Beruflich Dokumente
Kultur Dokumente
(Estted. under the Pan jab University Act VII of 1947-enacted by the Govt. of India)
FACULTY OF SCIENCE
SYLLABI
FOR
BIOTECHNOLOGY
(ELECTIVE)
Semester System
1
BIOTECHNOLOGY (ELECTIVE) EXAMINATIONS 2018-2019
st st
B.Sc. 1 Year (1 Semester)
Code
st nd
B.Sc. 1 Year (2 Semester)
Code
2
B.Sc. 2nd Year (3rd Semester)
Code
S.No. Course/Paper Theory Practical
Course No. Marks Course No. Marks
Introduction to Genetic
1. Engineering and BIOT-Elect-Sem-III-T 75 BIOT-Elect-Sem-III-P 25
Immunotechnology
M. Marks: 100 Time: 3 Hours
Code
S.No. Course/Paper Theory Practical
Course No. Marks Course No. Marks
Fundamentals of
1. Molecular Biology BIOT-Elect-Sem-IV-T 75 BIOT-Elect-Sem-IV-/P 25
and Genetics
M. Marks: 100 Time: 3 Hours
Code
S.No. Course/Paper Theory Practical
Course No. Marks Course No. Marks
Plant & Animal
1. BIOT-Elect-Sem-V-T 75 BIOT-Elect-Sem-V-/P 25
Biotechnology
M. Marks: 100 Time: 3 Hours
Code
S.No. Course/Paper Theory Practical
Course No. Marks Course No. Marks
Environment &
1. Fermentation BIOT-Elect-Sem-VI-T 75 BIOT-Elect-Sem-VI-/P 25
Biotechnology
M. Marks: 100 Time: 3 Hours
3
BIOTECHNOLOGY (ELECTIVE)EXAMINATIONS 2018-2019
st st
B.Sc. 1 Year (1 Semester)
Code
• Set nine questions in all from three sections. All questions carry equal
marks.
O Section A will cover Unit I & II
• Set 2 questions from each unit out of which any 2 are to be attempted
O Section – B will cover Unit – III & IV
• Set 2 questions from each unit out of which any 2 are to be attempted
Objective:
4
UNIT- I
UNIT- II
UNIT –III
UNIT- IV
5
devices, memory and application.
Reference Books:
6
Paper: Introduction to Biotechnology (Practical) Theory: 22 Marks
7
st nd
B.Sc. 1 Year (2 Semester)
Code
• Set nine questions in all from three sections. All questions carry equal
marks.
O Section A will cover Unit I & II
• Set 2 questions from each unit out of which any 2 are to be attempted
O Section – B will cover Unit – III & IV
• Set 2 questions from each unit out of which any 2 are to be attempted
Objective:
UNIT- I
Microbiology
History of Microbiology.
8
Classification of Microbes, current methods of microbial identification,
characteristic features of Eubacteria, Archaebacteria, Fungi, Algae,
Protozoa and Viruses.
Nutritional classification of micro-organisms: microbes in extreme
environments the thermophiles and alkalophiles.
Nature of the microbial cell surface: Gram positive and gram negative
bacteria, flagella, pili, cell inclusions, endospore, growth curve,
microbial metabolism and growth, synchronized and continuous
growth.
Products from microorganisms: Primary and Secondary metabolites
and their commercial uses.
Unit-II
Basic Biochemistry
Suitability of organic compounds for generation of structure, storage of
energy & information.
Structure & Functions of Biomolecules: Carbohydrates – Classification,
characteristics, chemical reactions and functions of monosaccharides,
disaccharides, polysaccharides (structural and storage). Epimers, isomers,
anomers, pyranose and furanose forms, Reducing and non-reducing sugars.
Lipids: Classification and function of lipids.
Unit-IV
Electron Microcopy: SEM, TEM, STEM and their applications in biology,
Preparation of samples for electron microscopy.
9
Spectrometry: Principles & applications of UV, VIS spectrometry, IR and
Raman spectroscopy.
Hybridization Techniques: Southern blotting, Northern Blotting, Western
blotting.
Radioisotope Techniques: Proportional and scintillation counter and
autoradiography; principles and applications.
