1

The _______ of a compound light microscope focuses light from the light source on the
specimen.
Condenser lens OR condenser
2
Which of the following is the most convenient and appropriate unit for expressing the
size of an average bacterial cell?
Micrometer
3
All of the following are visible through a compound light microscope except____.
0.02-AUM ribosome
4
Bacterial motility and flagellar movement can readily be viewed using transmission
electron microscopy.
False
5
A____________ is equal to 0.000001m.
micrometer
6
Acid-fast Mycobacteria differ from non-acid-fast bacteria by the presence of ____. In the
decolorizing step of the acid -fast stain, which reagent is used?
carbol fuchsin waxy material in their cell wall
7
When stained, bacteria in the genus Mycobacterium are ____. When using a 10x ocular
lens and a 40x objective, the total magnification achieved is
acid-fast 400x
8
Which of the following is not a modification of a compound microscope?
electron microscope
9
Flagella are too ________ to be seen with the light microscope without adding a mordant.
small or thin
10
Immersion oil acts to decrease refraction of light rays and thus increase resolution.
True
11
In the Gram stain, if the decolorizing step is deleted, gram-negative cells will appear
_______ at the completion of the staining procedure.
Purple
12
Magnification is the quality of the microscope that allows on to distinguish between two
points that are very close together.
False
13
Place these structures of the compound light microscope in order that light passes
through them on the way to the observer's eyes: (1) condenser,(2)ocular lens, (3)
illuminator, (4)specimen, (5) objective lens.
3-1-4-5-2
Response feedback: The path of light begins with the illuminator, then passes through
the condenser, the specimen, the objective lens, then then the ocular lens.
14
Bacterial smears must be heat-fixed before all staining procedures.
False
15
The ______ charge of a basic dye adheres to the ______ charge of bacterial cell surfaces.
positive;negative
16
The _____ is the part of the microscope that controls the amount of the light entering the
condenser.
diaphragm
17
The Gram stain is important in medical microbiology because the results help physicians
select appropriate antibiotics for treatment.
True
18
Which of the following kinds of microscopy would be most appropriate for viewing the
shape and arrangement of pili or fimbriae on the surface of the bacterial cell?
scanning electron microscopy
19
Which of the following types of microscopy is most useful for viewing the internal
structures of unstained specimens?
Phase-contrast
20
You are viewing a sputum smear that has been stained with an acid-fast stain. On this
smear you see 5-Aum-long red cells. You can conclude that _______.
there are acid-fast bacteria in the specimen