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Journal of the Saudi Society of Agricultural Sciences (2017) 16, 89–96

King Saud University

Journal of the Saudi Society of Agricultural Sciences


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FULL LENGTH ARTICLE

The nutritional composition of fruit jams


in the Malaysian market
M.N. Mohd Naeem a, M.N. Mohd Fairulnizal a,*, M.K. Norhayati a, A. Zaiton b,
A.H. Norliza b, W.Z. Wan Syuriahti b, J. Mohd Azerulazree a, A.R. Aswir a,
S. Rusidah c

a
Nutrition Unit, Cardiovascular, Diabetes and Nutrition Research Centre, Institute for Medical Research, Jalan Pahang, 50588
Kuala Lumpur, Malaysia
b
Food Section, Chemistry Department, Environmental Health Division, Chemistry Department, Jalan Sultan, 46661 Petaling
Jaya, Selangor, Malaysia
c
Food Division, Aras 1, Blok E3, Parcel E, Pusat Pentadbiran Kerajaan Persekutuan, 62590 Putrajaya, Malaysia

Received 13 January 2015; revised 24 February 2015; accepted 2 March 2015


Available online 14 March 2015

KEYWORDS Abstract Fruit jams are preserved fruits and sugars normally canned or sealed for long-term stor-
Nutritional; age. Jam making involves the disruption of the fruit tissue followed by heating with added water
Jams; and sugar to activate its pectin before being put into containers. Processes that expose foods to high
Grape; levels of heat may cause some nutrient loss. Hence, the objective of this study was to evaluate the
Apricot; nutritional composition of four commonly consumed fruit jams that are available in the Malaysian
Blueberry; market. Different brands (n = 6) of each type of fruit jams (grape, apricot, blueberry and straw-
Strawberry berry) were sampled from supermarkets in Klang Valley, Malaysia. The sampling method used
was stratified random sampling. The fruit jams were analyzed for the presence of 27 important
nutrients using Association of Official Agricultural Chemists (AOAC) official methods of analysis.
This study showed that fruit jams are a good source of energy and carbohydrate. The fruits jams
have very low levels of fatty acids. Fruit jams may provide an affordable and convenient source
of energy and carbohydrate. The data can be utilized to contribute to the enhancement of
Malaysia Food Composition Database.
ª 2015 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University. This is
an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

* Corresponding author. Tel.: +60 3 26162638; fax: +60 3


26939335. 1. Introduction
E-mail address: fairul@imr.gov.my (M.N. Mohd Fairulnizal).
Peer review under responsibility of King Saud University. Several types of fruits have been reutilized in the production
of value added food products such as jams. Jams are pro-
duced by the preservation of fruits which are canned or
sealed to extend their shelf lives. Among American English,
Production and hosting by Elsevier
jams are considered to be a type of food preserve.
http://dx.doi.org/10.1016/j.jssas.2015.03.002
1658-077X ª 2015 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University.
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
90 M.N. Mohd Naeem et al.

