UTILIZATION OF POTATO (SOLANUM

TUBEROSUM ) EXTRACT IN A NATURAL

ACNE REDUCING OINTMENT
BOTENGAN, Debra Jannine F.
CHUA, Alfred Apten U.
DOM-OGEN, Kirk Patrick T.
PASI, Hannah Kate E.
TAYAB, Hazel Keith O.
INTRODUCTION
RATIONALE OF THE STUDY
 Acne vulgaris or Acne significantly affects physical and psychosocial well-being
-Dawn et al. (2007)
-Adiki and Pranay (2012), as cited by Sekar and Halim (2017)

 Large amount of chemicals present in most anti-acne products causes side-effects

-Sekar and Halim (2017)

Insight on alternative ways to reduce acne

 PARADIGM OF THE STUDY

INPUT PROCESS OUTPUT

Experimentation
Creation of ointment through
Potato Extract fusion method
Ointment Base (Coconut oil and Observation and evaluation
beeswax) Antibacterial test Natural acne reducing ointment
 STATEMENT OF THE PROBLEM:
1. What is the antibacterial activity of Solanum tuberosum extract against acne causing bacteria:
a. Staphylococcus aureus
b. Escherichia coli

2. What are the physical properties of the formulated ointments with different potato extract concentrations in terms of:
a. Texture
b. Odor
c. Wash ability
d. Irritancy
STATEMENT OF THE PROBLEM 1:
1. What is the antibacterial activity of Solanum tuberosum extract against acne causing bacteria:
a. Staphylococcus aureus
b. Escherichia coli

HYPOTHESIS POPULATION AND LOCALE RESULT

Held at CAS, Microbiology
Room
-Antibacterial Test Result
METHOD USED
The potato extract will have (Zone of Inhibition)
an antibacterial activity as Disk-Diffusion Method
indicated by the presence of
zone of inhibition. TOOL USED
T-test
 ANTIBACTERIAL TEST RESULTS
       
       
       
       
       

       
       
       
 

Bacterium Zone of Inhibition Total Average

Disk 1 Disk 2 Disk 3

         
         
         
         

Staphylococcus aureus
         

8 mm 8 mm 8 mm 24 mm 8 mm

 
 

 9 mm
         
         
         
       

Escherichia coli
     

10 mm 9 mm 8 mm 27 mm
STATEMENT OF THE PROBLEM 1:
1. What is the antibacterial activity of Solanum tuberosum extract against acne causing bacteria:
a. Staphylococcus aureus
b. Escherichia coli

HYPOTHESIS POPULATION AND LOCALE RESULT CONCLUSION

The potato extract will have
an antibacterial activity as
indicated by the presence of
zone of inhibition.
Held at CAS, Microbiology Room
METHOD USED
Disk-Diffusion Method Antibacterial Test Result -Solanum tuberosum extract is
resistant against common acne-
(Zone of Inhibition) causing bacteriaStaphylococcus
TOOL USED aureus andEscherichia coli
T-test T-test Result

RECOMMENDATION
1. Determine what specific component
2. Different methods ofofplant
the potato extract
extraction allows it to
(aqueous, be resistant
ethanol, against Staphylococcus
and methanol extraction) aureus and
Escherichia coli
 STATEMENT OF THE PROBLEM 2:
2. What are the physical properties of the formulated ointments with different potato extract concentrations in terms
of texture, odor, wash ability, and irritancy?

HYPOTHESIS METHOD USED

Making of Ointments (Fusion
Method)
There is a significant
difference in the output of Evaluation (Convenience Sampling)
the experiments.
POPULATION AND LOCALE
30 Respondents
TOOL USED
One-way ANOVA
Components of the different ointment formulations

     
  Treatment/Formula A Treatment/Formula B Treatment/Formula C
Components      
(20% concentration) (40% concentration) (50% concentration)

       
Potato Extract 10g 25g 45g

       
Coconut Oil 30g 30g 30g

       
Beeswax 8g 8g 8g

       
Tea Tree Oil 5 mL 5 mL 5 mL
Creation of Ointments
 STATEMENT OF THE PROBLEM 2:
2. What are the physical properties of the formulated ointments with different potato extract concentrations in terms
of texture, odor, wash ability, and irritancy?

