SD

Chikungunya IgM
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Distinguish Chikungunya from

Dengue virus infection
Rapid, Qualitative detection of Chikungunya IgM antibody
Immunochromatographic assay (ICA) rapid test
Fully covered device to prevent drying
Specimen : Serum, Plasma or Whole blood
Fast results : 10 minutes.
General Information
The word 'Chikungunya' is derived from the Makonde language in southeastern
Tanzania and means "bent down or become contorted," which indicates the
classic posture the patient adopts because of severe joint pain.

Chikungunya fever Approximate Global Distribution of

Chikungunya Virus, by Country, 2008
Chikungunya fever is a viral disease transmitted through
the bite of infected Aedes aegypti and Aedes albopictus
mosquitoes.

Signs and symptoms

An abrupt onset of fever frequently accompanied by joint
pain, muscle pain, headache, nausea, fatigue and rash. http://www.cdc.gov/ncidod/dvbid/Chikungunya/
CH_Globalmap.html

Incubation period Benin, Burundi, Cameroon,

Time from the infected bite to the onset of illness can be C. Africa, Congo DRC, Gabon, Guinea ,
Africa Kenya , Madagascar, Malawi , Nigeria,
3-12 days, but is usually 4-7 days. Senegal, Seychelles, S. Africa, Tanzania,
Uganda, Zimbabwe

Treatment Cambodia, India, Indonesia, Malaysia,

Asia Myanmar, Philippines, Singapore,
Currently specific drug treatment and vaccines are not Sri Lanka, Taiwan, Thailand, Vietnam
available. Europe Italy

Distinguish Chikungunya from Dengue virus infection

Why is important to distinguish Chikungunya from Dengue
Chikungunya and Dengue are among the most difficult diseases to distinguish, especially because
simultaneous co-infection can occur.

Overlap and confusion with dengue fever.

The disease shares some clinical signs with dengue, and can be misdiagnosed in areas where
dengue is common. Hence the number of cases of Chikungunya fever is much higher than
that reported.
Chikungunya fever has similar symptoms but requires different management than dengue.

--> The differential diagnosis of these two infections is essential for clinical
managent and epidemiological study in the tropics.

SD BIOLINE Chikungunya IgM

CHIK: Chikungunya
Detection of Chikungunya IgM in acute phase
Early detection is important in order to stop further transmission and case
management is necessary to prevent severe development of the disease.

Chikungunya Case Definition ( WHO Recommended)

Laboratory criteria: at least one of the following tests in the acute phase
Virus isolation
Presence of viral RNA by RT-PCR
Presence of virus specific IgM antibodies in single serum sample collected in acute or
convalescent stage.
Four-fold rising of IgG titers in samples collected at least three weeks apart

Early detection of IgM

IgG
Inoculation viral RNA IgM

Day-4 to day-2 Day 0 Day 4 to day 7 Day 15

Mosquito Clinical IgM antibody levels are highest three to five

Bite symptoms weeks after the onset of illness and persist for
about two months.

Excellent performance
Internal Evaluation
Sensitivity : 97.1% (vs. ELISA)
Specificity : 98.9% (vs. ELISA)

Clinical study
According to the research performed in southern Thailand(Chikungunya outbreak, 2008), SD BIOLINE
Chikungunya IgM shows equivalent performance when compared to RT-PCR for collected CHIKV
positive samples after onset of fever. RT-PCR for CHIKV was positive in every serum obtained from
patients within the first three days after the onset of fever whereas the IgM antibody may become
detectable after 3 days of the onset of fever. In this research, author emphasizes that serological or
molecular methods are required to arrive at a definite diagnosis of either infection. RT-PCR and serology to
detect IgM antibodies or paired sera for IgG against CHIKV ought to be performed in all patients with
presumed hemorrhagic fever to detect outbreaks of CHIKV early. This precaution would help control global
epidemics of this virus.
Specification
Specimen : Serum, Plasma (50l) , Whole blood (100l)
Assay Time : 10 minutes
Detection : Chikungunya IgM antibody
Sensitivity : 97.1%, Specificity : 98.9% (vs. ELISA)
Component : Test device/ Assay diluent/ Disposable droppers

Test Procedure
Serum or Plasma Negative

1 drop 1 drop
(50l)

10 min. Positive

Whole Blood
Invalid
2 drops
(100l)

Reference
http://www.searo.who.int/LinkFiles/Chikungunya_FactSheet_Chikungunyafever.pdf
http://www.who.int/mediacentre/factsheets/fs327/en/
http://www.searo.who.int/LinkFiles/Chikungunya_Draft_Prevention&Control_CHIK_25_Nov.pdf
http://ecdc.europa.eu/pdf/071030CHK_mission_ITA.pdf
http://www.cdc.gov/ncidod/dvbid/Chikungunya/CH_GlobalMap.html

Ordering Information
Rapid Test
Cat. No Product Specimen Type Pack size Storage

46FK10 Chikungunya IgM Serum/Plasma/ Device 1T x 25/Kit 24 months

Whole blood 1~30 C

ELISA available
Cat. No Product Specimen Type Pack size Storage
Chikungunya IgM 12 months
46EK10 Serum Well 96wells/Kit
ELISA 2~8 C
Cat. No. LEF46EN
Rev. 1310-00

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