However, among the British and Commonwealth English the 2.2. Proximate analysis
term ‘‘jams’’ often refer to all types of food preserves.
Normally, the jam preparations require the addition of com- The energy content of each type of jams was calculated by
mercial or natural pectin as a gelling agent (Madhav and summation of multiplication of protein, carbohydrate and
Pushpalatha, 2002). The usage of ingredients and how the fat by values of 4, 4 and 9 factors, respectively. The value is
jams are prepared actually determine the type of preserves; expressed as kilocalories (kcal). The moisture content was
jams, jellies and marmalades. determined by drying of finely ground samples (10 g) in air-
Jams are one of the most popular food products because of oven at 105 C overnight until a constant weight was achieved
their low cost, all year long availability and organoleptic prop- (AOAC, 2008). Protein content was determined based on
erties (Gakowska et al., 2010). Traditionally, jams were first Kjeldahl method (AOAC, 2005). A conversion factor of 6.25
produced as an effort to preserve fruit during off-season for was used to convert the measured nitrogen content to protein
consumption. Jams can be defined as a product with a total content.
soluble solid content of 45 Brix and consist of at least 40% Fat content was determined by using a semicontinuous sol-
fruit content. vent extraction method (AOAC, 2006). Briefly, finely ground
In jams manufacturing, the fruits and sugar are mixed in sample (1–3 g) was weighed into a hydrolyzing capsule for
similar proportions. The mixed product is then cooked to pro- hydrolysis. The hydrolysis was done using an automated
duce a delicious substance that possesses sufficient storage hydrolysing unit (Gerhardt, Germany). Fat was extracted
capabilities. Using extreme thermal treatment, the mix is con- using petroleum ether between 40 C and 60 C using auto-
centrated to acquire the necessary final total soluble solid con- mated fat extraction system (Gerhardt, Germany). The extract
tent (Igual et al., 2013). The downside to this process is that it was then dried for 3 h. The product was then cooled in a stan-
imparts unsavoury colour, flavour and nutritional values to dard desiccator (desiccant = silica gel beads) for 1 h before
the product. This is attributed to the extreme heat generated being weighed to measure the fat content.
and lengthy duration of processing. There is a method that The carbohydrate content was calculated by subtracting the
involves the usage of microwave energy that provides a faster sum of protein, fat, moisture, ash and total dietary fibres
heating procedure (Igual et al., 2010). Overall, by using other (TDF) from 100% (Menezes et al., 2004). TDF were deter-
methods aside from conventional means, processing time can mined using an enzymatic–gravimetric method (AOAC,
be shortened which may contribute to a better and more desir- 2005). Briefly, finely ground sample (1 g) was subjected to a
able product. sequential enzymatic digestion. For estimation of TDF, the
Nevertheless, processed products such as jams tend to have enzyme digestate was treated with ethanol to precipitate the
lower nutritional values when compared to fresh fruits. As an soluble dietary fibre (SDF). The TDF residue was filtered
example, jams normally have lower vitamin C content com- before washing with ethanol as well as acetone. The residue
pared to the fresh fruits due to exposure to the heat generated was dried before being weighed. The TDF values were calcu-
during processing (Jawaheer et al., 2003). Fruits such as lem- lated after subtraction of protein, ash and reagent blank
ons, cranberries, apples and apricots were commonly used in [TDF = weight of residue – weight (protein + ash)].
the production of jams (Burkill, 1997). Common fruit jams The ash content was estimated using dry ashing method
available in Malaysia are grape, apricot, blueberry and straw- (AOAC, 2005). Briefly, a clean silica crucible was placed into
berry. To our knowledge the nutritional contents of fruit jams a muffle furnace for 5 h. The crucible was cooled for 1 h in a
consumed in Malaysia are not yet reported. The objective of desiccator before being weighed until constant weight is
this study was to evaluate the nutritional composition of four achieved. Finely ground sample (3–5 g) was weighed into cru-
common fruit jams available in Malaysian market. The data cible. The sample was pre-ashed by heating the crucible on a
can be utilized to contribute to the enhancement of Malaysia hot plate. The crucible was then transferred to a muffle furnace
Food Composition Database. for 6–8 h. After the ashing process is completed, the crucible
was cooled before being weighed to compute the ash content.
2. Materials and methods
2.3. Mineral analysis
2.1. Sample collection and preparation
Calcium (Ca), iron (Fe), magnesium (Mg), sodium (Na), zinc
Different brands (n = 6) of grape, blueberry, strawberry and (Zn) and copper (Cu) were analyzed using Inductively
apricot jams were stratified randomly sampled from local super- Coupled Plasma Optical Emission Spectrometry (ICP-OES)
markets in Klang Valley, Kuala Lumpur, Malaysia. The sam- (Perkin Elmer, USA). Finely ground sample (3 g) was digested
ples were confirmed to represent brands consumed and using a dry ashing method (AOAC, 2005). The resulting ash
available nationwide. Each kilogram of the same brand of jams was then dissolved in concentrated hydrochloric acid (7 ml)
was mixed thoroughly and homogenized into fine form using a before being diluted to 100 ml with deionized water. The solu-
food grinder. The samples were transferred and stored into plas- tion was filtered and the mineral content was determined using
tic airtight containers at room temperature (25 ± 2 C) for up to ICP-OES.
two weeks. The replicate analysis of proximate, mineral, vita-
min, sugar and fatty acids component of lipids was carried out 2.4. Vitamin analysis
for each of the six brands of fruit jams. One pooled set of data
of mean for each reported nutrient was calculated based on Vitamin C was analyzed by using a reverse phase High
the average of the six brands tested. Performance Liquid Chromatography (HPLC) coupled with
The nutritional composition of fruit jams 91

