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Advances in HPLC Stationary phases in Zirconia HPLC columns

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/ Advances in HPLC Stationary phases: Zirconia HPLC columns POSTED ON DECEMBER 6, 2013 BY DR.

POSTED ON DECEMBER 6, 2013 BY DR. DEEPAK BHANOT

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ON DECEMBER 6, 2013 BY DR. DEEPAK BHANOT 3 COMMENTS Advances in HPLC Stationary phases: Zirconia

Advances in HPLC Stationary phases: Zirconia HPLC columns

Advances in HPLC Stationary phases: Zirconia HPLC columns Silica-based HPLC columns have remained workhorses in

Silica-based HPLC columns have remained workhorses in HPLC laboratories. Over the years with advances in particle technologies size reduction has been possible from 5μm to sub- 2μm which led to development of UHPLC. Further introduction of Core Shell Technologies and monolithic columns extended the range of applications and contributed to larger sample throughputs.

Properties of Zirconia
Properties
of
Zirconia

supports

The main limitation of silica columns is availability of pH range between 2 and 8 and temperature to ambient. On the other hand Zirconia supports exhibits operational stability over the entire pH range and working temperatures up to 200°C.

Lewis acid
Lewis acid
pH range and working temperatures up to 200°C. Lewis acid Monodisperse, Spherical and Nonporous Zirconia Particles

Monodisperse, Spherical and Nonporous Zirconia Particles

Zirconia or ZrO2 is an oxide in which the Zr atom acts as a Lewis acid i.e. – it accepts electron pair due to prescence of empty orbital. This feature provides enhanced selectivity over conventional silica columns.

Adsorption capacity
Adsorption capacity

Adsorption capacity is larger in comparison to silica packings

Chemical resistance
Chemical resistance

High resistance to acidic and basic solutions. Retention of basic and acidic analytes can be nely controlled by changing the pH of medium over the entire available range.

Types of phases
Types of phases

Four surface chemistries based on surface activities are available which greatly extend selectivity and retention of for improved separations

Polybutadiene coated Zirconia
Polybutadiene coated Zirconia

The phase is similar to reverse phase using C18 silica but has following advantages:

Available pH range 1 – 13reverse phase using C18 silica but has following advantages: Temperature stability up to 200°C Less hydrophobic

Temperature stability up to 200°Cbut has following advantages: Available pH range 1 – 13 Less hydrophobic than C18 and is

Less hydrophobic than C18 and is suitable for separations involving amines and basespH range 1 – 13 Temperature stability up to 200°C SEARCH THIS WEBSITE … POPULAR LATEST

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Advances in HPLC Stationary phases in Zirconia HPLC columns

Zirconia-C18

Porous carbon clad zirconia particles covalently modi ed with C18 groups. ed with C18 groups.

Selectivity is rather di erent from conventional C18 columns erent from conventional C18 columns

Can be used over the entire pH range (1 – 14) alongwith high temperature capabilities (up tois rather di erent from conventional C18 columns 200°C) Zirconia – polystyrene Porous Zirconia particles

200°C)

Zirconia – polystyrene
Zirconia – polystyrene

Porous Zirconia particles are modi ed with cross-linked polystyrene. ed with cross-linked polystyrene.

Tolerate strongly aqueous solutions up (up to 100% pure water)particles are modi ed with cross-linked polystyrene. Operational pH range 1 – 13 Useful for separation

Operational pH range 1 – 13strongly aqueous solutions up (up to 100% pure water) Useful for separation of hydrophobic compounds and

Useful for separation of hydrophobic compounds and aminesup (up to 100% pure water) Operational pH range 1 – 13 No requirement for ion

No requirement for ion pairing requirement ion pairing requirement

Reduced tailing of basic compounds in comparison to silica-based packingsand amines No requirement for ion pairing requirement Provides mixed mode Reverse phase and Ion exchange

Provides mixed mode Reverse phase and Ion exchange separationsof basic compounds in comparison to silica-based packings Zirconia-Carbon Porous carbon clad Zirconia particles

Zirconia-Carbon

Porous carbon clad Zirconia particles provide enhanced retention of polar compoundsReverse phase and Ion exchange separations Zirconia-Carbon Highly hydrophilic surface Useful for separation of isomers

Highly hydrophilic surfaceparticles provide enhanced retention of polar compounds Useful for separation of isomers and diastereomers Good

Useful for separation of isomers and diastereomersretention of polar compounds Highly hydrophilic surface Good retention of fused – ring aromatic compounds Column

Good retention of fused – ring aromatic compoundssurface Useful for separation of isomers and diastereomers Column care Contaminated columns can deteriorate the peak

Column care
Column care

Contaminated columns can deteriorate the peak shape, e ect retention times, lead to high backpressure and increase baseline noise. Use of concentrations above 25 mM phosphate, acetate, carbonate/bicarbonate bu ers and salts should be avoided. Anions such as carboxylic acids, uoride and phosphate strongly absorb on Zirconia columns. The recommended steps for column regeneration are

1. Flush with a 20:80 mixture of acetonitrile and pH 10 ammonium hydroxide for 50 column volumes followed by 10 column volumes of water

2. Repeat with 20:80 mixture of acetonitrile and 0.1 M nitric acid for same column volumes

3. Flush with pure methanol, acetonitrile, isopropanol or tetrahydrofuran for 20 column volumes. Zirconia HPLC columns will require the same solvents with at least 20% THF

Storage

Zirconia HPLC columns should not be stored in phosphate bu er. The recommended storage procedure is er. The recommended storage procedure is

Overnight – 10 volumes of organic modi er/water er/water

Long-term storage – 10 volumes of pH 10 ammonium hydroxide followed by 10 volumes of 50: 50 organic modi er/water er/water

We have seen that zirconia-based reverse phases o er a great choice of selectivity and retention. Additional bene ts of column chemistries, pH and temperature extremes contribute to versatility of applications. A subsequent article on monolith stationary phase packing will introduce you to further advances in stationary phases.

Please share your views and leave comments on the article

Filed Under: HPLC

views and leave comments on the article Filed Under: HPLC About Dr. Deepak Bhanot Dr Deepak
About Dr. Deepak Bhanot
About Dr. Deepak Bhanot

Dr Deepak Bhanot is a seasoned professional having nearly 30 years expertise beginning from sales and product support of analytical instruments. After

completing his graduation and post graduation from Delhi University and IIT Delhi he went on to Loughborough University of Technology, UK for doctorate research in analytical chemistry. His mission is to develop training programs on analytical techniques and

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Advances in HPLC Stationary phases in Zirconia HPLC columns

share his experiences with broad spectrum of users ranging from professionals engaged in analytical development and research as well as young enthusiasts fresh from academics who wish to embark upon a career in analytical industry.

Comments

to embark upon a career in analytical industry. Comments Meinster Kodjo Eduafo says: February 25, 2017
to embark upon a career in analytical industry. Comments Meinster Kodjo Eduafo says: February 25, 2017

Meinster Kodjo Eduafo says:

February 25, 2017 at 2:51 am

Dear Dr. Deepak

I think this is a great breakthrough for HPLC analysis. please can send me a quotation for each of them. I will be very grateful. Thank you very much for this insight.

Best regards

Reply

Thank you very much for this insight. Best regards Reply Dr. Deepak Bhanot says: February 25,
Thank you very much for this insight. Best regards Reply Dr. Deepak Bhanot says: February 25,

February 25, 2017 at 9:49 am

Please specify your requirement.You may subscribe to our on- line training programs but let me clarify that we are not a supplier of columns or any chromatographic consumables.

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