BS411 No.

of Printed Pages – 2 BS411

3. Attempt any Two of the following questions: 7½ x 2 = 15 Roll No.
(a) Describe the initiation process of prokaryotic translation.
(b) Describe the structure of tRNA (only diagram). Explain the reaction M.Sc. (BIOTECHNOLOGY/BIOCHEMISTRY)
of tRNA charging. Name the enzyme which is involved in this
SECOND SEMESTER EXAMINATION, 2016-17
reaction. Also describe the properties of two classes of this
GENE EXPRESSION AND REGULATION
enzymes.
(c) Describe Trp operon with the help of suitable diagram. What do you
Time : 3 Hours Max. Marks : 75
understand by Attenuation? Explain.
Note : (i) Attempt ALL questions.
(ii) Choices are given in each question set.
4. Attempt any Two of the following questions: 7½ x 2 = 15
1. Attempt any Three of the following questions: 5 x 3 = 15
(a) What is Intein Splicing? Explain.
(a) Explain the formation of transcription initiation complex on pol II
(b) Distinguish and differentiate between the polycistronic and
promoter.
monocistronic RNAs.
(b) Describe the significance of RNA editing and mechanism of guide
(c) Describe the properties of genetic code. Also explain the
RNA directed RNA editing.
significance of isoacceptor tRNA and Wobble hypothesis.
(c) Explain the significance of nucleosome modifiers, mediator
complexes and chromatin remodellers.
5. Attempt any Two of the following questions: 7½ x 2 = 15
(d) Describe the structure of pol I and pol III promoters.
(a) What is the role of molecular chaperones in protein folding?
(b) Explain the role of EFT4 and peptidyle transferase in protein
2. Attempt any Three of the following questions: 5 x 3 = 15
synthesis.
(a) Describe the addition of Poly 'A' tail and its significance with respect
(c) Explain the process of expression of lac gene in presence of lactose
to the mRNA stability.
and in absence of glucose.
(b) Give the constituents of spliceosome and explain the process of
splicesome mediated splicing.
(c) Give an account of DNA binding motifs.
(d) Explain the steps involved in capping of mRNA and list the enzymes
involved in this process.

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