a Diode Array detector. Based on the method described by monitor the quality of the resulting data for each sample.
Brause et al. (2003), sample (5 g) was weighed into a 100 ml Results for all nutrients analyzed were only accepted if they
flask. The sample was dissolved and acidified in 20 ml Tris were within the control limits of the quality control material.
(2-carboxyethyl)-phosphine hydrochloride acid solution. The
solution was diluted to 100 ml with 1% TCA solution and 2.8. Statistical analysis
was shaken. The resulting mixture was filtered using a
0.45 lm membrane filter into a HPLC vial for the determina- Data were analyzed using Statistical Package for Social
tion of vitamin C content. Science (SPSS) version 18.0 for Windows. All results were
Thiamine (B1), riboflavin (B2), niacin (B3), folic acid (B9) reported as mean, standard error of mean (SEM) and range.
and pyridoxine (B6) were analyzed by HPLC with Diode Significance was accepted at probability P < 0.05. The means
Array and Fluorescent detector. Sample (6 g) was weighed into and median difference between groups were compared using
a 100 ml flask. The sample was dissolved in 30 ml warm water Kruskal–Wallis t-test (normally distributed data) and Mann–
followed by the addition of 0.6 M TCA to remove any protein Whitney test (not normally distributed data).
present in the sample (Woollard and Indyk, 2002). The mix-
ture was shaken on a mechanical shaker for 15 min and the 3. Results and discussion
resulting mixture was filtered using a 0.45 lm membrane filter
into a HPLC vial. B1, B3 and B9 contents were detected using
3.1. Proximate compositions
Diode Array detector. B2 and B6 contents were detected by
using a Fluorescence detector.
Vitamin E (fat soluble vitamin) was analyzed by HPLC with The energy provided by the jams was rather high (266–
Diode Array and Fluorescent detector. Sample (5 g) was 274 kcal/100 g) (Table 1). Strawberry jams provide the highest
weighed into a 250 ml flask. Pyrogallol acid was added as an energy while the grape jams gave the lowest even though these
antioxidant followed by the addition of 40 ml of 95% ethanol. differences are not different statistically. Strawberry only pro-
The solution was then saponified with 10 ml of 50% potassium vides approximately 4.89 kcal/100 g (Giampieri et al., 2012) of
hydroxide (KOH). Glacial acetic acid (10 ml) was added in energy which is much lesser than reported in this study for
order to neutralize the KOH (DeVries and Silvera, 2002). strawberry jam. The difference in the energy content between
Vitamin E was extracted into a mixture of tetrahydrofuran both the strawberry and strawberry jam could be attributed
(THF) and ethanol (1:1) solution. The extract was filtered using to the addition of sugar during the jam making process as it
a 0.45 lm membrane filter into a HPLC vial for analysis. is a great source of energy (389 kcal/100 g) (US Department
of Agriculture (USDA, 2011).
2.5. Sugars analysis All of the jams possessed similar levels of moisture content
(31.23–33.36%). Strawberry jam had the lowest moisture con-
tent followed by apricot and blueberry jams. On the other
The total sugars, sucrose, glucose, fructose, lactose and mal-
hand, grape jams have the highest moisture content even
tose contents were analyzed using HPLC coupled with
though these differences are not statistically significant
Evaporative Light Scattering Detector (ELSD). Sample (4 g)
(p > 0.05). The moisture contents for the fruit jams were com-
was weighed into a 100 ml volumetric flask. The sample was
parable with that for roselle jam which has been reported to
dissolved into approximately 25 ml of water before further
contain between 33% and 34% (Ashaye and Adeleke, 2009).
dilution to 100 ml. Sample was filtered using a 0.45 lm mem-
Generally, the moisture content of foods can be used as an
brane filter before injected into HPLC.
indicator of its shelf life (Fellows, 2000). Low moisture content
indicates that the jams have a long shelf life.
2.6. Fatty acids analysis
For protein content, grape jams have the lowest while apri-
cot jams have the highest (Table 1) which is comparable to the
The saturated, monounsaturated, polyunsaturated and trans- protein content of jackfruit (0.19 g/100 g) and pineapple jam
fatty acids were determined using Gas Chromatography (GC) (0.46 g/100 g) (Eke-Ejiofor and Owuno, 2013). According to
coupled with Flame Ionization Detector (FID) (AOAC, the jams nutrition labelling, common ingredients are fruits,
2005). Triglycerides and phospholipids were first converted into sugar, pectin and citric acid. None of the ingredients used
Fatty Acid Methyl Esters (FAME) by saponification and ester- are an abundant source of protein; hence, the low protein con-
ification in the presence of Boron Trifluoride. The resulting sub- tent of jams is found in this study. The difference in the protein
stance was then analyzed using GC coupled with FID (Agilent content for all four types of jams analyzed was statistically sig-
Technologies, USA). The GC with FID was equipped with DB- nificant (p < 0.05).
WAX capillary column (30 m · 0.25 mm, film thickness The majority of the analyzed jams generally have very low
0.25 lm). Helium was chosen as the carrier gas. The total fat fat content with the apricot jam having no fat content what-
content was calculated as a sum of individual fatty acids. The ever while the strawberry, blueberry and grape jams have simi-
saturated, monounsaturated, polyunsaturated and trans fatty lar fat contents (0.01 g/100 g to 0.03 g/100 g). It was reported
acids were calculated as the sum of the respective fatty acids. that apricot, strawberry, blueberry and grape have very low
fat content (0.1–0.2 g/100 g) (Food Standards Australia New
2.7. Quality control Zealand (FSANZ), 2010) which explains the lack of fat
detected in jams for this study.
Internal quality control materials prepared in the laboratory All of the jams have almost similar carbohydrate content
and NIST Standard Reference Material (SRM) 1849a (65.99–67.65 g/100 g) with grape and strawberry jams contain-
Infant/Adult Nutritional Formula were used as control to ing the lowest and highest, respectively. It was reported that
92 M.N. Mohd Naeem et al.

