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ROLL NO:
YEAR: 2014-15
INDEX
CONTENTS PAGE NO.
1. Certificate of Authenticity
2. Acknowledgement
3. Aim of the project
4. Introduction
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CERTIFIC TE OF UTHENTICITY
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NCKNOWLEDGEMENT
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im of the Project
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Introduction
What is Antibiotic?
An antibiotic is an agent that either kills or inhibits the
growth of a microorganism.
The term antibiotic was first used in 1942 by Selman
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EXPERIMENTAL PROCEDURE
1. To 200ml
grams of distilled
of agar powder water
and 2ingrams
a flask,
ofIstarch.
added Then
8
putting a few pieces of dry hay into the medium I
covered the flask with an Inverted beaker. Boiling
the medium for 5 minutes and then cooling the
medium to room temperature. After that placing
the flask in a warm place. Within 2-3 days,
formation of scum of cloudy suspension appeared
on the medium indicating the growth of Bacillus
subtilis.
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dish.
coverRemoving the culture
of the Petridish. tube,
Moving theand replace
covered the
Petridish
along the table top to distribute the medium
evenly. Then I allowed the agar to cool. After that I
prepared two petridishes and marked them A & B.
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Petridishes
the bacteriaundistributed
to grow. TheninI warm place
observed theto allow
Petridishes for several days.
OBSERVATION:
CONCLUSION:
Antibiotic drugs killed most of the bacterial strain, hence
the areas appeared clear. However, a few strains which
were resistant in the bacterial population survived and
produced colonies later. This proves the resistant strain to
antibiotics were present in the bacterial population.
REFERENCES:
1. Comprehensive Laboratory Manual In Biology-XII
2. Biology Text For Class XII – NCERT
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SITES USED
1. http://www.wikipedia.org/
2. http://www.sciencedaily.com/articles/a/antibiotic_resistance.htm
3. http://www.betterhealth.vic.gov.au/bhcv2/bhcarticles.nsf/pages/Anti
biotic_resistant_bacteria
4. http://www.rxlist.com/antibiotic_resistance-page3/drugs-
condition.htm
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