VISAYAS STATE UNIVERSITY

BAYBAY CITY

College of Arts and Sciences

Department of Pure and Applied Chemistry

Chemistry 146
Analytical Chemistry II

2nd Semester
SY: 2016-2017
 VISAYAS SATE UNIVERSITY
BAYBAY CITY

Department of Pure and Applied Chemistry

Instructor: Mr. Genesis Albarico

Chemistry 146
Analytical Chemistry II

Experiment 5

Ultraviolet-Visible Spectroscopy
Adsorption Spectrum of Caffeine by UV Spectrophotometry

submitted by

Jerald T. Villarmino

Baybay City, Leyte, 2017

CONTENT

LIST OF FIGURES, TABLES, AND EQUATIONS…………………………………………….. v

THEORETICAL FRAMDEWORK……………………………………………………………….

1 INTRODUCTION…………………………………………………………………………………
1.1 Objectives………………………………………………………………………………………...
1.2 Scope and Limitations……………………………………………………………………………
1.3 Time and Place of the Study……………………………………………………………………..

2 REVIEW OF LITERATURE.........................................................................................................
2.1 Coffee Bean………………………………………………………………………………………
2.2 Caffeine……………………………………………………………………………………………
2.3 Health effects of caffeine…………………………………………………………………………
2.4 Methylene Chloride………………………………………………………………………………
2.5 Liquid-Liquid Extraction…………………………………………………………………………
2.6 Ultraviolet-visible Spectrophotometry…………………………………………………………..
2.6.1 UV-1800………………………………………………………………………………………..
2.7 External Calibration Curve………………………………………………………………………
2.8 Instrument Detection Limit (IDL)……………………………………………………………….
2.9 Limit of Detection (LOD)……………………………………………………………………….
2.10 Limit of Quantification (LOQ)………………………………………………………………...

3 METHODOLOGY…………………………………………………………………………………
3.1 Sample preparation………………………………………………………………………………
3.2 Preparation of calibration curve………………………………………………………………...
3.3 Analysis of caffeine using UV-Vis Spectrophotometer………………………………………..
3.4 Computation for IDL, LOD and LOQ………………………………………………………….

4 RESULTS AND DISCUSSION

4.1 Standard Solution and Calibration Curves……………………………………………………..
4.2 Caffeine Content……………………………………………………………………………….
4.3 Caffeine content of different beverages………………………………………………………..

5 SUMMARY, CONCLUSION AND IMPLICATIONS……………………………………………

LIST OF REFERENCES…………………………………………………………………………….

APPENDICES…………………….…………………………………………………………………...
Appendix A. 1 Caffeine content of coffee beans……………………………………………………….
Appendix A. 2 Blank Absorbance using UV-1800....…………………………………………………..
Appendix A. 3 Sample Absorbance using UV-1800…………………………………………………..
Appendix A. 4 LOD and LOQ of UV-1800……………………………………………………

Figures

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 Tables

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