Department of Zoology

Alipurduar College
Alipurduar
Paper IV: Cell Biology; Molecular Biology; Laboratory and Analytical Techniques and
Biochemistry
Time: 4hours Full Marks: 90

Group A: Cell Biology

1. Answer the following questions: 1X6=6
a. What is integral protein?
b. What is F1 particle?
c. What is the function of vacuoles’?
d. What are microfilaments?
e. What is nuclear lamina?
f. What are the phases of cell cycle?
g. What is the function of nucleolus?
h. What are the major activities of peroxisomes?
2. Answer the following questions: 3X3=9
a. Write a short note on peripheral membrane protein.
b. Write a note on COPII-Coated Vesicles.
c. Describe three distinct roles of lysosomes.
d. Describe three functions of actin filaments.
e. Describe the check points of cell cycle.
3. Answer the following questions: 10X1=10
a. What is sarcomere? Describe the structure of sacromere. Write a short note on
mechanism of muscle contraction. 2+4+4=10
b. Describe the fluid-mosaic model for the structure of plasma membrane. What is
the function of plasma membrane? 7+3=10
Group B: Molecular Biology
4. Answer the following questions: 1X4=4
a. What are modified bases? Give example.
b. Why is RNA molecules more susceptible to hydrolysis than DNA?
c. Differentiate between synthase and synthetase.
d. What is P-site and A-site?
e. Distinguish between DNA polymerase and RNA polymerase.
f. What is linker DNA?
5. Answer the following questions: 3X2=6
a. Write a note on inhibitors of DNA replication.
b. Describe the cloverleaf model of tRNA.
c. State the role sigma factor in transcription.
d. Describe the process of splicing of RNA.
6. Answer the following questions: 10X1=10
a. Describe DNA excision repair mechanism.
b. Describe the activation of tRNA in protein synthesis.
 Group C: Laboratory and Analytical Techniques
7. Answer the following questions: 1X4=4
a. What is genetic engineering?
b. What is homogenization?
c. What is chromatography?
d. Migration of ions in an electric field at a definite pH is called _____________.
e. What is Svedberg unit?
f. What is restriction enzyme?
g. What is recombinant DNA?
8. Answer the following questions: 3X2=6
a. Explain the principle of spectrophotometry.
b. Write a short note on biosensors and its application.
c. Write a short note on tools of genetic engineering.
d. Write a short note on thin layer chromatography.
9. Answer the following questions: 10X1=10
a. What is DNA fingerprinting? Describe the process of DNA fingerprinting.
b. What is centrifugation? Write down the principle of centrifugation. Write a short
note on types of centrifugation.

Group D: Biochemistry
10. Answer the following questions: 1X6=6
a. What is disulfide bridge?
b. Give two examples of aromatic amino acids.
c. What is holoenzyme?
d. Which order of reaction is Michaelis-Menten enzyme kinetics?
e. What is transamination reaction?
f. What is the rate limiting steps of glycolysis?
g. What is palindromic sequence?
h. What is immobilization of enzyme?
11. Answer the following questions: 3X3=9
a. Substitution of a single amino acid may alter biological property of protein-
Comment.
b. Why fats are solid while oils are liquid at room temperature?
c. Outline the pentose sugar pathway.
d. Write a note on NADH dehydrogenase.
e. Explain the overall balance sheet of ATP formation.
12. Answer the following questions: 10X1=10
a. Describe the steps involved in β-oxidation.
b. What is enzyme kinetics? Explain Michaelis-Menten enzyme kinetics.