10
B.Sc. 2nd Year, 3rd Semester
Code
S.No. Course/Paper Theory Practical
Course No. Marks Course No. Marks
Introduction to Genetic
1. Engineering and BIOT-Elect-Sem-III-T 75 BIOT-Elect-Sem-III-P 25
Immunotechnology
M. Marks: 100 Time: 3 Hours
Objective:
The objective of this paper is to introduce the fundamental concepts of genetic engineering
and its application in producing recombinant proteins and other beneficial products of
commercial values in various microbial systems including E.coli, yeast, fungi etc. This course
will also enhance the students’ understanding of immune response in animals / humans and
pathways which provide resistance against pathogens. Further, the students will learn about
the immune cells, the MHC complex and the role of antigens and antibodies.
UNIT-I
Introduction: History and scope of recombinant DNA technology. Gene cloning and need to
clone a gene. DNA Modifying enzyme, Restriction endonucleases, exonucleses, ligases,
polymerase, kinase, alkaline phosphatase, topoisomerases etc.
Purification of DNA from bacterial, plant and animal cells.
Cloning and expression vectors: Cloning vectors for E.coli and yeast, bacteriophages and
cosmids.
UNIT-II
DNA cloning strategies : Preparation of genomic and cDNA libraries, transformation and
transfection, electroporation, screening of gene library and selection of clone.
Introduction to PCR and its applications
11
UNIT-III
Immunotechnology
Basic Immunology: Types of immunity – innate, acquired, active and passive.
Cells and tissues of immune system: Lymphoid cells, mononuclear cells, lymphoid organs.
Antigen:- Immunogenicity, chemical composition, immunogen dosage, Haptens, adjuvants.
Antibody structure, function and types-Antibody diversity, Ig Domains.
Ag-Ab interactions – Cross reactions, precipitation and agglutination.
Immunological Techniques: Immunodiffusion, Immunoelectrophoresis.
UNIT-IV
References:
1. Immunology by J. Kuby, 6th Edition (2007) W.H. Freeman.
2. Essentials of Immunology by I.Roitt 12th Edition (2011)
3. Immunology : An Introduction – I.R. Tizard 9th Edition (2012), Elsevier
4. A handbook of practical Immunology Eds. G.P. Talwar and S.K.Gupta, 2nd Edition CBS
Publishers & Distributors Pvt. Ltd., New Delhi, India, 2009
5. Principles of Genes manipulation by R.W. Old, Twyman and S.B. Primrose, (2002) 6th
Edition. Blackwell Science.
6. Gene cloning and DNA Analysis –An Introduction by T.A. Brown 6th Edition (2007),
Wiley-Blackwell
7. Gene VIII, B. Lewin, (2004)
8. Lewin’s Genes X by Jocelyn E. Krebs, Eliot.E Granstein, (2011). Jones and Bartlett
publishers
9. Genetics: From Genes to Genomes by Hartwell et al. 4th Edition (2010)
10. Fundamentals of Genetic Engineering (Vol.12) by R.H. Rehm and G. Reed.
12
B.Sc. 2nd Year, 4th Semester
Code
S.No. Course/Paper Theory Practical
Course No. Marks Course No. Marks
Fundamentals of
1. Molecular Biology BIOT-Elect-Sem-IV-T 75 BIOT-Elect-Sem-IV-/P 25
and Genetics
M. Marks: 100 Time: 3 Hours
• Set nine questions in all from three sections. All questions carry equal marks.
• Section A will cover Unit I & II
Set 2 questions from each unit out of which any 2 are to be attempted
• Section – B will cover Unit – III & IV
Set 2 questions from each unit out of which any 2 are to be attempted
• Section – C will be compulsory and will have 7 – 10 short answer type (not objective type)
questions covering the whole syllabus.
Objective:
In this course, the students will learn about the genetic makeup of prokaryotes and
eukaryotes, the DNA structure, its replication, transcription, translation and the mode of
developing recombinants. Additionally, this course will cover the basics of mutations
(induced and spontaneous) and their importance in studying gene function.
UNIT-I
Molecular basis of life
Structure of DNA, evidences, properties, types.
DNA replication both prokaryotes and eukaryotes, DNA polymerases, DNA recombination
molecular mechanism prokaryotic and eukaryotic,
UNIT-II
Structure of Prokaryotic and eukaryotic genes.
UNIT-III
Translation :Initiation, Elongation and Termination in prokaryotes and Eukaryotes
13
Post translation modifications : Acetylation, glycosylation and phosphorylation
UNIT-IV
Mendelian Laws of inheritance
Chromosomal theory of inheritance
Chromosome structure and organization, chromosomal banding
Structural and numerical aberrations in chromosomes
Gene mapping – linkage
Population genetics
Mutations:- Types, chemical and physical mutagens, induced mutations in plants, animal and
microbes and their importance.