Table 1 Proximate composition of fruit jams (g/100 g).


Parameters Type of jams p-Value
Grape Apricot Blueberry Strawberry
Mean ± SEM (range) Mean ± SEM (range) Mean ± SEM (range) Mean ± SEM (range)
Energy (kcal) 266.13 ± 32.51 272.49 ± 9.52 271.92 ± 14.21 273.89 ± 11.41 0.4
(103.92–305.56) (227.88–287.66) (225.260–306.980) (225.98–298.78)
Moisture (%) 33.36 ± 8.13 31.48 ± 2.25 31.83 ± 3.50 31.23 ± 2.83 0.43
(23.63–73.94) (27.91–42.01) (23.28–43.61) (24.79–43.22)
Protein 0.27 ± 0.08 0.43 ± 0.04 0.31 ± 0.04 0.41 ± 0.06 0.04*
(0.13–0.63) (0.27–0.53) (0.23–0.52) (0.28–0.70)
Fat 0.02 ± 0.02 N.D 0.01 ± 0.01 0.03 ± 0.02 0.55
(0.00–0.12) (N.D–0.06) (N.D–0.09)
Carbohydrate 65.99 ± 8.12 67.21 ± 2.52 67.25 ± 3.59 67.65 ± 2.91 0.36
(25.48–75.93) (55.43–71.29) (55.77–76.00) (55.56–73.75)
Total dietary fibre 0.09 ± 0.08 0.54 ± 0.26 0.40 ± 0.25 0.38 ± 0.23 0.55
(0.01–0.49) (N.D–1.69) (N.D–1.22) (N.D–1.29)
Ash 0.18 ± 0.04 0.25 ± 0.03 0.12 ± 0.02 0.23 ± 0.03 0.04*
(0.10–0.30) (0.10–0.30) (0.10–0.20) (0.20–0.40)
SEM – Standard Error Mean.
N.D – Not detected.
*
p < 0.05 are significant differences for Kruskal–Wallis test between various types of jams.

