existing
In a cool and dry place, preferably frozen. Carbohydrate 96.6g 32% medical condition. Keep it out of the reach of children.
Bifidobacterium lactis, Lactobacillus rhamnosus, Lactobacillus acidophilus, Lactobacillus Avoid high temperatures.
plantarum, Lactobacillus johnsonii, Lactobacillus gasseri, Bifidobacterium longum. Sodium 55mg 3%
STORAGE:
Lactitol, Water soluble fiber, FOS(fructo-oligosaccharide), XOS(xylooligosaccharide), SHELF LIFE: Flora-Focus® Probiotic Blend - 4 strains of 10 billion CFU* 2.0 g
In a cool and dry place, preferably frozen.
Cranberry powder.
24 months Avoid high temperatures.
Bifidobacterium lactis, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus plantarum.
Stated health benefits of this product have not been evaluated by the Food and Drug Administration
Authority. This product is not intended to diagnose, treat, cure, or prevent any disease.
Water soluble fiber, L-arabinose, FOS(Oligosaccharide fructose), SHELF LIFE:
GOS(Oligogalactose), Orange powder.
24 months
Stated health benefits of this product have not been evaluated by the Food and Drug Administration
Authority. This product is not intended to diagnose, treat, cure, or prevent any disease.
GOS(galactooligosaccharides), Coniferous cherry powder. Stated health benefits of this product have not been evaluated by the Food and Drug Administration
Bifidobacterium longum, Bifidobacterium lactis, Lactobacillus casei, Lactobacillus reuteri, SHELF LIFE:
Authority. This product is not intended to diagnose, treat, cure, or prevent any disease.
Lactobacillus rhamnoosus, Lactobacillus acidophilus, Lactobacillus plantarum. 24 months
Water soluble fiber, Oligomeric isomaltose, Glucose, Maize oligopeptide.
Stated health benefits of this product have not been evaluated by the Food and Drug Administration
Authority. This product is not intended to diagnose, treat, cure, or prevent any disease.
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◎ About Products ◎ About Products
BioGuard ® starter cultures (for industrial use) Thermophilic dairy starter cultures
The
GF479 Character indicators
GF47
Culture’s composition Characters
Species composition
Sp GF479 Typical Characters
speed
Fermentation
Viscosity
Flavor
Thickness
Post-acidity
Product type Streptococcus thermophilus ST-G30
St ■ Fermentation speed ★★★★
ST LB LL.L LL.C LA BL LC LPc Lr LR LF Lp ■ Viscosity ★★
Lactobacillus delbrueckii subsp. bulgaricus LB-G40
La
■ Flavor ★★★★
(Freeze
(F dried starter cultures for industrial fermented dairy
■ Thickness ★★★★
Thermophilic dairy starter cultures use,
us such as set/stirred yoghurts.) ■ Post-Acidity ★★
GF 231 + + ★★★ ★★ ★★★ ★ ★★★
GF 468 + + ★★★★ ★★★ ★★ ★★ ★★★
Fig. 1
GF 479 + + ★★★ ★★ ★★★★ ★★★★ ★★
GF 655 + + ★★★ ★★ ★★★★ ★★★ ★★ Fermentation condition: 2.5% protein (whole milk).
GF 665 + + ★★★ ★★★ ★★★ ★★★★ ★★ T.S.: dissolution 55o C, homogeneity 65oC/20 MPa.
GF 669 + + ★★★ ★★★ ★★★ ★★★ ★★★
GF 686 + + ★★★★ ★★★ ★★★ ★★★ ★★★
GF 867 + + ★★★ ★★★★ ★★ ★★★★★ ★★
GF 868 + + ★★ ★★★★ ★★★ ★★★★★ ★★
GF 967 + + ★★★ ★★★★ ★★ ★★★★★ ★★
GFY 111 + + ★★ ★ ★★★★★ ★★ ★★★ Fermentation condition: 42oC, 2.9% protein (whole milk).
GFY 112 + + + ★★ ★ ★★★★ ★★★ ★★★ T.S.: dissolution 55oC, homogeneity 65oC/20MPa.
GFY 611 + + + ★★★★★ ★★★ ★★★★ ★★★★ ★★★
Storage: 2 - 6oC.
GP 101 + + + ★★ ★ ★★★★ ★★★ ★★★
Remark:
(1)Thermophilic starter culture: Culture fermentation temperature range preferred at 37oC~44oC.
