Beruflich Dokumente
Kultur Dokumente
NORMALIZED DATA
Wavelength buffer 1 buffer 2 buffer 3 buffer 4
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
Absorbance difference
wavelengths)
Reference:
"Rapid Determination of Ionization Constants (pKa) by
C.H. Ríos Martínez, C. Dardonville
ACS Med. Chem. Lett.. 2013, 4, 142-145.
http://pubs.acs.org/articlesonrequest/AOR-TV3vU6DJ3
http://hdl.handle.net/10261/82654
Raw dat
12
10
Absorbance
2
4
0
250 300 350
Waveleng
buffer 11 buffer 12
0 0
0 0
0 0
0 0
0 0
0 0
0 0
Norm
0 0
0 0
12
0 0
0 0
0 0
0 0
10
0 0
Absorbance
0 0
0 0
0 0 8
0 0
0 0
0 0
0 0 6
0 0
0 0
0 0
0 0 4
0 0
0 0
0 0
0 0 2
0 0
0 0
0 0
0 0 0
0 0 250 300 350
0
250 300 350
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
buffer 11 buffer 12
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0 Spectral dif
0 0
0 0
0 0 3.5
0 0
0 0 3
0 0
0 0 2.5
0 0
0 0 2
Absorbance
0 0
0 0 1.5
0 0
0 0 1
0 0
0 0 0.5
0 0
0 0 0
0 0
0 0 -0.5
0 0
-1
-1.5
0
-0.5
0 0 -1
0 0
0 0 -1.5
0 0
0 0 -2
0 0
0 0 -2.5
0 0
0 0 -3
0 0
0 0 -3.5
250 270 290 310 330 350 370
0 0
0 0 Waveleng
318
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
bsorbance difference vs pH
pKa Determination
5 8 11 14
pH
y (pKa) of aryl guanidines and 2-(arylimino)imidazolidines: correlations of pH-metric and UV
arro de la Fuente, G. Martín Herranz, B. Corrales Mariblanca, P. L. A. Popelier
/C7NJ02497E
uest/AOR-TV3vU6DJ386KArNmw9jF
Raw data
350 400 450 500
Wavelength (nm)
Normalized data
Column
B
Column
C
Column
D
Column
E
Column
F
Column
G
Column
H
Column
I
Column
J
Column
K
Column
Column
H
Column
I
Column
J
Column
K
Column
L
Column
M
Column
C
Column
D
Column
E
Column
F
Column
G
Column
H
Column
I
Column
J
Column
K
Column
Column
H
Column
I
Column
J
Column
K
Column
L
Column
M
490
initio bond lengths
RAW DATA (pH of buffer solutions used in the experiment)
Wavelength 2.68 5.09 6.34 6.8
230 0.82695 0.80767 0.82671 0.90198
232 1.12480 1.14879 1.03912 1.05687
234 1.16201 1.15358 1.10788 0.99585
236 1.15271 1.10659 1.11211 0.98860
238 1.13032 1.02602 1.07619 0.96851
240 1.07511 0.95419 1.02150 0.96331
242 1.01048 0.91548 0.95313 0.92803
244 0.95152 0.88226 0.90023 0.88983
246 0.85931 0.79934 0.81676 0.82251
248 0.80954 0.76093 0.77135 0.78414
250 0.75468 0.72052 0.71405 0.74027
252 0.67688 0.64988 0.64401 0.67513
254 0.64472 0.62793 0.61167 0.65108
256 0.60512 0.59010 0.57197 0.60954
258 0.57903 0.56956 0.54652 0.58300
260 0.57415 0.56885 0.54160 0.57465
262 0.57715 0.57474 0.54124 0.57350
264 0.59316 0.59424 0.55632 0.58750
266 0.62044 0.61828 0.57831 0.60745
268 0.64492 0.64107 0.59974 0.62879
270 0.67525 0.67189 0.62573 0.65287