carbohydrate content ranged from 14 g/100 g to 48 g/100 g for that variations in geographical area and soil from which the
pineapple and jackfruit jams (Eke-Ejiofor and Owuno, 2013). strawberry was collected contribute to the differences seen.
Overall, carbohydrate consists of 66–68% of the nutrients in The differences could also be linked to the addition of calcium
all of the jams analyzed. High carbohydrate content in jams chloride as a firming agent to solidify the jams (Luna-Guzmán
can be associated with the large presence of sugar (>50 g/ and Barrett, 2000).
100 g) as observed from the nutrition labelling on its In terms of Fe content, grape and apricot jams tend to have
packaging. similar levels. However, blueberry and strawberry jams tend to
In terms of TDF, all of the jams generally have low TDF contain higher Fe levels. National Institute for Health and
content with grape jam having the lowest TDF content. On Welfare Finland (THL), (2013) also reported the Fe content
the other hand, strawberry and blueberry jams have nearly of strawberry jam to be similar as reported in this study. The
identical TDF content while the apricot jams contained the level of Fe in the analyzed jams was comparable to that
highest TDF content. Department of Health UK (DH) reported in the fruits itself (Panceri et al., 2013; FSANZ,
(2013) reported that grape, strawberry and blueberry have 2010; DH, 2013). Grape, apricot, blueberry and strawberry
low TDF content (1.3–3.8 g/100 g). This is consistent with are not abundant with Fe which explains the low Fe content
TDF content of other fruits such as orange, plum and guava for its jams in this study.
(0.3–5.2 g/100 g) as reported by Ramulu and Rao (2003). Strawberry jam also tends to have the highest Mg content
Fruits contained low TDF content which explains the scarcity even though blueberry jam had the lowest. The level of Mg
of TDF in all of the jams. in strawberry jam was significantly different (p < 0.05) from
The ash content of the jams ranged from 0.12 g/100 g to other analyzed jams. Giampieri et al. (2012) reported the Mg
0.25 g/100 g which is comparable to that of pineapple, jack- content of strawberry (13 mg/100 g) to be approximately twice
fruit and mango jams (0.15–0.49 g/100 g) (Eke-Ejiofor and that was reported for strawberry jam in this study. The lower
Owuno, 2013; Kansci et al., 2003). Apricot jam had the highest Mg content for strawberry jam found in the present study
ash content while the blueberry jam had the lowest and the dif- could be attributed to the dilution of fruit pulp with sugar dur-
ferences were found to be statistically significant. The findings ing jam making process (Plessi et al., 2007). THL (2013)
were comparable to the ash content of apricot jam (0.2 g/ reported that strawberry contained higher Mg content
100 g) (FSANZ, 2010; Sartaj et al., 2011). Generally, low ash (15 mg/100 g) compared with apricot and grape (8.8–9 mg/
content indicates that all of the jams analyzed are not a rich 100 g). This explains the higher Mg content for strawberry
source of minerals. jam compared with other jams in this study.
Strawberry jam had the lowest Na content followed by
grape jam (4.07 mg/100 g). On the other hand, apricot and
3.2. Mineral compositions blueberry jams have significantly higher but almost similar
Na contents. In comparison, Panceri et al. (2013) reported
Apricot and blueberry jams (Table 2) tend to have lower Ca lower Na content of grape berries (0.13–3.41 mg/100 g). It
levels when compared to grape and strawberry jams. Ca con- was also reported that apricot, strawberry and blueberry have
tent of the jams was comparable to the one reported for straw- low Na content (1–2 mg/100 g) (USDA, 2011; DH, 2013). The
berry, blueberry and grape (6–16 mg/100 g) (Giampieri et al., differences in Na content between the fruits and jams can be
2012; USDA, 2011; FSANZ, 2010). In comparison, associated with the presence of sodium citrate during jam-
Gakowska et al. (2010) reported higher Ca content (27.26– making. Sodium citrate is the sodium salt for citric acid that
55.44 mg/100 g) in the analyzed strawberry jams. It is possible functions as an acidity regulator in jams.
The nutritional composition of fruit jams 93

Table 2 Mineral composition of fruit jams (mg/100 g).


Minerals Type of jams p-Value
Grape Apricot Blueberry Strawberry
Mean ± SEM (range) Mean ± SEM (range) Mean ± SEM (range) Mean ± SEM (range)
Calcium (Ca) 10.283 ± 2.987 7.633 ± 1.207 6.517 ± 1.409 18.100 ± 7.934 0.299
(3.200–20.000) (3.000–10.800) (1.400–10.900) (5.400–57.300)
Iron (Fe) 0.200 ± 0.045 0.200 ± 0.063 0.250 ± 0.067 0.267 ± 0.062 0.728
(0.100–0.400) (0.100–0.500) (0.100–0.500) (0.100–0.500)
Magnesium (Mg) 3.633 ± 1.130 4.250 ± 0.316 2.717 ± 0.251 6.783 ± 0.633 0.010*
(1.300–7.600) (2.900–5.300) (2.000–3.400) (4.600–9.400)
Sodium (Na) 4.066 ± 0.398 8.917 ± 3.404 9.233 ± 1.596 1.367 ± 1.367 0.006*
(3.200–5.700) (3.600–25.500) (5.000–16.000) (N.D–8.200)
Zinc (Zn) 0.070 ± 0.054 0.010 ± 0.010 N.D 0.048 ± 0.034 0.395
(N.D–0.330) (N.D–0.060) (N.D–0.200)
Copper (Cu) 0.003 ± 0.002 0.023 ± 0.002 0.017 ± 0.007 0.013 ± 0.003 0.005*
(N.D–0.010) (0.020–0.030) (N.D–0.050) (0.010–0.030)
SEM – Standard Error Mean.
N.D – Not detected.
*
p < 0.05 are significant differences for Kruskal–Wallis test between various types of jams.

