Beruflich Dokumente
Kultur Dokumente
ABSTRACT
Labeo calbasu is an important food fish and mrigala. The synonyms used for this species are
commonly known as the “calbasu” or “Black Rohu”. Cyprinus calbas (Hamilton,
Hamilton, 1822), Labeo
The present study was conducted on DNA extraction calbasu (Day, 1878), Labeo calbasu (Shaw and
and determination of DNA quantity of both male and Shebbeare, 1937). It is a popular food fish and also is
female of wild and cultured Labeo calbasu using admired as a sport fish. Recently this fish species has
Nanophotometer. DNA isolation was done and gel also made its entry in ornamental fish markets of India
electrophoresis was carried out. Extracted DNA was and abroad. In last few years, the natural populations
analyzed using nanophotometer (Nanophotometer of this fish species has seriously declined due to over
P330; Implen, Germany) to determine the fishing and other anthropological reasons.
concentration of DNA and its purity level. Several
different methods havee been employed in the The dorsal profile of Calbasu is more convex than of
quantification of nuclear DNA over the past 50 years. abdomen.Other
Other identifying features includes; Lips are
The ease of use of this technique not only makes it a thick and fringed. Two pairs of barbells present, rostal
feasible option for small volume analysis of DNA but pair longer than maxillary pair (Rahman, 1989).19 No
also a practical alternative for spectrophotometric pores on snout and eyeye situated a bit anterior from the
measurement. Significant
cant progress was made to half of the head (Bhuiyan, 1964).
964). Caudal peduncle is
measure micro volume liquid samples (<1µl) in short an lateral
ateral line well marked, scales are moderate
biotechnology & pharmaceutical applications. For the in size. There are 20 rows of scales before dorsal fin
present study both male and female of wild Labeo and 22 rows round the caudal peduncle (Bhuiyan,
calbasu (from middle Ganga Region) and cultured 1964). Mouth is moderately wide and also inferior.
Labeo calbasu (from culture ponds and/ hatchery) Gill openings of Kalibasu is wide and a gill rakers are
were taken. The value of DNA concentration in villiform, short and feeble (Bhuiyan, 1964). Colour of
female of wild Labeo calbasu was in between 58 -62 body is dark-black
black but the ventral part light dark. The
ng/µll and of male was in between range of 64 64- 68 ventral surface of the opercular region is white iris
ng/µl.
l. The value of DNA concentration in female of coppery. This fish attains a length of nearly 90 cm
cultured Labeo calbasu was 60- 66 ng/µ
ng/µl and of male (Talwar and Jhingran, 1991). Generally it is a bottom
68- 74 ng/µl. feeder. It feeds on vegetable matter, crustaceans,
insect larvae etc. Labeo calbasu feeds on algae 10%,
KEY WORDS: DNA Quantity, wild, cultured, higher plants 48%, protozoa 12%, crustacean 10%,
Nanophotometer, Labeo calbasu. mollusca 5%, mud and sand 15% (Bhuiyan, 1964). It
does inhabit rivers and tributaries. They are also seen
INTRODUCTION in deep pools clear sluggish streams, creeks. It can be
Labeo is a genus of carps in the family Cyprinidae. reared in ponds and also in tanks (Bhuiyan, 1964 and
Labeo calbasu locally called Kalibasu or Calbasu or Rahman, 1989).
Black rohu, is a freshwater fish species and is the
most important carp species next to three Indian Of wide distribution in India, it is one of the major
Major Carps; Labeo rohita, Catla catla and Cirrhinus Indian carps. It is an important food fish and at several
RESULTS
The present study was conducted on the isolation of
DNA and determination of DNA concentration of
both male and female of wild and cultured Labeo
calbasu using Nanophotometer.DNA quantification
was done by using nanophotometer and all data was
noted (Table 1). The value of DNA concentration in
female of wild Labeo calbasu was between 58 58-62 Fig. 2 Quantity of DNA content in Cultured L.
calbasu in Nanogram/ µl/individual
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Fig. 3 Comparative DNA Quantification in and agarose gel”.. A project report submitted to the
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female L. calbasu management, University of Agriculture, Nigeria,
Nigeria
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DNA measurement can provide valuable tool for
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DNA concentration in female of wild Labeo calbasu food Nut. Sci. 2014.
was between 58-62 ng/µll and of male was between
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ng/µl. Prado et. from different fish species based on a nuclear
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3041
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fisheries management, University of agriculture,
Nigeria determination of DNA concentration of 7. R. Verma, A. K. Singh, and K. Jaiswal,
Clarias gariepinus was done by nanodrop. DNA “Preliminary
Preliminary study on diminution level; of
quantification of Male and Female Clarias batrachus, RNA/DNA ratio in tissue of Labeo rohita by
Clarias gariepinus andClarias hybrids was also done exposure to some endocrine disrupting
by Shobhna (2017). She observed the he value of DNA compounds(EDCs)”, Aceh Journal of Animal
concentration in female of C. batrachus was in Science 1(1): 2016,pp 16-20.
20.
between 59 and 61 ng/µll and of male was in between 8. D. E. Ruzzante, C. T. Taggart, C. Cook, and
range of 70- 76 ng/µl. The value of DNA Goddard. (1996): Genetic differentiation between
concentration in female of C. gariepinus was 60 and inshore and offshore Atlantic cod Gadus morhua
63 ng/µl and of male 75- 78 ng/µl. µl. Highest DNA off new found land: microsatellite DNA variation
concentration was seen in hybrid individuals, in and antifreeze level. Canadian J.
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females the concentration was in between 7474-76 ng/µl 53: 634-645.