PLANT TRANSPIRATION

By :
Aulya Ammara K B1B017005
Ramadi Habib F B1B017006
Pratiwi Kusuma K B1B017007
Thalitha Nindika N B1B017009
Group : VII
Subgroup :2
Assistant : Maria Pricilia Gita

PRACTICAL REPORT OF PLANT PHYSIOLOGY I

MINISTRY OF RESEARCH, TECHNOLOGY AND HIGHER EDUCATION

JENDERAL SOEDIRMAN UNIVERSITY
FACULTY OF BIOLOGY
PURWOKERTO
2018
 I. INTRODUCTION

A. Background

Transpiration is the loss of water from the plant body in the form of water vapor
through stomata, cuticles or lenticels. There are two types of transpiration, namely cuticle
transpiration is evaporation of water that occurs directly through the epidermal cuticle;
and stomatal transpiration, which in this case water loss takes place through stomata. The
leaf cuticle is relatively non-translucent, and in most types of plant cuticle transpiration
is only 10 percent or less of the amount of water lost through the leaves. Therefore, most
of the water lost through the leaves (Wilkins, 1989).
The speed of transpiration rate is varies depending on the type of plant. Various
ways to measure the amount of transpiration, for example by using weighing methods. A
fresh leaf or even all the plants and pots are weighed. After a certain amount of time, it is
weighed again. The difference in weight between the two weighs is the indicator number
of transpiration. The weighing method can also be directed to the water that is released,
that is by capturing the water vapor that is released with the hygroscopic substance that
has known its weight. Weight gain is a sign of the amount of transpiration
(Tjitrosoepomo, 1998). In addition, to measure the transpiration rate that occurs in the
leaves indirectly, the absorption speed can be measured using the photometry method
using a photometer. In addition to the photometric method, there are many other methods
that can be used including the gravimetric method (weighing) or the lysimeter method
(pot method); cobalt paper method (Cobalt Chloride paper); and semi-quantitative
methods (Ashari, 1995).
The difference between transpiration and evaporation is that in the transpiration
there is a modified physiological or physical process, regulating stomatal openings,
regulating some kinds of pressure, occurring in living tissue and the surface of wet cells,
whereas in evaporation there is a pure physical process, not regulated by stomatal
openings, not regulated by pressure, not limited to living tissue and the surface running it
dry. Most of the water absorbed by plants is transposed. For example: corn plants, from
100% absorbed water: 0.09% to compose the body, 0.01% for reagents, 98.9% to be
transposed (Fitter, 1991).
B. Purpose

The objectives of this laboratory activity are:

1. To know the speed of transpiration rate
2. To know the amount of water vapored, in unit of leaf area within a certain time
 II. MATERIAL AND METHODS

A. Material

The materials that used in this practice are plant twig and water.
The tools that used in this practice are Erlenmeyer/ bottle thst is given a cover
or Styrofoam, analytic scale, scissors and gardening scissors, ruler, and label.

B. Methods

1. Measurement of transpiration
a. All tools and materials are prepared,
b. Water is poured into an Erlenmeyer or bottle until ¾ of the bottle,
c. Plant twig with leaves is placed in the Erlenmeyer or bottle that has been filled
with water,
d. The opening of the bottle is covered with cotton and wrapper,
e. Initial weight of bottle with plant twig and water are weighed,
f. Each plant is placed in different places, which are: (1) area in front of fan , (2)
in bright light, (3) in the dark,
g. Each plant weighed every 60 minutes and the difference in weight is observed.
2. Measurement of leaf area
a. All the leaves attached to the twig are removed and weighed,
b. Cut ten leaves pattern with a measurement of 1 cm2 is made and weighed,
c. Determine the leaf area of the leaves attached to the twig.
 III. RESULT AND DISCUSSION

A. Result
B. Discussion
 IV. CONCLUSION

Based on the result can be concluded that :

 REFERENCES

Ashari, S. 1995. Hortikultura Aspek Budidaya. Jakarta: UI Press.

Fitter, A. Hay, R. 1991. Fisiologi Lingkungan Tanaman. Yogyakarta: Gadjah Mada

University.

Tjitrosoepomo, H. 1998. Botani Umum. Yogyakarta: UGM Press.

Wilkins, M. 1989. Fisologi Tanaman. Jakarta: Bumi Aksara.