200 µl solution C

aspirate solution C
200 µl washing solution
100 µl Solution B check check efficiency of aspiration
precision of Dispensing

25 µl solution B
25 µl solution A
low volume dispensing
1 tip per reagent Wells must be filled equally

90 µl solution A
10 µl solution C
pipetting is similar to Wells must be empty
pre-dilution - 1 tip per well
 The following criteria have to be met:
•· QC1 (dispense accuracy 100 µl, strip 1+2) CV< 2.5%

•· QC2 (dispense accuracy 25 µl, strip 3+4) CV< 5.0%

•· QC3 (pipetting accuracy 10 µl + 90µl, strip 5+6) CV< 5.0%

•· QC4 (pipetting accuracy, strip 7+8) max. < 1,2 O.D.

•· QC4 (aspiration effectiveness, strip 7+8) mean < 0,5 O.D.

•· QC5 (washer test, strip 9+10) all wells must be equally filled.

•· QC6 (washer test, strip 11+12) all wells must be completely empty.