STBP2203

Sem 2 2018/2019

PROFORMA KURSUS

KOD KURSUS : STBP2203

NAMA KURSUS : Amali Biokimia I / Biochemistry Practical I
JAM KREDIT : 3
SINOPSIS :
Eksperimen yang dilakukan di dalam kursus ini merangkumi kaedah asas yang penting dalam teknik-
teknik biokimia seperti pengemparan biomolekul, pengukuran pH, penyediaan penimbal serta
pengukuran kuantitatif sebatian biokimia serta analisis kinetik enzim menggunakan kaedah
spektrofotometri. Prinsip biokimia dalam kaedah pengasingan makromolekul seperti kromatografi
dan elektroforesis juga dibincangkan. Kuliah dan tutorial post-mortem diberikan untuk
memantapkan prinsip teori amali yang dijalankan.

Experiments carried out in this course encompasses basic techniques in biochemistry such as in
centrifugation of biomolecules, pH measurements, buffer preparation as well as quantitative
determinations of biomolecules; and kinetic analysis of enzymes using spectrophotometry.
Biochemical principles of separation techniques for biomolecules including chromatography and
electrophoresis are also discussed. Lectures and post-mortem tutorials are given to strengthen the
theoretical principles of the experiments carried out.

HASIL PEMBELAJARAN KURSUS

HPK 1 Berkebolehan untuk menganalisis prinsip pH dan penimbalan, spektrofotometri,

kromatografi, pengasaian dan kinetik enzim
(Ability to analyse principles of pH and buffering, spectrophotometry,
chromatography, electrophoresis, enzyme assay and kinetics)
HPK 2 Berkebolehan mendemonstrasi kemahiran untuk analisis pH dan penimbal,
pengasaian enzim, penentuan dan pemfraksian protein serta bekerja secara
berkumpulan
(Ability to demonstrate skills in analysis of pH and buffer, enzyme assays, protein
analysis and fractionation; and work as a team)
HPK 3 Berkebolehan untuk menilai hitungan biokimia untuk pH dan penimbal,
pemfraksian protein, pengasaian dan kinetik enzim
(Ability to evaluate biochemical calculations on pH and buffers, protein
fractionation, enzyme assays and kinetics)
HPK 4 Berupaya untuk menganalisis dan menginterpretasi data eksperimen
(Ability to analyse and interpret experimental data)
HPK 5 Berupaya untuk menghuraikan hasil eksperimen secara tepat dalam bentuk
laporan amali
(Ability to elaborate experimental results accurately in the form of a laboratory
report)

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STBP2203 Sem 2 2018/2019

RUJUKAN
1. Boyer, R.F. 2011. Biochemistry Laboratory: Modern Theories and Techniques. 2nd edition. New
Jersey, USA: Prentice Hall.
2. Campbell, M. K. & Farrell, S. O. 2014. Biochemistry. 8th Edition. USA: Brooks/Cole Cengage
Learning.
3. Damodaran, G.K. 2010. Practical Biochemistry. New Delhi, India: Jaypee Brothers, Medical
Publishers
4. Farell, S.O. & Taylor, L.E. 2006. Experiment In Biochemistry: A Hands-On Approach. 2nd Edition.
Belmont, CA USA: Thomas Brooks/Cole.
5. Wilson, K. & Walker J.M. 2010. Principals and Technique of Practical Biochemistry and Molecular
Biology. 7th Edition. UK: Cambridge University Press

KAEDAH PENILAIAN
Laporan amali : 30%
Ujian amali : 50%
Kuiz : 10%
Buku Log : 10%


TENAGA PENGAJAR
Dr. Muhammad Ashraf Shahidan (MAS) : mashraf@ukm.edu.my (Course coordinator)
Dr. Babul Airianah Othman (BAO) : airianah@ukm.edu.my
Dr. Shazilah Kamaruddin (SK) : shazilah@ukm.edu.my
Dr. Shevin Rizal Feroz (SRF) : shevin@ukm.edu.my