272 0.70231 0.69671 0.64886 0.67617
274 0.72416 0.71658 0.66767 0.69599
276 0.74843 0.73670 0.68534 0.71571
278 0.76875 0.76062 0.70612 0.73480
280 0.79163 0.77846 0.72397 0.75311
282 0.80491 0.79102 0.73642 0.76653
284 0.81677 0.80342 0.74647 0.77819
286 0.82649 0.81176 0.75340 0.78479
288 0.82964 0.81613 0.75730 0.78956
290 0.83268 0.82056 0.76088 0.79321
292 0.84456 0.81912 0.76002 0.79476
294 0.83120 0.81595 0.75826 0.79417
296 0.82782 0.81452 0.75530 0.79265
298 0.82343 0.81081 0.75158 0.78984
300 0.81653 0.80379 0.74608 0.78468
302 0.80709 0.79609 0.73868 0.77739
304 0.79488 0.78525 0.72830 0.76891
306 0.78311 0.77498 0.71838 0.75877
308 0.76794 0.76030 0.71024 0.74672
310 0.75049 0.74457 0.69230 0.73254
312 0.73310 0.72663 0.67655 0.71882
314 0.71214 0.70976 0.65869 0.70205
316 0.68849 0.68503 0.63766 0.68129
318 0.66615 0.66510 0.61990 0.66374
320 0.64328 0.64279 0.60051 0.64340
322 0.61621 0.61658 0.57498 0.61937
324 0.59234 0.59465 0.55533 0.59866
326 0.56708 0.56703 0.53278 0.57780
328 0.54460 0.54400 0.51254 0.55585
330 0.52348 0.52243 0.49243 0.53754
332 0.49729 0.49738 0.46932 0.51438
334 0.47547 0.47517 0.44879 0.49458
336 0.45346 0.45104 0.42871 0.47501
338 0.43038 0.42906 0.40609 0.45299
340 0.40942 0.40740 0.38828 0.43607
342 0.38903 0.38660 0.37067 0.41762
344 0.36706 0.36575 0.35100 0.39751
346 0.33914 0.34046 0.32742 0.37433
348 0.32135 0.32191 0.31189 0.35926
350 0.30397 0.30363 0.29371 0.34277
352 0.28741 0.28779 0.28106 0.33075
354 0.26742 0.26908 0.26498 0.31412
356 0.25276 0.25501 0.25076 0.30102
358 0.23788 0.24024 0.23721 0.28941
360 0.22036 0.22197 0.22327 0.27399
362 0.20668 0.20951 0.21190 0.26347
364 0.19236 0.19644 0.19842 0.25223
366 0.17642 0.18066 0.18423 0.23702
368 0.16425 0.16914 0.17385 0.22605
370 0.15010 0.15336 0.16165 0.21373
372 0.14074 0.14347 0.15192 0.20394
374 0.12716 0.13438 0.14057 0.19428
376 0.11720 0.12280 0.13258 0.18531
378 0.10792 0.11336 0.12390 0.17618
380 0.09847 0.10280 0.11402 0.16523
382 0.08814 0.09517 0.10529 0.15655
384 0.08115 0.08561 0.09751 0.14893
386 0.08135 0.07756 0.08988 0.13980
388 0.06569 0.07220 0.08366 0.13114
390 0.05711 0.06414 0.07652 0.12313
392 0.05220 0.05819 0.07019 0.11685
394 0.04495 0.05134 0.06395 0.10920
396 0.04420 0.04560 0.05837 0.10329
398 0.03561 0.04058 0.05383 0.09601
400 0.03035 0.03439 0.04840 0.08868
402 0.02631 0.03131 0.04493 0.08266
404 0.02248 0.02719 0.03928 0.07697
406 0.01946 0.02483 0.03580 0.07275
408 0.01689 0.02071 0.03204 0.06664
410 0.02991 0.01864 0.02832 0.06235
412 0.01314 0.01666 0.02584 0.05845
414 0.01232 0.01490 0.02311 0.05391
416 0.00969 0.01396 0.02085 0.05061
418 0.00829 0.01244 0.01827 0.04592
