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ABO DISCREPANCIES
Principle:
A discrepancy exists when the result of red cell tests disagree with that of the serum
tests. When a discrepancy is encountered, it must be resolved. The interpretation is
delayed until testing and resolution is complete. If the clinical condition of the patient
makes it necessary to transfuse before the problem is resolved, only group 0 red cells
of the appropriate Rh type may be issued. If the problem cannot be resolved on site, a
specimen will be sent to the American Red Cross reference lab for resolution.
Procedure:
A. Initial tests
2. Wash the patient and/or reagent red cells several times and re-test.
a. Warm the serum and reagent red cells to 37C before mixing and
testing.
2. Anti-A, suspected
5. Subgroups
6. Acquired antigens
Reporting results:
1. All initial reactions are recorded as seen in the LIS per routine procedures, no
interpretation for the ABO group is entered until the discrepancy is resolved.
ADVENTIST HEALTHCARE LABORATORY SERVICES 4
SECTION: BLOOD BANK
PROCEDURE#111
Title: ABO DISCREPANCIES
3. Once resolved, the ABO group is interpreted in the LIS. An exception report
will generate because the front and back type disagree with the logic table
defined in the LIS. Enter a comment on the exception to explain any additional
testing performed.
Notes:
The AABB Technical Manual may be used as a reference for other specialized testing
related to ABO discrepancies.
References:
2. Standards for Blood Banks and Transfusion Services, AABB, 18th edition, 1997.
ABO DISCREPANCY WORKSHEET
Backtype at RT Backtype at 4C
Other:
Other:
Suspected anti-Al (A1 lectin typing of patient is ordered and resulted in LIS)
Al cells
A2 cells
0 cells
0 cells