Instrument:HPLC-UQ Sequence:Secuencia ID cafeina FITO II Vi. 2.

5 microlitros Page 1 of 4

Peak Analysis
Injection Details
Injection Name: Estandar Cafeína Run Time (min): 8.00
Vial Number: RA2 Injection Volume: 2.50
Injection Type: Unknown Channel: UV_VIS_1
Calibration Level: Wavelength: n.a.
Instrument Method: Grupo 2 cafeína Bandwidth: n.a.
Processing Method: New Processing Method Dilution Factor: 1.0000
Injection Date/Time: 16/abr/19 08:49 Sample Weight: 1.0000

Chromatogram

Peak Results
No. Peak Name Retention Time Width (50%) Type Resolution (EP) Asymmetry (EP) Plates (EP)
min min
1 CAFEINA 4.777 0.185 M n.a. 2.36 3685
2 Component 2 5.423 n.a. Rd n.a. n.a. n.a.
3 Component 3 6.143 n.a. M n.a. n.a. n.a.

Chromeleon (c) Dionex

Default DAD/Peak Analysis Version 7.2.0.3765
Instrument:HPLC-UQ Sequence:Secuencia ID cafeina FITO II Vi. 2.5 microlitros Page 2 of 4

Spectral Results
Injection Details
Injection Name: Estandar Cafeína Run Time (min): 8.00
Vial Number: RA2 Injection Volume: 2.50
Injection Type: Unknown Channel: UV_VIS_1
Calibration Level: Wavelength: n.a.
Instrument Method: Grupo 2 cafeína Bandwidth: n.a.
Processing Method: New Processing Method Dilution Factor: 1.0000
Injection Date/Time: 16/abr/19 08:49 Sample Weight: 1.0000

Contour Plot

Peak Purity
No. Peak Name Ret.Time Match RSD Match PPI RSD PPI nExtrema
min % nm %
1 CAFEINA 4.777 1000 0.01 219.8 0.09 2
2 Component 2 5.423 n.a. n.a. n.a. n.a. 80
3 Component 3 6.143 985 2.13 221.1 0.93 128

Chromeleon (c) Dionex

Default DAD/Spectral Results Version 7.2.0.3765
Instrument:HPLC-UQ Sequence:Secuencia ID cafeina FITO II Vi. 2.5 microlitros Page 3 of 4

Spectral Library Screening Results

Injection Details
Injection Name: Estandar Cafeína Run Time (min): 8.00
Vial Number: RA2 Injection Volume: 2.50
Injection Type: Unknown Channel: UV_VIS_1
Calibration Level: Wavelength: n.a.
Instrument Method: Grupo 2 cafeína Bandwidth: n.a.
Processing Method: New Processing Method Dilution Factor: 1.0000
Injection Date/Time: 16/abr/19 08:49 Sample Weight: 1.0000

UV Spectra n.a.

Spectral Library Screening Results

Hit# 1 Hit# 2 Hit# 3
Library: n.a. n.a. n.a.
Component: n.a. n.a. n.a.
Match Factor: n.a. n.a. n.a.
Ret.Time: n.a. n.a. n.a.
Origin:
Acq.Date: n.a. n.a. n.a.
Instrument Method: n.a. n.a. n.a.

Chromeleon (c) Dionex

Default DAD/Spectral Library Screening Version 7.2.0.3765
Instrument:HPLC-UQ Sequence:Secuencia ID cafeina FITO II Vi. 2.5 microlitros Page 4 of 4

Chromatogram

Chromeleon (c) Dionex

Default DAD/Chromatogram Version 7.2.0.3765