PEGAWAI AMALI
En. Kamarul Hata Abu (Pegawai Sains)
En. Abdul Razak Abdullah (Penolong Pegawai Sains)
Pn. Norhabsah Abdul Jalil (Penolong Pegawai Sains)
Pn. Nurulizwanie Sanusi (Penolong Pegawai Sains)
Pn. Zaiton Othman (Penolong Pegawai Sains)
Cik Aninah Mohamad Hanapiah (Pembantu Makmal)

KALENDER AKADEMIK

Minggu Pembelajaran (1 – 7) : 18 Februari 2019 – 5 April 2019
Cuti Pertengahan Semester : 6 April 2019 – 14 April 2019
Minggu Pembelajaran (8 – 14) : 15 April 2019 – 31 Mei 2019
Minggu Ulang kaji : 1 Jun 2019 – 9 Jun 2019

CUTI UMUM

1 Mei 2019 : Hari Pekerja (Rabu)
20 Mei 2019 : Wesak (Isnin)
22 Mei 2019 : Nuzul Al-Quran (Rabu)

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STBP2203 Sem 2 2018/2019

PERINCIAN KULIAH MENGIKUT TAJUK

Lectures: Wednesday (10 – 11 am, DKG 130 BB) and Friday (10 – 11 am, DKG 130 BB)

Topics Hours Date (2019) Lecturer

1 Course overview: Introduction 1 20 Feb MAS
2 Centrifugation and cell fractionation
• Principles of centrifugation and isolation of 1 22 Feb MAS
sub-cellular components
Post-mortem 1 27 Feb
3 Spectrophotometry
• Principles of colorimetry and protein 1 1 Mac MAS
determination
Post-mortem 1 6 Mac
4 pH and buffers
• Properties and preparation of buffer 1 8 Mac BAO
solutions
• Post-mortem 1 13 Mac
5 Chromatography
• Principles and methods of gel filtration and 1 15 Mac BAO
ion exchange chromatography
• Post-mortem 1 20 Mac
6 Electrophoresis
• Principles of electrophoresis 1 22 Mac SRF
• Polyacrylamide gel electrophoresis: native
and SDS-PAGE
• Post-mortem 1 27 Mac
Practical Exam I 1 2 April MAS/BAO
7 Enzyme kinetics
• Determination of kinetic parameters 1 5 April SK
• Post-mortem I 1 17 April
• Post-mortem II 1 24 April
8 Protein purification
• Purification strategy and evaluation of purity 1 26 April SRF
and recovery
• Post-mortem 1 3 Mei
Practical Exam II 1 7 Mei SK/SRF
Total hours
Lectures 16
Practical exams 2

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STBP2203 Sem 2 2018/2019

PERINCIAN AMALI MENGIKUT TAJUK

Practical: Tuesday, 2 – 5 pm, Lab 1137/1146

No. Practical Hours Date

1. Purification and characterization of α-lactalbumin from
milk
a) Preparation of whey 3 26 Feb
b) Protein determination by Bradford Assay 3 5 Mac
2. pH and buffer solution 3 12 Mac
3. Purification and characterization of α-lactalbumin from
milk
c) Gel filtration chromatography 3 19 Mac
d) α-lactalbumin electrophoresis and UV spectrum 3 26 Mac
analysis
3. Enzyme kinetics studies of phytase from Aspergillus
ficuum
a) Inorganic phosphate standard curve, protein
standard curve, and determination of initial 3 16 April
velocity
b) Determination of effects of enzyme
concentration, pH and substrate concentrations 3 23 April
on enzyme activity
4. In silico protein purification
- Simulation of fractionation and purification of 3 30 April
protein samples by various procedures
Total hours 24

DATE OF LAB REPORT SUBMISSION

Practical Title Date of submission Lecturer

Set A Set B
2A Preparation of whey 11 Mac 12 Mac MAS
2B Protein determination by Bradford Assay 11 Mac 12 Mac MAS
1 pH and buffer solution 18 Mac 19 Mac BAO
2C Gel filtration chromatography 25 Mac 26 Mac BAO
2D α-lactalbumin electrophoresis and UV 1 April 2 April SRF
spectrum analysis
3 Enzyme kinetics studies of phytase from 29 April 30 April SK
Aspergillus ficuum
4 In silico protein purification 6 Mei 7 Mei SRF
*Note: Two (2) students per lab report, except for protein purification (individual report)