420 0.00787 0.01107 0.01741 0.04365
422 0.00711 0.00986 0.01473 0.03925
424 0.00639 0.00972 0.01304 0.03737
426 0.00750 0.00926 0.01230 0.03396
428 0.00692 0.00892 0.01114 0.03185
430 0.00585 0.00826 0.00897 0.02969
432 0.00523 0.00759 0.00844 0.02675
434 0.00471 0.00713 0.00698 0.02535
436 0.00446 0.00695 0.00534 0.02364
438 0.00436 0.00641 0.00216 0.02103
440 0.00411 0.00574 0.00184 0.01905
442 0.00344 0.00511 -0.00056 0.01676
444 0.00397 0.00517 -0.00073 0.01598
446 0.00397 0.00458 -0.00243 0.01426
448 0.00376 0.00526 -0.00294 0.01322
450 0.00355 0.00443 -0.00347 0.01145
452 0.00394 0.00386 -0.00358 0.01040
454 0.00312 0.00406 -0.00510 0.00880
456 0.00340 0.00377 -0.00448 0.00870
458 0.00315 0.00340 -0.00577 0.00711
460 0.00294 0.00355 -0.00799 0.00639
462 0.00341 0.00318 -0.00668 0.00615
464 0.00273 0.00321 -0.00651 0.00475
466 0.00310 0.00260 -0.00804 0.00450
468 0.00304 0.00241 -0.00895 0.00423
470 0.00235 0.00199 -0.00877 0.00353
472 0.00272 0.00248 -0.00956 0.00409
474 0.00201 0.00193 -0.00800 0.00269
476 0.00310 0.00338 -0.00347 0.00437
478 0.00290 0.00269 -0.00383 0.00311
480 0.01830 0.00186 -0.00338 0.00307
482 0.00312 0.00259 -0.00394 0.00327
484 0.00349 0.00196 -0.00373 0.00273
486 0.00735 0.00225 -0.00209 0.00266
488 0.00494 0.00181 -0.00421 0.00228
490 0.00318 0.00206 -0.00385 0.00192
492 0.00288 0.00224 -0.00418 0.00169
494 0.01847 0.00201 -0.00357 0.00259
496 0.01158 0.00158 -0.00449 0.00134
498 0.00138 0.00150 -0.00444 0.00155
500 0.00332 0.00148 -0.00445 0.00120
500 0.00332 0.00148 -0.00445 0.00120
(normalized to zero for λ = 500 nm)
NORMALIZED DATA
Wavelength 2.68 5.09 6.34 6.8
230 0.82363 0.80619 0.83116 0.90078
232 1.12148 1.14731 1.04357 1.05567
234 1.15869 1.1521 1.11233 0.99465
236 1.14939 1.10511 1.11656 0.9874
238 1.127 1.02454 1.08064 0.96731
240 1.07179 0.95271 1.02595 0.96211
242 1.00716 0.914 0.95758 0.92683
244 0.9482 0.88078 0.90468 0.88863
246 0.85599 0.79786 0.82121 0.82131
248 0.80622 0.75945 0.7758 0.78294
250 0.75136 0.71904 0.7185 0.73907
252 0.67356 0.6484 0.64846 0.67393
254 0.6414 0.62645 0.61612 0.64988
256 0.6018 0.58862 0.57642 0.60834
258 0.57571 0.56808 0.55097 0.5818
260 0.57083 0.56737 0.54605 0.57345
262 0.57383 0.57326 0.54569 0.5723
264 0.58984 0.59276 0.56077 0.5863
266 0.61712 0.6168 0.58276 0.60625
268 0.6416 0.63959 0.60419 0.62759
270 0.67193 0.67041 0.63018 0.65167
272 0.69899 0.69523 0.65331 0.67497
274 0.72084 0.7151 0.67212 0.69479
276 0.74511 0.73522 0.68979 0.71451
278 0.76543 0.75914 0.71057 0.7336
280 0.78831 0.77698 0.72842 0.75191
282 0.80159 0.78954 0.74087 0.76533
284 0.81345 0.80194 0.75092 0.77699
