DNA TECHNOLOGY

Tanyaratana Dumkua
Biology Department, Mahidolwittayanusorn School
 DNA & Restriction enzyme

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 Palindrome sequence

https://pmgbiology.files.wordpress.com/2014/11/image001.gif
http://www.accessexcellence.org/RC/VL/GG/images/restriction.gif
Sticky end & Blunt end

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Restriction enzyme

http://www.eplantscience.com/index/images/Biotechnology/chapter03/017.jpg
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http://cglab.ca/~morin/teaching/compbio/f_s02gelelect.gif
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http://bio1151.nicerweb.com/Locked/media/ch20/20_08GelElectrophoresis.jpg
http://www.apsnet.org/edcenter/K-12/TeachersGuide/PlantBiotechnology/PublishingImages/act3fig02.jpg
Gel Electrophoresis and application

Comb, Gel setting, Electrophoresis chamber, Power supply

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http://2011.igem.org/wiki/images/7/73/Gel_Electrophoresis.jpg
Gel document & UV box Ethidium Bromide & UV

http://2008.igem.org/wiki/images/thumb/0/07/Ethidium2.jpg/231px-Ethidium2.jpg
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 Ethidium Bromide & UV

http://upload.wikimedia.org/wikipedia/commons/c/c8/Ethidium-bromide-abs.png
 Move by Size
http://www.nslc.wustl.edu/courses/bio2960/labs/07DNA/Gel/f21.gif
http://www.phschool.com/science/biology_place/labbench/lab6/images/overview.gif
 Why do DNA move as
this direction?

Agarose gel or Acrylamide gel

http://upload.wikimedia.org/wikipedia/commons/e/e9/Gel_electrophoresis_procedure.png
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http://www.mhhe.com/biosci/esp/2001_gbio/folder_structure/ge/m6/s2/assets/images/gem6s2_2.jpg
 Southern Blot

http://employees.csbsju.edu/hjakubowski/classes/ch331/dna/southernblot.jpg
 Restriction Fragment Length Polymorphisms
http://www.ncbi.nlm.nih.gov/core/assets/probe/images/rflp_genotyping.gif
http://www.discoveryandinnovation.com/BIOL202/notes/images/RFLP_map.jpg
 DNA Finger Printing

http://www.mob.com.pk/images/forensic2.jpg
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https://www.mun.ca/biology/scarr/MGA2-9-06smc.jpg
http://upload.wikimedia.org/wikipedia/commons/7/7a/Codis_profile.jpg
 Soldier’s parents

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http://evolution.berkeley.edu/evolibrary/images/news/dnafingerprints.gif
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 Polymerase
Chain
Reaction

https://www.neb.com/~/media/NebUs/Page%20Images/Applications/DNA%20Amplification%20and%20PCR/pcr.jpg
 Clip เรื่อง PCR (ความยาว 3.55 นาที)

https://www.youtube.com/watch?v=iQsu3Kz9NYo
 RAPD : random amplification of polymorphic

http://openi.nlm.nih.gov/imgs/512/301/2885095/2885095_ijms-11-02079f4.png
จงเปรียบเทียบการจาลองตัวเองแบบ

In vivo replication & In vitro replication

 Genetic Engineering

1. Prepare target gene

2. Prepare vector
3. Recombinant DNA
4. Transfer recombinant
gene to target
5. Clone or induce to
be GMOs

http://media-1.web.britannica.com/eb-media/44/117844-004-46C4F162.jpg
 Clip เรื่อง Steps in Recombinant DNA technology or rDNA
technology ความยาว 8.17

https://www.youtube.com/watch?v=ZW9zPdb_Bs0
 Multiple cloning site (MCS) / Polylinker

http://faculty.plattsburgh.edu/donald.slish/figures/Bluescript.GIF
http://bioserv.fiu.edu/~biolab/labs/Genetics/digestion_files/image008.gif
Cloning vector Expression vector

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 Lac operon

https://www.youtube.com/watch?v=h5p05aFzWdA
https://www.youtube.com/watch?v=2sMFswbOgKk

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 IPTG & X-gal

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 Recombinant DNA

https://www.youtube.com/watch?v=juP6iHMIYkE
http://www.bio.miami.edu/dana/pix/chimericDNA.gif
 Transformation

http://www.odec.ca/projects/2006/sidh6h2/images/bgdiagram.gif
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 Agrobacterium
&
Ti plasmid

http://biology-forums.com/index.php?action=gallery;sa=view;id=599
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 CRISPR
Genetic Engineering Will Change Everything
Forever (16.03 min)

https://www.youtube.com/watch?v=jAhjPd4uNFY