(2)Mesophilic starter culture: Culture fermentation temperature range preferred at 25oC ~37oC.
(3)ST: Streptococcus thermophilus, LB: Lactobacillus delbrueckii subsp. bulgaricus; LL.L: Lactococcus lactis subsp. lactis;
LL.C: Lactococcus lactis subsp. cremoris; LA: Lactobacillus acidophilus; BL: Bifidobacterium lactis; LC: Lactobacillus casei;
LPc: Lactobacillus paracasei; Lr: Lactobacillus rhamnosus; LR: Lactobacillus reuteri; LF: Lactobacillus fermentum;
Lp: Lactobacillus plantarum.
Characters: ★- Very Low ★★ - Low; ★★★ - Medium; ★★★★ - High; ★★★★★ – Very High. * More data and production recipe can be provided upon request.
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◎ About Products ◎ About Products
Fig. 2
GFY611 Survival Rate-Acid Tolerance
pH value: 3.0 adjusted with hydrochloric acid.
Fig. 2
o
Fermentation conditions: 3.0%protein (whole milk), 37 C.
T.S.: dissolution 55oC, homogeneity 65oC/20 MPa
Storage: 2 - 6oC.
Fig. 3
Fig. 3 GFY611 Survival Rate-Bile Salt Tolerance
Bile salt: Pig bile salt (0.2% w/v) dissolve in sterilized
Fermentation conditions: 37oC, whole milk
saline.
T.S.: dissolution 55oC, homogeneity 65oC/20 MPa.
24 25
◎ About Products ◎ About Products
Probiotic dairy and beverage starter cultures Probiotic dairy and beverage starter cultures
Pro
GFY114 Character indicators GFY112 Character indicators
GFY1
Fig1.
Fig. 1
Fermentation conditions:37oC,2.5% protein(whole
Fermentation conditions: 2.5% protein (whole milk), 37oC.
milk),6.0% glucose.
T.S.: dissolution 55oC, homogeneity 65oC/20 MPa
T.S.:dissolution 55oC,homogeneity 65oC/20MPa
Fig2.
GFY114 Survival Rate-Acid Tolerance Fig. 2
pH value: 3.0 adjusted with hydrochloric acid.
pH value: 3.0 adjusted with hydrochloric acid.
Fig3. Fig. 3
GFY114 Survival Rate-Acid Tolerance Bile salt: Pig bile salt (0.2% w/v) dissolve in sterilized saline
Bile salt: Pig bile salt (0.2% w/v) dissolve in sterilized
saline.
* More data and production recipe can be provided upon request. * More data and production recipe can be provided upon request.
26 27
◎ About Products ◎ About Products
Probiotic dairy and beverage starter cultures (same category as Yakult) LactoSour - Starter culture for Sour Beer
Lac
Sour beer,
b one of the oldest styles of beer in the world, is becoming a new trend nowadays. Besides yeasts, we also use
GF201 Character indicators
Lacto
LactoSourTM Lactobacillus bacteria to produce a tasty, characteristic sourness.
Species composition GF201 Typical Characters
Ing
Ingredients: Lactobacillus acidophilus LA-G80, Lactobacillus plantarum
Lactobacillus casei LC-G11 ■ Fermentation speed ★★
Lp-G18, Lactobacillus bulgaricus LB-G40, Lactococcus lactis
Lactobacillus delbrueckii subsp. bulgaricus LB-G40 ■ Viscosity ★
subsp. Lactis LL-G41, Lactobacillus rhamnosus Lr-G14
(Freeze dried starter culture for industrial fermented dairy and ■ Flavor ★★
Fig1. Op
Optimal fermentation
37oC
o tem
temperature:
Fermentation conditions: 37 C, 4.0% protein (skim
milk), 6.0% glucose.
T.S.: dissolution 55oC, homogeneity 65oC/20 MPa,
Using direction:
Inocu
Inoculate (part of) your wort with Lactobacillus. Allow the wort to sour at the temperature recommended for the lactic acid
culture
culture. When desired sourness is achieved, boil the wort for 10 minutes to kill the lactic acid bacteria. Let the brew cool
*More data and production recipe can be provided upon down and then blend with the normally fermented beer. Allow the yeast to finish off any unused sugars from the
request. lactic-
lactic-fermented wort.
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