Insertion elements and Transposons.
References:
1. Encyclopedia of Molecular Biology (1994) S.J. Kendrew.
2. Molecular Cell Biology by Lodish et al (2004) Freeman & Co.
3. Molecular Biology of the genes by Watson et al, 5th Ed., (2006) Pearson Edu.
4. Biochemistry by Voet & Voet 2004 John Wiley & Sons Ltd., New York.
5. Genetics A molecular Approach (Peter – J. Russel) 2006
6. Genetics by Peter Snustand and Michael J. Simmons 6th edition (2012)
7. Genetics by Strickberger M.W, 3rd edition
M. Marks: 25
14
B.Sc. 3rd Year, 5th Semester, Biotechnology (Elective)
Code
S.No. Course/Paper Theory Practical
Course No. Marks Course No. Marks
Plant and Animal
1. BIOT-Elect-Sem-V-T 75 BIOT-Elect-Sem-V-/P 25
Biotechnology
M. Marks: 100 Time: 3 Hours
Set nine questions in all in three sections. All questions carry equal marks.
Section A will cover Unit I & II
Set 2 questions from each unit and any 2 is to be attempted
Section – C will be compulsory and will have 7 – 10 short answer type (not objective
type) questions covering the whole syllabus.
Objective:
Since Biotechnology deals with improvement of plants and animals and culturing of
specific cells/ cell lines in laboratory. These require in depth knowledge of various techniques
of culturing of plant and animal cells, formulation of media, respective vectors and their uses
in transformation and probable contaminations and remedies to overcome them.
PLANT BIOTECHNOLOGY
UNIT-I
Introduction to in vitro methods : Micropropagation ovule & ovary culture, embryo culture,
endosperm culture, somatic embryogenesis & organogenesis, somaclonal & gametoclonal
variations, haploids & their applications.
Protoplast isolation, methods, testing their viability & regeneration, various methods of
fusion: somatic hybridization & their applications.
15
UNIT-II
Direct and indirect transformation of plants. Tumor formation in plant using Agrobacterium
tumefaciens. Mechanism of T-DNA transfer to plants, plasmid vectors for plant
transformation. Genetic manipulation of plants for virus resistance, pest resistance, herbicide
tolerance, resistance to fungi and bacteria.
ANIMAL BIOTECHNOLOGY
UNIT-III
Introduction to animal cell cultures
Requirement (laboratory equipment, media etc. primary and secondary culture cell lines)
Anchorage dependence and contact inhibition.
UNIT-IV
Cytodifferentation culturing of differentiation cells and retention of properties
Large scale production of animal cell in culture.
Transformation of animal cells. Transgenesis, applications of transgenic animal, Biofarming.
Stem cells : their applications in biology & medicine cloning : Procedure, applications &
problems.
16
B.Sc. 3rd Year, 6th Semester, Biotechnology (Elective)
Code
S.No. Course/Paper Theory Practical
Course No. Marks Course No. Marks
Environmental &
1. Fermentation BIOT-Elect-Sem-VI-T 75 BIOT-Elect-Sem-VI-/P 25
Biotechnology
Set nine questions in all in three sections. All questions carry equal marks.
Section A will cover Unit I & II
Set 2 questions from each unit and any 2 is to be attempted
Section – B will cover Unit – III & IV
Set 2 questions from each unit and any 2 is to be attempted
Section – C will be compulsory and will have 7 – 10 short answer type (not objective
type) questions covering the whole syllabus.
Objective:
ENVIRONMENTAL BIOTECHNOLOGY
UNIT-I
Introduction to Environment.
Renewable and Non Renewable resources. Conventional Fuels and their Environmental
Impacts.
Modern Fuels and their Environmental Impacts.
Methanogenic bacteria and biogas, microbial hydrogen production, conversion of sugars to
ethanol, The gasohol experiment, solar energy, converters – Hopes from the Photosynthetic
pigments.
Treatment of municipal waste and industrial effluents.
17
UNIT-II
FERMENTATION BIOTECHNOLOGY
UNIT-III
UNIT-IV
18
6. Isolation of Industrially important micro-organisms from soil samples.
7. Maintenance and culture of Saccharomyces cervisae in the laboratory.
8. Staining of S. cervisae.
9. Batch fermentation using shake flask for ethanol production.
10. Demonstration and working of the laboratory scale bioreactor.
**********
19