ASSAY OF ACETAMINOPHEN TABLETS USING HPLC QC2 LABORATORY HPLC • High Performance Liquid Chromatography • Liquid Chromatography (LC)
• A separation technique based on a solid stationary phase and a liquid
mobile phase (USP 36). HPLC • STATIONARY PHASE
Separations are achieved by partition, adsorption, or ion-exchange
processes depending on the type of stationary phase used.
Most commonly used: silica, polymeric beads
HPLC • CHROMATOGRAPHIC COLUMN
Includes stainless steel, lines stainless steel, and polymeric column,
packed with a stationary phase. The length and inner diameter of the column affects the separation, and therefore typical column dimensions are included in the individual monographs. HPLC • MOBILE PHASE
A solvent or a mixture of solvents, as defined in the individual
monograph (USP 36). HPLC • APPARATUS
A liquid chromatogram consists of (USP 36):
• Reservoir containing the mobile phase • Pump to force the mobile phase through the system at high pressure • Injector to introduce the sample into the mobile phase • Chromatographic column • Detector • Data collection device HPLC • GRADIENT ELUTION
The technique of continuously changing the solvent composition during
the chromatographic run. Experiment 3 MOBILE PHASE
To prepare, measure 250 mL HPLC-grade methanol using a volumetric
flask. Transfer to 1000 mL volumetric flask and fill to volume. Experiment 3 STANDARD SOLUTION
0.01 mg/mL of USP Acetaminophen RS in mobile phase
Experiment 3 SAMPLE SOLUTION
Weigh, and finely powder NLT 20 Tablets. Dissolve a portion of the
powder in Mobile phase to prepare a solution containing 0.01 mg/mL of acetaminophen. To facilitate dissolution, shake the powder in Mobile phase by mechanical means for 10 min, sonicate for 5 min, and dilute with Mobile phase to volume. Pass a portion of this solution through a filter of 0.5- μm or finer pore size, discarding the first 10 mL of the filtrate. Use the clear filtrate. Experiment 3 SAMPLE SOLUTION
Compute for the weight of powder containing ~100 mg of