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28/04/2019

Cost Saving Tips for the HPLC User

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POSTED ON JANUARY 31, 2014 BY DR. DEEPAK BHANOT

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Cost Saving Tips for the HPLC User
Cost Saving Tips for the HPLC User

HPLC analysis involves the use of expensive mobile phase solvents and recurring costs on consumables such as columns, sample and solvent lters, vials, glassware, etc.

The costs involved in HPLC operation can be grouped in terms of laboratory throughput and

operational costs. The objective of this article is to o er some useful tips to reduce costs without

sacri cing the quality of results.

Time saved means money saved
Time saved means money saved

Time-saving is the essence of high laboratory

throughput which translates to saving of money. At

the end of the day, timely completion of activities

additionally gives you greater con dence and work

satisfaction.

Keep required glassware ready in advance as pergives you greater con dence and work satisfaction. the planned analysis schedule. Ensure availability of HPLC

the planned analysis schedule.

Ensure availability of HPLC solvents and HPLC solvents and

standards for the planned analysis.

Ensure leak-free connections to avoidof HPLC solvents and standards for the planned analysis. termination of runs. Adopt automated injection systems

termination of runs.

Adopt automated injection systems when a largeEnsure leak-free connections to avoid termination of runs. number of samples are to be analyzed. Automated

number of samples are to be analyzed.

Automated systems further reduce personal

errors.

analyzed. Automated systems further reduce personal errors. Cost Saving Tips for the HPLC User SEARCH THIS

Cost Saving Tips for the HPLC User

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Use only calibrated and validated instruments and methods to avoid the need for repeatJetpack plugin with Stats module needs to be enabled. analysis. TOPICS Keep a stock of essential

analysis.

TOPICS

Keep a stock of essential recommended spares to prevent time-consuming breakdowns.and methods to avoid the need for repeat analysis. TOPICS Users should be made aware of

Users should be made aware of preventive maintenance and troubleshooting guidelines.

Columns required for the HPLC analysis should be kept ready in advance after requiredof preventive maintenance and troubleshooting guidelines. conditioning and washing cycles. Operational Costs Mobile

conditioning and washing cycles.

Operational Costs
Operational Costs

Mobile phase solvents are the major cost contributors in HPLC analysis. Analysis of therequired conditioning and washing cycles. Operational Costs samples requiring the use of same mobile phase and

samples requiring the use of same mobile phase and column should be grouped together

as far as possible.

Consumables such as sample and mobile solvent lters should be discarded after use. Do lters should be discarded after use. Do

not attempt to save cost by reusing lters.

Vials for sample collection can be reused only after washing and drying as per prescribedafter use. Do not attempt to save cost by reusing lters. procedures. Lower the mobile phase

procedures.

Lower the mobile phase ow rate to the minimum in between analysis. ow rate to the minimum in between analysis.

In case HPLC columns are to be used after a long interval wash with wash solvents followedmobile phase ow rate to the minimum in between analysis. by storage solvent with end caps

by storage solvent with end caps xed. This will ensure that the columns will operate under

required separation e ciencies and save cost on frequent column replacements

Check HPLC column performance in between analysis using injections of standards.e ciencies and save cost on frequent column replacements Your suggestions and views based on your

Your suggestions and views based on your personal experiences are welcome so please leave

your comments.

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28/04/2019

Cost Saving Tips for the HPLC User

Filed Under: HPLC

Cost Saving Tips for the HPLC User Filed Under: HPLC Tagged With: High-performance Liquid Chromatography About
About Dr. Deepak Bhanot
About Dr. Deepak Bhanot

Dr Deepak Bhanot is a seasoned professional having nearly 30 years expertise beginning from sales and product support of analytical instruments. After

completing his graduation and post graduation from Delhi University and IIT Delhi he went on to Loughborough University of Technology, UK for doctorate research in analytical chemistry. His mission is to develop training programs on analytical techniques and share his experiences with broad spectrum of users ranging from professionals engaged in analytical development and research as well as young enthusiasts fresh from academics who wish to embark upon a career in analytical industry.

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