All of the fruit jams analyzed have very low content of Zn B1 content of guava to be of similar range (0.06 mg/100 g).
detected with the blueberry jam which have no Zn content at Low content of vitamin B1 in fruits could explain the absence
all. Muchuweti et al. (2011) reported that the Zn content of and very low level of vitamin B1 in jams.
Parinari curatellifolia jams is also very low (0.02 mg/100 g). In terms of vitamin B2, none was detected for all of the fruit
Strawberry and grape jams have higher but similar Zn content. jams analyzed. THL (2013) reported that strawberry jam had
Grape, apricot and strawberry have low Zn content as very low vitamin B2 content (0.02 mg/100 g). In addition,
reported by THL (2013) (0.1–0.3 mg/100 g). This explains the grape, apricot, strawberry and blueberry had low vitamin B2
very low Zn content of fruit jams reported in this study. The content (0.02–0.07 mg/100 g) (USDA, 2011). Vitamin B2 could
differences in the Zn content of fruits and jams could be traced have been damaged during jam-making process when heat
to the possible destruction of Zn caused by heat treatment dur- treatment involved. This explains the non-detection of vitamin
ing jam-making process. B2 for jams in this study.
Cu was not detected in grape jam but was present in very Vitamin B3 was not detected in blueberry and strawberry
low amounts (0.01–0.02 mg/100 g) in other fruit jams ana- jams. On the contrary grape and apricot jams contained sig-
lyzed. In comparison, Acar (2011) reported higher Cu content nificantly higher (0.16 mg/100 g and 22.03 mg/100 g) of vita-
in apricot jam (0.08 mg/100 g). It was also reported by FSANZ min B3 respectively. The result is consistent with the findings
(2010) that grape, strawberry and blueberry have low Cu con- of Leccese et al. (2011) who reported that apricot is a rich
tent (0.065–0.24 mg/100 g). Grape, apricot, strawberry and source of vitamin B3. Vitamin B3 is essential in the treatment
blueberry are not a rich source of Cu and it is reflected in of cardiovascular disease and preventing progression of ather-
the Cu content of jams analyzed in this study. osclerosis (Majewski and Lebiedzińska, 2014).
Vitamin B6 was not detected in any of all the jams. DH
3.3. Vitamin compositions (2013) reported that grape, strawberry and blueberry had very
low vitamin B6 content (0.01–0.04 mg/100 g). Jam-making
Grape and blueberry jams had similar vitamin C contents process caused losses of bioactive compounds and sensory
while apricot jam had the highest vitamin C content properties (Vibhakara and Bawa, 2006). This was corrobo-
(Table 3). On the other hand, strawberry had the highest vita- rated by the non-detection of vitamin B6 in all of the jams.
min C content while grape had the lowest (9.7–58.8 mg/100 g) Vitamin B9 was not detected in grape and strawberry jams.
(USDA, 2011). The differences in vitamin C content between However apricot and blueberry jams had significantly higher
fruits and jams could be linked to the destructive nature of vitamin B9 contents (14.02 and 14.63 lg/100 g) respectively.
jam-making process. Processing of fruits into jams was In addition, vitamin B9 content of apricot jam is similar to
revealed to be most damaging towards vitamin C (Uckiah apricot (14 lg/100 g) (THL, 2013). The absence of vitamin
et al., 2009) which explains low vitamin C content in jams in B9 in grape and strawberry jams could be caused by the
this study. Valente et al. (2014) reported that vitamin C is inte- destruction of nutrients during jam-making process. Vitamin
gral in biochemical process in human body. In addition, it is B9 is important for neurological function and its deficiency is
also extensively used as an antioxidant and food additive. linked with higher occurrence of mental symptoms and cogni-
Vitamin B1 was not detected in grape and apricot jams. On tive drop in epileptic, neurological, psychiatric, and elderly
the other hand blueberry and strawberry jams had high vita- populations (Kronenberg et al., 2009; Rosa et al., 2014).
min B1 content with the latter having the highest level. The Strawberry jam had the lowest vitamin E content (0.24 mg/
findings were comparable to vitamin B1 content of grape, apri- 100 g). Similarly, Giampieri et al. (2012) reported that the vita-
cot, strawberry and blueberry (0.02–0.03 mg/100 g) (FSANZ, min E content of strawberry jams is 0.29 mg/100 g. Blueberry
2010). Likewise Ismail et al. (2013) reported that the vitamin jam had the highest vitamin E content even though there were
94 M.N. Mohd Naeem et al.

Table 3 Vitamin composition of fruit jams (mg/100 g).


