Beruflich Dokumente
Kultur Dokumente
HBV-1111
User’s Guide
BIONEER CORPORATION
8-11, Munpyeongseo-ro, Daedeok-gu,
Daejeon, 306-220, Republic of Korea
Tel: +82-1588-9788
Fax: +82-42-930-8600
Email: order@bioneer.co.kr
www.bioneer.com
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AccuPower® HBV Quantitative PCR Kit
Before, during and after use of this kit as described in this User’s
Guide, all potentially hazardous materials (i.e. materials that may have
come in contact with clinical samples) including tubes, tips and
materials should be processed and disposed of according to
applicable and appropriate regulations of the municipality/
government in which this product is being used.
Please read the User’s Guide before using this Kit. Please check the
integrity of all tubes, tips and other materials supplied with this kit
prior to use. Adhere to general clinical laboratory safety procedures
during the experiment.
Some applications that may be performed with this kit may infringe
upon existing patents in certain countries. The purchase of this kit
does not include or provide a license to perform patented
applications. Users may be required to obtain a license depending on
country and application. We do not condone nor recommend the
unlicensed use of a patented application.
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AccuPower® HBV Quantitative PCR Kit
BIONEER assumes liability under the condition that the user discloses
all information related to the problem to BIONEER in written form
within 30 days of occurrence.
Trademarks
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AccuPower® HBV Quantitative PCR Kit
TABLE OF CONTENTS
1. INTENDED USE……………………………………………..…………....6
2. INTRODUCTION……………………..…………………………..…….7
3. FEATURES AND PRINCIPLE OF THE TEST……….………….8
3.1. Kit Format………….………...……………………..…..........……....9
3.2. Sensitivity and Dynamic Range..………...………………..…...9
3.3. Internal Positive Control (IPC)……….………………………...9
4. CONTENTS OF THE KIT……………………………….……………..10
5. STORAGE………………….……………….……………………………..11
6. REQUIRED MATERIALS AND EQUIPMENT…………………....12
7. GENERAL PRECAUTIONS.……………………………………13
8. PROTOCOL….…………….………………………………………..…..14
8.1. Preparation: Specimen Collection, Storage, and
Transport…………………………………….………………………14
8.1.1. Specimen Collection.…………….….…………………….14
8.1.2. Sample Storage..…..……………………..……………………..14
8.1.3. Sample Transport………………………………………….15
8.1.4. Interfering Substances…………………………………….15
8.2. Work Flow Schema………..……………………………………..16
8.2.1. Nucleic Acid Extraction………..……...……………………...17
8.2.2. PCR Preparation………………………..…………..………..18
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AccuPower® HBV Quantitative PCR Kit
8.2.3. P r o g r a m m i n g t h e E x i c y c l e r ™ 9 6 R e a l - T i m e
Quantitative Thermal Block……………………...………….21
9. DATA ANALYSIS.….…….……………………………………………..27
9.1. Process of Data Analysis...……………………………...……..27
9.2. Analysis Results…………………………………...……….…31
10. TROUBLESHOOTING…………...…………………………………….33
11. REFERENCES …………………………………………………………..35
12. SYMBOLS…………………………………………………………….…...36
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AccuPower® HBV Quantitative PCR Kit
1. INTENDED USE
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AccuPower® HBV Quantitative PCR Kit
2. INTRODUCTION
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AccuPower® HBV Quantitative PCR Kit
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AccuPower® HBV Quantitative PCR Kit
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AccuPower® HBV Quantitative PCR Kit
Cat. No.
Labeling & Content
HBV-1111
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AccuPower® HBV Quantitative PCR Kit
5. STORAGE
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AccuPower® HBV Quantitative PCR Kit
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AccuPower® HBV Quantitative PCR Kit
7. GENERAL PRECAUTIONS
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AccuPower® HBV Quantitative PCR Kit
8. PROTOCOL
Do not store samples at 2~8℃ for over 7 day. For long term
storage of clinical samples, please aliquot and store at -20 ~ -
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AccuPower® HBV Quantitative PCR Kit
80℃.
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AccuPower® HBV Quantitative PCR Kit
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AccuPower® HBV Quantitative PCR Kit
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AccuPower® HBV Quantitative PCR Kit
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AccuPower® HBV Quantitative PCR Kit
⑨ To mix the tube thoroughly (to dissolve the Premix pellet and
mix the DNA), use the ExiSpin™ (Cat.No : A-7040, Bioneer
Corp., Korea) and perform 20 cycles at 2500 rpm for 5 sec.
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AccuPower® HBV Quantitative PCR Kit
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AccuPower® HBV Quantitative PCR Kit
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AccuPower® HBV Quantitative PCR Kit
Fig. 2) Side view and the 96-well thermal block of the instrument
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AccuPower® HBV Quantitative PCR Kit
⑤ Save the sample layout map file as an ‘*.xls’ file. Save the file
in an easily findable location.
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AccuPower® HBV Quantitative PCR Kit
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AccuPower® HBV Quantitative PCR Kit
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AccuPower® HBV Quantitative PCR Kit
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AccuPower® HBV Quantitative PCR Kit
9. DATA ANALYSIS
9.1. Process of Data Analysis
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AccuPower® HBV Quantitative PCR Kit
④ The HBV Quantitative PCR Kit tab will display the calculated
Ct, ΔRn values and validity decisions in spreadsheet format.
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AccuPower® HBV Quantitative PCR Kit
⑥ The STD Curve tab displays the standard curve derived from
the run standards.
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AccuPower® HBV Quantitative PCR Kit
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AccuPower® HBV Quantitative PCR Kit
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AccuPower® HBV Quantitative PCR Kit
Relative
Sample 50~108 IU/㎖ +
quantity
Not
Sample - +
Detected
Sample - - Repeat
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AccuPower® HBV Quantitative PCR Kit
10. TROUBLESHOOTING
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AccuPower® HBV Quantitative PCR Kit
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AccuPower® HBV Quantitative PCR Kit
11. REFERENCES
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AccuPower® HBV Quantitative PCR Kit
12. SYMBOLS
Temperature
Catalog Number
Limitation
Contains
Manufacturer
Sufficient for test
Caution, Consult
accompanying Batch code
documents
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AccuPower® HBV Quantitative PCR Kit
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