Republic of the Philippines

Department of Education
Region VII, Central Visayas
Division of Carcar City
Ocaña National High School
Ocaña, Carcar City, Cebu
A.Y 2019-2020

The Antimicrobial Ability of White Lead Tree (Leucaena glauca L.) Alkaloid Seed Extracts

Against Water Coliform Found in the Water Reservoir of Biasong,

Poblacion 1, Carcar City, Cebu

(Proposal)

Theryze Anne D. Baring

Researcher

Mrs. Farrah G. Madrio

Research Adviser
CHAPTER I

A. BACKGROUND OF THE STUDY

Public water systems must deliver safe and reliable potable water to their customers 24

hours a day, 365 days a year. If the source of water becomes contaminated, consumers can get

seriously ill which results to another problem. Fortunately, public water systems take many

alterations and few extra steps to make sure water travelling through the pipes is safe. One of the

most important steps is regular testing for coliform bacteria.

Coliform microorganism are gift within the largely all over in the setting and feces of

all homoeothermic animals and humans. Coliform microorganisms are unlikely to cause ill

health. However, their presence in drink indicates that disease-causing organisms

(pathogens) may be within the water system. Most pathogens that may contaminate

water provide return from the body waste of humans or animals.

Testing water for all doable pathogens is advanced, long, and pricy. It is

straightforward and cheap to check for coliform bacterium. Water systems hunt for the supply of

contamination and restore safe water, if testing detects coliform bacterium in an exceedingly

water sample.

When coliform microorganisms are found, water systems investigate to search

out however the contamination got into the water. They collect extra water samples and

infrequently examine the complete system. Collecting extra samples helps verify whether or

not an actual downside exists. If the work detects bacteria in any of the extra samples, the initial

findings are “confirmed.”

Water is also treated otherwise in numerous communities looking on the standard of the

water that enters the treatment plant. Typically, surface water needs a lot of treatment and
filtration than spring water as a result of lakes, rivers, and streams contain a lot of sediment and

pollutants and are more seemingly to be contaminated than spring water.

In this regards, the researcher will conducted a study to make some innovations using

White Lead Tree (Leucaena galuca L.) Alkaloid seed extracts common called “ipil-ipil” within

the Philippines, to treat Coliform contaminated cistern reservoirs in Biasong, Poblacion 1, Carcar

City,Cebu.

The current study will study the effectiveness of different concentrations of the White

Lead Tree (Leucaena galuca L.) Alkaloid seed extracts towards the treatment of the water tank

reservoirs in different ratio of water to extract and days it will be cured.

 B. STATEMENT OF THE PROBLEM

This study aims to test the antimicrobial ability of White Lead Tree (Leucaena galuca L.)

Alkaloid seed extracts towards the Water Coliform present in the water tank reservoir of

Biasong, Poblacion1, Carcar City Cebu. (2 hours observation)

Specifically, this will answer the following questions:

a.) What concentration of alkaloid seed extract works best at different ratios of

contaminated water?

a.1.) 2 mL: 10 mL

a.2.) 2 mL: 5 mL

a.3.) 2mL: 2mL

a.4.) 5mL: 5mL

a.5) 5mL: 10 mL

a.6) 5mL: 15mL

a.7)7mL: 7mL

a.8)7mL: 10mL

a.9)7mL: 14mL

a.10) 1mL: 2mL

b.) What is the zone of inhibition of the coliform water sample after is has been applied

with White Lead Tree (Leucaena glauca L.) Alkaloid See Extract of the following

ratios? (extract is to water)

b.1.) 2 mL: 10 mL

b.2.) 2 mL: 5 mL

b.3.) 2mL: 2mL

b.4.) 5mL: 5mL

b.5) 5mL: 10 mL

b.6) 5mL: 15mL

b.7)7mL: 7mL

b.8)7mL: 10mL

b.9)7mL: 14mL

b.10) 1mL: 2mL

c.) Is there a significant difference between the microbial ability of different

concentrations of White Lead Tree (Leucaena galuca L.) Alkaloid seed extracts

towards the zone of inhibition of Coliform contaminated water sample present in the

water tank reservoir of Biasong, Poblacion1, Carcar City, Cebu?

 C. STATEMENT OF THE HYPOTHESIS

NULL HYPOTHESIS

There is no significant difference between the microbial ability of different

concentrations of White Lead Tree (Leucaena galuca L.) Alkaloid seed extracts towards the zone

of inhibition of Coliform contaminated water sample present in the water tank reservoir of

Biasong, Poblacion1, Carcar City,Cebu.

ALTERNATIVE HYPOTHESIS

There is a significant difference between the microbial ability of different concentrations

of White Lead Tree (Leucaena galuca L.) Alkaloid seed extracts towards the zone of inhibition

of Coliform contaminated water sample present in the water tank reservoir of Biasong,

Poblacion1, Carcar City,Cebu.

 D. SIGNIFICANCE OF THE STUDY

Microorganisms are found in nearly each natural component on the earth. The lives of

humans, plants and animals are all as such joined to the microbes that regularly recycle key

nutrients like Carbon and chemical element, degrade organic matter and form our day to day

existence.

The results will be a great benefit to the following:

Community- those who are living near the reservoir or those areas where the water from the

reservoir travels to, their supply of water will be of clean and essential, which in this case will

deficit the number of sanitary issues and also maintains healthy water for their supply.

Children- kids and young organisms take into account animals; have low immune system which

is a part risky when their water supply is contaminated with coliform. This study will help

diminish these coliforms to maintain a clean and potable water healthy enough for drinking.