HYPOTHESIS METHOD USED RESULT

Making of Ointments
There is a significant Evaluation (Convenience Sampling) Physical Evaluation of the Formulated
difference in the output of Ointments Result
the experiments. POPULATION AND LOCALE
30 Respondents
TOOL USED
One-way ANOVA
 Texture of Formulated Ointments
TEXTURE M DE F P-value
F1 4.17 S 1.3539 0.2624

F2 4.13 S    
F3 4.37 VS    
Overall 4.22 VS    

Scale Descriptive Equivalent

4.21 - 5.0 Very smooth (VS)
3.41 - 4.20 Smooth (S)
2.61 - 3.40 Acceptable (A)
1.81 - 2.60 Rough (R)
1 – 1.80 Very rough (VR)
 Odor of Formulated Ointments
ODOR M DE F P-value

F1 3.40 O 0.0269 0.9734

F2 3.37 O    

F3 3.43 P    

Overall 3.40 O    

Scale Descriptive Equivalent

4.21 - 5.0 Very pleasant (VP)
3.41 - 4.20 Pleasant (P)
2.61 - 3.40 Odorless (O)
1.81 - 2.60 Unpleasant (U)
1 – 1.80 Very unpleasant (VU)
 Wash Ability of Formulated Ointments

WASH ABILITY M DE F P-value

F1 3.73 G 0.0630 0.9389

F2 3.77 G    
F3 3.83 G    
Overall 3.78 G    

Scale Descriptive Equivalent

4.21 - 5.0 Very good (VG)
3.41 - 4.20 Good (G)
2.61 - 3.40 Acceptable (A)
1.81 - 2.60 Poor (P)
1 – 1.80 Very poor (VP)
 Irritancy of Formulated Ointments

IRRITANCY M DE F P-value

F1 4.80 NI 0.2863 0.7517

F2 4.77 NI    
F3 4.87 NI    
Overall 4.81 NI    

Scale Descriptive Equivalent

4.21 - 5.0 Non-irritant (NI)
3.41 - 4.20 Slightly irritant(SI)
2.61 - 3.40 Moderately Irritant (MI)
1.81 - 2.60 Very Irritant (VI)
1 – 1.80 Extremely Irritant (EI)
 STATEMENT OF THE PROBLEM 2:
2. What are the physical properties of the formulated ointments with different potato extract concentrations in terms
of texture, odor, wash ability, and irritancy?

HYPOTHESIS METHOD USED RESULT

Making of Ointments
There is a significant Evaluation (Convenience Sampling) Physical Evaluation of the Formulated
difference in the output of Ointments Result
the experiments. POPULATION AND LOCALE
30 Respondents
General Acceptability of the Formulated
TOOL USED Ointments
One-way ANOVA
General Acceptability of Formulated Ointments

GA M DE F P-value

F1 4.03 SA 0.7149 0.4920

F2 4.13 SA    
F3 4.17 SA    
Overall 4.07 SA    

Scale Descriptive Equivalent

4.21 - 5.0 Very acceptable (VA)
3.41 - 4.20 Slightly acceptable (SA)
2.61 - 3.40 Neutral (N)
1.81 - 2.60 Slightly unacceptable (SU)
1 – 1.80 Unacceptable (U)
 STATEMENT OF THE PROBLEM 2:
2. What are the physical properties of the formulated ointments with different potato extract concentrations in terms
of texture, odor, wash ability, and irritancy?

HYPOTHESIS METHOD USED RESULT CONCLUSION

Making of Ointments
There is a significant Evaluation (Convenience Sampling)
difference in the output of
the experiments. POPULATION AND LOCALE
30 Respondents
TOOL USED
One-way ANOVA
Physical Evaluation of the Formulated
Ointments Result
General Acceptability of the Formulated
Ointments
There is no significant
difference between the physical
properties and general
acceptability of the formulation
of ointments.

RECOMMENDATION
-The amount of the other components of the ointments may vary.
Salamat adehhh