Uniprot (practicle)

Just follow the website for the protein information of any gene.here am going to search for some liver
protein genes of homo sapiens.steps are as followd:

1. Come on uniprot home page

 http://www.uniprot.org/
2. select (protein knowledgebase)in search in bar,write in text field(query) thet search name(some
liver protein genes homo sapiens)
3. select one of result,
 http://www.uniprot.org/uniprot/?query=liver+protein+in+homo+sapiens&sort=score
4. selected result will show many features,certains are,
 http://www.uniprot.org/uniprot/P78556

1st gene

Accession: P78556

Protein names: C-C motif chemokine 20

Gene names: CCL20

Function: Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not
monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be
involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and
dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally
chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus
ATCC 29213.

Tissue specificity: Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood
lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon.

Subcellular location: Secreted.

5. Now select fasta file formate

 http://www.uniprot.org/uniprot/P78556.fasta

MCCTKSLLLAALMSVLLLHLCGESEAASNFDCCLGYTDRILHPKFIVGFTRQLANEGCDI
NAIIFHTKKKLSVCANPKQTWVKYIVRLLSKKVKNM
6. Now take protein name and search for neucleotide sequence of it.on its home page
 http://www.ncbi.nlm.nih.gov/guide/
7. then select a mRna sequence
 http://www.ncbi.nlm.nih.gov/nuccore/AY890601.1

ACCESSION: AY890601

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ATGTGCTGTACCAAGAGTTTGCTCCTGGCTGCTTTGATGTCAGTGCTGCTACTCCACCTCTGCGGCGAAT
CAGAAGCAAGCAACTTTGACTGCTGTCTTGGATACACAGACCGTATTCTTCATCCTAAATTTATTGTGGG
CTTCACACGGCAGCTGGCCAATGAAGGCTGTGACATCAATGCTATCATCTTTCACACAAAGAAAAAGTTG
TCTGTGTGCGCAAATCCAAAACAGACTTGGGTGAAATATATTGTGCGTCTCCTCAGTAAAAAAGGCAAGA
ACATGTAG

2nd gene
 http://www.uniprot.org/uniprot/P07148

Accession: P07148

Protein names: Fatty acid-binding protein, liver

Gene names: FABP1

Function: Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other
small molecules in the cytoplasm. May be involved in intracellular lipid transport.

Subcellular location: Cytoplasm

 http://www.uniprot.org/uniprot/P07148.fasta

MSFSGKYQLQSQENFEAFMKAIGLPEELIQKGKDIKGVSEIVQNGKHFKFTITAGSKVIQ
NEFTVGEECELETMTGEKVKTVVQLEGDNKLVTTFKNIKSVTELNGDIITNTMTLGDIVF
KRISKRI

Ncbi

 http://www.ncbi.nlm.nih.gov/nuccore/XM_532966.2

ACCESSION XM_532966

CAGAGCTGCAGGTCCACTGTGGAGGGAGCCTTGTTGCCACCATGAACTTCTCCGGCAAGTACCAACTGCA
GAGCCAAGAAAACTTTGAGGCCTTCATGAAGGCAGTCGGTCTGCCTGATGAACTCATCCAGAAGGGGAAG
GACCTCAAGGGGGTGTCAGAAATCGTGCAGAATGGGAAGCACTTCAAGCTCATCATCACCACTGGTTCCA
AAGTGATCCAGAATGAGTTCACCTTGGGGGAAGAGTGTGAGCTGGAGACCATGACTGGAGAGAAGGTCAA
GGCAGTGGTTCAGATGGAAGGTGACAATAAGCTGGTGACAACTTTCAAAGGCATCAAGTCTGTGACCGAA
CTCAATGGCGATGTGATCACCAATACCATGACATTGGGTGACATTGTCTTCAAGAGAATCAGCAAGAGAA
TTTAGACAAGTCTGCACTTCATATTCTTTTACTGTGTAAAATTAATGTAATAAAATGAACTTTGTTTTAA
A
rd
3 gene

 http://www.uniprot.org/uniprot/Q93096

Accession: Q93096

Protein names: Protein tyrosine phosphatase type IVA 1

Gene names: PTP4A1

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 Uniprot (practicle)

Function: Protein tyrosine phosphatase which stimulates progression from G1 into S phase
during mitosis. May play a role in the development and maintenance of differentiating epithelial
tissues. Enhances cell proliferation, cell motility and invasive activity, and promotes cancer
metastasis

Subcellular location: Cell membrane. Early endosome. Endoplasmic reticulum. Cytoplasm.

Cytoplasm › cytoskeleton › spindle.

 http://www.uniprot.org/uniprot/Q93096.fasta

MARMNRPAPVEVTYKNMRFLITHNPTNATLNKFIEELKKYGVTTIVRVCEATYDTTLVEK
EGIHVLDWPFDDGAPPSNQIVDDWLSLVKIKFREEPGCCIAVHCVAGLGRAPVLVALALI
EGGMKYEDAVQFIRQKRRGAFNSKQLLYLEKYRPKMRLRFKDSNGHRNNCCIQ
Ncbi

 http://www.ncbi.nlm.nih.gov/nuccore/AY890582.1

ACCESSION AY890582
 http://www.ncbi.nlm.nih.gov/nuccore/61364316?report=fasta

ATGGCTCGAATGAACCGCCCAGCTCCTGTGGAAGTCACATACAAGAACATGAGATTTCTTATTACACACA
ATCCAACCAATGCGACCTTAAACAAATTTATAGAGGAACTTAAGAAGTATGGAGTTACCACAATAGTAAG
AGTATGTGAAGCAACTTATGACACTACTCTTGTGGAGAAAGAAGGTATCCATGTTCTTGATTGGCCTTTT
GATGATGGTGCACCACCATCCAACCAGATTGTTGATGACTGGTTAAGTCTTGTGAAAATTAAGTTTCGTG
AAGAACCTGGTTGTTGTATTGCTGTTCATTGCGTTGCAGGCCTTGGGAGAGCTCCAGTACTTGTTGCCCT
AGCATTAATTGAAGGTGGAATGAAATACGAAGATGCAGTACAATTCATAAGACAAAAGCGGCGTGGAGCT
TTTAACAGCAAGCAACTTCTGTATTTGGAGAAGTATCGTCCTAAAATGCGGCTGCGTTTCAAAGATTCCA
ACGGTCATAGAAACAACTGTTGCATTCAATAG

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