Vitamins Type of jams p-Value
Grape Apricot Blueberry Strawberry
Mean ± SEM (range) Mean ± SEM (range) Mean ± SEM (range) Mean ± SEM (range)
C 2.65 ± 0.07 5.67 ± 2.78 2.52 ± 0.10 3.28 ± 0.69 0.401
(2.50–2.90) (N.D–18.4) (2.40–3.00) (2.30–6.70)
B1 N.D N.D 0.06 ± 0.06 0.09 ± 0.09 0.553
(N.D–0.38) (N.D–0.52)
B2 N.D N.D N.D N.D 1.000
B3 0.16 ± 0.08 22.03 ± 8.69 N.D N.D 0.000*
(N.D–0.52) (3.40–61.60)
B6 N.D N.D N.D N.D 1.000
B9 (lg/100 g) N.D 14.02 ± 6.61 14.63 ± 4.10 N.D 0.001*
(N.D–43.90) (2.02–30.66)
E 0.33 ± 0.19 0.44 ± 0.17 0.62 ± 0.20 0.24 ± 0.08 0.386
(N.D–1.01) (0.10–1.28) (N.D–1.44) (0.10–0.63)
SEM –Standard Error Mean.
N.D – Not detected.
*
p < 0.05 are significant differences for Kruskal–Wallis test between various types of jams.

no significant differences between vitamin E content in all of environmental variations, harvest of apricot and ripening
the fruit jams analyzed. DH (2013) reported that blueberry levels (Akin et al., 2008).
had higher vitamin E content than its jam in this study Strawberry jams have the lowest fructose content (15.77 g/
(1 mg/100 g). Vitamin E is essential to human body as an 100 g) but grape jams have the highest. In comparison, the
antioxidant and for guarding cell membranes against peroxi- fructose content in strawberry fruits was reported to be much
dation (Lima et al., 2014). lower (2.44 g/100 g) (Giampieri et al., 2012). This difference
may be attributed to the addition of high fructose corn syrup
3.4. Sugar compositions as listed on the packaging of the jams analyzed. The fructose
content obtained for apricot jam was also similar to that pre-
All the fruit jams have similar total sugar contents ranging viously reported (21.34 g/100 g) (Touati et al., 2014). No lac-
from 52.43 g/100 g to 54.78 g/100 g (Table 4). Grape jam had tose was detected in all the jams. This is consistent with the
the lowest total sugar contents while blueberry jam had the absence of lactose for grape, apricot and strawberry
highest. Grape, apricot, strawberry and blueberry had con- (FSANZ, 2010). Lactose usage in food product is limited
siderably lower total sugar contents than jams (4.89–17 g/ because of its low solubility, minimal sweetness and risk of lac-
100 g) (Giampieri et al., 2012; DH, 2013). The noticeable dif- tose intolerance (Pessela et al., 2003).
ferences could be caused by the addition of sugar during Grape jam did not contain any maltose even though straw-
jam-making process (Igual et al., 2013). The addition of sugar berry jams had the highest level (9.68 g/100 g). It was reported
is essential in order to preserve the jams satisfactorily. In com- by DH (2013) that grape, strawberry and blueberry showed no
parison, Touati et al. (2014) reported that total sugars for apri- presence of maltose. It is possible that maltose was detected in
cot jam to be higher (64.88 g/100 g) than reported in this study. some of the fruit jams due to the addition of maltitol during
The variation may be caused by different varieties of apricot jam-making process as observed from the packaging.
used for the jams which was not investigated in this study. Maltitol is made by hydrogenation of maltose and mainly
Grape jams have the lowest sucrose content at 1.02 g/100 g. functions as a sweetener even though it is less sweet than glu-
This was followed by blueberry and apricot jams at 5.08 g/ cose or fructose (Jia et al., 2008).
100 g and 8.47 g/100 g respectively while strawberry jams have
the highest sucrose content (9.78 g/100 g). However, FSANZ 3.5. Fatty acids
(2010) stated that there was an absence of sucrose in straw-
berry. The presence of sucrose may be attributed to its addi- The fat content in the apricot jam was less than 0.02 g/100 g.
tion during jam-making as listed on the packaging of jams Therefore, no fatty acid analysis was conducted for apricot
analyzed. Sucrose helps in promoting pectin gelation during jam. The total saturated fatty acids for grape, blueberry and
jam-making process (Bulone et al., 2010). strawberry jams were similar (0.01–0.02 g/100 g). Total satu-
Grape jams had 26.2 g/100 g of glucose detected. On the rated fatty acids were comparable with the one reported for
other hand, strawberry jam had 17.42 g/100 g of glucose con- strawberry jam (<0.1 mg/100 g) (THL, 2013). FSANZ
tent while apricot and blueberry jams had almost similar glu- (2010) reported that there was no presence of total fatty acids
cose contents. However, glucose content for all fruit jams for grape, apricot and strawberry. However, USDA (2011)
analyzed was not significantly different. Again, Touati et al. reported low total fatty acids for grape, apricot and strawberry
(2014) reported higher glucose content in apricot jams (0.1–0.2 g/100 g). The differences may be attributed to the dif-
(21.04 g/100 g) which may be contributed by genetic, ferent varieties of fruits used for jams. In addition, climate and
The nutritional composition of fruit jams 95

Table 4 Sugar composition of fruit jams (g/100 g).