286 0.82317 0.81028 0.75785 0.78359
288 0.82632 0.81465 0.76175 0.78836
290 0.82936 0.81908 0.76533 0.79201
292 0.84124 0.81764 0.76447 0.79356
294 0.82788 0.81447 0.76271 0.79297
296 0.8245 0.81304 0.75975 0.79145
298 0.82011 0.80933 0.75603 0.78864
300 0.81321 0.80231 0.75053 0.78348
302 0.80377 0.79461 0.74313 0.77619
304 0.79156 0.78377 0.73275 0.76771
306 0.77979 0.7735 0.72283 0.75757
308 0.76462 0.75882 0.71469 0.74552
310 0.74717 0.74309 0.69675 0.73134
312 0.72978 0.72515 0.681 0.71762
314 0.70882 0.70828 0.66314 0.70085
316 0.68517 0.68355 0.64211 0.68009
318 0.66283 0.66362 0.62435 0.66254
320 0.63996 0.64131 0.60496 0.6422
322 0.61289 0.6151 0.57943 0.61817
324 0.58902 0.59317 0.55978 0.59746
326 0.56376 0.56555 0.53723 0.5766
328 0.54128 0.54252 0.51699 0.55465
330 0.52016 0.52095 0.49688 0.53634
332 0.49397 0.4959 0.47377 0.51318
334 0.47215 0.47369 0.45324 0.49338
336 0.45014 0.44956 0.43316 0.47381
338 0.42706 0.42758 0.41054 0.45179
340 0.4061 0.40592 0.39273 0.43487
342 0.38571 0.38512 0.37512 0.41642
344 0.36374 0.36427 0.35545 0.39631
346 0.33582 0.33898 0.33187 0.37313
348 0.31803 0.32043 0.31634 0.35806
350 0.30065 0.30215 0.29816 0.34157
352 0.28409 0.28631 0.28551 0.32955
354 0.2641 0.2676 0.26943 0.31292
356 0.24944 0.25353 0.25521 0.29982
358 0.23456 0.23876 0.24166 0.28821
360 0.21704 0.22049 0.22772 0.27279
362 0.20336 0.20803 0.21635 0.26227
364 0.18904 0.19496 0.20287 0.25103
366 0.1731 0.17918 0.18868 0.23582
368 0.16093 0.16766 0.1783 0.22485
370 0.14678 0.15188 0.1661 0.21253
372 0.13742 0.14199 0.15637 0.20274
374 0.12384 0.1329 0.14502 0.19308
376 0.11388 0.12132 0.13703 0.18411
378 0.1046 0.11188 0.12835 0.17498
380 0.09515 0.10132 0.11847 0.16403
382 0.08482 0.09369 0.10974 0.15535
384 0.07783 0.08413 0.10196 0.14773
386 0.07803 0.07608 0.09433 0.1386
388 0.06237 0.07072 0.08811 0.12994
390 0.05379 0.06266 0.08097 0.12193
392 0.04888 0.05671 0.07464 0.11565
394 0.04163 0.04986 0.0684 0.108
396 0.04088 0.04412 0.06282 0.10209
398 0.03229 0.0391 0.05828 0.09481
400 0.02703 0.03291 0.05285 0.08748
402 0.02299 0.02983 0.04938 0.08146
404 0.01916 0.02571 0.04373 0.07577
406 0.01614 0.02335 0.04025 0.07155
408 0.01357 0.01923 0.03649 0.06544
410 0.02659 0.01716 0.03277 0.06115
412 0.00982 0.01518 0.03029 0.05725
414 0.009 0.01342 0.02756 0.05271
416 0.00637 0.01248 0.0253 0.04941
418 0.00497 0.01096 0.02272 0.04472
420 0.00455 0.00959 0.02186 0.04245
422 0.00379 0.00838 0.01918 0.03805
424 0.00307 0.00824 0.01749 0.03617
426 0.00418 0.00778 0.01675 0.03276
428 0.0036 0.00744 0.01559 0.03065
430 0.00253 0.00678 0.01342 0.02849
432 0.00191 0.00611 0.01289 0.02555