Students- this study will add information to students. The data that will be gathered can help

students have an area of understanding regarding a water’s safety and help them learn that “ipil-

ipil” is not just a plant that is rampant at any place but can also be a very helpful substitute for

chlorine.

Resort Owners, Sanitary Office, and Government- this study will redound to the betterment

of a clean environment without using harmful substitutes for cleaning water systems like tanks,

pools, and etc instead, they will be aware for the potential organic help of this study.
 E. SCOPES AND LIMITATIONS

This study will focus the effect of the White Lead Tree (Leucaena galuca L) Alkaloid

seed extract towards the coliform contaminated water of Sitio Biasong, Poblacion 1, Carcar City,

Cebu only. This study will be focusing on the microbial ability of the said plant in different

concentrations. This is will be limited only to the said purpose of the study. No locals and

animals will be harmed of this experiment. Only one location and water tank will be used as

source of water sample and that is from Sitio Biasong, Poblacion 1, Carcar City, Cebu. This

research is all about the potential use of “ipil-ipil” as a substitute for bleach and other harmful

chemicals to clean contaminated water and it will be stated here about the results and how it will

affect the environment including the community.

 F. DEFINITION OF TERMS

To further understand the study made by the researcher, the following terms are defined

as to how they are used in the study:

Coliforms- are a broad class of bacteria found in our environment, including the feces of man

and other warm-blooded animals. The presence of coliform bacteria in drinking water may

indicate a possible presence of harmful, disease-causing organisms. (Public Health Benton-

Franklin Health District, March 2002)

Microbiology- is the study of microorganisms, which are unicellular or cell-cluster microscopic

organisms. This includes eukaryotes such as fungi and protists and prokaryotes such as bacteria

and certain algae. Microbiology subdivided into divisions including bacteriology, virology,

mycology, parasitology and others. (Caister Academic Press, January 2019)

Reservoir- Reservoirs tanks are used to feed bulk fluids to a variety of dispensing valves. A

range of sizes are available including bottom ported for medium to high viscosity liquids and top

ported for low to medium viscosities. (Fisnar, December 2018)

White Lead Tree (Leucaena glauca) - The flower heads are round, white, 3/4 inch (1.9 cm) in

diameter puffballs of numerous white stamens with hairy, pale yellow anthers. The flowers are

followed by clusters of linear, up to 7 inch (18 cm) long, flat, thin, drooping, green drying to

papery brown bean pods with a bead necklace-like row of seed bumps. The seeds are flat, oval,

and a rich glossy brown color. The leaves are bipinnately compound with small, green, narrowly

oblong, point-tipped leaflets. The branches have brown, corky pores and are thorn less, hairy

when young, and brown and hairless when old. (Hawaiian Plants and Tropical Flowers, February

2019)
CHAPTER III

RESEARCH DESIGN

The research design that will be used in this study is experimental design. The researcher

will experiment with the White Lead Alkaloid Seed Extract towards the water sample that will

collected from the water tank reservoir located in Sitio Biasong, Poblacion 1, Carcar Ciy, Cebu.

METHODOLOGY

A. Gathering of Materials

The researcher will gather 100mL pure Alkaloid Seed Extract from White Lead Tree,

1000mL beaker, electric weighing scale, stirrer, 100mL hexane, bleach, hot plate, 200mL worth

of water sample, 10 3m petri film, chromatography paper, test tubes, marker, 100mL Isopropyl

Alcohol (70% concentration), test tube racks, filter papers, mortar and pestle, and

chromatography papers.

For Proper Protective Equipment, the researcher will prepare a mask, pair of gloves, lab

coat, sealed bags and lab goggles.

B. Collection of Materials

The researcher will first sanitize the equipments using 10% bleach and 90% water in the

hot plate. Then, the researcher will collect a water sample using the beaker at Sitio Biasong,

Poblacion 1, Carcar City, Cebu. After, the researcher will collect about 100 White Lead Tree at

Biasong, Carcar City, Cebu.

 C. Procedure and Application

The researcher will pound the seeds until it will yield an extract with the amount of

100mL. Then, the researcher, using the different concentrations, will get the ratio of the amount

of water to the amount of extract using the test tube. The researcher will wait for 2 hours and

using the dropper will get 1mL of concentration and put it in the 3m petri film. The observation

period will last 36 hours. Then the researcher will record her observation.

Concentration 2:10 2:5 2:2 5:8 5:2 5:10 7:10 7:3 7:10 1:2

(mL) (mL) (mL) (mL) (mL) (mL) (mL) (mL) (mL) (mL)

TRIAL 1

TRIAL

TRIAL

TRIAL

Table 1: Extractis to Water Ration (Zone of Inhibition) *results

 Statistical Treatment

The result from the reaction of the Coliform from the contaminated water source to the

different concentrations of applied White Lead Tree (Leucaena galuca L) Alkaloid seed extract

will be statistically analyzed. To know if there is a significant difference between the microbial

ability of different concentrations of White Lead Tree (Leucaena galuca L.) Alkaloid seed

extracts towards the zone of inhibition of Coliform contaminated water sample present in the

water tank reservoir of Biasong, Poblacion1, Carcar City,Cebu, will be utilized with f-test of one

way ANOVA at 0.05 level of confidence.

Table 2: Extractis to Water Ration (Zone of Inhibition) *data for Hypothesis Testing

Concentration 2:10 2:5 2:2 5:8 5:2 5:10 7:10 7:3 7:10 1:2

(mL) (mL) (mL) (mL) (mL) (mL) (mL) (mL) (mL) (mL)

TRIAL 1

TRIAL

TRIAL

TRIAL

4
Choice of Statistics: F-test of One way ANOVA

SST:

SSW:

SSB:

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