Vitamins Type of jams p-Value
Grape Apricot Blueberry Strawberry
Mean ± SEM (range) Mean ± SEM (range) Mean ± SEM (range) Mean ± SEM (range)
Total sugar 52.43 ± 9.48 53.65 ± 1.96 54.78 ± 2.83 52.67 ± 1.94 0.806
(7.80–70.50) (48.00–59.60) (48.60–66.10) (44.70–58.10)
Sucrose 1.02 ± 1.02 8.47 ± 4.60 5.08 ± 4.32 9.78 ± 4.72 0.237
(N.D–6.10) (N.D–30.10) (N.D–26.40) (N.D–26.40)
Glucose 26.20 ± 4.49 23.33 ± 1.78 23.73 ± 1.70 17.42 ± 3.11 0.085
(4.50–32.80) (15.70–28.40) (17.00–29.50) (4.80–25.80)
Fructose 26.00 ± 4.69 19.85 ± 1.94 21.72 ± 1.650 15.77 ± 2.80 0.058
(3.30–32.80) (13.80–25.10) (16.60–26.10) (4.40–24.40)
Lactose N.D N.D N.D N.D 1
Maltose N.D 2.00 ± 1.34 4.27 ± 2.34 9.68–5.53 0.105
(N.D–7.70) (N.D–14.50) (N.D–35.50)
SEM – Standard Error Mean.
N.D – Not detected.
p < 0.05 are significant differences for Kruskal–Wallis test between various types of jams.

soil conditions where the fruits were obtained could contribute flame atomic absorption spectrometry. Eurasian J. Anal. Chem. 6,
to the differences as well. Palmitic acid (C16:0) was detected 114–128.
for grape, blueberry and strawberry jams. On the other hand, Akin, E.B., Karabulut, I., Topcu, A., 2008. Some compositional
stearic acid (C18:0) was detected for grape jams. properties of main Malatya apricot (Prunus armeniaca L.)
varieties. Food Chem. 107, 939–948.
AOAC, 2005. Official Methods of Analysis of AOAC International,
4. Conclusion 18th ed. AOAC International, Maryland, USA.
AOAC, 2006. Official Methods of Analysis of AOAC International,
In conclusion, this study has shown that fruit jams (grape, 18th ed. 1st Revision. AOAC International, Maryland, USA.
apricot, blueberry and strawberry) provide a good source of AOAC, 2008. Official Methods of Analysis of AOAC International,
18th ed. 2nd Revision. AOAC International, Maryland, USA.
energy and carbohydrate. The fruit jams have very low levels
Ashaye, O.A., Adeleke, T.O., 2009. Quality attributes of stored Roselle
of fatty acids. These findings will provide valuable nutritional
jam. Int. Food Res. J. 16, 363–371.
composition data of fruit jams that are available in Malaysia Brause, A.R., Woollard, D.C., Indyk, H.E., 2003. Determination of
which could help healthcare professionals in assisting the pub- total vitamin C in fruit juices and related products by liquid
lic to make healthier food choices. Furthermore, it could also chromatography: interlaboratory Study. J. AOAC Int. 86,
be utilized to improve the Malaysian Food Composition 367–374.
Database. Bulone, D., Giacomazza, D., Manno, M., Martorana, V., Biagio,
P.L.S., 2010. Sucrose pectin interaction from solution to gel. In:
Conflict on interest Hollngworth, C.S. (Ed.), Food Hydrocolloids: Characteristics,
Properties and Function. Nova Science Publishers Inc., New York,
pp. 225–241.
The authors have declared no conflict of interest. Burkill, H.M., 1997. The Useful Plants of West Tropical Africa. Royal
Botanic Gardens, Kew, UK.
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Department of Chemistry, Staff of the Food properties of jackfruit (Artocarpus heterophilus) jam. Int. J. Nutr.
Section Department of Chemistry Malaysia and staff of the Food Sci. 2, 149–152.
Nutrition Unit, Institute for Medical Research for their Fellows, P.J., 2000. Food Processing Technology, Principles and
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the National Institute of Health and the Institute for FSANZ, 2010. FSANZ Nutrient Table for Use in Australia
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