434 0.00139 0.00565 0.01143 0.02415
436 0.00114 0.00547 0.00979 0.02244
438 0.00104 0.00493 0.00661 0.01983
440 0.00079 0.00426 0.00629 0.01785
442 0.00012 0.00363 0.00389 0.01556
444 0.00065 0.00369 0.00372 0.01478
446 0.00065 0.0031 0.00202 0.01306
448 0.00044 0.00378 0.00151 0.01202
450 0.00023 0.00295 0.00098 0.01025
452 0.00062 0.00238 0.00087 0.0092
454 -0.0002 0.00258 -0.00065 0.0076
456 0.00008 0.00229 -0.00003 0.0075
458 -0.00017 0.00192 -0.00132 0.00591
460 -0.00038 0.00207 -0.00354 0.00519
462 0.00009 0.0017 -0.00223 0.00495
464 -0.00059 0.00173 -0.00206 0.00355
466 -0.00022 0.00112 -0.00359 0.0033
468 -0.00028 0.00093 -0.0045 0.00303
470 -0.00097 0.00051 -0.00432 0.00233
472 -0.0006 0.001 -0.00511 0.00289
474 -0.00131 0.00045 -0.00355 0.00149
476 -0.00022 0.0019 0.00098 0.00317
478 -0.00042 0.00121 0.00062 0.00191
480 0.01498 0.00038 0.00107 0.00187
482 -0.0002 0.00111 0.00051 0.00207
484 0.00017 0.00048 0.00072 0.00153
486 0.00403 0.00077 0.00236 0.00146
488 0.00162 0.00033 0.00024 0.00108
490 -0.00014 0.00058 0.0006 0.00072
492 -0.00044 0.00076 0.00027 0.00049
494 0.01515 0.00053 0.00088 0.00139
496 0.00826 0.0001 -0.00004 0.00014
498 -0.00194 0.00002 0.00001 0.00035
500 0 0 0 0
500 0 0 0 0
Compound:
(N-(3-chloro-4-nitrophenyl)-4,5-dihydro-1H-imidazol-2-amine acetate (13a)
0 0 0 0 0 0 0
al wavelengths)
A b s o rb a n c e d iffe re n c e
0
2 3 4 5 6 7 8 9 10
pH
12.24
0.02005
0.54804
0.73220
0.87257
0.90028
0.92045
0.93171
0.91944
0.90777
0.89915 Raw data
0.87221
0.84313 1.20000
0.82001
0.77882
0.73648
1.00000
0.70087
0.67688
Absorbance
0.62408
0.58634 0.80000
0.55379
0.51553
0.48058 0.60000
0.45434
0.42759
0.40851 0.40000
0.39424
0.38256
0.37382
0.20000
0.36385
0.36362
0.37699
0.38266 0.00000
0.39032 250 300 350 400
0.39509
0.41375 -0.20000 Wavelength (nm)
0.41642
0.42843
0.44643
0.46592
0.47592
0.49739
0.51120
0.52641
0.54409
0.56041
0.57607
0.59504
0.61150
0.62258
0.64681
0.66608
0.68668
0.70610
0.71889
0.74601
0.76277
0.78224
0.80430
0.80424
0.82204
0.83790
0.86291
0.88059
0.89748
0.91317
0.92716
0.94483
0.95277
0.96317
0.96901
0.97488
0.97955
0.97955
0.98244
0.97967
0.97284
0.96572
0.95424
0.94085
0.92372
0.90619
0.88704
0.86307
0.83924
0.81065
0.78295
0.75606
0.72280
0.69521
0.66063
0.62867
0.59964
0.56379
0.53240
0.49623
0.46648
0.43362
0.40395
0.37369
0.34569
0.31399
0.29044
0.26487
0.24420
0.22215
0.20085
0.18112
0.16562
0.14999
0.13563
0.12268
0.10929
0.09708
0.08884
0.07969
0.06997
0.06504
0.05699
0.05142
0.04522
0.04061
0.03692
0.03172
0.03011
0.02741
0.02426
0.02154
0.02020
0.01921
0.01713
0.01442
0.01410
0.01401
0.01136
0.01130
0.01121
0.01121
12.24
0.00884
0.53683
0.72099
