Into an Erlenmeyer flask pipette Titrate with ascorbic acid until
Add the standard ascorbic acid there is a disapearance of pink into a burette 20 mL of dichlorophenol- indophenol sol'n colour.
Repeat the titration for atleast 3
Calculate mean and standard times,recording observation. deviation (ascorbic acid calculate mean and standard equivalence per 1 mL dye) deviation (ascorbic acid equivalence per 1 mL dye) B) ESTIMATION OF ASCORBIC ACID IN CANNED JUICE
Measure 25mL of the preparation
Into a 500mL beaker pour 2 mL using measuring cyclinder Make up volume to 50 mL by the of canned juice and acidify with tranferring to a 50 mLconical addition of distilled water and 0.2 mL glacial acetic acid,make flask,followed by addition of 5 % transfer to burette up volume with distilled water. meta phosphoric acid.
In mg per mL of canned orange For the dichlorophenol
juice calculate ascorbic acid -indophenol solution titrate content. atleast three (3) ,5 mL volumes. C) EXTRACTING DNA FROM BANANAS
Mix 2mL od soap with 0.5g
salt in small ziplock bag + Put 100mL of distilled water 4mL of distilled water .Mix Add 10 mL of banana mix for an approx. 50g banana in gently to avoid creating atleast 5 mins. ziplock bag and crush. foam ; continue until soap and salt dissolve.
Insert a filter in a clean plastic
Place test tube with alcohol Remove the filter and set aside cup so it doesn't touch the and filtrate it in a test tube then get a test tube of cold bottom of cup + pour mixture holder,let it sit undisturbed for alcohol ,use a pipette or from step 3 into filter,after 5 4 mins.Dip glass rod to spool eyedropper to collect fiiltrate mins,some liquid called filtrate out banana's DNA. and add it to alcohol. should have collectted at the bottom.