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This document discusses hydrodynamic gene transfer, a method for introducing DNA into cells by squeezing the cells in microfluidic devices. The process involves flowing cells through narrowing channels so that their membranes stretch and form temporary pores through which DNA can enter without damaging the cells. As the cells return to their original shape, the pores close and trap the DNA inside. The method allows gene transfer without killing cells and works in a variety of cell types. However, it is not feasible for use in humans due to potential effects on cardiac function.
This document discusses hydrodynamic gene transfer, a method for introducing DNA into cells by squeezing the cells in microfluidic devices. The process involves flowing cells through narrowing channels so that their membranes stretch and form temporary pores through which DNA can enter without damaging the cells. As the cells return to their original shape, the pores close and trap the DNA inside. The method allows gene transfer without killing cells and works in a variety of cell types. However, it is not feasible for use in humans due to potential effects on cardiac function.
This document discusses hydrodynamic gene transfer, a method for introducing DNA into cells by squeezing the cells in microfluidic devices. The process involves flowing cells through narrowing channels so that their membranes stretch and form temporary pores through which DNA can enter without damaging the cells. As the cells return to their original shape, the pores close and trap the DNA inside. The method allows gene transfer without killing cells and works in a variety of cell types. However, it is not feasible for use in humans due to potential effects on cardiac function.
BIO 191 Introduction to Genomics and Bioinformatics
HYDROSTATIC GENE TRANSFER C E L L S Q U E E Z E
The cell is allowed to flow in a device, called as the
microfluidic delivery platform. As the cells move through the device, the channel width narrows until a cell finds itself in a tight spot. The cell membrane will change in form in order for the cell to fit into the narrow channel. The forced, rapid change in cell shape creates temporary holes in the membrane without damaging or killing the cell DNA is now then allowed to enter the cell through the pores created from the rapid change in cell shape. Hydrodynamic force generated by a pressurized As the cell returns to its original shape, the pores Injection of large volume of DNA is applied into slowly closes and the DNA that entered it are the blood vessel trapped inside. Capillary endothelium is permeabilized and pores ADVANTAGES DISADVANTAGES are generated - It allows transfer of molecules DNA enters the parenchyma cells through the without damaging or killing the cell (as pores compared to electroporation) With time, the membrane pores close, trapping - it can be used in a wide variety of the DNA inside. animal cells, including those that are fragile to the current transfection ADVANTAGES DISADVANTAGES methods - An effective tool for - not feasible in humans -large molecules are allowed to enter establishing disease in model since it may induce the cell animals irregularity in cardiac - More gene product are function produced as compared to intramuscle injection of References plasmid DNA Johnson, T. (2013, June 3). Researchers Put Squeeze On Cells - The use of animal system as To Deliver. Retrieved from National Institute of compared to bacteria or yeast system is that Biomedical Imaging and Bioengineering: expression of transgene in https://www.nibib.nih.gov/news- rodents avoids the problem events/newsroom/researchers-put-squeeze-cells- of misfolding and improper deliver glycosylation Suda, T., & Dexi, L. (2007). Hydrodynamic Gene Delivery: Its Principles and Applications. Cell Press, 2063-2069.