0.86136
0.88907
0.90924
0.9205
0.90823 Normalized
0.89656
0.88794 1.2
0.861
0.83192
0.8088 1
0.76761
Absorbance
0.72527
0.68966
0.66567 0.8
0.61287
0.57513
0.54258 0.6
0.50432
0.46937
0.44313
0.4
0.41638
0.3973
0.38303
0.37135 0.2
0.36261
0.35264
0.35241
0
0.36578 250 300 350
0.37145
0.37911
0.38388 -0.2 Wavelen
0.40254
0.40521
0.41722
0.43522
0.45471
0.46471
0.48618
0.49999
0.5152
0.53288
0.5492
0.56486
0.58383
0.60029
0.61137
0.6356
0.65487
0.67547
0.69489
0.70768
0.7348
0.75156
0.77103
0.79309
0.79303
0.81083
0.82669
0.8517
0.86938
0.88627
0.90196
0.91595
0.93362
0.94156
0.95196
0.9578
0.96367
0.96834
0.96834
0.97123
0.96846
0.96163
0.95451
0.94303
0.92964
0.91251
0.89498
0.87583
0.85186
0.82803
0.79944
0.77174
0.74485
0.71159
0.684
0.64942
0.61746
0.58843
0.55258
0.52119
0.48502
0.45527
0.42241
0.39274
0.36248
0.33448
0.30278
0.27923
0.25366
0.23299
0.21094
0.18964
0.16991
0.15441
0.13878
0.12442
0.11147
0.09808
0.08587
0.07763
0.06848
0.05876
0.05383
0.04578
0.04021
0.03401
0.0294
0.02571
0.02051
0.0189
0.0162
0.01305
0.01033
0.00899
0.008
0.00592
0.00321
0.00289
0.0028
0.00015
0.00009
0
12.24
-0.81479
-0.58465
-0.4377
-0.28803
-0.23793
-0.16255
-0.08666
-0.03997
0.04057
0.08172
0.10964
0.15836
0.1674
0.16581 Spectral difference
0.14956
0.11883
0.09184 1
0.02303
-0.04199
-0.09902
-0.16761
-0.22962
-0.27771
Absorbance
-0.32873 0.5
-0.36813
-0.40528
-0.43024
-0.45084
-0.47053
-0.47391
-0.46358 0
-0.46979
-0.44877
0
-0.44062
-0.41757
-0.408
-0.38655
-0.35634 -0.5
-0.32508
-0.29991
-0.26099
-0.22979
-0.19362
-0.15229
-0.11363 -1
-0.0751
250 270 290 310 330 350 370 390
-0.02906 Wavelength (nm)
0.01127
318 318 nm
0.04761
0.09432
0.13471
0.1815
0.22274
0.25754
0.30774
0.34546
0.38532
0.42935
0.45721
0.4928
0.52604
0.56761
0.60528
0.63683
0.6674
0.69891
0.73026
0.75252
0.77886
0.79687
0.81689
0.83092
0.8445
0.85735
0.86386
0.86648
0.86969
0.8652
0.85161
0.85014
0.84119
0.82695
0.81023
0.78715
0.76715
0.74471
0.72186
0.69243
0.66786
0.63585
0.59087
0.57861
0.54358
0.51482
0.48005
0.45072
0.41862
0.38967
0.3583
0.33088
0.30025
0.27732
0.25227
0.23185
0.2099
0.18885
0.16979
0.15376
0.13813
0.12398
0.11124
0.09746
0.08607
0.07755
0.06865
0.05914
0.05374
0.04637
0.04043
0.03429
0.03037
0.02631
0.02182
0.01912
0.01662
-0.00193
0.01053
0.00882
0.00397
0.0043
0.00335
0.00333
-0.01235
-0.00811
0.00203
rbance difference vs pH
Ka Determination
6 7 8 9 10 11 12 13
pH
Raw data
Wavelength (nm)
Normalized data
Wavelength (nm)
Spectral difference plot
Column
B
Column
C
Column
D
Column
E
Column
F
Column
G
Column
H
Column
I
Column
J
Column
K
Column
L
Column
G
Column
H
Column
I
Column
J
Column
K
Column
L
Column
M